LOCUS       EKAREN5-puro(pLV       10179 bp    DNA     circular SYN 24-JUN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    44..644
                     /label=IRES(MMlab)
                     /note="color: #a6acb3"
     misc_feature    73..625
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     primer_bind     complement(136..154)
                     /label=IRES-R-seq 1292
                     /note="color: 黒; sequence: CCAAAAGACGGCAATATGG; added: 
                     2021-07-29"
     primer_bind     complement(136..154)
                     /label=IRES-R-seq 13102
                     /note="color: 黒; sequence: CCAAAAGACGGCAATATGG; added: 
                     2021-07-29"
     primer_bind     complement(445..467)
                     /label=sIRES-R  8992
                     /note="color: 黒; sequence: gttgaatacgcttgaggagagcc; added: 
                     2021-07-29"
     regulatory      646..651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             652..1251
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             2140..2373
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    2445..2566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      2585..2720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(2753..2771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(2781..2797)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      2942..3397
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        3426..3530
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(3531..3599,3600..4391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3531 .. 3599 / #ccffcc / signal sequence
                        2: 3600 .. 4391 / #ccffcc
                     Cleavage site after base 3599"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4562..5150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    5438..5459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        join(5474..5479,5480..5497,5498..5504)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 5474 .. 5479 / #ffffff / -35
                        2: 5480 .. 5497 / #ffffff
                        3: 5498 .. 5504 / #ffffff / -10"
     protein_bind    5512..5528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     5536..5552
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        5573..5591
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             5798..6055
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    6099..6224
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    6717..6950
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             7134..7178
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7440..7557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        7605..8767
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          7836..8758
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     8680..8700
                     /label=pEF1a-Fwd
                     /note="color: 黒; sequence: ATTTGCCCTTTTTGAGTTTGG; added: 
                     2022-08-08"
     primer_bind     8699..8722
                     /label=pCSII-seq-Fw 6535/9313
                     /note="color: 黒; sequence: GGATCTTGGTTCATTCTCAAGCCT; added:
                     2021-07-29"
     primer_bind     8709..8732
                     /label=CSII-seq-F 5770/5868
                     /note="color: 黒; sequence: tcattctcaagcctcagacagtgg; added:
                     2021-07-29"
     primer_bind     8721..8742
                     /label=seq_PCSIIvector_f 8806
                     /note="color: 黒; sequence: CTCAGACAGTGGTTCAAAGTTT; added: 
                     2021-07-29"
     primer_bind     8739..8759
                     /label=EF-BOS-F 10232
                     /note="color: 黒; sequence: gtttttttcttccatttcagg; added: 
                     2021-07-29"
     primer_bind     8822..8840
                     /label=Des-ENCY1-Fwd-sub 13602
                     /note="color: 黒; sequence: ctcgagatggcggacgagg; added: 
                     2021-05-14"
     primer_bind     8823..8840
                     /label=Inf-F2-4238NES 6457
                     /note="color: 黒; sequence: TCGAGATGGCGGACGAGG; added: 
                     2021-07-29"
     misc_feature    8825..8989
                     /label=WW domain
                     /note="color: #a6acb3"
     primer_bind     8825..8849
                     /label=6082NES_WWsubstrate_inf_F 9066
                     /note="color: 黒; sequence: GAGATGGCGGACGAGGAGAAGCTGC; 
                     added: 2021-07-29"
     primer_bind     8896..8920
                     /label=Des-ENCY1-Fwd
                     /note="color: 黒; sequence: ctacttcaaccacatcactaacgcc; 
                     added: 2021-05-17"
     primer_bind     8947..8968
                     /label=3903-Fw 5066
                     /note="color: 黒; sequence: CAGCAGCAGTGGTGGCAAAAAC; added: 
                     2021-07-29"
     primer_bind     8962..8985
                     /label=WW_seq_F 4583
                     /note="color: 黒; sequence: CAAAAACGGGCAGGGGGAGCCTGC; added:
                     2021-07-29"
     misc_feature    9002..9337
                     /label=EV linker
                     /note="color: #00ff00"
     misc_feature    9350..9403
                     /label=ERK substrate
                     /note="color: #a6acb3"
     primer_bind     9370..9405
                     /label=Ins-ENCY2-Fwd 13603
                     /note="color: 黒; sequence: 
                     AGTGGATCCAGCATGGCTGTCATTCCAATTTCCGGG; added: 2021-07-29"
     misc_feature    9413..10123
                     /label=mTurquoise2
                     /note="color: #00ffff"
     primer_bind     complement(9458..9474)
                     /label=MMlab203
                     /note="color: 黒; sequence: CGTCGCCGTCCAGCTCGACCAG; added: 
                     2022-10-11"
     primer_bind     complement(9582..9601)
                     /label=mTurquoise2_Rev1 13736
