LOCUS       pBL155_-_[ColE1]_cCadC- 4296 bp ds-DNA     circular     20-MAR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(428..619)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     USER junction   716..724
                     /label="USER021"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     USER junction   718..729
                     /label="USER006"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      730..774
                     /label="rrnB1 short"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     USER junction   782..796
                     /label="USER008"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein bind... 816..834
                     /label="tetR"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     promoter        835..870
                     /label="Ptet"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     protein bind... 870..888
                     /label="tetR"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     misc_feature    921..935
                     /label="USER004_BL"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     USER junction   927..935
                     /label="USER009"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             936..949
                     /label="sd8"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             950..1429
                     /label="cadC 1-155 Leu16 TM GCN4 ZIP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             950..1429
                     /label="Translation 950-1429"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLRPRLIDLLVFFAQHSGEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMVPVIWYSEEEGEEIMLSSAAAISEAVPATDSPSHSLNIQNTATPPEQSPVKSKRGGSGG"
     gene            953..1414
                     /label="cadC 1-155"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1031..1039
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1037..1039
                     /label="E30R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1307..1321
                     /label="AAAIS mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1309..1317
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     USER junction   1420..1429
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             1430..2866
                     /label="AtRbcL"
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_fwdcolor="#6ecff6"
     CDS             1430..2866
                     /label="Translation 1430-2866"
                     /translation="APQTETKASVGFKAGVKEYKLTYYTPEYETKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYHIEPVPGEETQFIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALAALRLEDLRIPPAYTKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEAIYKSQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLSHYCRDNGLLLHIHRAMHAVIDRQKNHGMHFRVLAKALRLSGGDHIHAGTVVGKLEGDRESTLGFVDLLRDDYVEKDRSRGIFFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQARNEGRDLAVEGNEIIREACKWSPELAAACEVWKEITFNFPTIDKLDGQE*"
     USER junction   2867..2878
                     /label="USER002"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      complement(2886..2921)
                     /label="p14/tonB bidirectional terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     terminator      complement(2888..2919)
                     /label="tonB"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     USER junction   2922..2934
                     /label="USER005"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     cloning         2922..2934
                     /label="fragment"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(2935..3594)
                     /label="cat"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     USER junction   complement(2964..2971)
                     /label="USER028"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(3595..3692)
                     /label="cat promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     misc_feature    3698..3708
                     /label="USER030"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     origin          3709..4296
