LOCUS       pSELIS_SRTF1-246681        5984 bp ds-DNA     circular     08-SEP-2025
DEFINITION  .
ACCESSION   urn.local...1363478693202.151
VERSION     urn.local...1363478693202.151
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Imported using the Genbank importer. File name: og_pramz.gb.
FEATURES             Location/Qualifiers
     misc_feature    complement(36..875)
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="ApEinfo_revcolor: #faac61"
                     /label="p15A"
     misc_feature    415
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    503
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1108..1133
                     /Original_Bases="CCAACTTTGGCGAAAATGAGACGTGA"
                     /label="CCAACTTTGGCGAAAATGAGACGTGA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1135..1137
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1237..1896
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="ApEinfo_revcolor: #b7e6d7"
                     /label="CAT"
     misc_feature    1280
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     terminator      complement(2117..2164)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="ApEinfo_revcolor: #b4abac"
                     /label="LuxI terminator"
     misc_feature    complement(2168..2542)
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9"
                     /label="EZeo"
     CDS             complement(2168..2542)
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /label="Translation 2539-2165"
     CDS             complement(2168..2542)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
     misc_feature    complement(2548..2568)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
                     /label="Med-Strong RBS"
     Promoter        2595
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
                     /label="+1"
     Promoter        complement(2596..2644)
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="ApEinfo_revcolor: #c6c9d1"
                     /label="Lambda PR"
     terminator      2670..2703
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
                     /label="Bidirectional Terminator"
     unsure          2707..3739
                     /created_by="aliaclark-elsayed"
                     /modified_by="aliaclark-elsayed"
                     /label="gAC011"
     primer_bind     2707..2734
                     /Sequence="CAGGGCTATAAGGTAGATCCGGGAGCTG"
                     /Tm="67.3"
                     /%GC="57.1"
                     /Hairpin_Tm="42.0"
                     /Self_Dimer_Tm="24.4"
                     /created_by="aliaclark-elsayed"
                     /label="homology"
     primer_bind     complement(2715..2734)
                     /Sequence="CAGCTCCCGGATCTACCTTA"
                     /Tm="58.0"
                     /%GC="55.0"
                     /Hairpin_Tm="36.3"
                     /Self_Dimer_Tm="0.7"
                     /created_by="aliaclark-elsayed"
                     /label="AC010"
     promoter        2735..2769
                     /created_by="aliaclark-elsayed"
                     /label="J23119"
     misc_feature    2740..2791
                     /Original_Bases="GGCGTATCTTTGCTTTCTATAATGAGTGCTTACTCACTCAT
                     "
                     /label="GGCGTATCTTTGCTTTCTATAATGAGTGCTTACTCACTCAT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(2748..2769)
                     /Sequence="GCTAGCATTATACCTAGGACTG"
                     /Tm="55.3"
                     /%GC="45.5"
                     /Hairpin_Tm="33.3"
                     /Self_Dimer_Tm="18.0"
                     /created_by="aliaclark-elsayed"
                     /label="AC020"
     primer_bind     2750..2817
                     /Sequence="GTCCTAGGTATAATGCTAGCTAACTAGCCGTTCGGCTAGTTAAATCA
                     AGTACTAGAGAAGGAGATCTA"
                     /Tm="76.6"
                     /%GC="41.2"
                     /created_by="aliaclark-elsayed"
                     /label="AC024"
     primer_bind     2750..2769
                     /Sequence="GTCCTAGGTATAATGCTAGC"
                     /Tm="52.1"
                     /%GC="45.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.1"
                     /created_by="aliaclark-elsayed"
                     /label="Homology"
     protein_bind    2770..2791
                     /created_by="aliaclark-elsayed"
                     /label="SRTF1_bind"
     primer_bind     2792..2817
                     /created_by="aliaclark-elsayed"
                     /Sequence="AATCAAGTACTAGAGAAGGAGATCTA"
                     /Tm="56.2"
                     /%GC="34.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="aliaclark-elsayed"
                     /label="Anneal"
     misc_feature    2807..2813
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
                     /label="RBS"
     CDS             2820..3530
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /label="Translation 2806-3516"
     CDS             2820..3530
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
                     /label="cI Repressor"
     CDS             2820..3530
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETF*"
     terminator      3557..3619
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="ApEinfo_revcolor: #d6b295"
                     /label="L3S2P00 terminator"
     primer_bind     complement(3636..3655)
                     /created_by="aliaclark-elsayed"
