LOCUS       541_pEGFP_C1_RME       11483 bp    DNA     circular SYN 28-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Lobingier Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11483
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     611..636
                     /label=F
                     /note="color: black; sequence: 
                     TATAGCTAGCCACCCATGGTGAGCAAGGGCGAGGAGCTG"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1:  613 ..  615 / #05fd14
                        2:  616 ..  618 / #05fd14 / 1a
                        3:  619 .. 1329 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1312..1329)
                     /label=R
                     /note="color: black; sequence: 
                     TATAAAGCTTCTTGTACAGCTCGTCCAT"
     misc_feature    1330..1379
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
                     /note="color: #99ccff"
     primer_bind     1375..1432
                     /label=Primer 1
                     /note="color: black; sequence: 
                     TATAGGTACCGGAGGGGGTTCCGGCGGAGGCAGCaacataattagggaaaataaggatc
                     tgg; added: 2021-12-09"
     CDS             1405..7996
                     /codon_start=1
                     /label=hRME8
                     /note="color: #ff6600"
                     /translation="NIIRENKDLACFYTTKHSWRGKYKRVFSVGTHAITTYNPNTLEVT
                     NQWPYGDICSISPVGKGQGTEFNLTFRKGSGKKSETLKFSTEHRTELLTEALRFRTDFS
                     EGKITGRRYNCYKHHWSDSRKPVILEVTPGGFDQINPATNRVLCSYDYRNIEGFVDLSD
                     YQGGFCILYGGFSRLHLFASEQREEIIKSAIDHAGNYIGISLRIRKEPLEFEQYLNLRF
                     GKYSTDESITSLAEFVVQKISPRHSEPVKRVLALTETCLVERDPATYNIATLKPLGEVF
                     ALVCDSENPQLFTIEFIKGQVRKYSSTERDSLLASLLDGVRASGNRDVCVKMTPTHKGQ
                     RWGLLSMPVDEEVESLHLRFLATPPNGNFADAVFRFNANISYSGVLHAVTQDGLFSENK
                     EKLINNAITALLSQEGDVVASNAELESQFQAVRRLVASKAGFLAFTQLPKFRERLGVKV
                     VKALKRSNNGIIHAAVDMLCALMCPMHDDYDLRQEQLNKASLLSSKKFLENLLEKFNSH
                     VDHGTGALVISSLLDFLTFALCAPYSETTEGQQFDMLLEMVASNGRTLFKLFQHPSMAI
                     IKGAGLVMKAIIEEGDKEIATKMQELALSEGALPRHLHTAMFTISSDQRMLTNRQLSRH
                     LVGLWTADNATATNLLKRILPPGLLAYLESSDLVPEKDADRMHVRDNVKIAMDQYGKFN
                     KVPEWQRLAGKAAKEVEKFAKEKVDLVLMHWRDRMGIAQKENINQKPVVLRKRRQRIKI
                     EANWDLFYYRFGQDHARSNLIWNFKTREELKDTLESEMRAFNIDRELGSANVISWNHHE
                     FEVKYECLAEEIKIGDYYLRLLLEEDENEESGSIKRSYEFFNELYHRFLLTPKVNMKCL
                     CLQALAIVYGRCHEEIGPFTDTRYIIGMLERCTDKLERDRLILFLNKLILNKKNVKDLM
                     DSNGIRILVDLLTLAHLHVSRATVPLQSNVIEAAPDMKRESEKEWYFGNADKERSGPYG
                     FHEMQELWTKGMLNAKTRCWAQGMDGWRPLQSIPQLKWCLLASGQAVLNETDLATLILN
                     MLITMCGYFPSRDQDNAIIRPLPKVKRLLSDSTCLPHIIQLLLTFDPILVEKVAILLYH
                     IMQDNPQLPRLYLSGVFFFIMMYTGSNVLPVARFLKYTHTKQAFKSEETKGQDIFQRSI
                     LGHILPEAMVCYLENYEPEKFSEIFLGEFDTPEAIWSSEMRRLMIEKIAAHLADFTPRL
                     QSNTRALYQYCPIPIINYPQLENELFCNIYYLKQLCDTLRFPDWPIKDPVKLLKDTLDA
                     WKKEVEKKPPMMSIDDAYEVLNLPQGQGPHDESKIRKAYFRLAQKYHPDKNPEGRDMFE
                     KVNKAYEFLCTKSAKIVDGPDPENIILILKTQSILFNRHKEDLQPYKYAGYPMLIRTIT
                     METSDDLLFSKESPLLPAATELAFHTVNCSALNAEELRRENGLEVLQEAFSRCVAVLTR
                     ASKPSDMSVQVCGYISKCYSVAAQFEECREKITEMPSIIKDLCRVLYFGKSIPRVAALG
                     VECVSSFAVDFWLQTHLFQAGILWYLLGFLFNYDYTLEESGIQKSEETNQQEVANSLAK
                     LSVHALSRLGGYLAEEQATPENPTIRKSLAGMLTPYVARKLAVASVTEILKMLNSNTES
                     PYLIWNNSTRAELLEFLESQQENMIKKGDCDKTYGSEFVYSDHAKELIVGEIFVRVYNE
                     VPTFQLEVPKAFAASLLDYIGSQAQYLHTFMAITHAAKVESEQHGDRLPRVEMALEALR
                     NVIKYNPGSESECIGHFKLIFSLLRVHGAGQVQQLALEVVNIVTSNQDCVNNIAESMVL
                     SSLLALLHSLPSSRQLVLETLYALTSSTKIIKEAMAKGALIYLLDMFCNSTHPQVRAQT
                     AELFAKMTADKLIGPKVRITLMKFLPSVFMDAMRDNPEAAVHIFEGTHENPELIWNDNS
                     RDKVSTTVREMMLEHFKNQQDNPEANWKLPEDFAVVFGEAEGELAVGGVFLRIFIAQPA
