LOCUS       pTW335l_-_[colE1]_Ptac_ 7513 bp ds-DNA     circular     20-MAR-2025
DEFINITION  .
ACCESSION   urn.local...16e-aqel875
SOURCE      null
COMMENT     Imported using the Genbank importer. File name: pTW004c.gb [ColE1]
            Ptet T7RNAP N-term aadA. Source name: pTW002 - AP SC101 RepA Ptet
            SD8 MBP bla tetR (Note: Geneious sometimes stores the sequence name
            in the source attribute) Source name: pTW002a - CP ColEI Rop
            dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious sometimes stores
            the sequence name in the source attribute) Source name: pTW002a -
            CP ColEI Rop dnak-P1 SD8 LuxABtetA-orfL aadA (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW002 - AP SC101 RepA Ptet SD8 MBP bla tetR (Note: Geneious
            sometimes stores the sequence name in the source attribute) Source
            name: pTW001b - AP SC101 RepA Ptet SD8 MBP(wt) bla (Note: Geneious
            sometimes stores the sequence name in the source attribute)
FEATURES             Location/Qualifiers
     misc_feature    455..597
                     /label="color: #a6acb3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(699..890)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(2202..3284)
                     /label="lacI"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     promoter        complement(3285..3362)
                     /label="lacI promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     promoter        3605..3633
                     /label="tac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     operator        3637..3659
                     /label="lacO"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     RBS             3725..3737
                     /label="RBS"
                     /ApEinfo_revcolor="#82ca9d"
                     /ApEinfo_fwdcolor="#82ca9d"
     misc_feature    3738..5180
                     /label="GhRbcL"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             3738..5180
                     /label="Translation 3738-5180"
                     /translation="MSPQTETKASVGFKAGVKEYKLTYYTPEYEVKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYDIEPVPGEEDQYICYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRVPTAYIKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEAIFKSQAETGEIKGHYLNATAGTCEEMIKRAMCARELGVPIVMHDYLTGGFTANTSLAHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRMSGGDHIHAGTVVGKLEGERDITLGFVDLLRDDFIEKDRSRGIYFTQDWVSMPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAREGNEIIREASKWSPELAAACEVWKAIKFEFDAVDKLDKVEK*"
     RBS             5193..5215
                     /label="RBS"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     CDS             5223..5246
                     /label="Translation 5223-5246"
                     /translation="MCMQVWPP"
     misc_feature    5223..5618
                     /label="GhRbcS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             5247..5618
                     /label="Translation 5247-5618"
                     /translation="LGKKKFETLSYLPDLTPVQLAKEVDYLLRSKWIPCLEFELEEGFVHRKYSSLPTYYDGRYWTMWKLPMFGCTDSAQVLEELENCKKEYPNAFIRIIGFDNVRQVQCISFIAYKPKGYHHHHHH*"
     misc_feature    5598..5615
                     /label="6xHis"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_fwdcolor="#bc8dbf"
     terminator      5695..5742
                     /label="T7 terminator (pET)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        5968..6162
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     CDS             6361..7020
                     /label="AmpR"
                     /ApEinfo_revcolor="#7bcdc8"
                     /ApEinfo_fwdcolor="#7bcdc8"
     rep_origin      7118..287
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     origin          7178..284
                     /label="pBR322"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
       61 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      421 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
      481 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
      601 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      661 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      721 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
      781 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
      841 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
      901 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
      961 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     1021 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     1081 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     1141 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     1201 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     1261 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     1321 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
     1381 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     1441 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     1501 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     1561 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     1621 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag
     1681 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     1741 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     1801 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     1861 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     1921 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     1981 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     2041 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     2101 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     2161 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     2221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2281 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     2341 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     2401 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
