LOCUS       pEV823                  5209 bp    DNA     circular SYN 27-JAN-2025
DEFINITION  [S1-2] cHS4-2xgRNA1_BS-YB_TATA-1.5xuORF-K3-Gal4-VPR-polya(SV40).
ACCESSION   urn.local...1sj-cjz3hzz
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
COMMENT     Imported From
            Path: /Users/elviravitu/Dropbox (MIT)/Elvira 
            collaboration/Plasmids/Resheaper library
            Filename: 10 documents from Reshpaer first cloning round.gb
FEATURES             Location/Qualifiers
     source          1..5209
                     /lab_host="."
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1802..1834
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(25..30)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    98..955
                     /label=AmpR
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    793..798
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(810..815)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(997..1025)
                     /label=amp prom
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1102..1146
                     /label=seq1
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1735
                     /label=pBjh_3260 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1161
                     /label=pL0 I
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1161
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1161
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1161
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1162..1731
                     /label=2x cHS4 Insulator
                     /note="Geneious type: Insulator"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1240..1244
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    1519..1523
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    1732..1838
                     /label=pEV700 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     rRNA            1736..1755
                     /label=gRNA1 BS
                     /note="/created_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1756..1758
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     rRNA            1777..1796
                     /label=gRNA1 BS
                     /note="/created_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1797..1799
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1800..1834
                     /label=Promoter
                     /note="Geneious type: motif"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1810..1834
                     /label=YB_TATA
                     /note="/created_by=elviravitu"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1835..1915
                     /label=pEV711 (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     unsure          1839..1877
                     /label=5' UTR
                     /note="/created by=elviravitu"
                     /note="color: #a6acb3"
     misc_feature    1880..1891
                     /label=uORF x1 weak
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1880..1890
                     /label=Digestion of 13.56 - Fragment 2 extraction (con...
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1895..1911
                     /label=Digestion of 13.58 - Fragment 2 extraction (con...
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1901..1912
                     /label=uORF x1
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1916..4067
                     /label=pEV740 (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1916..2371
                     /label=pEV720 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1916..1925
                     /label=Kozak3
                     /note="Geneious type: motif"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1922..1945
                     /label=Gal4-NLS-VPR
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     CDS             1946..2371
                     /label=Gal4
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2368..4067
                     /label=pEV731 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2368..4063
                     /codon_start=2
                     /label=Gal4-NLS-VPR
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #993366"
                     /translation="GPQEEEEGGIWLARDPST*RVDINKFVQKSRLQRGQRFRTG*RIG
                     RF*SGYAGK*RPR*F*P*HAWFGCP**L*PRHARQ*RP**FRPGHAD*L*KFRISEKET
                     QSW*PVPARHRRPAPDRGKAEADLRDIQEHHEEVPLQRPHRP*TST*KNRRAQQIQRQR
                     AKTCPPALPLHQQPEHHQLRRVPYHGVPQRPDLSGLCSGSSPSSGAASGSCSCTSSSHG
                     VCTGSGTSTRACAGSWTSTGCGSTSP*TYTGRRGHTV*SSAAAAVRRRGSGSPAGKQHR
                     SCRVHRPGQRGQQRVPAAAEPGHPCGPSHHRAHADGIPRGHHPARDRRSEAS*SSSCPS
                     GSTRPA*WTAVWRRGLQLYRRYGFLSLAGLWQRQPGFQGRDVFAEA*GRLRY**RV*GP