                     /Sequence="TTTCAAAGGTCGACAGCCCA"
                     /Tm="59.8"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="18.9"
                     /modified_by="aliaclark-elsayed"
                     /label="AC012"
     promoter        3656..3690
                     /created_by="aliaclark-elsayed"
                     /label="J23119"
     misc_feature    3661..3690
                     /Original_Bases="GGCGTATCTTTGCTTTCTATAATGAGTGCTTACTCACTCA"
                     /label="GGCGTATCTTTGCTTTCTATAATGAGTGCTTACTCACTCA"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(3669..3690)
                     /Sequence="GCTAGCATTATACCTAGGACTG"
                     /Tm="55.3"
                     /%GC="45.5"
                     /Hairpin_Tm="33.3"
                     /Self_Dimer_Tm="18.0"
                     /created_by="aliaclark-elsayed"
                     /label="AC022"
     primer_bind     3671..3739
                     /Sequence="GTCCTAGGTATAATGCTAGCTAACTAGCCGTTCGGCTAGTTAGGGTT
                     TGATTTAATAAGGAGGTATACA"
                     /Tm="76.5"
                     /%GC="40.6"
                     /created_by="aliaclark-elsayed"
                     /label="AC023"
     primer_bind     3671..3690
                     /Sequence="GTCCTAGGTATAATGCTAGC"
                     /Tm="52.1"
                     /%GC="45.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.1"
                     /created_by="aliaclark-elsayed"
                     /label="Homology"
     protein_bind    3691..3712
                     /created_by="aliaclark-elsayed"
                     /label="SRTF1_bind"
     misc_feature    3693..3712
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3713..3734
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="ApEinfo_revcolor: #f58a5e"
                     /label="Strong RBS"
     primer_bind     3713..3739
                     /created_by="aliaclark-elsayed"
                     /Sequence="GGGTTTGATTTAATAAGGAGGTATACA"
                     /Tm="57.1"
                     /%GC="33.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.0"
                     /modified_by="aliaclark-elsayed"
                     /label="Annealing"
     primer_bind     3713..3739
                     /Sequence="GGGTTTGATTTAATAAGGAGGTATACA"
                     /Tm="57.1"
                     /%GC="33.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.0"
                     /created_by="aliaclark-elsayed"
                     /label="homology"
     CDS             3741..4484
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b1ff67"
                     /label="sfGFP"
     CDS             3741..4484
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFK
                     DDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKN
                     GIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDH
                     MVLLEFVTAAGITHGMDELYKLEHHHHHH*"
     terminator      4488..4616
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="ApEinfo_revcolor: #9eafd2"
                     /label="rrnb.TE Tandem Terminator"
     misc_feature    4731..5425
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
                     /label="LacI"
     CDS             4731..5425
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /transl_table=11
                     /Original_Translation="VVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSA
                     KTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQL
                     GASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFL
                     DVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLT
                     RNQIQPIAEREGDWSAMSGFQQ"
                     /label="test"
     misc_feature    4852
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5302
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5647
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 tttgaagaga taaattgcac tgaaatctag aaatatttta tctgattaat aagatgatct
       61 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
      121 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
      181 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
      241 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
      301 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
      361 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgttaggcg
      421 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
      481 caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttat agtcctgtcg
      541 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
      601 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
      661 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
      721 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
      781 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
      841 ccaacatagt aagccagtat acactccgct agcgctgatg tccggcggtg cttttgccgt
      901 tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg
      961 gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc acccgacgca
     1021 ctttgcgccg aataaatacc tgtgacggaa gatcacttcg cannataaat aaatcctggt
     1081 gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga cgttgatcgg
     1141 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
