LOCUS       pFC546                  4373 bp    DNA     circular SYN 14-AUG-2020
DEFINITION  cHS4-[gRNA1]miniCMV-Gal4-YY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(25..30)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(95..955)
                     /label=amp marker
                     /note="Geneious type: marker"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    98..955
                     /label=AmpR
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    793..798
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(810..815)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(997..1025)
                     /label=amp prom
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1102..1146
                     /label=seq1
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1724
                     /label=pBjh_3260 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=pL0 I
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1147..1150
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1151..1720
                     /label=2x cHS4 Insulator
                     /note="Geneious type: Insulator"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1229..1233
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    1508..1512
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    1721..1950
                     /label=PCR861 Correct (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=pL0 I-P
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1721..1724
                     /label=TACT
                     /note="Geneious type: protein_bind"
                     /note="/Transferred_From=pBjh_4574"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1725..1744
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1745..1747
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1766..1785
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1786..1788
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1807..1826
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1827..1829
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     misc_feature    1848..1867
                     /label=gRNA BS
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1868..1870
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3"
     gene            1871..1946
                     /label=miniCMV
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1871..1933
                     /label=miniCMV
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1929
                     /label=Transcription starting point
                     /note="Geneious type: DNA probe bind"
                     /note="/Sequence=A"
                     /note="/created by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1947..1974
                     /label=L0.5.2_Inert (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1947..1950
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     unsure          1947..1950
                     /label=CAGA
                     /note="/created by=fabio"
                     /note="color: #a6acb3"
     misc_feature    1947..1950
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     5'UTR           1951..1970
                     /label=Inert
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..3385
                     /label=pRJ_00127 (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..1974
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    1971..1974
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1971..1974
                     /label=pL0 5-G
                     /note="Geneious type: Gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1975..1980
                     /label=Kozak
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1981..3381
                     /label=Gal4-NLS-HA-YY1
                     /note="/Transferred_From=(4) Digestion of pBjh_4071 - 
                     Fragment 1, Digestion of pBjh_4071 - Fragment 1, pBjh_4071"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    1984..2421
                     /label=Gal4 DBD
                     /note="Geneious type: domain"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2011..2094
                     /label=Zn(2)-C6 fungal-type.{ECO:0000255|PROSITE-ProRu...
                     /note="Geneious type: dna_binding_domain"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2428..2448
                     /label=SV40 NLS
                     /note="Geneious type: motif"
                     /note="/ApEinfo_fwdcolor=#ff0000"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0} width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/Transferred_From=(4) Digestion of pBjh_4017 - 
                     Fragment 1, (4) Digestion of pBjh_4018 - Fragment 1, (4) 
