LOCUS       581_pEGFP_C1_RME       11348 bp    DNA     circular SYN 28-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Lobingier Lab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 28, 2025 from SnapGene 8.1.0
            https://www.snapgene.com
COMMENT     Sequence Label: pEGFP-C1-DNAJC13(2198t+hpd)
FEATURES             Location/Qualifiers
     source          1..11348
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     581..615
                     /label=HPDaaa Fwd
                     /note="color: black; sequence: 
                     CGTCAGATCCGCTAGCGCTACCGGTCGCCACCATG; added: 2023-05-08"
     primer_bind     611..636
                     /label=F
                     /note="color: black; sequence: 
                     TATAGCTAGCCACCCATGGTGAGCAAGGGCGAGGAGCTG"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1:  613 ..  615 / #05fd14
                        2:  616 ..  618 / #05fd14 / 1a
                        3:  619 .. 1329 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     733..743
                     /label=6
                     /note="color: black; sequence: AAAAGCTTAGCTGGCATGCTGAC; 
                     added: 2021-11-29"
     primer_bind     complement(1312..1329)
                     /label=R
                     /note="color: black; sequence: 
                     TATAAAGCTTCTTGTACAGCTCGTCCAT"
     misc_feature    1330..1379
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
                     /note="color: #99ccff"
     primer_bind     1375..1432
                     /label=Primer 1
                     /note="color: black; sequence: 
                     TATAGGTACCGGAGGGGGTTCCGGCGGAGGCAGCaacataattagggaaaataaggatc
                     tgg; added: 2021-12-09"
     CDS             1405..7996
                     /codon_start=1
                     /label=hRME8
                     /note="color: #ff6600"
                     /translation="NIIRENKDLACFYTTKHSWRGKYKRVFSVGTHAITTYNPNTLEVT
                     NQWPYGDICSISPVGKGQGTEFNLTFRKGSGKKSETLKFSTEHRTELLTEALRFRTDFS
                     EGKITGRRYNCYKHHWSDSRKPVILEVTPGGFDQINPATNRVLCSYDYRNIEGFVDLSD
                     YQGGFCILYGGFSRLHLFASEQREEIIKSAIDHAGNYIGISLRIRKEPLEFEQYLNLRF
                     GKYSTDESITSLAEFVVQKISPRHSEPVKRVLALTETCLVERDPATYNIATLKPLGEVF
                     ALVCDSENPQLFTIEFIKGQVRKYSSTERDSLLASLLDGVRASGNRDVCVKMTPTHKGQ
                     RWGLLSMPVDEEVESLHLRFLATPPNGNFADAVFRFNANISYSGVLHAVTQDGLFSENK
                     EKLINNAITALLSQEGDVVASNAELESQFQAVRRLVASKAGFLAFTQLPKFRERLGVKV
                     VKALKRSNNGIIHAAVDMLCALMCPMHDDYDLRQEQLNKASLLSSKKFLENLLEKFNSH
                     VDHGTGALVISSLLDFLTFALCAPYSETTEGQQFDMLLEMVASNGRTLFKLFQHPSMAI
                     IKGAGLVMKAIIEEGDKEIATKMQELALSEGALPRHLHTAMFTISSDQRMLTNRQLSRH
                     LVGLWTADNATATNLLKRILPPGLLAYLESSDLVPEKDADRMHVRDNVKIAMDQYGKFN
                     KVPEWQRLAGKAAKEVEKFAKEKVDLVLMHWRDRMGIAQKENINQKPVVLRKRRQRIKI
                     EANWDLFYYRFGQDHARSNLIWNFKTREELKDTLESEMRAFNIDRELGSANVISWNHHE
                     FEVKYECLAEEIKIGDYYLRLLLEEDENEESGSIKRSYEFFNELYHRFLLTPKVNMKCL
                     CLQALAIVYGRCHEEIGPFTDTRYIIGMLERCTDKLERDRLILFLNKLILNKKNVKDLM
                     DSNGIRILVDLLTLAHLHVSRATVPLQSNVIEAAPDMKRESEKEWYFGNADKERSGPYG
                     FHEMQELWTKGMLNAKTRCWAQGMDGWRPLQSIPQLKWCLLASGQAVLNETDLATLILN
                     MLITMCGYFPSRDQDNAIIRPLPKVKRLLSDSTCLPHIIQLLLTFDPILVEKVAILLYH
                     IMQDNPQLPRLYLSGVFFFIMMYTGSNVLPVARFLKYTHTKQAFKSEETKGQDIFQRSI
