LOCUS       pEV824                  5208 bp    DNA     circular SYN 27-JAN-2025
DEFINITION  [S1-2] cHS4-2xgRNA1_BS-YB_TATA-2xuORF-K3-Gal4-VPR-polya(SV40).
ACCESSION   urn.local...1sj-cjz3hzz
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
COMMENT     Imported From
            Path: /Users/elviravitu/Dropbox (MIT)/Elvira 
            collaboration/Plasmids/Resheaper library
            Filename: 10 documents from Reshpaer first cloning round.gb
FEATURES             Location/Qualifiers
     source          1..5208
                     /lab_host="."
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1802..1834
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(25..30)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    98..955
                     /label=AmpR
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    793..798
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(810..815)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(997..1025)
                     /label=amp prom
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    1102..1146
                     /label=seq1
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1735
                     /label=pBjh_3260 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1161
                     /label=pL0 I
                     /note="Geneious type: gg_overhang"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1161
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1161
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1147..1161
                     /label=ligation
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1162..1731
                     /label=2x cHS4 Insulator
                     /note="Geneious type: Insulator"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    1240..1244
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     protein_bind    1519..1523
                     /label=CTCF Bind (Short)
                     /note="/created_by=User"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    1732..1838
                     /label=pEV700 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     rRNA            1736..1755
                     /label=gRNA1 BS
                     /note="/created_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1756..1758
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     rRNA            1777..1796
                     /label=gRNA1 BS
                     /note="/created_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    1797..1799
                     /label=PAM
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1800..1834
                     /label=Promoter
                     /note="Geneious type: motif"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        1810..1834
                     /label=YB_TATA
                     /note="/created_by=elviravitu"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    1835..1914
                     /label=pEV711 (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     unsure          1839..1877
                     /label=5' UTR
                     /note="/created by=elviravitu"
                     /note="color: #a6acb3"
     misc_feature    1879..1890
                     /label=uORF x1
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1879..1889
                     /label=Digestion of 13.58 - Fragment 2 extraction (con...
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1894..1910
                     /label=Digestion of 13.58 - Fragment 2 extraction (con...
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1900..1911
                     /label=uORF x1
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1915..4066
                     /label=pEV740 (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1915..2370
                     /label=pEV720 (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    1915..1924
                     /label=Kozak3
                     /note="Geneious type: motif"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             1921..1944
                     /label=Gal4-NLS-VPR
                     /note="/created_by=User"
                     /note="/modified by=elviravitu"
                     /note="/modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     CDS             1945..2370
                     /label=Gal4
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    2367..4066
                     /label=pEV731 (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             2367..4062
                     /codon_start=2
                     /label=Gal4-NLS-VPR
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="/modified_by=elviravitu"
                     /note="color: #993366"
                     /translation="GPQEEEEGGIWLARDPST*RVDINKFVQKSRLQRGQRFRTG*RIG
                     RF*SGYAGK*RPR*F*P*HAWFGCP**L*PRHARQ*RP**FRPGHAD*L*KFRISEKET
                     QSW*PVPARHRRPAPDRGKAEADLRDIQEHHEEVPLQRPHRP*TST*KNRRAQQIQRQR
