LOCUS       pSRG101_after_se        4928 bp DNA     circular SYN 04-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2025 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(460..528)
                     /label=LacZ alpha
     primer_bind     599..616
                     /label=M13-fwd
     primer_bind     622..642
                     /label=T7
     misc_feature    695..700
                     /locus_tag="KasI"
                     /label=KasI
     intron          740..792
                     /locus_tag="smu-1 introns"
                     /label=smu-1 introns
     primer_bind     829..850
                     /label=oES_045 GCN for MED-1 F
     primer_bind     830..847
                     /label=oES_043 GCN4 F
     primer_bind     complement(832..850)
                     
     misc_feature    832..846
                     /locus_tag="New Feature"
                     /label=New Feature
     misc_feature    847..2574
                     /locus_tag="24xGCN4_v4"
                     /label=24xGCN4_v4
     misc_feature    847..918
                     /label=GCN4 nr 1
     misc_feature    904..918
                     /label=GSGSG linker
     misc_feature    919..990
                     /label=GCN4 nr 2
     misc_feature    991..1062
                     /label=GCN4 nr 3
     misc_feature    1063..1134
                     /label=GCN4 nr 4
     misc_feature    1135..1206
                     /label=GCN4 nr 5
     misc_feature    1207..1278
                     /label=GCN4 nr 6
     misc_feature    1279..1350
                     /label=GCN4 nr 7
     misc_feature    1351..1422
                     /label=GCN4 nr 8
     misc_feature    1423..1494
                     /label=GCN4 nr 9
     misc_feature    1495..1566
                     /label=GCN4 nr 10
     primer_bind     complement(1539..1558)
                     /label=oES_084 GCN4 nr10 R
     misc_feature    1567..1638
                     /label=GCN4 nr 11
     misc_feature    1639..1710
                     /label=GCN4 nr 12
     misc_feature    1711..1782
                     /label=GCN4 nr 13
     misc_feature    1783..1854
                     /label=GCN4 nr 14
     misc_feature    1855..1926
                     /label=GCN4 nr 15
     misc_feature    1927..1998
                     /label=GCN4 nr 16
     misc_feature    1999..2070
                     /label=GCN4 nr 17
     misc_feature    2071..2142
                     /label=GCN4 nr 18
     misc_feature    2143..2214
                     /label=GCN4 nr 19
     misc_feature    2215..2286
                     /label=GCN4 nr 20
     misc_feature    2287..2358
                     /label=GCN4 nr 21
     misc_feature    2359..2430
                     /label=GCN4 nr 22
     misc_feature    2431..2502
                     /label=GCN4 nr 23
     misc_feature    2503..2574
                     /label=GCN4 nr 24
     primer_bind     2560..2593
                     /label=spES_001
     primer_bind     2572..2589
                     /label=oSR_22 22_Gblock_3tagBFP_H2B F
     misc_feature    2585..2590
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    2591..2596
                     /locus_tag="Acc65I"
                     /label=Acc65I
     misc_feature    2608..2613
                     /locus_tag="SnaBI"
                     /label=SnaBI
     misc_feature    2614..2667
                     /locus_tag="T2A"
                     /label=T2A
     primer_bind     2668..2678
                     /label=oES_053 F cloning pES_016
     primer_bind     complement(2738..2757)
                     /label=T3
     primer_bind     complement(2775..2795)
                     /label=M13-rev
     misc_binding    complement(2801..2823)
                     /label=LacO
     rep_origin      complement(3163..3845)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(3943..4602)
                     /label=AmpR
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc
      661 ccccctcgag gtcgacggta tcgataagct tatgggcgcc atcatacacg cggccgcaat
      721 catcggtccg gatatcaagg tattttcctg catttttcaa ctgggaaaat gaaagaaaat
      781 cgataatttc aggaagatat caggcttatc ggtccgggtg gatctggagg tggaggttct
      841 ggaggagaag aacttttgag caagaattat catcttgaga acgaagtggc tcgtcttaag
      901 aaaggttctg gcagtggaga agaactgctt tcaaagaatt accacctgga aaatgaggta
      961 gctagactga aaaaggggag cggaagtggg gaggagttgc tgagcaaaaa ttatcatttg
     1021 gagaacgaag tagcacgact aaagaaaggg tccggatcgg gtgaggagtt actctcgaaa
     1081 aattatcatc tcgaaaacga agtggctcgg ctaaaaaagg gcagtggttc tggagaagag
     1141 ctattatcta aaaactacca cctcgaaaat gaggtggcac gcttaaaaaa gggaagtggc
     1201 agtggtgaag agctactatc caagaattat catcttgaga acgaggtagc gcgtttgaag
     1261 aagggttccg gctcaggaga ggaactgctc tcgaagaact atcatcttga aaatgaggtc
     1321 gctcgattaa aaaagggatc gggcagtggt gaggaactac tttcaaagaa ttaccacctc
     1381 gaaaacgaag tagctcgatt aaagaaaggt tcagggtcgg gtgaagaatt actgagtaaa
     1441 aattatcatc tggaaaatga ggtagcgaga ctaaaaaagg ggagtggttc tggcgaagag
     1501 ttgctatcga aaaattatca tcttgagaac gaagttgcta ggctcaaaaa gggctcaggc
     1561 tcaggcgagg agttgctctc gaaaaactac cacttggaaa atgaggtcgc gaggttgaaa
     1621 aaggggagcg ggtcgggcga ggagttattg agcaaaaact atcatttaga gaacgaagtc
     1681 gcgcgcttaa agaaaggctc gggctcgggc gaagaactct tatcgaagaa ctaccacctc
     1741 gaaaatgagg tcgccaggtt gaaaaagggc agtggcagcg gggaggaact cttgagcaag
     1801 aactaccact tggagaatga ggtcgcgaga ttgaagaaag ggtcggggag cggcgaggaa
     1861 ttgctcagca agaattatca tttggagaac gaagtcgcca ggctcaagaa aggctcgggg
     1921 tcgggggagg aattgttgag taaaaactac cacttggaaa atgaagtcgc caggctcaaa
     1981 aaagggagtg ggagcggcga agagttattg agcaaaaatt accacttgga gaacgaagtg
     2041 gcaaggctca agaaagggag cggcagcggg gaggagctct tatcgaagaa ctaccactta
     2101 gagaatgaag tcgcccgctt gaagaaaggc tcggggagcg gggaagagct cttgagcaag
     2161 aactaccact tggaaaatga ggtggcgcgc ttgaagaaag ggagcgggag cggggaagag
     2221 ttactatcta agaattatca tctcgagaac gaggtggctc gactaaagaa gggctccggc
     2281 agtggggagg aactcctgtc gaagaactat catcttgaaa atgaggttgc aagacttaaa
     2341 aaggggtccg gatcaggtga ggaactactc agtaagaatt accacctgga aaacgaagtt
     2401 gcacgtttga agaaaggatc aggatcaggc gaagaactgc tctcaaaaga ttatcatttg
     2461 gaaaatgagg ttgcacgttt aaaaaaggga agtggcagtg gtgaggaact tctgtcgaaa
     2521 aattatcatc tcgagaatga agtagcccga cttaaaaagg gttctggctc gggtcagcgg
     2581 ccgcaccggt ggtaccagga agctagctac gtagagggac gtggatccct cctcacctgc
     2641 ggagacgtcg aggagaaccc aggaccacct aggctgcagc ccgggggatc cactagttct
     2701 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaatttc
     2761 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2821 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2881 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2941 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3001 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3601 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3901 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3961 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4021 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4081 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4141 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4201 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4261 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4321 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4381 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4441 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4501 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4561 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4621 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4681 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4741 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4801 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4861 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4921 agtgccac
//