                     LGHILPEAMVCYLENYEPEKFSEIFLGEFDTPEAIWSSEMRRLMIEKIAAHLADFTPRL
                     QSNTRALYQYCPIPIINYPQLENELFCNIYYLKQLCDTLRFPDWPIKDPVKLLKDTLDA
                     WKKEVEKKPPMMSIDDAYEVLNLPQGQGPHDESKIRKAYFRLAQKYAAAKNPEGRDMFE
                     KVNKAYEFLCTKSAKIVDGPDPENIILILKTQSILFNRHKEDLQPYKYAGYPMLIRTIT
                     METSDDLLFSKESPLLPAATELAFHTVNCSALNAEELRRENGLEVLQEAFSRCVAVLTR
                     ASKPSDMSVQVCGYISKCYSVAAQFEECREKITEMPSIIKDLCRVLYFGKSIPRVAALG
                     VECVSSFAVDFWLQTHLFQAGILWYLLGFLFNYDYTLEESGIQKSEETNQQEVANSLAK
                     LSVHALSRLGGYLAEEQATPENPTIRKSLAGMLTPYVARKLAVASVTEILKMLNSNTES
                     PYLIWNNSTRAELLEFLESQQENMIKKGDCDKTYGSEFVYSDHAKELIVGEIFVRVYNE
                     VPTFQLEVPKAFAASLLDYIGSQAQYLHTFMAITHAAKVESEQHGDRLPRVEMALEALR
                     NVIKYNPGSESECIGHFKLIFSLLRVHGAGQVQQLALEVVNIVTSNQDCVNNIAESMVL
                     SSLLALLHSLPSSRQLVLETLYALTSSTKIIKEAMAKGALIYLLDMFCNSTHPQVRAQT
                     AELFAKMTADKLIGPKVRITLMKFLPSVFMDAMRDNPEAAVHIFEGTHENPELIWNDNS
                     RDKVSTTVREMMLEHFKNQQDNPEANWKLPEDFAVVFGEAEGELAVGGVFLRIFIAQPA
                     WVLRKPREFLIALLEKLTELLEKNNPHGETLETLTMATVCLFSAQPQLADQVPPLGHLP
                     KVIQAMNHRNNAIPKSAIRVIHALSENELCVRAMASLETIGPLMNGMKKRADTVGLACE
                     AINRMFQKEQSELVAQALKADLVPYLLKLLEGIGLENLDSPAATKAQIVKALKAMTRSL
                     QYGEQVNEILCRSSVWSAFKDQKHDLFI"
     primer_bind     2230..2257
                     /label=1
                     /note="color: black; sequence: 
                     CCTTTAGGAGAAGTATTTGCGTTGGTCT; added: 2021-11-29"
     primer_bind     2588..2598
                     /label=1205_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAttctggagtatcaaattctcctagaaaaatctcagaaaac; 
                     added: 2021-11-12"
     primer_bind     3030..3057
                     /label=2
                     /note="color: black; sequence: 
                     TAGTGAGACAACTGAAGGGCAGCAGTTT; added: 2021-11-29"
     primer_bind     complement(3697..3720)
                     /label=778_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAgtttgacctggcatggtcttgacc; added: 2021-11-12"
     primer_bind     3818..3841
                     /label=3
                     /note="color: black; sequence: TGATCTCCTGGAACCACCATGAGT; 
                     added: 2021-11-29"
     primer_bind     4613..4639
                     /label=4
                     /note="color: black; sequence: TACCCAAAGTGAAAAGACTGCTGTCAG;
                     added: 2021-11-29"
     primer_bind     complement(4962..5001)
                     /label=1205_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAttctggagtatcaaattctcctagaaaaatctcagaaaac; 
                     added: 2021-11-12"
     primer_bind     complement(5384..5423)
                     /label=HPDaaa rev
                     /note="color: black; sequence: 
                     tccctcccttctggattcttGGCAGCTGCgtacttttgtg; added: 
                     2023-05-08"
     primer_bind     5412..5441
                     /label=5
                     /note="color: black; sequence: 
                     AGAAGGGAGGGACATGTTTGAAAAAGTAAA; added: 2021-11-29"
     primer_bind     6220..6242
                     /label=6
                     /note="color: black; sequence: AAAAGCTTAGCTGGCATGCTGAC; 
                     added: 2021-11-29"
     primer_bind     complement(6291..6325)