                     WVLRKPREFLIALLEKLTELLEKNNPHGETLETLTMATVCLFSAQPQLADQVPPLGHLP
                     KVIQAMNHRNNAIPKSAIRVIHALSENELCVRAMASLETIGPLMNGMKKRADTVGLACE
                     AINRMFQKEQSELVAQALKADLVPYLLKLLEGIGLENLDSPAATKAQIVKALKAMTRSL
                     QYGEQVNEILCRSSVWSAFKDQKHDLFI"
     primer_bind     2230..2257
                     /label=1
                     /note="color: black; sequence: 
                     CCTTTAGGAGAAGTATTTGCGTTGGTCT; added: 2021-11-29"
     primer_bind     3030..3057
                     /label=2
                     /note="color: black; sequence: 
                     TAGTGAGACAACTGAAGGGCAGCAGTTT; added: 2021-11-29"
     primer_bind     complement(3697..3720)
                     /label=778_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAgtttgacctggcatggtcttgacc; added: 2021-11-12"
     primer_bind     3818..3841
                     /label=3
                     /note="color: black; sequence: TGATCTCCTGGAACCACCATGAGT; 
                     added: 2021-11-29"
     primer_bind     4613..4639
                     /label=4
                     /note="color: black; sequence: TACCCAAAGTGAAAAGACTGCTGTCAG;
                     added: 2021-11-29"
     primer_bind     complement(4962..5001)
                     /label=1205_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAttctggagtatcaaattctcctagaaaaatctcagaaaac; 
                     added: 2021-11-12"
     primer_bind     5412..5441
                     /label=5
                     /note="color: black; sequence: 
                     AGAAGGGAGGGACATGTTTGAAAAAGTAAA; added: 2021-11-29"
     primer_bind     6220..6242
                     /label=6
                     /note="color: black; sequence: AAAAGCTTAGCTGGCATGCTGAC; 
                     added: 2021-12-09"
     primer_bind     complement(6291..6325)
                     /label=1646_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAatatggactttctgtgttgctgttaagcatcttc; added: 
                     2021-11-12"
     primer_bind     complement(6814..6840)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     TATAGGATCCTCAtgattcagcaatattgttgacacagtc; added: 
                     2021-10-29"
     primer_bind     7018..7041
                     /label=7
                     /note="color: black; sequence: GCCCAAACAGCAGAACTTTTTGCC; 
                     added: 2021-11-29"
     primer_bind     complement(7144..7183)
                     /label=1932_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCActcaggattttcatgagttccttcaaaaatatgtacagc; 
                     added: 2021-11-12"
     primer_bind     complement(7963..7996)
                     /label=2204_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAaatgaacaaatcatgtttctgatctttgaaggc; added: 
                     2021-12-09"
     primer_bind     7971..8001
                     /label=CT_Add_F
                     /note="color: black; sequence: 
                     agatcagaaacatgatttgttcatttctgag; added: 2023-06-06"
     primer_bind     complement(8112..8133)
                     /label=CT_Add_R
                     /note="color: black; sequence: 
                     GCCCTCTAGACTCGAGCGGCCTTAATTAATCAagtctgatagccaaggtcg; added:
                     2023-06-06"
     misc_feature    8147
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
                     /note="color: #99ccff"
     misc_feature    8156..8166
                     /label=stop codons
                     /note="stop codons in all three reading frames"
                     /note="color: #ff0000"
     polyA_signal    8271..8392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(8399..8854)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        8881..8985
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        8987..9344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      9195..9330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             9379..10173
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    10405..10452