                     /note="color: 黒; sequence: tgtcacgagtgtaggccaag; added: 
                     2021-07-02"
     primer_bind     complement(9609..9628)
                     /label=mTurquoise2_Rev2 13737
                     /note="color: 黒; sequence: aaaacactgcactccccaag; added: 
                     2021-07-02"
     primer_bind     complement(10025..10047)
                     /label=MMlab2228
                     /note="color: 黒; sequence: CGTTGGGGTCTTTGAACAGGGCGGACTG; 
                     added: 2022-10-11"
     primer_bind     10069..10098
                     /label=MMlab6039
                     /note="color: 黒; sequence: GGTCCTGCTGGAGTTCCTGACCGCCGCCGG; 
                     added: 2022-10-11"
     CDS             10130..10150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
ORIGIN
        1 cggtcgttaa cattaagcta gcctagtgcc atttgttcag tggcccggga attccgcccc
       61 tctccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
      121 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
      181 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
      241 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
      301 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
      361 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
      421 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
      481 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
      541 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
      601 tcctttgaaa aacacgatga taatatggat atggccacaa cggtcgccac catgaccgag
      661 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
      721 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
      781 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
      841 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
      901 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
      961 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     1021 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     1081 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     1141 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     1201 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     1261 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatgtat tcaatctaag
     1321 caggctttca ctttctcgcc aacttacaag gcctttctgt gtaaacaata cctgaacctt
     1381 taccccgttg cccggcaacg gccaggtctg tgccaagtgt ttgctgacgc aacccccact
     1441 ggctggggct tggtcatggg ccatcagcgc atgcgtggaa ccttttcggc tcctctgccg
     1501 atccatactg cggaactcct agccgcttgt tttgctcgca gcaggtctgg agcaaacatt
     1561 atcgggactg ataactctgt tgtcctatcc cgcaaatata catcgtttcc atggctgcta
     1621 ggctgtgctg ccaactggat cctgcgcggg acgtcctttg tttacgtccc gtcggcgctg
     1681 aatcctgcgg acgacccttc tcggggtcgc ttgggactct ctcgtcccct tctccgtctg
     1741 ccgttccgac cgaccacggg gcgcacctct ctttacgcgg actccccgtc tgtgccttct
     1801 catctgccgg accgtgtgca cttcgcttca cctctgcacg tcgcatggag accaccgtga
     1861 acgcccacca aatattgccc aaggtcttac ataagaggac tcttggactc tcagcaatgt
     1921 caacgaccga ccttgaggca tacttcaaag actgtttgtt taaagactgg gaggagttgg
     1981 gggaggagat taggttaaag gtctttgtac taggaggctg taggcataaa ttggtctgcg
     2041 caccagcacc atgtatcact agagcggggt acctttaaga ccaatgactt acaaggcagc
     2101 tgtagatctt agccactttt taaaagaaaa ggggggactt ggaagggcta attcactccc
     2161 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     2221 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2281 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2341 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     2401 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     2461 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     2581 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2641 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     2701 aggaggcttt tttggaggcc taggcttttg cgtcgagacg tacccaattc gccctatagt
     2761 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2821 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2881 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     2941 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3001 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3061 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3121 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3181 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3241 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     3301 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3361 aacgcgaatt ttaacaaaat attaacgttt acaatttccc aggtggcact tttcggggaa
     3421 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3481 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3541 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3601 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3661 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3721 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3781 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3841 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3901 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3961 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4021 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4081 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4141 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4201 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4261 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4321 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4381 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4441 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4501 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4561 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4621 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4681 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4741 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4801 