                     Digestion of pBjh_4019 - Fragment 1, (4) Digestion of 
                     pBjh_4020 - Fragment 1, (4) Digestion of pBjh_4021 - 
                     Fragment 1, (4) Digestion of pBjh_4036 - Fragment 1, (4) 
                     Digestion of pRJ_00095 - Fragment 1, (4) Digestion of 
                     pRJ_00096 - Fragment 1, (4) Digestion of pRJ_00097 - 
                     Fragment 1, (4) Digestion of pRJ_00098 - Fragment 1, (4) 
                     Digestion of pRJ_00099 - Fragment 1, Digestion of pBjh_4017
                     - Fragment 1, Digestion of pBjh_4018 - Fragment 1, 
                     Digestion of pBjh_4019 - Fragment 1, Digestion of pBjh_4020
                     - Fragment 1, Digestion of pBjh_4021 - Fragment 1, 
                     Digestion of pBjh_4036 - Fragment 1, Digestion of pRJ_00095
                     - Fragment 1, Digestion of pRJ_00096 - Fragment 1, 
                     Digestion of pRJ_00097 - Fragment 1, Digestion of pRJ_00098
                     - Fragment 1, Digestion of pRJ_00099 - Fragment 1, 
                     pBjh_4017, pBjh_4018, pBjh_4019, pBjh_4020, pBjh_4021, 
                     pBjh_4036, pRJ_00095, pRJ_00096, pRJ_00097, pRJ_00098, 
                     pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/X-Transferred_From=(4) Digestion of pBjh_4017 - 
                     Fragment1; (4) Digestion of pBjh_4018 - Fragment 1; (4) 
                     Digestionof pBjh_4019 - Fragment 1; (4) Digestion of 
                     pBjh_4020 -Fragment 1; (4) Digestion of pBjh_4021 - 
                     Fragment 1; (4)Digestion of pBjh_4036 - Fragment 1; 
                     Digestion ofpBjh_4017 - Fragment 1; Digestion of pBjh_4018 
                     - Fragment1; Digestion of pBjh_4019 - Fragment 1; Digestion
                     ofpBjh_4020 - Fragment 1; Digestion of pBjh_4021 - 
                     Fragment1; Digestion of pBjh_4036 - Fragment 1; 
                     pBjh_4017;pBjh_4018; pBjh_4019; pBjh_4020; pBjh_4021; 
                     pBjh_4036"
                     /note="/X-Transferred_Similarity=100.00%"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     2428..2448
                     /label=RJ_00068Fa
                     /note="color: black; sequence: CCCAAGAAGAAGAGGAAGGTG"
     misc_feature    2449..2457
                     /label=GSG
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     sig_peptide     2458..2484
                     /label=HA-tag
                     /note="/Transferred_From=(4) Digestion of pRJ_00095 - 
                     Fragment 1, (4) Digestion of pRJ_00096 - Fragment 1, (4) 
                     Digestion of pRJ_00097 - Fragment 1, (4) Digestion of 
                     pRJ_00098 - Fragment 1, (4) Digestion of pRJ_00099 - 
                     Fragment 1, Digestion of pRJ_00095 - Fragment 1, Digestion 
                     of pRJ_00096 - Fragment 1, Digestion of pRJ_00097 - 
                     Fragment 1, Digestion of pRJ_00098 - Fragment 1, Digestion 
                     of pRJ_00099 - Fragment 1, pRJ_00095, pRJ_00096, pRJ_00097,
                     pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #cc99b2; direction: RIGHT"
     primer_bind     2484..2504
                     /label=RJ_00226Fa
                     /note="color: black; sequence: AACTTCGGATCAGGTTGCATGGCGTC"
     misc_feature    2485..2493
                     /label=GSG
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2493..2496
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2493..2496
                     /label=Fuse to Gal4
                     /note="Geneious type: Gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2497..3378
                     /label=YY1
                     /note="Geneious type: domain"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2497..3006
                     /label=Interaction with the SMAD1/SMAD4 complex.
                     /note="Geneious type: Region_of_interest"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2623..2655
                     /label=Asp/Glu-rich (acidic).
                     /note="Geneious type: Compositionally_biased"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2656..2703
                     /label=Gly-Rich
                     /note="Geneious type: Compositionally_biased"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2704..2736
                     /label=Poly-His
                     /note="Geneious type: Compositionally_biased"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2971..3006
                     /label=Gly/Ser-Rich
                     /note="Geneious type: Compositionally_biased"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3265..3378
                     /label=Involved in nuclear matrix association.