     1201 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1261 accaccgttg atatatcccg atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1321 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1381 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1441 gctcatccgg agttccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1501 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1561 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1621 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     1681 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     1741 cccgttttca ctatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     1801 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     1861 caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     1921 ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     1981 tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg
     2041 tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa
     2101 atgcctgagg tttcagaaat aataaaaaag ccggattaat aatctggctt tttatattct
     2161 ctctttatta atcttgttct tcagcaacaa aatgaacgca attaccagct ggatctctca
     2221 aagcaaattc tctaccccaa ggttgttcac caatttcggt catagctgga ccagaagcat
     2281 ctctaaaatt ggtagaaaca acttcggacc attcggcata taattcatcc aaacctctaa
     2341 cccaaaccca agccaaagta ttatctggaa caacttgatc ttgaacggcg gaaatgaaca
     2401 aggtaacatc atctctaaca acaccagcga aatcatcttc aacgaaatct ctggagaaac
     2461 ccaatctatc agtccaaaat tcaacagcac cagcaacatc tctagcggtc aaaactggaa
     2521 cagcagatgt caatttagac atatgtcaac ctcctccaat aaccgtcact gctgacgact
     2581 ctaccattat cacttgcaac cattatcacc gccagaggta aaatagtcaa cacgcacggt
     2641 gttagtcttt tcgccggtgt atttcttgca aaaaaaaacc ccgccgaagc ggggtttttt
     2701 tttttacagg gctataaggt agatccggga gctgttgaca gctagctcag tcctaggtat
     2761 aatgctagct aactagccgt tcggctagtt aaatcaagta ctagagaagg agatctagaa
     2821 tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag
     2881 caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga
     2941 tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt
     3001 ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa
     3061 tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg
     3121 agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa
     3181 cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg
     3241 cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct
     3301 ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct
     3361 gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc
     3421 aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt
     3481 ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgttttaa cgataatgtc
     3541 gggcaatcag gtgcgactcg gtaccaaatt ccagaaaaga ggggagcggg aaaccgctcc
     3601 ccttttttcg ttttggtccc aatctatcga ttgtatgggc tgtcgacctt tgaaattgac
     3661 agctagctca gtcctaggta taatgctagc taactagccg ttcggctagt tagggtttga
     3721 tttaataagg aggtatacat atggcatcca agggcgagga gctctttact ggcgtagtac
     3781 caattctcgt agagctcgat ggcgatgtaa atggccataa gttttccgta cgcggcgagg
     3841 gcgagggcga tgcaactaac ggcaagctca ctctcaagtt tatttgtact actggcaagc
     3901 tcccagtacc atggccaact ctcgtaacta ctctgaccta tggcgtacaa tgtttttccc
     3961 gctatccaga tcacatgaag caacatgatt tttttaagtc cgcaatgcca gagggctatg
     4021 tacaagagcg cactattagc tttaaggatg atggcaccta taagactcgc gcagaggtaa
     4081 agtttgaggg cgatactctc gtaaatcgca ttgagctcaa gggcattgat tttaaggagg
     4141 atggcaatat tctcggccat aagctggagt ataatttcaa ttcccataat gtatatatta
     4201 ccgcagataa gcaaaagaat ggcattaagg cgaattttaa gattcgccat aatgtggagg
     4261 atggctccgt acaactcgca gatcattatc aacaaaatac tccaattggc gatggcccag
     4321 tactcctccc agataatcat tatctctcca ctcaatccgt gctctccaaa gatccaaatg
     4381 agaagcgcga tcacatggta ctcctggagt ttgtaactgc agcaggcatt actcatggca
     4441 tggatgagct ctataagctc gagcaccacc accaccacca ctaataacca ggcatcaaat
     4501 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     4561 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataattt
     4621 cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca
     4681 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg
     4741 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     4801 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gcggaagcgg
     4861 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     4921 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     4981 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     5041 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     5101 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     5161 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     5221 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     5281 aaatcgcgct gttagcgggc ctattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     5341 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     5401 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     5461 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     5521 ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa gacagctcat
     5581 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     5641 tggaccactt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     5701 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     5761 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5821 gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg
     5881 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactaacatg
     5941 agaattacaa cttatatcgt atggggctga cttcaggtgc taca
//