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4861 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4921 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4981 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5041 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5101 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5161 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5221 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5281 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5341 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5401 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5461 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5521 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     5581 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     5641 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     5701 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     5761 gacgggtctg acatggattg gacgaaccac tgaattggag gcgtggcctg ggcgggactg
     5821 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     5881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5941 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     6061 gcccgaacag ggacctgaaa gcgaaaggga aaccagagct ctctcgacgc aggactcggc
     6121 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     6181 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     6241 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     6301 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     6361 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     6421 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     6481 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     6541 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     6601 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     6661 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     6721 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc ctcaatgacg
     6781 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     6841 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     6901 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     6961 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     7021 aaatctctgg aacagattgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7081 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7141 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7201 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7261 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7321 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     7381 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt
     7441 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     7501 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
     7561 gatcatatga ctagagcggc cttacgacat tgattattga ctagggctcc ggtgcccgtc
     7621 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     7681 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     7741 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     7801 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     7861 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctcc
     7921 agtacgtgat tcttgatccc gagctggagc caggggcggg ccttgcgctt taggagcccc
     7981 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     8041 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     8101 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaggatct
     8161 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     8221 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     8281 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     8341 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     8401 ccctgctcca gggggctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     8461 acccacacaa aggaaagggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     8521 gtaccgggcg ccgtccaggc acctcgatta gttctggagc ttttggagta cgtcgtcttt
     8581 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     8641 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     8701 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     8761 gtcgtgatta attaaacgcg taccgatagc ttcgaattct gcagtcgacg atatcactag
     8821 tctcgagatg gcggacgagg agaagctgcc gcccggctgg gagaagcgca tgagccgcag
     8881 ctcaggccga gtgtactact tcaaccacat cactaacgcc agccagtggg agcggcccag
     8941 cggcaacagc agcagtggtg gcaaaaacgg gcagggggag cctgccaggg gtaccagtgc
     9001 tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggttccgg
     9061 cagtgctggt ggtagtgctg gtggtagtac cagtgctggt ggtagtgctg gtggtagtgc
     9121 tggtggtagt gctggtggta gtgctggtgg ttccggcagt gctggtggta gtgctggtgg
     9181 tagtaccagt gctggtggta gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc
     9241 tggtggttcc ggcagtgctg gtggtagtgc tggtggtagt accagtgctg gtggtagtgc
     9301 tggtggtagt gctggtggta gtgctggtgg tagtgctggt ggttccggac cagatgtccc
     9361 tagaactcca gtggatccag catggctgtc attccaattt ccgggcggcc gcatggtgag
     9421 taaaggggaa gaactgttta ctggcgtggt gccaattctg gtcgaactgg atggagatgt
     9481 aaatgggcat aaatttagtg tgtctggaga aggggaagga gacgctacat atgggaaact
     9541 gacactgaaa tttatttgta ctactggaaa actgcctgtg ccttggccta cactcgtgac
     9601 aaccctgtct tggggagtgc agtgttttgc ccggtatcct gatcatatga aacagcatga
     9661 ttttttcaaa tctgccatgc ctgagggata tgtccaggaa cggacaattt tctttaaaga
     9721 tgatgggaat tataaaacac gggccgaagt gaaatttgaa ggggatacac tggtgaatcg
     9781 gattgaactg aaaggaattg attttaaaga agatgggaat attctgggcc ataaactgga
     9841 atataattat ttcagcgata atgtctacat tacagccgat aaacagaaga atgggattaa
     9901 agccaatttt aaaattcggc ataatattga agatggaggg gtgcagctcg ccgatcatta
     9961 tcagcagaat acacctattg gggatgggcc tgtgctgctg cctgataatc attatctgag
    10021 cacacagtct gccctgagca aggaccctaa tgaaaagcgg gaccatatgg tcctgctgga
    10081 atttctgact gccgccggca ttacactcgg gatggatgaa ctgtctagac ctaagaagaa
    10141 gcgtaaggtg taagtcgacg gtcgagtcga gacgctagc
//