                     /label=1646_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAatatggactttctgtgttgctgttaagcatcttc; added: 
                     2021-11-12"
     primer_bind     complement(6814..6840)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     TATAGGATCCTCAtgattcagcaatattgttgacacagtc; added: 
                     2021-10-29"
     primer_bind     7018..7041
                     /label=7
                     /note="color: black; sequence: GCCCAAACAGCAGAACTTTTTGCC; 
                     added: 2021-11-29"
     primer_bind     7143..7182
                     /label=rme8hpd fwd
                     /note="color: black; sequence: 
                     tgctgtacatatttttgaaggaactcatgaaaatcctgag; added: 
                     2023-07-10"
     primer_bind     7143..7182
                     /label=1927to end fwd
                     /note="color: black; sequence: 
                     tgctgtacatatttttgaaggaactcatgaaaatcctgag; added: 
                     2023-07-10"
     primer_bind     complement(7144..7183)
                     /label=1932_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCActcaggattttcatgagttccttcaaaaatatgtacagc; 
                     added: 2021-12-09"
     primer_bind     complement(7963..7999)
                     /label=2204_R
                     /note="color: black; sequence: 
                     TATAGGATCCTCAaatgaacaaatcatgtttctgatctttgaaggc; added: 
                     2021-12-09"
     primer_bind     complement(7974..8031)
                     /label=2198 rev
                     /note="color: black; sequence: 
                     TCAGTTATCTAGATCCGGTGGATCCgcggccgcTCAaatgaacaaatcatgtttctga;
                      added: 2023-07-10"
     misc_feature    8000..8005
                     /label=added noti site
                     /note="color: #a6acb3"
     misc_feature    8008..8012
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
                     /note="color: #99ccff"
     misc_feature    8021..8031
                     /label=stop codons
                     /note="stop codons in all three reading frames"
                     /note="color: #ff0000"
     polyA_signal    8136..8257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(8264..8719)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        8746..8850
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        8852..9209
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      9060..9195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             9244..10038
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9831..9838)
                     /label=HPDaaa Fwd
                     /note="color: black; sequence: 
                     CGTCAGATCCGCTAGCGCTACCGGTCGCCACCATG; added: 2023-05-08"
     misc_feature    10270..10317
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      10646..11234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 ggagggggtt ccggcggagg cagcaacata attagggaaa ataaggatct ggcatgtttc
     1441 tacacaacaa aacattcatg gagggggaag tataagcgtg tcttttcagt tggaactcat
     1501 gcgattacta catataatcc caatacctta gaagtaacaa atcagtggcc ttatggagac
     1561 atttgcagca tcagccctgt tggaaaagga caaggaacgg agttcaacct cacatttcgt
     1621 aaaggcagtg gaaaaaagtc agaaacttta aaattttcta cagagcacag aacagaactt
     1681 cttacagaag cattgagatt tagaactgat ttttcagagg gaaaaatcac aggaaggaga
     1741 tacaactgct ataagcatca ctggagtgac tcaagaaaac ctgtaatttt ggaagtaact
     1801 ccaggaggct ttgaccaaat taatcctgca accaacagag tactctgttc ctatgactat
