LOCUS       Exported               17363 bp ds-DNA     circular SYN 01-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 2, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..17363
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        274..708
                     /label=35Sen
     promoter        709..1171
                     /label=CmYLCV
     primer_bind     1095..1114
                     /label=CmYLCV-EndF
     promoter        1180..1267
                     /label=U6 (88-bp)
     tRNA            1275..1343
                     /label=tOsGly (2-bp deletion, CA)
     misc_feature    1344..1363
                     /label=stuffer seq
     misc_RNA        1364..1439
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    1440..1446
                     /label=polyT
     misc_feature    1447..1514
                     /label=HDV
     terminator      1523..1793
                     /label=HSP18.2t
     primer_bind     complement(1594..1619)
                     /label=HSP-R
     misc_feature    1807..3797
                     /label=Ubi promoter
     CDS             3813..8084
                     /codon_start=1
                     /label=zCas9
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYS
                     IGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLK
                     RTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD
                     EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDK
                     LFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
                     LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
                     GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGEL
                     HAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
                     EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKP
                     AFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHD
                     LLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
                     GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
                     QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINR
                     LSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKL
                     ITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
                     REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
                     YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGE
                     TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWD
                     PKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKG
                     YKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKL
                     KGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
                     QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS
                     QLGGDKRPAATKKAGQAKKKK"
     misc_feature    8089..8723
                     /label=E9ter
     primer_bind     complement(8738..8754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9043..9719
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             9786..10811
                     /codon_start=1
                     /label=Hyg
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10852..11026
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11103..11128
                     /label=LB
     CDS             11553..12347
                     /codon_start=1
                     /label=KmR
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12638..12918
                     /direction=RIGHT
                     /label=pBR322 origin
     misc_feature    complement(13692..13886)
                     /label=pVS1 ori
     misc_feature    13892..13897
                     /label=Muatated BsaI
     CDS             complement(13952..15025)
                     /codon_start=1
                     /label=RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15454..16083)
                     /codon_start=1
                     /label=StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttatggagt caaagattca aatagaggac
      301 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
      361 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
      421 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      481 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      541 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      601 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      661 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgattg gcagacatac
      721 tgtcccacaa atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg cgtctgacaa
      781 aggttaggtc ggctgccttt aatcaatacc aaagtggtcc ctaccacgat ggaaaaactg
      841 tgcagtcggt ttggcttttt ctgacgaaca aataagattc gtggccgaca ggtgggggtc
      901 caccatgtga aggcatcttc agactccaat aatggagcaa tgacgtaagg gcttacgaaa
      961 taagtaaggg tagtttggga aatgtccact cacccgtcag tctataaata cttagcccct
     1021 ccctcattgt taagggagca aaatctcaga gagatagtcc tagagagaga aagagagcaa
     1081 gtagcctaga agtagtcaag gcggcgaagt attcaggcac gtggccagga agaagaaaag
     1141 ccaagacgac gaaaacaggt aagagctaag catctagata agttgaaaac aatcttcaaa
     1201 agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta gagtcgaagt
     1261 agtgattgaa caaagcacca gtggtctagt ggtagaatag taccctgcca cggtacagac
     1321 ccgggttcga ttcccggctg gtgtgagacc ctgcagggtc tctgttttag agctagaaat
     1381 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     1441 ttttttggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac atgcttcggc
     1501 atggcgaatg ggactttttt ttgatatctc cggggctaat tgaatatgaa gatgaagatg
     1561 aaatatttgg tgtgtcaaat aaaaagctgg tgtgcttaag tttgtgtttt tttcttggct
     1621 tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat ctcattataa
     1681 tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca
     1741 tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat aagaagcttg
     1801 catgcctgca gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat
     1861 gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt
     1921 atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct atagtactac
     1981 aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca
     2041 attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc
     2101 tttttttttg caaatagctt cacctatata atacttcatc cattttatta gtacatccat
     2161 ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct attttattct
     2221 attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt
     2281 agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt
     2341 aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc
     2401 gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa
     2461 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc
     2521 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc
     2581 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc
     2641 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
     2701 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
     2761 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc cccccctctc
     2821 taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc
     2881 atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg
     2941 cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc
     3001 ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt
     3061 gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg
     3121 ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc
     3181 gttctagatc ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc
     3241 tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg
     3301 atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt
     3361 tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg
     3421 agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg
     3481 tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat
     3541 acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat
     3601 atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat aattattttg
     3661 atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg
     3721 ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt
     3781 tggtgttact tctgcagccc taggcctact agatggatta caaggaccac gacggggatt
     3841 acaaggacca cgacattgat tacaaggatg atgatgacaa gatggctccg aagaagaaga
     3901 ggaaggttgg catccacggg gtgccagctg ctgacaagaa gtactcgatc ggcctcgata
     3961 ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga
     4021 agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc
     4081 tcctgttcga cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc
     4141 ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg
     4201 cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata
     4261 agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga
     4321 agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc
     4381 tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg
     4441 agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga
     4501 cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta
     4561 tcctgtccgc taggctctcg aagtctcggc gcctcgagaa cctgatcgcc cagctgccgg
     4621 gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca
     4681 acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat
     4741 acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc
     4801 tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg
     4861 agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg
     4921 atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct
     4981 tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg
     5041 agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg
     5101 tgaagctcaa tcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc
     5161 cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc
     5221 ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact
     5281 acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg
     5341 agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt
     5401 tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc
     5461 actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga
     5521 ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc
     5581 tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga
     5641 agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc
     5701 tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg
     5761 agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga
     5821 tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc
     5881 tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc
     5941 gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc
     6001 gcaatttcat gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg
     6061 ctcaggtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc
     6121 cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca
     6181 tgggcaggca caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc
     6241 agaagggcca gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc
     6301 tggggtccca gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc
     6361 tctacctgta ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta
     6421 acaggctcag cgattacgac gtcgatcata tcgttccaca gtcattcctg aaggatgact
     6481 ccattgacaa caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc
     6541 cttcagagga ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc
     6601 tgatcacgca gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc
     6661 tggacaaggc gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg
     6721 ttgcgcagat tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc
     6781 gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc
     6841 agttctacaa ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg
     6901 cggtggtcgg cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg
     6961 gggactacaa ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca
     7021 aggctaccgc caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca
     7081 ctctggccaa tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg
     7141 agatcgtgtg ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac
     7201 aagtgaatat cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc
     7261 tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt
     7321 acggcgggtt cgacagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga
     7381 agggcaagtc caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc
     7441 gctccagctt cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga
     7501 agaaggacct gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga
     7561 agcggatgct ggcttccgct ggcgagctgc agaaggggaa cgagctggct ctgccgtcca
     7621 agtatgtgaa cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg
     7681 acaacgagca gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg
     7741 agcagatttc cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc
     7801 tctccgcgta caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc
     7861 atctcttcac cctgacgaac ctcggcgccc ctgctgcttt caagtacttc gacacaacta
     7921 tcgatcgcaa gaggtacaca agcactaagg aggtcctgga cgcgaccctc atccaccagt
     7981 cgattaccgg cctctacgag acgcgcatcg acctgtctca gctcgggggc gacaagcggc
     8041 cagcggcgac gaagaaggcg gggcaggcga agaagaagaa gtgagctcag agctttcgtt
     8101 cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca
     8161 ccagaatcct actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt
     8221 tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg
     8281 aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat
     8341 tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac
     8401 attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag
     8461 gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca
     8521 gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt
     8581 tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc attgctttat
     8641 aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta
     8701 tgacttgcca attgattgac aacgaattcg taatcatgtc atagctgttt cctgtgtgaa
     8761 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8821 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8881 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8941 gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc
     9001 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     9061 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     9121 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     9181 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     9241 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg
     9301 tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa
     9361 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
     9421 cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc
     9481 atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag
     9541 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     9601 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     9661 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa
     9721 atcaccagtc tctctctaca aatctatctc tctcgagctt tcgcagatcc ggggggcaat
     9781 gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     9841 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     9901 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     9961 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
    10021 acattgggga gtttagcgag agcctgacct attgcatctc ccgccgttca cagggtgtca
    10081 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct acaaccggtc gcggaggcta
    10141 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
    10201 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
    10261 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
    10321 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
    10381 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
    10441 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
    10501 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
    10561 cgccacgact ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
    10621 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
    10681 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
    10741 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
    10801 caaagaaata gagtagatgc cgaccgggat ctgtcgatcg acaagctcga gtttctccat
    10861 aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg
    10921 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
    10981 aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc
    11041 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
    11101 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
    11161 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
    11221 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
    11281 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
    11341 tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg
    11401 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag
    11461 aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt
    11521 aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat
    11581 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
    11641 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
    11701 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
    11761 agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca
    11821 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
    11881 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
    11941 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
    12001 atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg
    12061 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
    12121 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
    12181 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg
    12241 aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
    12301 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagaatgca
    12361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    12421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    12481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    12541 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    12601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    12661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    12721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    12781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    12841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    12901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    12961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    13021 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    13081 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    13141 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    13201 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
    13261 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
    13321 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
    13381 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    13441 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
    13501 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
    13561 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
    13621 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
    13681 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
    13741 tttaggcgga aaaatcgcct tttttctatt ttatatcagt cacttacatg tgtgaccggt
    13801 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
    13861 ggttcccaat gtacgtgcta tccacaggaa acagaccttt tcgacctttt tcccctgcta
    13921 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
    13981 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
    14041 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
    14101 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
    14161 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
    14221 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
    14281 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
    14341 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
    14401 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
    14461 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
    14521 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
    14581 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
    14641 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
    14701 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
    14761 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
    14821 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    14881 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    14941 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    15001 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
    15061 gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
    15121 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
    15181 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
    15241 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
    15301 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
    15361 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
    15421 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
    15481 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
    15541 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
    15601 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
    15661 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
    15721 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
    15781 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
    15841 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
    15901 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
    15961 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
    16021 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
    16081 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
    16141 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
    16201 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
    16261 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
    16321 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
    16381 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
    16441 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
    16501 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
    16561 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
    16621 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
    16681 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
    16741 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
    16801 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
    16861 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
    16921 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
    16981 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
    17041 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    17101 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    17161 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    17221 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag
    17281 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc
    17341 ttttaaatat ccgattattc taa
//