                     /note="Geneious type: Region_of_interest"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3382..3409
                     /label=L0.3_Inert (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3382..3385
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3382..3385
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    3382..3385
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3382..3385
                     /label=pL0 G-3
                     /note="Geneious type: Gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3386..3405
                     /label=Inert
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3406..3462
                     /label=pBjh_4560 (concatenated sequence 7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3406..3409
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    3406..3409
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3406..3409
                     /label=pL0 3-T
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      3410..3458
                     /label=Synth Terminator (Short)
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0} width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/modified_by=User"
                     /note="color: #ffffff; direction: RIGHT"
     polyA_signal    3410..3415
                     /label=Poly-A Signal
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    complement(3421..3426)
                     /label=Poly-A Signal
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    3459..5523
                     /label=L1.Seq1-Seq2_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3459..3462
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3"
     misc_feature    3459..3462
                     /label=pL0 T
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3459..3462
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3463..3507
                     /label=seq2
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(3502..3507)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    3554..3663
                     /label=RK2 trfa
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3668..3673
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      complement(3691..4373)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #ffff00"
     misc_feature    complement(3694..4313)
                     /label=pBR322 origin
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4289..4294
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(4300..4305)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
       61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
      241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg ccagcgctgc
     1081 attaccctgt tatccctaac cggtttaccg agctcttatt ggttttcaaa cttcattgac
     1141 tgtgccggag ttctgcaatt catgtgatct gcgcctggga gctcacgggg acagcccccc
     1201 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     1261 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     1321 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     1381 tttgagcctg cagacacctg gggggatacg gggaaaaagc taggctgaaa gaggatctta
     1441 gaatgacacg cgcctgggag ctcacgggga cagccccccc ccaaagcccc cagggatgta
     1501 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     1561 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     1621 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     1681 ggggatacgg ggaaaaagct ggatcataac tcgacgtgca tactgtcccc tccaccccac
     1741 agtggggaca acttgtgccg tatcagtccc ctccacccca cagtggggaa tgccatcagt
     1801 cgtttggtcc cctccacccc acagtggggt tttaccctag tctacaagtc ccctccaccc
     1861 cacagtgggg cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
     1921 gaaccgtcag atcgcctgga gaattccaga taagtttgta caaaaaagag aggtgccacc
     1981 atgaagctac tgtcctctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
     2041 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
     2101 tctcccaaaa ccaaaaggtc cccgctgact agggcacatc tgacagaagt ggaatcaagg
     2161 ctagaaagac tggaacagct atttctactg atttttcctc gagaggacct tgacatgatt
     2221 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
     2281 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
     2341 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
     2401 caaagacagt tgactgtatc gatgggcccc aagaagaaga ggaaggtggg atctggttac
     2461 ccatacgacg tgccagatta cgccggatca ggttgcatgg cgtctggcga cacattgtat
     2521 atagctacag atggaagcga aatgcctgct gaaatcgtag aactgcatga gatcgaggtg
     2581 gaaaccatcc ccgtggagac aatcgaaaca actgtagtgg gggaagaaga agaagaggat
     2641 gacgatgatg aggatggagg gggaggtgat catggaggtg gaggtggtca cggccacgcc
     2701 gggcaccatc atcatcatca ccaccaccac catcatcccc cgatgatcgc cctgcagcca
     2761 ctcgtaacag atgatcctac gcaagtacat caccatcagg aagtaatact tgtgcagacg
     2821 agggaagagg tcgtgggcgg cgacgactca gatggtcttc gcgcggaaga tggtttcgag
     2881 gatcaaattc tcatccccgt tcccgcgccc gccggcggtg acgacgatta tatcgaacaa
     2941 acccttgtga ccgtggctgc ggctggcaag agtggcggtg gtggctcaag cagttccggt
     3001 ggaggcagag tcaaaaaagg cggaggaaaa aagtcaggca aaaagagtta cctctccggg
     3061 ggagctggtg cggccggcgg aggcggagca gatccaggta ataagaaatg ggaacagaag
     3121 caggtccaaa tcaaaaccct ggagggagag ttctcagtta ctatgtggag ctccgacgag
     3181 aagaaggaca tagaccatga aaccgttgtc gaggagcaaa taatcggtga gaatagcccg
     3241 cccgactata gcgagtatat gacaggaaaa aaactcccgc ccggcggcat ccccggcatc
     3301 gacctttctg atcccaaaca acttgctgaa tttgcaagaa tgaagccccg gaagataaag
     3361 gaagatgatg caccacgctg agcttccact ggattgtaca attaccaaca ataaaatatc
     3421 tttattttca ttacatctgt gtgttggttt tttgtgtgcg ctggtgcgtt tttatgcttg
     3481 tagtattgta taatgttttt aagatcctta attaataggg ataacagggt aatatcttgc
     3541 cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc
     3601 caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc
     3661 acatctagga tctgccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tga
//
