LOCUS       pEV745                  5195 bp    DNA     circular SYN 29-OCT-2020
DEFINITION  cHS4-2xgRNA1_BS-YB_TATA-Kozak2-Gal4-VPR.
ACCESSION   urn.local...1si-cjz3hzz
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
COMMENT     Imported From
            Path: /Users/elviravitu/Dropbox (MIT)/Elvira 
            collaboration/Plasmids/Resheaper library
            Filename: 10 documents from Reshpaer first cloning round.gb
FEATURES             Location/Qualifiers
     source          1..5195
                     /lab_host="."
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1791..1823
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          4047..4280
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(25..30)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(95..955)
                     /label=amp marker
                     /note="Geneious type: marker"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    98..955
                     /label=AmpR
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    793..798
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(810..815)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(997..1025)
                     /label=amp prom
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1102..1146
                     /label=seq1
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1724
                     /label=pBjh_3260 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=pL0 I
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1150
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1151..1720
                     /label=2x cHS4 Insulator
                     /note="Geneious type: Insulator"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1229..1233
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    1508..1512
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    1721..1827
                     /label=pEV700 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=pL0 I-P
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1721..1724
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1721..1724
                     /label=TACT
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     rRNA            1725..1744
                     /label=gRNA1 BS
                     /note="/created_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1745..1747
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     rRNA            1766..1785
                     /label=gRNA1 BS
                     /note="/created_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1786..1788
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1789..1823
                     /label=Promoter
                     /note="Geneious type: motif"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1799..1823
                     /label=YB_TATA
                     /note="/created_by=elviravitu"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1824..1870
                     /label=pEV711 (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     1824..1866
                     /label=EV1041-F
                     /note="color: black; sequence: 
                     AATGCTAGTCTACTACCAGAGCTCATCGCTAGCGCTACCGGTC"
     misc_feature    1824..1827
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1824..1827
                     /label=AATG
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(1828..1870)
                     /label=EV1041-R
                     /note="color: black; sequence: 
                     ACCTGACCGGTAGCGCTAGCGATGAGCTCTGGTAGTAGACTAG"
     unsure          1828..1866
                     /label=5' UTR
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3"
     misc_feature    1867..4022
                     /label=pEV739 (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1867..2326
                     /label=pEV719 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1867..1870
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1867..1870
                     /label=AGGT
                     /note="/created_by=elviravitu"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    1867..1870
                     /label=AGGT
                     /note="Geneious type: Q"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1867..1870
                     /label=Ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1871..1880
                     /label=Kozak2
                     /note="Geneious type: motif"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1877..1900
                     /label=Gal4-NLS-VPR
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     CDS             1901..2326
                     /label=Gal4
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2323..4022
                     /label=pEV731 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2323..4018
                     /codon_start=2
                     /label=Gal4-NLS-VPR
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #993366"
                     /translation="GPQEEEEGGIWLARDPST*RVDINKFVQKSRLQRGQRFRTG*RIG
                     RF*SGYAGK*RPR*F*P*HAWFGCP**L*PRHARQ*RP**FRPGHAD*L*KFRISEKET
                     QSW*PVPARHRRPAPDRGKAEADLRDIQEHHEEVPLQRPHRP*TST*KNRRAQQIQRQR
                     AKTCPPALPLHQQPEHHQLRRVPYHGVPQRPDLSGLCSGSSPSSGAASGSCSCTSSSHG
                     VCTGSGTSTRACAGSWTSTGCGSTSP*TYTGRRGHTV*SSAAAAVRRRGSGSPAGKQHR