     1861 agaaatattg aaggatttgt agatctctca gattatcaag gaggattttg tatactttat
     1921 ggaggattta gtagattgca tttatttgcg tcagagcaaa gagaagagat tattaaaagt
     1981 gcaatagacc atgctggtaa ctacataggt atttcattgc ggatcaggaa agagccttta
     2041 gaattcgagc aatatttgaa tcttcgcttt ggaaaataca gcactgatga atccatcaca
     2101 tctttagcag agtttgtagt ccaaaaaata tcacctagac attcggagcc tgttaaaaga
     2161 gttctagcac ttacagaaac atgtttagta gaacgtgatc cggcaaccta taatattgca
     2221 acattgaagc ctttaggaga agtatttgcg ttggtctgtg actcagaaaa tccacaactt
     2281 tttaccattg aatttataaa agggcaagta cggaaatatt cttcaacaga gagagattcc
     2341 ttattagcaa gtttgctgga tggagtaaga gcctctggta atagagatgt ttgtgtaaaa
     2401 atgacaccaa cccataaagg tcagcgatgg gggttactca gcatgcctgt tgatgaggaa
     2461 gtagagagcc ttcacctcag gttcttagct acgcctccaa atggcaactt tgcagatgct
     2521 gtattcaggt tcaatgctaa tatttcatac agtggagtcc tacatgcagt aacacaggat
     2581 ggtctcttct cagaaaacaa agaaaaactg atcaataatg ccataacagc attactgtcc
     2641 caagaagggg atgtcgttgc ttcaaatgcg gaacttgaga gtcagttcca ggctgtgagg
     2701 aggcttgtgg catccaaagc tggtttcctg gctttcactc agcttccaaa gtttcgcgag
     2761 cgtctagggg tgaaggtagt aaaagcactc aaaagaagca acaacggaat aatccatgca
     2821 gcagttgata tgctttgtgc ccttatgtgt cccatgcatg atgactatga cttaagacaa
     2881 gaacagttga acaaagcttc tcttctctcg tcaaagaagt ttctggaaaa cttactggag
     2941 aaatttaatt cccatgtgga tcatgggact ggtgccctag ttattagttc gctcttggac
     3001 ttccttacct ttgccctctg tgctccatat agtgagacaa ctgaagggca gcagtttgat
     3061 atgctcttgg agatggtagc atccaatgga agaacccttt ttaaactttt tcagcatcct
     3121 tccatggcaa taataaaagg agctgggttg gttatgaagg caataataga ggaaggtgat
     3181 aaagaaattg ctacaaaaat gcaggagctt gccctaagtg aaggtgcctt acctcgacac
     3241 ttgcatactg cgatgtttac aataagctca gatcaaagga tgcttacaaa tagacagcta
     3301 agtagacatt tagtgggact ctggacagct gataatgcaa ctgcaacaaa cttgttgaaa
     3361 cgcattttgc cgccaggctt gctggcatac ttggaaagct cagatctcgt acctgagaag
     3421 gatgctgatc ggatgcatgt tagagacaat gtgaaaatag caatggatca gtatggaaaa
     3481 tttaataaag ttccagagtg gcaaagacta gctggaaaag ctgctaaaga agttgaaaaa
     3541 tttgccaaag aaaaagtgga tcttgtattg atgcactgga gggataggat gggcattgct
     3601 caaaaagaga atataaatca gaagccagtg gttcttcgaa agagaagaca aagaataaaa
     3661 atagaagcaa attgggatct cttctattat aggtttggtc aagaccatgc caggtcaaac
     3721 cttatttgga atttcaaaac acgagaagaa ctgaaagata ctcttgaatc tgaaatgaga
     3781 gcatttaata ttgacagaga acttggaagt gcaaatgtga tctcctggaa ccaccatgag
     3841 tttgaggtta aatatgagtg cctggcagag gaaattaaaa taggagacta ttacctgaga
     3901 ctactattgg aggaagatga gaatgaagaa agtggatcaa ttaagagatc gtatgaattt
     3961 ttcaatgagc tttatcatcg cttcttgctc accccaaaag taaacatgaa gtgtttatgt
     4021 ttacaagccc ttgctattgt ttatggcaga tgtcacgaag aaataggacc ttttacagat
     4081 accagatata tcattggaat gttagagagg tgcacagata aacttgaacg agataggttg
     4141 attctcttcc ttaacaagtt gatccttaat aagaaaaatg ttaaggatct catggattca
     4201 aatggaataa gaatccttgt ggacttgctt acccttgcac atctccatgt aagccgagct
     4261 acagtaccac tgcaaagcaa tgtaattgaa gctgctccag atatgaaaag agagagtgaa
     4321 aaggaatggt attttggcaa cgcagacaaa gaaaggagtg gcccgtatgg atttcatgag
     4381 atgcaagaat tgtggaccaa aggaatgtta aatgcaaaaa ccagatgctg ggctcaaggc
     4441 atggatggat ggcgaccact tcagtccata ccccagctta agtggtgtct cttagccagt
     4501 ggacaggctg tcctgaatga aactgacctt gctaccctta tattgaacat gttgatcaca
     4561 atgtgtggat attttccaag cagggatcaa gacaatgcca tcattcggcc tctacccaaa
