LOCUS       pFC543                  5111 bp    DNA     circular SYN 14-AUG-2020
DEFINITION  cHS4-[gRNA1]miniCMV-Gal4-VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(25..30)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(95..955)
                     /label=amp marker
                     /note="Geneious type: marker"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    98..955
                     /label=AmpR
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    793..798
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(810..815)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(997..1025)
                     /label=amp prom
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1102..1146
                     /label=seq1
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1724
                     /label=pBjh_3260 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=pL0 I
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1147..1150
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1151..1720
                     /label=2x cHS4 Insulator
                     /note="Geneious type: Insulator"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1229..1233
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     1379..1397
                     /label=FCS1
                     /note="color: black; sequence: TCTTTGAGCCTGCAGACAC"
     protein_bind    1508..1512
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     1658..1676
                     /label=FCS1
                     /note="color: black; sequence: TCTTTGAGCCTGCAGACAC"
     misc_feature    1721..1950
                     /label=PCR861 Correct (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=TACT
                     /note="Geneious type: protein_bind"
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1721..1724
                     /label=pL0 I-P
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1725..1744
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1745..1747
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1766..1785
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1786..1788
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1807..1826
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1827..1829
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1848..1867
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1868..1870
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     gene            1871..1946
                     /label=miniCMV
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1871..1933
                     /label=miniCMV
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1929
                     /label=Transcription starting point
                     /note="Geneious type: DNA probe bind"
                     /note="/Sequence=A"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1947..1974
                     /label=L0.5.2_Inert (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     unsure          1947..1950
                     /label=CAGA
                     /note="/created by=fabio"
                     /note="color: #a6acb3"
     misc_feature    1947..1950
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1947..1950
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     5'UTR           1951..1970
                     /label=Inert
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..4123
                     /label=pBjh_4067 (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..1974
                     /label=pL0 5-G
                     /note="Geneious type: Gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..1974
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..1974
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1971..1974
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1975..1980
                     /label=Kozak
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     1979..2002
                     /label=JH1716F
                     /note="color: black; sequence: 
                     ATGGGGTACCCATACGACGTGCCAGATTACGCCATGAAGCTACTGTCCTCTATCG"
     CDS             1981..4119
                     /label=Gal4-NLS-VPR
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1984..2421
                     /label=Gal4 DBD
                     /note="Geneious type: domain"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2011..2094
                     /label=Zn(2)-C6 fungal-type.{ECO:0000255|PROSITE-ProRu...
                     /note="Geneious type: dna_binding_domain"
                     /note="/Transferred_From=Gal4 DBD (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2086..2104
                     /label=FCS2
                     /note="color: black; sequence: TGGGAGTGTCGCTACTCTC"
     primer_bind     2425..2445
                     /label=JH1711F
                     /note="color: black; sequence: 
                     ATGGGGTACCCATACGACGTGCCAGATTACGCCGGCCCCAAGAAGAAGAGGAAG"
     misc_feature    2428..2448
                     /label=SV40 NLS
                     /note="Geneious type: motif"
                     /note="/Transferred_From=Digestion of pRJ_00009 PCR Product
                     - Fragment 2, Digestion of pRJ_00010 PCR Product - Fragment
                     2, Digestion of pRJ_00011 PCR Product - Fragment 2, 
                     Digestion of pRJ_00012 PCR Product - Fragment 2, Digestion 
                     of pRJ_00013 PCR Product - Fragment 2, pRJ_00009, pRJ_00009
                     PCR Product, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, 
                     pRJ_00011 PCR Product, pRJ_00012, pRJ_00012 PCR Product, 