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      10781..11369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 ggagggggtt ccggcggagg cagcaacata attagggaaa ataaggatct ggcatgtttc
     1441 tacacaacaa aacattcatg gagggggaag tataagcgtg tcttttcagt tggaactcat
     1501 gcgattacta catataatcc caatacctta gaagtaacaa atcagtggcc ttatggagac
     1561 atttgcagca tcagccctgt tggaaaagga caaggaacgg agttcaacct cacatttcgt
     1621 aaaggcagtg gaaaaaagtc agaaacttta aaattttcta cagagcacag aacagaactt
     1681 cttacagaag cattgagatt tagaactgat ttttcagagg gaaaaatcac aggaaggaga
     1741 tacaactgct ataagcatca ctggagtgac tcaagaaaac ctgtaatttt ggaagtaact
     1801 ccaggaggct ttgaccaaat taatcctgca accaacagag tactctgttc ctatgactat
     1861 agaaatattg aaggatttgt agatctctca gattatcaag gaggattttg tatactttat
     1921 ggaggattta gtagattgca tttatttgcg tcagagcaaa gagaagagat tattaaaagt
     1981 gcaatagacc atgctggtaa ctacataggt atttcattgc ggatcaggaa agagccttta
     2041 gaattcgagc aatatttgaa tcttcgcttt ggaaaataca gcactgatga atccatcaca
     2101 tctttagcag agtttgtagt ccaaaaaata tcacctagac attcggagcc tgttaaaaga
     2161 gttctagcac ttacagaaac atgtttagta gaacgtgatc cggcaaccta taatattgca
     2221 acattgaagc ctttaggaga agtatttgcg ttggtctgtg actcagaaaa tccacaactt
     2281 tttaccattg aatttataaa agggcaagta cggaaatatt cttcaacaga gagagattcc
     2341 ttattagcaa gtttgctgga tggagtaaga gcctctggta atagagatgt ttgtgtaaaa
     2401 atgacaccaa cccataaagg tcagcgatgg gggttactca gcatgcctgt tgatgaggaa
     2461 gtagagagcc ttcacctcag gttcttagct acgcctccaa atggcaactt tgcagatgct
     2521 gtattcaggt tcaatgctaa tatttcatac agtggagtcc tacatgcagt aacacaggat
     2581 ggtctcttct cagaaaacaa agaaaaactg atcaataatg ccataacagc attactgtcc
     2641 caagaagggg atgtcgttgc ttcaaatgcg gaacttgaga gtcagttcca ggctgtgagg
     2701 aggcttgtgg catccaaagc tggtttcctg gctttcactc agcttccaaa gtttcgcgag
     2761 cgtctagggg tgaaggtagt aaaagcactc aaaagaagca acaacggaat aatccatgca
     2821 gcagttgata tgctttgtgc ccttatgtgt cccatgcatg atgactatga cttaagacaa
     2881 gaacagttga acaaagcttc tcttctctcg tcaaagaagt ttctggaaaa cttactggag
     2941 aaatttaatt cccatgtgga tcatgggact ggtgccctag ttattagttc gctcttggac
     3001 ttccttacct ttgccctctg tgctccatat agtgagacaa ctgaagggca gcagtttgat
     3061 atgctcttgg agatggtagc atccaatgga agaacccttt ttaaactttt tcagcatcct
     3121 tccatggcaa taataaaagg agctgggttg gttatgaagg caataataga ggaaggtgat
     3181 aaagaaattg ctacaaaaat gcaggagctt gccctaagtg aaggtgcctt acctcgacac
     3241 ttgcatactg cgatgtttac aataagctca gatcaaagga tgcttacaaa tagacagcta
     3301 agtagacatt tagtgggact ctggacagct gataatgcaa ctgcaacaaa cttgttgaaa
     3361 cgcattttgc cgccaggctt gctggcatac ttggaaagct cagatctcgt acctgagaag
     3421 gatgctgatc ggatgcatgt tagagacaat gtgaaaatag caatggatca gtatggaaaa
     3481 tttaataaag ttccagagtg gcaaagacta gctggaaaag ctgctaaaga agttgaaaaa
     3541 tttgccaaag aaaaagtgga tcttgtattg atgcactgga gggataggat gggcattgct
     3601 caaaaagaga atataaatca gaagccagtg gttcttcgaa agagaagaca aagaataaaa
     3661 atagaagcaa attgggatct cttctattat aggtttggtc aagaccatgc caggtcaaac
     3721 cttatttgga atttcaaaac acgagaagaa ctgaaagata ctcttgaatc tgaaatgaga
     3781 gcatttaata ttgacagaga acttggaagt gcaaatgtga tctcctggaa ccaccatgag
     3841 tttgaggtta aatatgagtg cctggcagag gaaattaaaa taggagacta ttacctgaga
     3901 ctactattgg aggaagatga gaatgaagaa agtggatcaa ttaagagatc gtatgaattt
     3961 ttcaatgagc tttatcatcg cttcttgctc accccaaaag taaacatgaa gtgtttatgt
     4021 ttacaagccc ttgctattgt ttatggcaga tgtcacgaag aaataggacc ttttacagat
     4081 accagatata tcattggaat gttagagagg tgcacagata aacttgaacg agataggttg
     4141 attctcttcc ttaacaagtt gatccttaat aagaaaaatg ttaaggatct catggattca
     4201 aatggaataa gaatccttgt ggacttgctt acccttgcac atctccatgt aagccgagct
     4261 acagtaccac tgcaaagcaa tgtaattgaa gctgctccag atatgaaaag agagagtgaa
     4321 aaggaatggt attttggcaa cgcagacaaa gaaaggagtg gcccgtatgg atttcatgag
     4381 atgcaagaat tgtggaccaa aggaatgtta aatgcaaaaa ccagatgctg ggctcaaggc