                     /label="colE1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
      661 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacaagagg
      721 acatccggtc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttgctgag
      781 gagacttagg gaccctccat cgaatggcca gatgatccct atcagtgata gagattgaca
      841 ctctatcatt gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga
      901 atagttcgac aaaaatctag acctgcAGGT GCAGTaaagg aaaaaaaaaa tgcaacaacc
      961 tgtagttcgc gttggcgaat ggcttgttac tccgtccata aaccaaatta gccgcaatgg
     1021 gcgtcaactt acccttCGTc cgagattaat cgatcttctg gttttctttg ctcaacacag
     1081 tggcgaagta cttagcaggg atgaacttat cgataatgtc tggaagagaa gtattgtcac
     1141 caatcacgtt gtgacgcaga gtatctcaga actacgtaag tcattaaaag ataatgatga
     1201 agatagtcct gtctatatcg ctactgtacc aaagcgcggc tataaattaa tggtgccggt
     1261 tatctggtac agcgaagaag agggagagga aataatgcta tcttcgGCAG CGGCTATCTC
     1321 Tgaggcggtt cctgccacag attctccctc ccacagtctt aacattcaaa acaccgcaac
     1381 gccacctgaa caatccccag ttaaaagcaa acgaggtggA TCTGGTGGTG CACCACAAAC
     1441 AGAGACTAAA GCAAGTGTTG GGTTCAAAGC TGGTGTTAAA GAGTATAAAT TGACTTACTA
     1501 TACTCCTGAA TATGAAACCA AGGATACTGA TATCTTGGCA GCATTCCGAG TAACTCCTCA
     1561 ACCTGGAGTT CCACCTGAAG AAGCAGGGGC TGCGGTAGCT GCTGAATCTT CTACTGGTAC
     1621 ATGGACAACT GTGTGGACCG ATGGGCTTAC CAGCCTTGAT CGTTACAAAG GACGATGCTA
     1681 CCACATCGAG CCCGTTCCAG GAGAAGAAAC TCAATTTATT GCGTATGTAG CTTATCCCTT
     1741 AGACCTTTTT GAAGAAGGTT CGGTTACTAA CATGTTTACC TCGATTGTGG GTAATGTATT
     1801 TGGGTTCAAA GCCCTGGCTG CTCTACGTCT AGAGGATCTG CGAATCCCTC CTGCTTATAC
     1861 TAAAACTTTC CAAGGACCAC CTCATGGTAT CCAAGTTGAA AGAGATAAAT TGAACAAGTA
     1921 TGGACGTCCC CTATTAGGAT GTACTATTAA ACCAAAATTG GGGTTATCCG CGAAAAACTA
     1981 TGGTAGAGCA GTTTATGAAT GTCTACGTGG TGGACTTGAT TTTACCAAAG ATGATGAGAA
     2041 TGTGAACTCC CAACCATTTA TGCGTTGGAG AGACCGTTTC TTATTTTGTG CCGAAGCTAT
     2101 TTATAAATCA CAGGCTGAAA CAGGTGAAAT CAAAGGGCAT TATTTGAATG CTACTGCGGG
     2161 TACATGCGAA GAAATGATCA AAAGAGCTGT ATTTGCCAGA GAATTGGGAG TTCCTATCGT
     2221 AATGCATGAC TACTTAACAG GGGGATTCAC CGCAAATACT AGTTTGTCTC ATTATTGCCG
     2281 AGATAATGGC CTACTTCTTC ACATCCACCG TGCAATGCAC GCTGTTATTG ATAGACAGAA
     2341 GAATCATGGT ATGCACTTCC GTGTACTAGC TAAAGCTTTA CGTCTATCTG GTGGAGATCA
     2401 TATTCACGCG GGTACAGTAG TAGGTAAACT TGAAGGAGAC AGGGAGTCAA CTTTGGGCTT
     2461 TGTTGATTTA CTGCGCGATG ATTATGTTGA AAAAGATCGA AGCCGCGGTA TCTTTTTCAC
     2521 TCAAGATTGG GTCTCACTAC CTGGTGTTCT GCCTGTGGCT TCAGGGGGTA TTCACGTTTG
     2581 GCAcATGCCT GCTTTGACCG AGATCTTTGG AGATGATTCT GTACTACAAT TCGGTGGAGG
     2641 AACTTTAGGC CACCCTTGGG GAAATGCACC GGGTGCCGTA GCCAACCGAG TAGCTCTGGA
     2701 AGCATGTGTA CAAGCTCGTA ATGAGGGACG TGATCTTGCA GTCGAGGGTA ATGAAATTAT
     2761 CCGTGAAGCT TGCAAATGGA GTCCTGAACT AGCTGCTGCT TGTGAAGTAT GGAAAGAGAT
     2821 CACATTTAAC TTCCCAACCA TCGATAAATT AGATGGCCAA GAGTAAATTA ACGGCACTcc
     2881 tcagccaagt caaaagcctc cgaccggagg cttttgacta catgcccatg gcgtttacgc
     2941 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     3001 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     3061 cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     3121 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     3181 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     3241 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     3301 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     3361 tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     3421 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     3481 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     3541 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     3601 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     3661 attatggtga aagttggaac ctcttacgtg ccattcaAAA TAGGCCGTtg agatcctttt
     3721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3781 ttgccggatc aagagctacc acctcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3841 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4081 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4261 ttgtgatgct cgtcaggggg gcggagccta tggaaa
//