                     pRJ_00013, pRJ_00013 PCR Product, pRJ_00095, pRJ_00096, 
                     pRJ_00097, pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2439..2458)
                     /label=RJ_00096Ra
                     /note="color: black; sequence: 
                     GGCGTAATCTGGCACGTCGTATGGGTAGCCAGATCCCACCTTCCTC"
     primer_bind     2449..2474
                     /label=RJ_00096Fb
                     /note="color: black; sequence: 
                     GTGCCAGATTACGCCGGATCAGGTTCGCCAGGGATCCGTCG"
     misc_feature    2449..2457
                     /label=GSG
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2495..2515
                     /label=attB1
                     /note="Geneious type: recomb_site"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2527..2541
                     /label=Linker
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2542..2709
                     /label=VP64
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00011 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, pRJ_00011 
                     PCR Product, pRJ_00096, pRJ_00097"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2548..2580
                     /label=VP16min
                     /note="Geneious type: domain"
                     /note="/Transferred_From=VP64 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2587..2619
                     /label=VP16min
                     /note="Geneious type: domain"
                     /note="/Transferred_From=VP64 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2626..2658
                     /label=VP16min
                     /note="Geneious type: domain"
                     /note="/Transferred_From=VP64 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2665..2697
                     /label=VP16min
                     /note="Geneious type: domain"
                     /note="/Transferred_From=VP64 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2686..2709)
                     /label=RJ_00050Rc
                     /note="color: black; sequence: 
                     CAGATGGTCTCTCGTCTCTAGAGTTAATCAGCATGTCCAG"
     misc_feature    2710..2721
                     /label=Linker
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2722..2745
                     /label=SV40
                     /note="Geneious type: motif"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00096"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2746..3528
                     /label=p65
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00012 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00012, pRJ_00012 
                     PCR Product, pRJ_00096, pRJ_00098"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2791..2802
                     /label=NLS
                     /note="Geneious type: motif"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2797..2816
                     /label=FCS3
                     /note="color: black; sequence: AAGCGGACCTACGAGACATT"
     misc_feature    3133..3267
                     /label=Activation domain
                     /note="Geneious type: Region_of_interest"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3496..3522
                     /label=9aaTAD
                     /note="Geneious type: motif"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(3509..3529)
                     /label=RJ_00050Rd
                     /note="color: black; sequence: 
                     CAGATGGTCTCTCGTCCAGCAAGGCTGAGAAATCCA"
     misc_feature    3529..3546
                     /label=GS-rich Linker
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3547..4116
                     /label=Rta
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00013 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00013, pRJ_00013 
                     PCR Product, pRJ_00096, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     3555..3575
                     /label=FCS4
                     /note="color: black; sequence: CAGGGAAGGGATGTTTTTGCC"
     misc_feature    3634..3798
                     /label=Pro-rich
                     /note="Geneious type: Compositionally_biased"
                     /note="/Transferred_From=Rta (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(4094..4116)
                     /label=RJ_00050Rb
                     /note="color: black; sequence: 
                     CAGATGGTCTCTCGTCAAACAGAGATGTGTCGAAGATGG"
     misc_feature    4120..4147
                     /label=L0.3_Inert (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4120..4123
                     /label=pL0 G-3
                     /note="Geneious type: Gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4120..4123
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4120..4123
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    4120..4123
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4124..4143
                     /label=Inert
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4144..4200
                     /label=pBjh_4560 (concatenated sequence 7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4144..4147
                     /label=pL0 3-T
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4144..4147
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    4144..4147
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      4148..4196
                     /label=Synth Terminator (Short)
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0} width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/modified_by=User"
                     /note="color: #ffffff; direction: RIGHT"