     4441 atggatggat ggcgaccact tcagtccata ccccagctta agtggtgtct cttagccagt
     4501 ggacaggctg tcctgaatga aactgacctt gctaccctta tattgaacat gttgatcaca
     4561 atgtgtggat attttccaag cagggatcaa gacaatgcca tcattcggcc tctacccaaa
     4621 gtgaaaagac tgctgtcaga tagcacttgc cttccccata ttattcagct actgctgacc
     4681 tttgacccta tccttgttga gaaggttgct attttgttat accatatcat gcaagataac
     4741 ccacagttac cccgccttta tctgagtgga gtatttttct ttatcatgat gtacacaggt
     4801 tccaatgtgc ttcctgttgc tcgatttttg aaatacacac ataccaaaca ggctttcaag
     4861 tcagaagaga caaaaggaca agatattttt cagagaagta tacttgggca cattctacct
     4921 gaagcaatgg tttgttactt agaaaattat gaacctgaaa agttttctga gatttttcta
     4981 ggagaatttg atactccaga agcaatctgg agcagtgaaa tgaggcgcct gatgatagag
     5041 aagattgctg cccatctcgc ggatttcaca cctcgtcttc agagtaacac aagagcactt
     5101 tatcagtatt gccccattcc tataatcaac tatccacaac tcgaaaatga actattttgt
     5161 aatatttatt acctcaaaca actgtgtgat acactccggt ttccagattg gccaattaaa
     5221 gacccggtta agcttctaaa agataccctt gatgcctgga agaaagaagt agaaaagaag
     5281 ccacctatga tgtcaataga tgatgcttat gaagtgctta atctgcctca aggacaggga
     5341 ccgcatgatg agagcaagat taggaaagct tacttcagac ttgcacaaaa gtaccaccct
     5401 gataagaatc cagaagggag ggacatgttt gaaaaagtaa ataaagcata tgaattttta
     5461 tgtaccaaat cagcaaaaat agtggatggg ccagatccag agaatataat tttaattcta
     5521 aaaacacaga gcatcctctt caaccgtcat aaagaagatt tacagcctta taaatatgca
     5581 ggatacccca tgcttattcg gactataaca atggaaactt cagatgacct ccttttctca
     5641 aaagaatcac cattgttgcc tgcggctaca gagctagctt tccatactgt caactgttca
     5701 gccctcaatg ctgaagagct cagaagagag aatggactag aggtgttaca agaggcattt
     5761 agtcgctgtg tggctgtctt gactcgtgct agtaaaccaa gtgacatgtc agtacaggtg
     5821 tgtggataca taagtaaatg ctacagtgtg gctgctcagt ttgaggaatg ccgagagaag
     5881 atcacggaaa tgcctagcat catcaaggat ctctgtcggg tactatattt tggcaagagt
     5941 attccccgcg tagctgctct tggggtagaa tgtgtcagtt cttttgctgt ggatttctgg
     6001 ctacagacac acctatttca ggctggaatt ttgtggtatc tccttggttt tctgtttaat
     6061 tatgactaca cactagaaga gagtggcatt cagaaaagtg aagaaacaaa ccagcaggag
     6121 gtagcaaaca gccttgccaa actgagtgtc catgctctga gtcgccttgg agggtatttg
     6181 gctgaagaac aagcaactcc agaaaatcca accataagga aaagcttagc tggcatgctg
     6241 acaccctatg ttgctagaaa acttgctgtg gctagtgtga ctgagatttt gaagatgctt
     6301 aacagcaaca cagaaagtcc atatttgata tggaacaatt ctacaagagc agaattactt
     6361 gaatttcttg aatcccaaca agaaaacatg attaaaaaag gtgattgtga caaaacttat
     6421 ggatcagaat ttgtctacag tgatcatgcc aaagaactta ttgtagggga gatttttgtt
     6481 agggtgtata atgaagttcc tactttccaa ctggaggttc caaaagcatt tgctgcaagt
     6541 ctcttggatt atataggctc gcaggcccaa tacttgcaca cattcatggc catcacacac
     6601 gcggcaaaag tggagtcaga gcaacatgga gatcgcttac cgagagtaga aatggctttg
     6661 gaggctctga gaaatgtcat aaaatacaat ccaggttctg agagtgaatg cattgggcac
     6721 tttaagttga tattttctct tctccgagtt catggagctg gtcaagtgca gcagttggct
     6781 ttagaggttg tgaatatagt gacatctaac caagactgtg tcaacaatat tgctgaatca
     6841 atggttttgt ccagtttatt ggctcttcta cattcattgc catcaagtcg tcagcttgtt
     6901 ctggaaactc tttatgcttt gacatcgagt acaaaaataa tcaaagaagc aatggcaaag
     6961 ggtgctttga tctatttact ggatatgttc tgcaattcaa cacatccaca ggttcgagcc
     7021 caaacagcag aactttttgc caaaatgaca gcagataaac tgataggtcc aaaggttcga
     7081 attacgttaa tgaaatttct accaagcgtt ttcatggatg ctatgagaga caatcctgaa
     7141 gctgctgtac atatttttga aggaactcat gaaaatcctg agttaatttg gaatgataat
     7201 tccagagata aagtgtccac aacagttagg gaaatgatgc tagagcactt taaaaatcag
     7261 caggacaacc ctgaggcaaa ctggaagttg cctgaagatt ttgctgtggt gtttggagaa
     7321 gcagagggtg aacttgctgt tggaggagtc ttcttgagga tctttattgc acaaccagcc
     7381 tgggttctaa gaaagcctag agaatttctt attgccctgt tagaaaaatt aactgagctc
     7441 ctagagaaga acaatcctca tggagaaact ctggaaacct tgacaatggc aacagtgtgt
     7501 ctcttcagcg cacaacctca gctggcagat caggtcccgc cattgggcca tcttcccaaa
     7561 gttatccagg caatgaatca taggaacaat gccattccta agagtgccat tcgggttatc