                     AKTCPPALPLHQQPEHHQLRRVPYHGVPQRPDLSGLCSGSSPSSGAASGSCSCTSSSHG
                     VCTGSGTSTRACAGSWTSTGCGSTSP*TYTGRRGHTV*SSAAAAVRRRGSGSPAGKQHR
                     SCRVHRPGQRGQQRVPAAAEPGHPCGPSHHRAHADGIPRGHHPARDRRSEAS*SSSCPS
                     GSTRPA*WTAVWRRGLQLYRRYGFLSLAGLWQRQPGFQGRDVFAEA*GRLRY**RV*GP
                     RGVPAKTNPAISSSRKSMGQPPTPRQPRTNTNRSST*ASRVTDPGTSPSATGSSARSDS
                     RGQSPVGGSR*RDEPGCQSPSGDGRYCDSPEGRGCNLWPNGPFPSAPKGPSG*ADNHT*
                     VHDRGSEPGLTPDPGIERDSGYLPERRVPLACHAYQHRTVHLRHISVL"
     misc_feature    2367..2370
                     /label=GGGC
                     /note="Geneious type: Q"
                     /note="/created_by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2367..2370
                     /label=Ligation
                     /note="Geneious type: ligation"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2371..2391
                     /label=SV40 NLS
                     /note="Geneious type: motif"
                     /note="/Transferred_From=Digestion of pRJ_00009 PCR Product
                     - Fragment 2, Digestion of pRJ_00010 PCR Product - Fragment
                     2, Digestion of pRJ_00011 PCR Product - Fragment 2, 
                     Digestion of pRJ_00012 PCR Product - Fragment 2, Digestion 
                     of pRJ_00013 PCR Product - Fragment 2, pRJ_00009, pRJ_00009
                     PCR Product, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, 
                     pRJ_00011 PCR Product, pRJ_00012, pRJ_00012 PCR Product, 
                     pRJ_00013, pRJ_00013 PCR Product, pRJ_00095, pRJ_00096, 
                     pRJ_00097, pRJ_00098, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2392..2400
                     /label=GSG
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2438..2458
                     /label=attB1
                     /note="Geneious type: recomb_site"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2485..2652
                     /label=VP64
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00011 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00011, pRJ_00011 
                     PCR Product, pRJ_00096, pRJ_00097"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2665..2688
                     /label=SV40
                     /note="Geneious type: motif"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00096"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2689..3471
                     /label=p65
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00012 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00012, pRJ_00012 
                     PCR Product, pRJ_00096, pRJ_00098"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    2734..2745
                     /label=NLS
                     /note="Geneious type: motif"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3076..3210
                     /label=Activation domain
                     /note="Geneious type: Region_of_interest"
                     /note="/Transferred_From=p65 (pBjh_4067)"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3472..3489
                     /label=GS-rich Linker
                     /note="Geneious type: motif"
                     /note="/created_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    3490..4059
                     /label=Rta
                     /note="Geneious type: domain"
                     /note="/Transferred_From=Digestion of pRJ_00010 PCR Product
                     - Fragment 2, Digestion of pRJ_00013 PCR Product - Fragment
                     2, pRJ_00010, pRJ_00010 PCR Product, pRJ_00013, pRJ_00013 
                     PCR Product, pRJ_00096, pRJ_00099"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4063..4090
                     /label=pEV542 (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     unsure          4063..4066
                     /label=Ligation
                     /note="/created_by=fabio"
                     /note="color: #a6acb3"
     misc_feature    4063..4066
                     /label=L0.G_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/NCBI Join Type=join"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4087..4286
                     /label=pEV618 (concatenated sequence 7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    4091..4282
                     /label=SV40 polyA
                     /note="/created by=User"
                     /note="/modified by=elviravitu"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4283..6358
                     /label=L1.Seq1-Seq2_Backbone (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4298..4342
                     /label=seq2
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(4337..4342)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4389..4498
                     /label=RK2 trfa
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4503..4508
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     rep_origin      complement(4526..5208)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #ffff00"