     2461 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
     2521 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     2581 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     2641 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     2701 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     2761 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
     2821 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     2881 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     2941 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3001 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     3061 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     3121 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     3181 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     3241 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     3301 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     3361 tcgcgcaacg caattaatgt aagttagctc actcattagg cacaattctc atgtttgaca
     3421 gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     3481 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
     3541 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
     3601 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
     3661 cacacaggaa acagccagtc cgtttaggtg ttttcacgag cacttcacca acaatAGGTG
     3721 CAGTaaggag atataccatg tcaccacaaa cagagactaa agcatccgtc ggttttaaag
     3781 ccggggtaaa agaatataaa ttgacctact ataccccaga atacgaggtc aaggatactg
     3841 acattttggc ggcgttccgc gtgactcctc aacccggcgt tccgccggag gaagcgggcg
     3901 cagcggtggc cgcggaatca tctaccggaa cgtggacaac tgtgtggacc gatggcctga
     3961 ccagcctgga tcgctataaa ggccgttgtt acgatattga gccggtgccg ggtgaagaag
     4021 atcaatacat ttgctacgta gcgtacccgt tagatctgtt tgaagaaggc agcgtcacca
     4081 atatgttcac aagtatcgtt ggcaatgtgt ttggttttaa ggccctgcgt gcgctacgat
     4141 tagaggacct gcgtgtgcct acggcctata tcaagacctt tcagggaccc ccgcatggta
     4201 ttcaggtgga acgtgataaa ctgaacaaat acggccggcc gcttctggga tgcaccatta
     4261 aaccaaagct gggcttaagt gcgaaaaatt atgggcgcgc cgtttatgag tgcctgcgtg
     4321 gaggtttgga tttcaccaaa gatgatgaaa acgtaaactc gcagccgttc atgcgatggc
     4381 gcgataggtt tctgttctgt gccgaagcta ttttcaaatc gcaagcagaa accggagaaa
     4441 tcaaaggtca ctatttaaat gcaaccgcag ggacctgtga agagatgatc aagcgcgcca
     4501 tgtgcgcgcg tgaactgggc gttcctatcg taatgcatga ctatctgaca ggtggtttca
     4561 cggcaaacac gagcctggcc cactactgcc gcgacaatgg cttactttta cacattcata
     4621 gagcaatgca cgcggtcatt gatcgccaga aaaatcacgg catgcatttt cgcgtgcttg
     4681 ccaaggcgct gcgcatgagc ggcggggatc acatacatgc agggacagta gtaggcaaac
     4741 tagagggcga gcgtgacatt acgctcggct tcgttgatct tctccgcgat gatttcatcg
     4801 aaaaagatcg ctctcgcggt atttatttta cccaggactg ggtgtcaatg ccgggtgttc
     4861 tccctgtggc tagcggcggc attcatgtgt ggcatatgcc ggcgctgacg gaaatctttg
     4921 gcgacgattc agttctgcag tttggtgggg gtaccctggg ccatccgtgg ggcaacgctc
     4981 ccggcgcggt ggctaaccgt gtggctctcg aggcgtgcgt ccaggcacgc aacgaaggcc
     5041 gtgacttggc ccgggaggga aatgaaatca ttcgtgaagc ctccaaatgg agcccggaac
     5101 tagctgctgc gtgtgaggtc tggaaagcaa ttaagtttga atttgacgcc gtggataaac
     5161 ttgacaaagt tgaaaaataa gctAGAAATA ATTTTGTtta actttaagaa ggagatatac
     5221 atatgtgtat gcaagtttgg cccccccttg gtaaaaaaaa gtttgaaacc ctgtcatatt
     5281 tgccggatct gacacctgtg caattagcga aggaagtcga ttacctactg cgtagcaaat
     5341 ggattccctg cctggagttc gaactcgaag aaggatttgt gcatcgtaaa tactccagct
     5401 taccgacgta ttatgatggc cgctactgga ccatgtggaa actgcctatg tttggctgca
     5461 ctgactcggc acaagtgctt gaggagctgg aaaactgtaa aaaggaatat ccgaatgcct
     5521 tcattcgaat tattggtttc gacaacgttc gccaggtaca gtgcatcagt tttatcgcgt
     5581 ataaaccaaa aggctaccat caccaccatc atcattaaAT CCGGCTGCTa acaaagcccg
     5641 aaaggaagct gagtggctgc tgccaccgct gagcaataaa ccgctgagca ataactagca
     5701 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     5761 tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
     5821 ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct
     5881 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct
     5941 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     6001 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     6061 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6121 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6181 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6241 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6301 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6361 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     6421 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6481 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6541 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6601 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6661 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     6721 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6781 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6841 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6901 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6961 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7021 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7081 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7201 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7261 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7321 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7381 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7441 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7501 cggtcgggct gaa
//