     4621 gtgaaaagac tgctgtcaga tagcacttgc cttccccata ttattcagct actgctgacc
     4681 tttgacccta tccttgttga gaaggttgct attttgttat accatatcat gcaagataac
     4741 ccacagttac cccgccttta tctgagtgga gtatttttct ttatcatgat gtacacaggt
     4801 tccaatgtgc ttcctgttgc tcgatttttg aaatacacac ataccaaaca ggctttcaag
     4861 tcagaagaga caaaaggaca agatattttt cagagaagta tacttgggca cattctacct
     4921 gaagcaatgg tttgttactt agaaaattat gaacctgaaa agttttctga gatttttcta
     4981 ggagaatttg atactccaga agcaatctgg agcagtgaaa tgaggcgcct gatgatagag
     5041 aagattgctg cccatctcgc ggatttcaca cctcgtcttc agagtaacac aagagcactt
     5101 tatcagtatt gccccattcc tataatcaac tatccacaac tcgaaaatga actattttgt
     5161 aatatttatt acctcaaaca actgtgtgat acactccggt ttccagattg gccaattaaa
     5221 gacccggtta agcttctaaa agataccctt gatgcctgga agaaagaagt agaaaagaag
     5281 ccacctatga tgtcaataga tgatgcttat gaagtgctta atctgcctca aggacaggga
     5341 ccgcatgatg agagcaagat taggaaagct tacttcagac ttgcacaaaa gtacgcagct
     5401 gccaagaatc cagaagggag ggacatgttt gaaaaagtaa ataaagcata tgaattttta
     5461 tgtaccaaat cagcaaaaat agtggatggg ccagatccag agaatataat tttaattcta
     5521 aaaacacaga gcatcctctt caaccgtcat aaagaagatt tacagcctta taaatatgca
     5581 ggatacccca tgcttattcg gactataaca atggaaactt cagatgacct ccttttctca
     5641 aaagaatcac cattgttgcc tgcggctaca gagctagctt tccatactgt caactgttca
     5701 gccctcaatg ctgaagagct cagaagagag aatggactag aggtgttaca agaggcattt
     5761 agtcgctgtg tggctgtctt gactcgtgct agtaaaccaa gtgacatgtc agtacaggtg
     5821 tgtggataca taagtaaatg ctacagtgtg gctgctcagt ttgaggaatg ccgagagaag
     5881 atcacggaaa tgcctagcat catcaaggat ctctgtcggg tactatattt tggcaagagt
     5941 attccccgcg tagctgctct tggggtagaa tgtgtcagtt cttttgctgt ggatttctgg
     6001 ctacagacac acctatttca ggctggaatt ttgtggtatc tccttggttt tctgtttaat
     6061 tatgactaca cactagaaga gagtggcatt cagaaaagtg aagaaacaaa ccagcaggag
     6121 gtagcaaaca gccttgccaa actgagtgtc catgctctga gtcgccttgg agggtatttg
     6181 gctgaagaac aagcaactcc agaaaatcca accataagga aaagcttagc tggcatgctg
     6241 acaccctatg ttgctagaaa acttgctgtg gctagtgtga ctgagatttt gaagatgctt
     6301 aacagcaaca cagaaagtcc atatttgata tggaacaatt ctacaagagc agaattactt
     6361 gaatttcttg aatcccaaca agaaaacatg attaaaaaag gtgattgtga caaaacttat
     6421 ggatcagaat ttgtctacag tgatcatgcc aaagaactta ttgtagggga gatttttgtt
     6481 agggtgtata atgaagttcc tactttccaa ctggaggttc caaaagcatt tgctgcaagt
     6541 ctcttggatt atataggctc gcaggcccaa tacttgcaca cattcatggc catcacacac
     6601 gcggcaaaag tggagtcaga gcaacatgga gatcgcttac cgagagtaga aatggctttg
     6661 gaggctctga gaaatgtcat aaaatacaat ccaggttctg agagtgaatg cattgggcac
     6721 tttaagttga tattttctct tctccgagtt catggagctg gtcaagtgca gcagttggct
     6781 ttagaggttg tgaatatagt gacatctaac caagactgtg tcaacaatat tgctgaatca
     6841 atggttttgt ccagtttatt ggctcttcta cattcattgc catcaagtcg tcagcttgtt
     6901 ctggaaactc tttatgcttt gacatcgagt acaaaaataa tcaaagaagc aatggcaaag
     6961 ggtgctttga tctatttact ggatatgttc tgcaattcaa cacatccaca ggttcgagcc
     7021 caaacagcag aactttttgc caaaatgaca gcagataaac tgataggtcc aaaggttcga
     7081 attacgttaa tgaaatttct accaagcgtt ttcatggatg ctatgagaga caatcctgaa
     7141 gctgctgtac atatttttga aggaactcat gaaaatcctg agttaatttg gaatgataat
     7201 tccagagata aagtgtccac aacagttagg gaaatgatgc tagagcactt taaaaatcag
     7261 caggacaacc ctgaggcaaa ctggaagttg cctgaagatt ttgctgtggt gtttggagaa
     7321 gcagagggtg aacttgctgt tggaggagtc ttcttgagga tctttattgc acaaccagcc
