LOCUS       pREG_SRTF1-246680        3957 bp ds-DNA     circular     08-SEP-2025
DEFINITION  .
ACCESSION   urn.local...q-jmr1mgl
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Imported using the Genbank importer. File name: pREG_CmeR.gb       
            Resistance markers: Ampicillin This file was created using tools   
            provided by Twist Bioscience.
FEATURES             Location/Qualifiers
     misc_feature    15..63
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="ECK12_lpd term"
     misc_feature    70..122
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="P150"
     misc_feature    94..99
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="-35"
     misc_feature    117..122
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="-10"
     misc_feature    128..130
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="TSS"
     ribozyme        148..222
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="riboJ"
     misc_feature    248..306
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="BCD 4.6k"
     CDS             255..305
                     /translation="MKAIFVLKHLNHAPVF*"
                     /label="Translation 255-305"
     primer_bind     complement(281..307)
                     /Sequence="ATTTAAAACACCGGCGCATGATTAAGA"
                     /Tm="62.4"
                     /%GC="37.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="10.8"
                     /created_by="aliaclark-elsayed"
                     /label="AC051"
     unsure          285..897
                     /created_by="aliaclark-elsayed"
                     /modified_by="aliaclark-elsayed"
                     /label="gAC012"
     unsure          285..>307
                     /created_by="aliaclark-elsayed"
                     /label="HA"
     primer_bind     287..326
                     /Sequence="TCATGCGCCGGTGTTTTAAATATGAGCTCGACGGCTGAAC"
                     /Tm="68.2"
                     /%GC="50.0"
                     /Hairpin_Tm="46.6"
                     /Self_Dimer_Tm="15.0"
                     /created_by="aliaclark-elsayed"
                     /label="AC039"
     CDS             308..874
                     /created_by="aliaclark-elsayed"
                     /label="SRTF1"
     primer_bind     complement(853..896)
                     /Sequence="AGTGGCGAGAAGACTAGGATTAGGCATCGTCGTCTGCTAACAGA"
                     /Tm="68.5"
                     /%GC="50.0"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="9.6"
                     /created_by="aliaclark-elsayed"
                     /label="AC040"
     unsure          <875..897
                     /created_by="aliaclark-elsayed"
                     /label="HA"
     misc_feature_   875..307
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="vector_backbone-636-4027"
     primer_bind     875..899
                     /Sequence="TAATCCTAGTCTTCTCGCCACTTTC"
                     /Tm="60.2"
                     /%GC="44.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="aliaclark-elsayed"
                     /label="AC050"
     misc_feature_   1098..1126
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="AmpR_promoter"
     misc_feature_   1168..2028
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="amp"
     misc_feature_   2162..2195
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="FRT_(minimal"
     misc_feature    2244..2284
                     /Original_Bases="TGTAGCATGTAGCATCTTCATAGTTAGACCTTTCATCAAAG
                     "
                     /label="TGTAGCATGTAGCATCTTCATAGTTAGACCTTTCATCAAAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature_   2644..2835
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="ROP"
     misc_feature    2733
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature_   3257..3844
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="colE1_high_copy"
     misc_feature    3297
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3835^3836
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3936..3941
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="BsmBI"
ORIGIN      
        1 gcctctggtt catctccggc aattaaaaaa gcggctaacc acgccgcttt ttttacgtct
       61 gcaggaacgg gctgtcgacc tttgaaaagt tcgtttaccg ctagctcagt cctaggtaca
      121 attacagcca tcgtagaaga cactggcagc tgtcaccgga tgtgctttcc ggtctgatga
      181 gtccgtgagg acgaaacagc ctctacaaat aattttgttt aagggcccaa gttcacttaa
      241 aaaggagatc aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gcgccggtgt
      301 tttaaatatg agctcgacgg ctgaacgtat tcgtccggga cgctctggta ttttggcagc
      361 ggccacccgc ctgtttgcaa ctcacggggt atctggtacg tcgctgcagc agattgccga
      421 cgcaacgggc attacaaaag ccgctgtgta ccaccacttc cctacgaaag aggaggtcgt
      481 cgtcgccgtt cttgcacctg ctttagaagc cattcagggc attgtccgta ccgctggtgc
      541 gcatgaggac ccgcgtgctg caacagaggc tgcaattatc ggacttgcgg accaggcagt
      601 tacgcatcgt caacgctggg cagtcctttt acaggacgcc gccgttgagg agtatgtacg
      661 taataatcct gatcacgatg aattatttac gcgccttcgc ctgcttctga cggggcccga
      721 tcctacaccg ggtacacgtt tgcaggtttc gctgttcctg tctggtttat tgggaccggc
      781 gcaggatcca tcatgcgccg acattgatga tgatgcgtta cgtgcaggca tcgtacgcgc
      841 tggacgccgc cttctgttag cagacgacga tgcctaatcc tagtcttctc gccactttca
      901 gccaaaaaac ttaagaccgc cggtcttgtc cactaccttg cagtaatgcg gtggacagga
      961 tcggcggttt tcttttctct tctcaacacc cttcgcgtca acacttttcc gccaaggaga
     1021 cggttggtca ggttttcggg aggtgtggct ggaagttcct atactttcta gagaatagga
     1081 acttctttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1141 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1201 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1261 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1321 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1381 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     1441 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1501 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1561 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1621 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1681 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     1741 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1801 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1861 aaatctggag ccggtgagcg tggatcgcgc ggtatcattg cagcactggg gccagatggt
     1921 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1981 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaagt tgtgatggcg
     2041 gtaggaatgt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta
     2101 tggggggagt ttagggaaag agcatttgtc atcccgttga atatggctcc cttaacgtga
     2161 ggaagttcct atactttcta gagaatagga acttctacag atggacttgg gttggcggcc
     2221 tagacgtgtg atagactcag tcggcttcga tgtttgagtt gtgtgttttt acatcatatg
     2281 acatcaggag tctgcaaaac gtctgcgacc tgagcaacaa catgaatggt catcggtttc
     2341 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
     2401 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
     2461 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
     2521 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
     2581 ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca
     2641 tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca
     2701 ttaacgcttc tggagaaact caacgagctg gatgcggatg aacaggcaga catctgtgaa
     2761 tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac
     2821 ggtgaaaacc tctgacacat gcagctcccg cagacggtca cagcttgtct gtaagcggat
     2881 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     2941 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     3001 agcagattgt actgagagtg caccggtgtg aaataccgca cagatgcgta aggagaaaat
     3061 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3181 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3241 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaattgac
     3301 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3361 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3421 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3481 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3541 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3601 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3661 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3721 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3781 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaggatc
     3841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3901 ttaaggccct ctccaagacc gagccatcaa caaagcgtct cgctgaggtt tcatgga
//