                     RGVPAKTNPAISSSRKSMGQPPTPRQPRTNTNRSST*ASRVTDPGTSPSATGSSARSDS
                     RGQSPVGGSR*RDEPGCQSPSGDGRYCDSPEGRGCNLWPNGPFPSAPKGPSG*ADNHT*
                     VHDRGSEPGLTPDPGIERDSGYLPERRVPLACHAYQHRTVHLRHISVL"
     misc_feature    2368..2371
                     /label=GGGC
                     /note="Geneious type: Q"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2368..2371
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2372..2392
                     /label=SV40 NLS
                     /note="Geneious type: motif"
                     /note="/Transferred_From=Digestion of pRJ_00009 PCR Product
                     - Fragment 2, Digestion of pRJ_00010 PCR Product - Fragment
                     2, Digestion of pRJ_00011 PCR Product - Fragment 2, 
                     Digestion of pRJ_00012 PCR Product - Fragment 2, Digestion 
                     of pRJ_00013 PCR Product - Fragment 2, pRJ_00009, pRJ_00009
                     PCR Product, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, 
                     pRJ_00011 PCR Product, pRJ_00012, pRJ_00012 PCR Product, 
                     pRJ_00013, pRJ_00013 PCR Product, pRJ_00095, pRJ_00096, 
                     pRJ_00097, pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2393..2401
                     /label=GSG
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2439..2459
                     /label=attB1
                     /note="Geneious type: recomb_site"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2486..2653
                     /label=VP64
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00011 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, pRJ_00011 
                     PCR Product, pRJ_00096, pRJ_00097"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2666..2689
                     /label=SV40
                     /note="Geneious type: motif"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00096"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2690..3472
                     /label=p65
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00012 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00012, pRJ_00012 
                     PCR Product, pRJ_00096, pRJ_00098"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2735..2746
                     /label=NLS
                     /note="Geneious type: motif"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3077..3211
                     /label=Activation domain
                     /note="Geneious type: Region_of_interest"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3473..3490
                     /label=GS-rich Linker
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3491..4060
                     /label=Rta
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00013 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00013, pRJ_00013 
                     PCR Product, pRJ_00096, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4064..4091
                     /label=pEV542 (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     unsure          4064..4067
                     /label=Ligation
                     /note="/created_by=fabio"
                     /note="color: #a6acb3"
     misc_feature    4064..4067
                     /label=L0.G_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/NCBI Join Type=join"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4088..4287
                     /label=pEV618 (concatenated sequence 7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    4092..4283
                     /label=SV40 polyA
                     /note="/created by=User"
                     /note="/modified by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4284..6359
                     /label=L1.Seq1-Seq2_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4299..4343
                     /label=seq2
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(4338..4343)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4390..4499
                     /label=RK2 trfa
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4504..4509
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      complement(4527..5209)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #ffff00"
     misc_feature    5125..5130
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(5136..5141)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
       61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
      241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      961 tcctttttca atattattga agcatttatc aaggttattg tctcatgagc ggatacatat
     1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg ccagcgctgc
     1081 attaccctgt tatccctaac cggtttaccg agctcttatt ggttttcaaa cttcattgac
     1141 tgtgccgctc ttcaaccgga gttctgcaat tcatgtgatc tgcgcctggg agctcacggg