                     SCRVHRPGQRGQQRVPAAAEPGHPCGPSHHRAHADGIPRGHHPARDRRSEAS*SSSCPS
                     GSTRPA*WTAVWRRGLQLYRRYGFLSLAGLWQRQPGFQGRDVFAEA*GRLRY**RV*GP
                     RGVPAKTNPAISSSRKSMGQPPTPRQPRTNTNRSST*ASRVTDPGTSPSATGSSARSDS
                     RGQSPVGGSR*RDEPGCQSPSGDGRYCDSPEGRGCNLWPNGPFPSAPKGPSG*ADNHT*
                     VHDRGSEPGLTPDPGIERDSGYLPERRVPLACHAYQHRTVHLRHISVL"
     misc_feature    2323..2326
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2323..2326
                     /label=GGGC
                     /note="Geneious type: Q"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2327..2347
                     /label=SV40 NLS
                     /note="Geneious type: motif"
                     /note="/Transferred_From=Digestion of pRJ_00009 PCR Product
                     - Fragment 2, Digestion of pRJ_00010 PCR Product - Fragment
                     2, Digestion of pRJ_00011 PCR Product - Fragment 2, 
                     Digestion of pRJ_00012 PCR Product - Fragment 2, Digestion 
                     of pRJ_00013 PCR Product - Fragment 2, pRJ_00009, pRJ_00009
                     PCR Product, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, 
                     pRJ_00011 PCR Product, pRJ_00012, pRJ_00012 PCR Product, 
                     pRJ_00013, pRJ_00013 PCR Product, pRJ_00095, pRJ_00096, 
                     pRJ_00097, pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2348..2356
                     /label=GSG
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2394..2414
                     /label=attB1
                     /note="Geneious type: recomb_site"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2441..2608
                     /label=VP64
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00011 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, pRJ_00011 
                     PCR Product, pRJ_00096, pRJ_00097"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2621..2644
                     /label=SV40
                     /note="Geneious type: motif"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00096"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2645..3427
                     /label=p65
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00012 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00012, pRJ_00012 
                     PCR Product, pRJ_00096, pRJ_00098"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2690..2701
                     /label=NLS
                     /note="Geneious type: motif"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3032..3166
                     /label=Activation domain
                     /note="Geneious type: Region_of_interest"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3428..3445
                     /label=GS-rich Linker
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3446..4015
                     /label=Rta
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00013 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00013, pRJ_00013 
                     PCR Product, pRJ_00096, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    join(4019..4022,1867..1870)
                     /label=L0.G_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/NCBI Join Type=join"
                     /note="/Source"
                     /note="This forward directional feature has 2 segments:
                        1: 4019 .. 4022 / #a6acb3
                        2: 1867 .. 1870 / #a6acb3"
     misc_feature    4019..4046
                     /label=pEV542 (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4019..4022
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4019..4022
                     /label=GCTT
                     /note="Geneious type: Q"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     unsure          4019..4022
                     /label=Ligation
                     /note="/created_by=fabio"
                     /note="color: #a6acb3"
     misc_feature    4043..4284
                     /label=pEV618 (concatenated sequence 7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4043..4046
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    4043..4046
                     /label=CAAC
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     polyA_signal    4047..4280
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     regulatory      4169..4170
                     /label=CS
                     /note="/created_by=elviravitu"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      4196..4197
                     /label=bGH2
                     /note="/created_by=elviravitu"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      4222..4223
                     /label=bGH6
                     /note="/created_by=elviravitu"
                     /note="/modified_by=elviravitu"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      complement(4253..4254)
                     /label=2
                     /note="/created_by=elviravitu"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    4281..6345
                     /label=L1.Seq1-Seq2_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4281..4284
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    4281..4284
                     /label=CGCT
