LOCUS       pFC545                  3908 bp    DNA     circular SYN 14-AUG-2020
DEFINITION  cHS4-[gRNA1]miniCMV-Gal4-Hsf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(25..30)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(95..955)
                     /label=amp marker
                     /note="Geneious type: marker"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    98..955
                     /label=AmpR
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    793..798
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(810..815)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(997..1025)
                     /label=amp prom
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1102..1146
                     /label=seq1
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1724
                     /label=pBjh_3260 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=pL0 I
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1147..1150
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1151..1720
                     /label=2x cHS4 Insulator
                     /note="Geneious type: Insulator"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1229..1233
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    1508..1512
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    1721..1950
                     /label=PCR861 Correct (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=TACT
                     /note="Geneious type: protein_bind"
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1721..1724
                     /label=pL0 I-P
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1725..1744
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1745..1747
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1766..1785
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1786..1788
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1807..1826
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1827..1829
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1848..1867
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1868..1870
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     gene            1871..1946
                     /label=miniCMV
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1871..1933
                     /label=miniCMV
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1929
                     /label=Transcription starting point
                     /note="Geneious type: DNA probe bind"
                     /note="/Sequence=A"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1947..1974
                     /label=L0.5.2_Inert (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     unsure          1947..1950
                     /label=CAGA
                     /note="/created by=fabio"
                     /note="color: #a6acb3"
     misc_feature    1947..1950
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1947..1950
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     5'UTR           1951..1970
                     /label=Inert
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(1958..1980)
                     /label=FCS1
                     /note="color: black; sequence: GGTGGCACCTCTCTTTTTTGTAC"
     misc_feature    1971..2920
                     /label=pRJ_00126 (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..1974
                     /label=pL0 5-G
                     /note="Geneious type: Gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..1974
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1971..1974
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1975..1980
                     /label=Kozak
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1981..2916
                     /label=Gal4-NLS-HA-Hsf1
                     /note="/Transferred_From=(4) Digestion of pBjh_4071 - 
                     Fragment 1, Digestion of pBjh_4071 - Fragment 1, pBjh_4071"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1984..2421
                     /label=Gal4 DBD
                     /note="Geneious type: domain"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2011..2094
                     /label=Zn(2)-C6 fungal-type.{ECO:0000255|PROSITE-ProRu...
                     /note="Geneious type: dna_binding_domain"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2086..2104
                     /label=FCS2
                     /note="color: black; sequence: TGGGAGTGTCGCTACTCTC"
     primer_bind     2394..2420
                     /label=FCS6
                     /note="color: black; sequence: CAAAGGTCAAAGACAGTTGACTGTATC"
     misc_feature    2428..2448
                     /label=SV40 NLS
                     /note="Geneious type: motif"
                     /note="/ApEinfo_fwdcolor=#ff0000"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0} width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/Transferred_From=(4) Digestion of pBjh_4017 - 
                     Fragment 1, (4) Digestion of pBjh_4018 - Fragment 1, (4) 
                     Digestion of pBjh_4019 - Fragment 1, (4) Digestion of 
                     pBjh_4020 - Fragment 1, (4) Digestion of pBjh_4021 - 
                     Fragment 1, (4) Digestion of pBjh_4036 - Fragment 1, (4) 
                     Digestion of pRJ_00095 - Fragment 1, (4) Digestion of 
                     pRJ_00096 - Fragment 1, (4) Digestion of pRJ_00097 - 
                     Fragment 1, (4) Digestion of pRJ_00098 - Fragment 1, (4) 
                     Digestion of pRJ_00099 - Fragment 1, Digestion of pBjh_4017
                     - Fragment 1, Digestion of pBjh_4018 - Fragment 1, 
                     Digestion of pBjh_4019 - Fragment 1, Digestion of pBjh_4020
                     - Fragment 1, Digestion of pBjh_4021 - Fragment 1, 
                     Digestion of pBjh_4036 - Fragment 1, Digestion of pRJ_00095
                     - Fragment 1, Digestion of pRJ_00096 - Fragment 1, 
                     Digestion of pRJ_00097 - Fragment 1, Digestion of pRJ_00098
                     - Fragment 1, Digestion of pRJ_00099 - Fragment 1, 
                     pBjh_4017, pBjh_4018, pBjh_4019, pBjh_4020, pBjh_4021, 
                     pBjh_4036, pRJ_00095, pRJ_00096, pRJ_00097, pRJ_00098, 
                     pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=(4) Digestion of pBjh_4017 - 
                     Fragment1; (4) Digestion of pBjh_4018 - Fragment 1; (4) 
                     Digestionof pBjh_4019 - Fragment 1; (4) Digestion of 
                     pBjh_4020 -Fragment 1; (4) Digestion of pBjh_4021 - 
                     Fragment 1; (4)Digestion of pBjh_4036 - Fragment 1; 
                     Digestion ofpBjh_4017 - Fragment 1; Digestion of pBjh_4018 
                     - Fragment1; Digestion of pBjh_4019 - Fragment 1; Digestion
                     ofpBjh_4020 - Fragment 1; Digestion of pBjh_4021 - 
                     Fragment1; Digestion of pBjh_4036 - Fragment 1; 
                     pBjh_4017;pBjh_4018; pBjh_4019; pBjh_4020; pBjh_4021; 
                     pBjh_4036"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2428..2448
                     /label=RJ_00068Fa
                     /note="color: black; sequence: CCCAAGAAGAAGAGGAAGGTG"
     misc_feature    2449..2457
                     /label=GSG
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     sig_peptide     2458..2484
                     /label=HA-tag
                     /note="/Transferred_From=(4) Digestion of pRJ_00095 - 
                     Fragment 1, (4) Digestion of pRJ_00096 - Fragment 1, (4) 
                     Digestion of pRJ_00097 - Fragment 1, (4) Digestion of 
                     pRJ_00098 - Fragment 1, (4) Digestion of pRJ_00099 - 
                     Fragment 1, Digestion of pRJ_00095 - Fragment 1, Digestion 
                     of pRJ_00096 - Fragment 1, Digestion of pRJ_00097 - 
                     Fragment 1, Digestion of pRJ_00098 - Fragment 1, Digestion 
                     of pRJ_00099 - Fragment 1, pRJ_00095, pRJ_00096, pRJ_00097,
                     pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #cc99b2; direction: RIGHT"
     misc_feature    2485..2493
                     /label=GSG
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2493..2496
                     /label=Fuse to Gal4
                     /note="Geneious type: Gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2493..2496
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2497..2913
                     /label=Hsf1
                     /note="Geneious type: domain"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2536..2907
                     /label=Transactivation domain
                     /note="Geneious type: Region_of_interest"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2554..2580
                     /label=9aaTAD.