     polyA_signal    4148..4153
                     /label=Poly-A Signal
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    complement(4159..4164)
                     /label=Poly-A Signal
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4197..6261
                     /label=L1.Seq1-Seq2_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4197..4200
                     /label=pL0 T
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4197..4200
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    4197..4200
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4201..4245
                     /label=seq2
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(4240..4245)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4292..4401
                     /label=RK2 trfa
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4406..4411
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      complement(4429..5111)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #ffff00"
     misc_feature    complement(4432..5051)
                     /label=pBR322 origin
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    5027..5032
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(5038..5043)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
       61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
      241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg ccagcgctgc
     1081 attaccctgt tatccctaac cggtttaccg agctcttatt ggttttcaaa cttcattgac
     1141 tgtgccggag ttctgcaatt catgtgatct gcgcctggga gctcacgggg acagcccccc
     1201 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     1261 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     1321 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     1381 tttgagcctg cagacacctg gggggatacg gggaaaaagc taggctgaaa gaggatctta
     1441 gaatgacacg cgcctgggag ctcacgggga cagccccccc ccaaagcccc cagggatgta
     1501 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     1561 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     1621 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     1681 ggggatacgg ggaaaaagct ggatcataac tcgacgtgca tactgtcccc tccaccccac
     1741 agtggggaca acttgtgccg tatcagtccc ctccacccca cagtggggaa tgccatcagt
     1801 cgtttggtcc cctccacccc acagtggggt tttaccctag tctacaagtc ccctccaccc
     1861 cacagtgggg cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
     1921 gaaccgtcag atcgcctgga gaattccaga taagtttgta caaaaaagag aggtgccacc
     1981 atgaagctac tgtcctctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
     2041 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
     2101 tctcccaaaa ccaaaaggtc cccgctgact agggcacatc tgacagaagt ggaatcaagg
     2161 ctagaaagac tggaacagct atttctactg atttttcctc gagaggacct tgacatgatt
     2221 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
     2281 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
     2341 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
     2401 caaagacagt tgactgtatc gatgggcccc aagaagaaga ggaaggtggg atctggctcg
     2461 ccagggatcc gtcgacttga cgcgttgata tcaacaagtt tgtacaaaaa agcaggctac
     2521 aaagaggcca gcggttccgg acgggctgac gcattggacg attttgatct ggatatgctg
     2581 ggaagtgacg ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac
     2641 tttgacctcg acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt
     2701 aactctagaa gttccggatc tccgaaaaag aaacgcaaag ttggtagcca gtacctgccc
     2761 gacaccgacg accggcaccg gatcgaggaa aagcggaagc ggacctacga gacattcaag
     2821 agcatcatga agaagtcccc cttcagcggc cccaccgacc ctagacctcc acctagaaga
     2881 atcgccgtgc ccagcagatc cagcgccagc gtgccaaaac ctgcccccca gccttacccc
     2941 ttcaccagca gcctgagcac catcaactac gacgagttcc ctaccatggt gttccccagc
     3001 ggccagatct ctcaggcctc tgctctggct ccagcccctc ctcaggtgct gcctcaggct
     3061 cctgctcctg caccagctcc agccatggtg tctgcactgg ctcaggcacc agcacccgtg
     3121 cctgtgctgg ctcctggacc tccacaggct gtggctccac cagcccctaa acctacacag
     3181 gccggcgagg gcacactgtc tgaagctctg ctgcagctgc agttcgacga cgaggatctg
     3241 ggagccctgc tgggaaacag caccgatcct gccgtgttca ccgacctggc cagcgtggac
     3301 aacagcgagt tccagcagct gctgaaccag ggcatccctg tggcccctca caccaccgag
     3361 cccatgctga tggaataccc cgaggccatc acccggctcg tgacaggcgc tcagaggcct
     3421 cctgatccag ctcctgcccc tctgggagca ccaggcctgc ctaatggact gctgtctggc
     3481 gacgaggact tcagctctat cgccgatatg gatttctcag ccttgctggg ctctggcagc
     3541 ggcagccggg attccaggga agggatgttt ttgccgaagc ctgaggccgg ctccgctatt
     3601 agtgacgtgt ttgagggccg cgaggtgtgc cagccaaaac gaatccggcc atttcatcct
     3661 ccaggaagtc catgggccaa ccgcccactc cccgccagcc tcgcaccaac accaaccggt
     3721 ccagtacatg agccagtcgg gtcactgacc ccggcaccag tccctcagcc actggatcca
     3781 gcgcccgcag tgactcccga ggccagtcac ctgttggagg atcccgatga agagacgagc
     3841 caggctgtca aagcccttcg ggagatggcc gatactgtga ttccccagaa ggaagaggct
     3901 gcaatctgtg gccaaatgga cctttcccat ccgcccccaa ggggccatct ggatgagctg
     3961 acaaccacac ttgagtccat gaccgaggat ctgaacctgg actcacccct gaccccggaa
     4021 ttgaacgaga ttctggatac cttcctgaac gacgagtgcc tcttgcatgc catgcatatc
     4081 agcacaggac tgtccatctt cgacacatct ctgttttgag cttccactgg attgtacaat
     4141 taccaacaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgcgct
     4201 ggtgcgtttt tatgcttgta gtattgtata atgtttttaa gatccttaat taatagggat
     4261 aacagggtaa tatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt
     4321 gggcctcggc ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt
     4381 cgccggcatc gttgcgccac atctaggatc tgccaggaac cgtaaaaagg ccgcgttgct
     4441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5101 tttggtcatg a
//