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    4281..4284
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4285..4329
                     /label=seq2
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(4324..4329)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4376..4485
                     /label=RK2 trfa
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4490..4495
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      complement(4513..5195)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #ffff00"
     misc_feature    complement(4516..5135)
                     /label=pBR322 origin
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    5111..5116
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(5122..5127)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
       61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
      241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg ccagcgctgc
     1081 attaccctgt tatccctaac cggtttaccg agctcttatt ggttttcaaa cttcattgac
     1141 tgtgccggag ttctgcaatt catgtgatct gcgcctggga gctcacgggg acagcccccc
     1201 cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc
     1261 ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga
     1321 cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc
     1381 tttgagcctg cagacacctg gggggatacg gggaaaaagc taggctgaaa gaggatctta
     1441 gaatgacacg cgcctgggag ctcacgggga cagccccccc ccaaagcccc cagggatgta
     1501 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     1561 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     1621 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     1681 ggggatacgg ggaaaaagct ggatcataac tcgacgtgca tactgtcccc tccaccccac
     1741 agtggggcat agcgccggcc gcgccgtccc ctccacccca cagtggggca ccatatgctc
     1801 tagagggtat ataatggggg ccaaatgcta gtctactacc agagctcatc gctagcgcta
     1861 ccggtcaggt gcccttatgg tgaagctact gtcctctatc gaacaagcat gcgatatttg
     1921 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
     1981 caactgggag tgtcgctact ctcccaaaac caaaaggtcc ccgctgacta gggcacatct
     2041 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
     2101 agaggacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
     2161 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
     2221 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
     2281 agagagtagt aacaaaggtc aaagacagtt gactgtatcg atgggcccca agaagaagag
     2341 gaaggtggga tctggctcgc cagggatccg tcgacttgac gcgttgatat caacaagttt
     2401 gtacaaaaaa gcaggctaca aagaggccag cggttccgga cgggctgacg cattggacga
     2461 ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg acatgcttgg
     2521 ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc ttgatgattt
     2581 cgacctggac atgctgatta actctagaag ttccggatct ccgaaaaaga aacgcaaagt
     2641 tggtagccag tacctgcccg acaccgacga ccggcaccgg atcgaggaaa agcggaagcg
     2701 gacctacgag acattcaaga gcatcatgaa gaagtccccc ttcagcggcc ccaccgaccc
     2761 tagacctcca cctagaagaa tcgccgtgcc cagcagatcc agcgccagcg tgccaaaacc
     2821 tgccccccag ccttacccct tcaccagcag cctgagcacc atcaactacg acgagttccc
     2881 taccatggtg ttccccagcg gccagatctc tcaggcctct gctctggctc cagcccctcc
     2941 tcaggtgctg cctcaggctc ctgctcctgc accagctcca gccatggtgt ctgcactggc
     3001 tcaggcacca gcacccgtgc ctgtgctggc tcctggacct ccacaggctg tggctccacc
     3061 agcccctaaa cctacacagg ccggcgaggg cacactgtct gaagctctgc tgcagctgca
     3121 gttcgacgac gaggatctgg gagccctgct gggaaacagc accgatcctg ccgtgttcac
     3181 cgacctggcc agcgtggaca acagcgagtt ccagcagctg ctgaaccagg gcatccctgt
     3241 ggcccctcac accaccgagc ccatgctgat ggaatacccc gaggccatca cccggctcgt
     3301 gacaggcgct cagaggcctc ctgatccagc tcctgcccct ctgggagcac caggcctgcc
     3361 taatggactg ctgtctggcg acgaggactt cagctctatc gccgatatgg atttctcagc
     3421 cttgctgggc tctggcagcg gcagccggga ttccagggaa gggatgtttt tgccgaagcc
     3481 tgaggccggc tccgctatta gtgacgtgtt tgagggccgc gaggtgtgcc agccaaaacg
     3541 aatccggcca tttcatcctc caggaagtcc atgggccaac cgcccactcc ccgccagcct
     3601 cgcaccaaca ccaaccggtc cagtacatga gccagtcggg tcactgaccc cggcaccagt
     3661 ccctcagcca ctggatccag cgcccgcagt gactcccgag gccagtcacc tgttggagga
     3721 tcccgatgaa gagacgagcc aggctgtcaa agcccttcgg gagatggccg atactgtgat
     3781 tccccagaag gaagaggctg caatctgtgg ccaaatggac ctttcccatc cgcccccaag
     3841 gggccatctg gatgagctga caaccacact tgagtccatg accgaggatc tgaacctgga
     3901 ctcacccctg accccggaat tgaacgagat tctggatacc ttcctgaacg acgagtgcct
     3961 cttgcatgcc atgcatatca gcacaggact gtccatcttc gacacatctc tgttttgagc
     4021 ttccactgga ttgtacaatt accaaccagc ctcgactgtg ccttctagtt gccagccatc
     4081 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4141 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4201 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4261 ggatgcggtg ggctctatgg cgctggtgcg tttttatgct tgtagtattg tataatgttt
     4321 ttaagatcct taattaatag ggataacagg gtaatatctt gccctgcacg aataccagcg
     4381 accccttgcc caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga
     4441 agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gatctgccag
     4501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     4981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5041 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5101 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5161 gaacgaaaac tcacgttaag ggattttggt catga
//
