LOCUS       EKAREN5-puro(pLV       10986 bp    DNA     circular SYN 01-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Toru Hiratsuka
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    77..194
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        222..1383
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          452..1374
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     CDS             join(1465..1467,1468..1470,1471..2178)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1465 .. 1467 / #e1ff2f
                        2: 1468 .. 1470 / #e1ff2f / 1a
                        3: 1471 .. 2178 / #e1ff2f"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     WWdomain        2182..2346
                     /label=WW domain
                     /note="color: #ff9900"
     misc_feature    2348..2705
                     /label=EV linker
                     /note="color: #00ff00"
     CDS             2707..2760
                     /label=ERK substrate
                     /note="color: #ff00ff; direction: RIGHT"
     misc_feature    2770..3486
                     /label=mTurquioise_GL
                     /note="color: #00ffff"
     CDS             3487..3507
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    3715..4267
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     CDS             4294..4893
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             5785..6018
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             5838..6018
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    6090..6211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6251..6386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(6407..6425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6435..6451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      6593..7048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7074..7178
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(7179..7247,7248..8039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7179 .. 7247 / #ccffcc / signal sequence
                        2: 7248 .. 8039 / #ccffcc
                     Cleavage site after base 7247"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8210..8798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    9086..9107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        join(9122..9127,9128..9145,9146..9152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9122 .. 9127 / #ffffff / -35
                        2: 9128 .. 9145 / #ffffff
                        3: 9146 .. 9152 / #ffffff / -10"
     protein_bind    9160..9176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9184..9200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        9221..9239
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        9267..9493
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             9494..9674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    9721..9846
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    10339..10572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             10757..10801
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
       61 atctcgacgg tatcggtttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      121 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      181 aaattcaaaa ttttatcgat catatgacta gagcggcctt aggctccggt gcgcgtcagt
      241 gggcagagcg cacatcgccc acagtccccg agaagttggg ggaggggtcg gcaattgaac
      301 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      361 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      421 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      481 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctccagta
      541 cgtgattctt gatcccgagc tggagccagg ggcgggcctt gcgctttagg agccccttcg
      601 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
      661 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
      721 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca ggatctgcac
      781 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
      841 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
      901 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
      961 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     1021 gctccagggg gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     1081 acacaaagga aaggggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     1141 cgggcgccgt ccaggcacct cgattagttc tggagctttt ggagtacgtc gtctttaggt
     1201 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     1261 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     1321 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     1381 tgattaatta aacgcgtacc gatagcttcg aattctgcag tcgacgatat cactagtctc
     1441 gagcattttg gcaaagaatt cggcatggtg agcaagggcg aggagctgtt caccggggtg
     1501 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1561 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agcttctatg caccaccggc
     1621 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
     1681 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1741 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1801 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1861 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1921 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     1981 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2041 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgtt caaagacccc
     2101 aacgagaagc gcgatcacat ggtcctgctg gagttcctga ccgccgccgg gatcactgag
     2161 ggcatgaacg agctgtacct cgagatggcg gacgaggaga agctgccgcc cggctgggag
     2221 aagcgcatga gccgcagctc aggccgagtg tactacttca accacatcac taacgccagc
     2281 cagtgggagc ggcccagcgg caacagcagc agtggtggca aaaacgggca gggggagcct
     2341 gccaggggta ccagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt
     2401 agtgctggtg gttccggcag tgctggtggt agtgctggtg gtagtaccag tgctggtggt
     2461 agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggttc cggcagtgct
     2521 ggtggtagtg ctggtggtag taccagtgct ggtggtagtg ctggtggtag tgctggtggt
     2581 agtgctggtg gtagtgctgg tggttccggc agtgctggtg gtagtgctgg tggtagtacc
     2641 agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggtag tgctggtggt
     2701 tccggaccag atgtccctag aactccagtg gatccagcat ggctgtcatt ccaatttccg
     2761 ggcggccgca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2821 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2881 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2941 tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg ctaccccgac
     3001 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3061 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3121 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3181 ctggggcaca agctggagta caactacatc agcgggaacg tctatatcac cgccgacaag
     3241 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     3301 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3361 gacaaccact acctgagcac ccagtccgcc ttaagcaaag accccaacga gaagcgcgat
     3421 cacatggtcc tgctggagtt cttgaccgcc gccgggatca ctctcggcat ggacgagctg
     3481 tctagaccta agaagaagcg taaggtgtaa gtcgacggtc gagtcgagaa gctagcctag
     3541 tgccatttgt tcagtggttc gtagggcttt cccccactgt ttggctttca gttatatgga
     3601 tgatgtggta ttgggggcca agtctgtaca gcatcttgag tcccttttta ccgctgttac
     3661 caattttctt ttgtctttgg gtaaacccgg gaattccgcc cctctccccc cctaacgtta
     3721 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     3781 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     3841 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     3901 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     3961 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     4021 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     4081 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     4141 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     4201 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     4261 gataatatgg atatggccac aacggtcgcc accatgaccg agtacaagcc cacggtgcgc
     4321 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     4381 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     4441 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     4501 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     4561 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     4621 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     4681 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     4741 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     4801 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     4861 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     4921 accgaaagga gcgcacgacc ccatgcatgt attcaatcta agcaggcttt cactttctcg
     4981 ccaacttaca aggcctttct gtgtaaacaa tacctgaacc tttaccccgt tgcccggcaa
     5041 cggccaggtc tgtgccaagt gtttgctgac gcaaccccca ctggctgggg cttggtcatg
     5101 ggccatcagc gcatgcgtgg aaccttttcg gctcctctgc cgatccatac tgcggaactc
     5161 ctagccgctt gttttgctcg cagcaggtct ggagcaaaca ttatcgggac tgataactct
     5221 gttgtcctat cccgcaaata tacatcgttt ccatggctgc taggctgtgc tgccaactgg
     5281 atcctgcgcg ggacgtcctt tgtttacgtc ccgtcggcgc tgaatcctgc ggacgaccct
     5341 tctcggggtc gcttgggact ctctcgtccc cttctccgtc tgccgttccg accgaccacg
     5401 gggcgcacct ctctttacgc ggactccccg tctgtgcctt ctcatctgcc ggaccgtgtg
     5461 cacttcgctt cacctctgca cgtcgcatgg agaccaccgt gaacgcccac caaatattgc
     5521 ccaaggtctt acataagagg actcttggac tctcagcaat gtcaacgacc gaccttgagg
     5581 catacttcaa agactgtttg tttaaagact gggaggagtt gggggaggag attaggttaa
     5641 aggtctttgt actaggaggc tgtaggcata aattggtctg cgcaccagca ccatggcgca
     5701 atcactagag cggggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5761 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5821 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5881 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5941 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6001 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6061 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6121 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6241 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6301 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6361 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6421 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6481 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6541 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6601 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6661 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6721 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6781 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6841 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6901 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6961 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7021 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7081 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7141 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7201 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7261 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7321 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7381 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7441 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7501 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7561 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7621 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7681 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7741 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7801 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7861 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7921 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7981 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8041 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8101 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8161 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8221 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8281 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8341 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8401 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8461 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8521 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8581 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8641 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8701 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8761 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8821 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8881 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8941 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9001 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9061 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9121 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9181 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9241 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     9301 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9361 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     9421 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     9481 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9541 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9601 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9661 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     9721 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     9781 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     9841 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     9901 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     9961 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10021 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10081 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10141 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
    10201 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
    10261 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
    10321 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
    10381 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
    10441 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
    10501 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
    10561 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
    10621 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
    10681 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10741 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10801 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10861 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10921 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    10981 gcccga
//