     1201 gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag
     1261 cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca
     1321 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct
     1381 tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag ctaggctgaa
     1441 agaggatctt agaatgacac gcgcctggga gctcacgggg acagcccccc cccaaagccc
     1501 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     1561 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     1621 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     1681 cagacacctg gggggatacg gggaaaaagc tggatcataa ctcgacgtgc atactgtccc
     1741 ctccacccca cagtggggca tagcgccggc cgcgccgtcc cctccacccc acagtggggc
     1801 accatatgct ctagagggta tataatgggg gccaaatgct agtctactac cagagctcat
     1861 cgctagcgct accggtcctc ttatgggttg atcgctcgca accatgggtt gaggtgcctt
     1921 tatggtgaag ctactgtcct ctatcgaaca agcatgcgat atttgccgac ttaaaaagct
     1981 caagtgctcc aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg
     2041 ctactctccc aaaaccaaaa ggtccccgct gactagggca catctgacag aagtggaatc
     2101 aaggctagaa agactggaac agctatttct actgattttt cctcgagagg accttgacat
     2161 gattttgaaa atggattctt tacaggatat aaaagcattg ttaacaggat tatttgtaca
     2221 agataatgtg aataaagatg ccgtcacaga tagattggct tcagtggaga ctgatatgcc
     2281 tctaacattg agacagcata gaataagtgc gacatcatca tcggaagaga gtagtaacaa
     2341 aggtcaaaga cagttgactg tatcgatggg ccccaagaag aagaggaagg tgggatctgg
     2401 ctcgccaggg atccgtcgac ttgacgcgtt gatatcaaca agtttgtaca aaaaagcagg
     2461 ctacaaagag gccagcggtt ccggacgggc tgacgcattg gacgattttg atctggatat
     2521 gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg atgcccttga
     2581 tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc tggacatgct
     2641 gattaactct agaagttccg gatctccgaa aaagaaacgc aaagttggta gccagtacct
     2701 gcccgacacc gacgaccggc accggatcga ggaaaagcgg aagcggacct acgagacatt
     2761 caagagcatc atgaagaagt cccccttcag cggccccacc gaccctagac ctccacctag
     2821 aagaatcgcc gtgcccagca gatccagcgc cagcgtgcca aaacctgccc cccagcctta
     2881 ccccttcacc agcagcctga gcaccatcaa ctacgacgag ttccctacca tggtgttccc
     2941 cagcggccag atctctcagg cctctgctct ggctccagcc cctcctcagg tgctgcctca
     3001 ggctcctgct cctgcaccag ctccagccat ggtgtctgca ctggctcagg caccagcacc
     3061 cgtgcctgtg ctggctcctg gacctccaca ggctgtggct ccaccagccc ctaaacctac
     3121 acaggccggc gagggcacac tgtctgaagc tctgctgcag ctgcagttcg acgacgagga
     3181 tctgggagcc ctgctgggaa acagcaccga tcctgccgtg ttcaccgacc tggccagcgt
     3241 ggacaacagc gagttccagc agctgctgaa ccagggcatc cctgtggccc ctcacaccac
     3301 cgagcccatg ctgatggaat accccgaggc catcacccgg ctcgtgacag gcgctcagag
     3361 gcctcctgat ccagctcctg cccctctggg agcaccaggc ctgcctaatg gactgctgtc
     3421 tggcgacgag gacttcagct ctatcgccga tatggatttc tcagccttgc tgggctctgg
     3481 cagcggcagc cgggattcca gggaagggat gtttttgccg aagcctgagg ccggctccgc
     3541 tattagtgac gtgtttgagg gccgcgaggt gtgccagcca aaacgaatcc ggccatttca
     3601 tcctccagga agtccatggg ccaaccgccc actccccgcc agcctcgcac caacaccaac
     3661 cggtccagta catgagccag tcgggtcact gaccccggca ccagtccctc agccactgga
     3721 tccagcgccc gcagtgactc ccgaggccag tcacctgttg gaggatcccg atgaagagac
     3781 gagccaggct gtcaaagccc ttcgggagat ggccgatact gtgattcccc agaaggaaga
     3841 ggctgcaatc tgtggccaaa tggacctttc ccatccgccc ccaaggggcc atctggatga
     3901 gctgacaacc acacttgagt ccatgaccga ggatctgaac ctggactcac ccctgacccc
     3961 ggaattgaac gagattctgg ataccttcct gaacgacgag tgcctcttgc atgccatgca
     4021 tatcagcaca ggactgtcca tcttcgacac atctctgttt tgagcttcca ctggattgta
     4081 caattaccaa ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     4141 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     4201 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     4261 gggggaggtg tgggaggttt tttcgctcgc tgaagagcgg tgcgttttta tgcttgtagt
     4321 attgtataat gtttttaaga tccttaatta atagggataa cagggtaata tcttgccctg
     4381 cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa
     4441 acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat
     4501 ctaggatctg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4561 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4621 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4681 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4741 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4801 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4861 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4921 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4981 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5041 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5101 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5161 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatga
//
