LOCUS       pBL155C_-_[ColE1]_cCadC 4290 bp ds-DNA     circular     20-MAR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(428..619)
                     /label="Rop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     USER junction   716..724
                     /label="USER021"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     USER junction   718..729
                     /label="USER006"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      730..774
                     /label="rrnB1 short"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     USER junction   782..796
                     /label="USER008"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein bind... 816..834
                     /label="tetR"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     promoter        835..870
                     /label="Ptet"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     protein bind... 870..888
                     /label="tetR"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_fwdcolor="#f6989d"
     misc_feature    921..935
                     /label="USER004_BL"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     USER junction   927..935
                     /label="USER009"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             936..949
                     /label="sd8"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             950..1429
                     /label="cadC 1-155 Leu16 TM GCN4 ZIP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             950..1429
                     /label="Translation 950-1429"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLRPRLIDLLVFFAQHSGEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMVPVIWYSEEEGEEIMLSSAAAISEAVPATDSPSHSLNIQNTATPPEQSPVKSKRGGSGG"
     gene            953..1414
                     /label="cadC 1-155"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1031..1039
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1037..1039
                     /label="E30R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1307..1321
                     /label="AAAIS mutation"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1309..1317
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     USER junction   1420..1429
                     /label="USER"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            1430..2860
                     /label="NtRbcL ΔN1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1430..2860
                     /label="Translation 1430-2860"
                     /translation="MPQTETKASVGFKAGVKEYKLTYYTPEYQTKDTDILAAFRVTPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYRIERVVGEKDQYIAYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPAYVKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEALYKAQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLAHYCRDNGLLLHIHRAMHAVIDRQKNHGIHFRVLAKALRMSGGDHIHSGTVVGKLEGERDITLGFVDLLRDDFVEQDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVKARNEGRDLAQEGNEIIREACKWSPELAAACEVWKEIVFNFAAVDVLDK*"
     USER junction   2861..2872
                     /label="USER002"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      complement(2880..2915)
                     /label="p14/tonB bidirectional terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     terminator      complement(2882..2913)
                     /label="tonB"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     cloning         2916..2928
                     /label="fragment"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     USER junction   2916..2928
                     /label="USER005"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(2929..3588)
                     /label="cat"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     USER junction   complement(2958..2965)
                     /label="USER028"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(3589..3686)
                     /label="cat promoter"
                     /ApEinfo_revcolor="#c4df9b"
                     /ApEinfo_fwdcolor="#c4df9b"
     misc_feature    3692..3702
                     /label="USER030"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     origin          3703..4290
                     /label="colE1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
      661 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacaagagg
      721 acatccggtc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttgctgag
      781 gagacttagg gaccctccat cgaatggcca gatgatccct atcagtgata gagattgaca
      841 ctctatcatt gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga
      901 atagttcgac aaaaatctag acctgcAGGT GCAGTaaagg aaaaaaaaaa tgcaacaacc
      961 tgtagttcgc gttggcgaat ggcttgttac tccgtccata aaccaaatta gccgcaatgg
     1021 gcgtcaactt acccttCGTc cgagattaat cgatcttctg gttttctttg ctcaacacag
     1081 tggcgaagta cttagcaggg atgaacttat cgataatgtc tggaagagaa gtattgtcac
     1141 caatcacgtt gtgacgcaga gtatctcaga actacgtaag tcattaaaag ataatgatga
     1201 agatagtcct gtctatatcg ctactgtacc aaagcgcggc tataaattaa tggtgccggt
     1261 tatctggtac agcgaagaag agggagagga aataatgcta tcttcgGCAG CGGCTATCTC
     1321 Tgaggcggtt cctgccacag attctccctc ccacagtctt aacattcaaa acaccgcaac
     1381 gccacctgaa caatccccag ttaaaagcaa acgaggtggA TCTGGTGGTa tgccacaaac
     1441 agagactaaa gcaagtgttg gattcaaagc tggtgttaaa gagtacaaat tgacttatta
     1501 tactcctgag taccaaacca aggatactga tatattggca gcattccgag taactcctca
     1561 acctggagtt ccacctgaag aagcaggggc cgcggtagct gccgaatctt ctactggtac
     1621 atggacaact gtatggaccg atggacttac cagccttgat cgttacaaag ggcgatgcta
     1681 ccgcatcgag cgtgttgttg gagaaaaaga tcaatatatt gcttatgtag cttacccttt
     1741 agaccttttt gaagaaggtt ctgttaccaa catgtttact tccattgtag gtaacgtatt
     1801 tgggttcaaa gccctgcgcg ctctacgtct ggaagatctg cgaatccctc ctgcttatgt
     1861 taaaactttc caaggtccgc ctcatgggat ccaagttgaa agagataaat tgaacaagta
     1921 tggtcgtccc ctgttgggat gtactattaa acctaaattg gggttatctg ctaaaaacta
     1981 cggtagagct gtttatgaat gtcttcgcgg tggacttgat tttaccaaag atgatgagaa
     2041 cgtgaactca caaccattta tgcgttggag agatcgtttc ttattttgtg ccgaagcact
     2101 ttataaagca caggctgaaa caggtgaaat caaagggcat tacttgaatg ctactgcagg
     2161 tacatgcgaa gaaatgatca aaagagctgt atttgctaga gaattgggcg ttccgatcgt
     2221 aatgcatgac tacttaacgg ggggattcac cgcaaatact agcttggctc attattgccg
     2281 agataatggt ctacttcttc acatccaccg tgcaatgcat gcggttattg atagacagaa
     2341 gaatcatggt atccacttcc gggtattagc aaaagcgtta cgtatgtctg gtggagatca
     2401 tattcactct ggtaccgtag taggtaaact tgaaggtgaa agagacataa ctttgggctt
     2461 tgttgattta ctgcgtgatg attttgttga acaagatcga agtcgcggta tttatttcac
     2521 tcaagattgg gtctctttac caggtgttct acccgtggct tcaggaggta ttcacgtttg
     2581 gcatatgcct gctctgaccg agatctttgg ggatgattcc gtactacagt tcggtggagg
     2641 aactttagga catccttggg gtaatgcgcc aggtgccgta gctaatcgag tagctctaga
     2701 agcatgtgta aaagctcgta atgaaggacg tgatcttgct caggaaggta atgaaattat
     2761 tcgcgaggct tgcaaatgga gcccggaact agctgctgct tgtgaagtat ggaaagagat
     2821 cgtatttaat tttgcagcag tggacgtttt ggataagtaa ATTAACGGCA CTcctcagcc
     2881 aagtcaaaag cctccgaccg gaggcttttg actacatgcc catggcgttt acgccccgcc
     2941 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     3001 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     3061 atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     3121 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     3181 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     3241 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     3301 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     3361 tgccatacgg aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     3421 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     3481 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     3541 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     3601 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     3661 gtgaaagttg gaacctctta cgtgccattc aAAATAGGCC GTtgagatcc tttttttctg
     3721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3781 gatcaagagc taccacctct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3841 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4261 tgctcgtcag gggggcggag cctatggaaa
//