     misc_feature    5124..5129
                     /label=BBb scar
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    complement(5135..5140)
                     /label=BBb scar
                     /note="color: #a6acb3; direction: LEFT"
ORIGIN
        1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
       61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
      241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      961 tcctttttca atattattga agcatttatc aaggttattg tctcatgagc ggatacatat
     1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg ccagcgctgc
     1081 attaccctgt tatccctaac cggtttaccg agctcttatt ggttttcaaa cttcattgac
     1141 tgtgccgctc ttcaaccgga gttctgcaat tcatgtgatc tgcgcctggg agctcacggg
     1201 gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag
     1261 cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca
     1321 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct
     1381 tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag ctaggctgaa
     1441 agaggatctt agaatgacac gcgcctggga gctcacgggg acagcccccc cccaaagccc
     1501 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     1561 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     1621 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     1681 cagacacctg gggggatacg gggaaaaagc tggatcataa ctcgacgtgc atactgtccc
     1741 ctccacccca cagtggggca tagcgccggc cgcgccgtcc cctccacccc acagtggggc
     1801 accatatgct ctagagggta tataatgggg gccaaatgct agtctactac cagagctcat
     1861 cgctagcgct accggtctac catgggttga tcgctcgcaa ccatgggttg aggtgccttt
     1921 atggtgaagc tactgtcctc tatcgaacaa gcatgcgata tttgccgact taaaaagctc
     1981 aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc
     2041 tactctccca aaaccaaaag gtccccgctg actagggcac atctgacaga agtggaatca
     2101 aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagagga ccttgacatg
     2161 attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt atttgtacaa
     2221 gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac tgatatgcct
     2281 ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag tagtaacaaa
     2341 ggtcaaagac agttgactgt atcgatgggc cccaagaaga agaggaaggt gggatctggc
     2401 tcgccaggga tccgtcgact tgacgcgttg atatcaacaa gtttgtacaa aaaagcaggc
     2461 tacaaagagg ccagcggttc cggacgggct gacgcattgg acgattttga tctggatatg
     2521 ctgggaagtg acgccctcga tgattttgac cttgacatgc ttggttcgga tgcccttgat
     2581 gactttgacc tcgacatgct cggcagtgac gcccttgatg atttcgacct ggacatgctg
     2641 attaactcta gaagttccgg atctccgaaa aagaaacgca aagttggtag ccagtacctg
     2701 cccgacaccg acgaccggca ccggatcgag gaaaagcgga agcggaccta cgagacattc
     2761 aagagcatca tgaagaagtc ccccttcagc ggccccaccg accctagacc tccacctaga
     2821 agaatcgccg tgcccagcag atccagcgcc agcgtgccaa aacctgcccc ccagccttac
     2881 cccttcacca gcagcctgag caccatcaac tacgacgagt tccctaccat ggtgttcccc
     2941 agcggccaga tctctcaggc ctctgctctg gctccagccc ctcctcaggt gctgcctcag
     3001 gctcctgctc ctgcaccagc tccagccatg gtgtctgcac tggctcaggc accagcaccc
     3061 gtgcctgtgc tggctcctgg acctccacag gctgtggctc caccagcccc taaacctaca
     3121 caggccggcg agggcacact gtctgaagct ctgctgcagc tgcagttcga cgacgaggat
     3181 ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt tcaccgacct ggccagcgtg
     3241 gacaacagcg agttccagca gctgctgaac cagggcatcc ctgtggcccc tcacaccacc
     3301 gagcccatgc tgatggaata ccccgaggcc atcacccggc tcgtgacagg cgctcagagg
     3361 cctcctgatc cagctcctgc ccctctggga gcaccaggcc tgcctaatgg actgctgtct
     3421 ggcgacgagg acttcagctc tatcgccgat atggatttct cagccttgct gggctctggc
     3481 agcggcagcc gggattccag ggaagggatg tttttgccga agcctgaggc cggctccgct
     3541 attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa aacgaatccg gccatttcat
     3601 cctccaggaa gtccatgggc caaccgccca ctccccgcca gcctcgcacc aacaccaacc
     3661 ggtccagtac atgagccagt cgggtcactg accccggcac cagtccctca gccactggat
     3721 ccagcgcccg cagtgactcc cgaggccagt cacctgttgg aggatcccga tgaagagacg
     3781 agccaggctg tcaaagccct tcgggagatg gccgatactg tgattcccca gaaggaagag
     3841 gctgcaatct gtggccaaat ggacctttcc catccgcccc caaggggcca tctggatgag
     3901 ctgacaacca cacttgagtc catgaccgag gatctgaacc tggactcacc cctgaccccg
     3961 gaattgaacg agattctgga taccttcctg aacgacgagt gcctcttgca tgccatgcat
     4021 atcagcacag gactgtccat cttcgacaca tctctgtttt gagcttccac tggattgtac
     4081 aattaccaac cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     4141 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     4201 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     4261 ggggaggtgt gggaggtttt ttcgctcgct gaagagcggt gcgtttttat gcttgtagta
     4321 ttgtataatg tttttaagat ccttaattaa tagggataac agggtaatat cttgccctgc
     4381 acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa
     4441 cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc
     4501 taggatctgc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4981 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatga
//