     7381 tgggttctaa gaaagcctag agaatttctt attgccctgt tagaaaaatt aactgagctc
     7441 ctagagaaga acaatcctca tggagaaact ctggaaacct tgacaatggc aacagtgtgt
     7501 ctcttcagcg cacaacctca gctggcagat caggtcccgc cattgggcca tcttcccaaa
     7561 gttatccagg caatgaatca taggaacaat gccattccta agagtgccat tcgggttatc
     7621 catgccttgt ctgaaaatga gctgtgtgtt cgagccatgg catctttaga gaccattggc
     7681 ccactgatga atggaatgaa aaagcgagca gatactgttg gtctagcctg tgaagcaatt
     7741 aatcgaatgt ttcagaagga gcagagtgaa ttagtagcac aagccctgaa agcagatttg
     7801 gttccatacc tcttaaaatt actcgaaggc attggccttg aaaacctgga cagcccagca
     7861 gccactaagg ctcagattgt taaagctctc aaggcaatga ctcgaagttt gcagtatgga
     7921 gaacaggtga atgaaatcct gtgccgttct tcagtctgga gtgccttcaa agatcagaaa
     7981 catgatttgt tcatttgagc ggccgcggat ccaccggatc tagataactg atcataatca
     8041 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     8101 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     8161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat
     8281 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     8341 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     8401 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     8461 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     8521 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     8581 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     8641 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     8701 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8761 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8821 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     8881 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8941 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9001 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9061 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9121 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     9181 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     9241 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     9301 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     9361 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     9421 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     9481 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     9541 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     9601 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     9661 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     9721 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     9781 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     9841 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     9901 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     9961 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
    10021 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
    10081 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
    10141 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
    10201 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
    10261 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
    10321 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
    10381 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
    10441 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
    10501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
    10801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11341 ccatgcat
//