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2917..2944
                     /label=L0.3_Inert (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2917..2920
                     /label=pL0 G-3
                     /note="Geneious type: Gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2917..2920
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2917..2920
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    2917..2920
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2921..2940
                     /label=Inert
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2941..2997
                     /label=pBjh_4560 (concatenated sequence 7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2941..2944
                     /label=pL0 3-T
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2941..2944
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    2941..2944
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      2945..2993
                     /label=Synth Terminator (Short)
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0} width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/modified_by=User"
                     /note="color: #ffffff; direction: RIGHT"
     polyA_signal    2945..2950
                     /label=Poly-A Signal
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    complement(2956..2961)
                     /label=Poly-A Signal
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    2994..5058
                     /label=L1.Seq1-Seq2_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2994..2997
                     /label=pL0 T
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2994..2997
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    2994..2997
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2998..3042
                     /label=seq2
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(3037..3042)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    3089..3198
                     /label=RK2 trfa
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3203..3208
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      complement(3226..3908)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #ffff00"
     misc_feature    complement(3229..3848)
                     /label=pBR322 origin
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    3824..3829
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(3835..3840)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
       61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
      241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg ccagcgctgc
     1081 attaccctgt tatccctaac cggtttaccg agctcttatt ggttttcaaa cttcattgac
     1141 tgtgccggag ttctgcaatt catgtgatct gcgcctggga gctcacgggg acagcccccc
     1201 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     1261 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     1321 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     1381 tttgagcctg cagacacctg gggggatacg gggaaaaagc taggctgaaa gaggatctta
     1441 gaatgacacg cgcctgggag ctcacgggga cagccccccc ccaaagcccc cagggatgta
     1501 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     1561 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     1621 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     1681 ggggatacgg ggaaaaagct ggatcataac tcgacgtgca tactgtcccc tccaccccac
     1741 agtggggaca acttgtgccg tatcagtccc ctccacccca cagtggggaa tgccatcagt
     1801 cgtttggtcc cctccacccc acagtggggt tttaccctag tctacaagtc ccctccaccc
     1861 cacagtgggg cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
     1921 gaaccgtcag atcgcctgga gaattccaga taagtttgta caaaaaagag aggtgccacc
     1981 atgaagctac tgtcctctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
     2041 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
     2101 tctcccaaaa ccaaaaggtc cccgctgact agggcacatc tgacagaagt ggaatcaagg
     2161 ctagaaagac tggaacagct atttctactg atttttcctc gagaggacct tgacatgatt
     2221 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
     2281 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
     2341 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
     2401 caaagacagt tgactgtatc gatgggcccc aagaagaaga ggaaggtggg atctggttac
     2461 ccatacgacg tgccagatta cgccggatca ggttgctcac agatttcctc tagtgggcag
     2521 ggaggaggtg gaagcggctt cagcgtggac accagtgccc tgctggacct gttcagcccc
     2581 tcggtgaccg tgcccgacat gagcctgcct gaccttgaca gcagcctggc cagtatccaa
     2641 gagctcctgt ctccccagga gccccccagg cctcccgagg cagagaacag cagcccggat
     2701 tcagggaagc agctggtgca ctacacagcg cagccgctgt tcctgctgga ccccggctcc
     2761 gtggacaccg ggagcaacga cctgccggtg ctgtttgagc tgggagaggg ctcctacttc
     2821 tccgaagggg acggcttcgc cgaggacccc accatctccc tgctgacagg ctcggagcct
     2881 cccaaagcca aggaccccac tgtctccggt agttgagctt ccactggatt gtacaattac
     2941 caacaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgcgctggt
     3001 gcgtttttat gcttgtagta ttgtataatg tttttaagat ccttaattaa tagggataac
     3061 agggtaatat cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg
     3121 cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc
     3181 cggcatcgtt gcgccacatc taggatctgc caggaaccgt aaaaaggccg cgttgctggc
     3241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3661 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3901 ggtcatga
//