     7621 catgccttgt ctgaaaatga gctgtgtgtt cgagccatgg catctttaga gaccattggc
     7681 ccactgatga atggaatgaa aaagcgagca gatactgttg gtctagcctg tgaagcaatt
     7741 aatcgaatgt ttcagaagga gcagagtgaa ttagtagcac aagccctgaa agcagatttg
     7801 gttccatacc tcttaaaatt actcgaaggc attggccttg aaaacctgga cagcccagca
     7861 gccactaagg ctcagattgt taaagctctc aaggcaatga ctcgaagttt gcagtatgga
     7921 gaacaggtga atgaaatcct gtgccgttct tcagtctgga gtgccttcaa agatcagaaa
     7981 catgatttgt tcatttctga gtcacaaaca gcaggagcag ctgcaggacc tggagttgct
     8041 ggctacctta ccgcaggtac atctacatca gtcatgtcta acctgccacc tcctgtagac
     8101 catgaggcag gcgaccttgg ctatcagact tgagcggccg cggatccacc ggatctagat
     8161 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     8221 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     8281 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     8401 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     8461 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8521 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8581 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8641 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8701 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8761 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8821 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     8881 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8941 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     9001 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     9061 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     9121 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     9181 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     9241 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9301 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     9361 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     9421 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     9481 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     9541 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     9601 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     9661 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     9721 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     9781 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     9841 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     9901 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     9961 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    10021 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    10081 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    10141 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
    10201 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
    10261 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    10321 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
    10381 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
    10441 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
    10501 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
    10561 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
    10621 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10681 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10741 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10801 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10861 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10921 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10981 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    11041 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    11101 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11161 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    11221 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11281 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11341 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11401 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    11461 ataaccgtat taccgccatg cat
//
