LOCUS       Exported               19243 bp ds-DNA     circular SYN 01-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19243)
  AUTHORS   LQP
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 2, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19243
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        271..705
                     /label=35Sen
     promoter        706..1168
                     /label=CmYLCV
     primer_bind     1092..1111
                     /label=CmYLCV-EndF
     promoter        1177..1264
                     /label=U6 (88-bp)
     tRNA            1272..1340
                     /label=tOsGly (2-bp deletion, CA)
     misc_feature    1368..1373
                     /label=B
     misc_feature    1374..1410
                     /label=tevopreQ1 (1-bp deletion, C)
     misc_feature    1411..1478
                     /label=HDV
     terminator      1487..1757
                     /label=HSP18.2t
     primer_bind     complement(1558..1583)
                     /label=HSP-R
     promoter        1780..3772
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             3783..3809
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3816..3869
                     /codon_start=1
                     /label=bpSV40
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             3870..7970
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=nCas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    7977..8024
                     /label=XTEN
     misc_feature    8025..8192
                     /label=NC
     CDS             8193..8213
                     /codon_start=1
                     /label=NLS
                     /translation="PKKKRKV"
     CDS             8220..8315
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             8322..9863
                     /codon_start=1
                     /label=MLV-delRNAseH
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     ADDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPL"
     misc_feature    8391..8693
                     /label=24-124 aa Finger
     misc_feature    8694..8786
                     /label=125-155aa PLAM
     misc_feature    8787..8906
                     /label=156-195aa Finger
     misc_feature    8907..9146
                     /label=196-275 aa PLAM
     misc_feature    9147..9335
                     /label=276-338aa Thumb
     misc_feature    9336..9724
                     /label=339-468aa connection
     CDS             9876..9929
                     /codon_start=1
                     /label=bpNLS
                     /translation="KRTADSQHSTPPKTKRKV"
     CDS             9939..9959
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      9969..10603
                     /label=E9-Ter
     primer_bind     complement(10618..10634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10642..10658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10666..10696)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10711..10732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10923..11599
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             11666..12691
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12732..12906
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12984..13008
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13433..14227
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14314..14902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      15572..15766
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15832..16905)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17334..17963)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgaattc atggagtcaa agattcaaat agaggaccta
      301 acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac
      361 aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
      421 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
      481 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
      541 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
      601 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
      661 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgattggca gacatactgt
      721 cccacaaatg aagatggaat ctgtaaaaga aaacgcgtga aataatgcgt ctgacaaagg
      781 ttaggtcggc tgcctttaat caataccaaa gtggtcccta ccacgatgga aaaactgtgc
      841 agtcggtttg gctttttctg acgaacaaat aagattcgtg gccgacaggt gggggtccac
      901 catgtgaagg catcttcaga ctccaataat ggagcaatga cgtaagggct tacgaaataa
      961 gtaagggtag tttgggaaat gtccactcac ccgtcagtct ataaatactt agcccctccc
     1021 tcattgttaa gggagcaaaa tctcagagag atagtcctag agagagaaag agagcaagta
     1081 gcctagaagt agtcaaggcg gcgaagtatt caggcacgtg gccaggaaga agaaaagcca
     1141 agacgacgaa aacaggtaag agctaagcat ctagataagt tgaaaacaat cttcaaaagt
     1201 cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag tcgaagtagt
     1261 gattgaacaa agcaccagtg gtctagtggt agaatagtac cctgccacgg tacagacccg
     1321 ggttcgattc ccggctggtg tgagaccttc ggggaatatg agcgtcaggt ctccgcggtt
     1381 ctatctagtt acgcgttaaa ccaactagaa ggccggcatg gtcccagcct cctcgctggc
     1441 gccggctggg caacatgctt cggcatggcg aatgggactt ttttttgata tctccggggc
     1501 taattgaata tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag ctggtgtgct
     1561 taagtttgtg tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta
     1621 aatgaatgta agatctcatt ataatgaata aacaaatgtt tctataatcc attgtgaatg
     1681 ttttgttgga tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga
     1741 atatgattaa agataagaag cttggcgtaa tctgcatgcc tgcagtgcag cgtgacccgg
     1801 tcgtgcccct ctctagagat aatgagcatt gcatgtctaa gttataaaaa attaccacat
     1861 attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat atatttaaac
     1921 tttactctac gaataatata atctatagta ctacaataat atcagtgttt tagagaatca
     1981 tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca acaggactct
     2041 acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta
     2101 tataatactt catccatttt attagtacat ccatttaggg tttagggtta atggttttta
     2161 tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat taagaaaact
     2221 aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat aaaataaagt
     2281 gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa catttttctt
     2341 gtttcgagta gataatgcca gcctgttaaa cgccgtcgac gagtctaacg gacaccaacc
     2401 agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat ctctgtcgct
     2461 gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc tgtcggcatc
     2521 cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc tcctcctcct
     2581 ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct ttcccttcct
     2641 cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct cgtgttgttc
     2701 ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac ctccgcttca
     2761 aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc ggcgttccgg
     2821 tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt
     2881 gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg
     2941 attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca
     3001 gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc
     3061 ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg
     3121 tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattctgt
     3181 ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca
     3241 tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat
     3301 gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg
     3361 tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct
     3421 ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt
     3481 taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat
     3541 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct
     3601 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca
     3661 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg
     3721 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc agccctagga
     3781 tgcctgccgc caagagagtc aagctcgatg gcggcaagag gaccgccgat ggcagcgagt
     3841 tcgagtctcc taagaagaag aggaaggtcg acaagaagta ctcgatcggc ctcgatattg
     3901 ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc tcaaagaagt
     3961 tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc
     4021 tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt
     4081 acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac gagatggcga
     4141 aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag gaggataaga
     4201 agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt
     4261 accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag gcggacctcc
     4321 gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc ctgatcgagg
     4381 gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc gtgcagacgt
     4441 acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc
     4501 tgtccgctag gctctcgaag tctcggaagc tcgagaacct gatcgcccag ctgccgggcg
     4561 agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc acgcccaact
     4621 tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag gacacatacg
     4681 acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac ctgttcctcg
     4741 ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg aacaccgaga
     4801 ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac catcaggatc
     4861 tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag gagatcttct
     4921 tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct caggaggagt
     4981 tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag ctgctggtga
     5041 agctcaagcg cgaggacctc ctgaggaagc agcggacatt cgataacggc agcatcccac
     5101 accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac ttctaccctt
     5161 tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc ccgtactacg
     5221 tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag tcagaggaga
     5281 cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca
     5341 tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc cctaagcact
     5401 cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag tatgtgaccg
     5461 agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc gtggacctcc
     5521 tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac ttcaagaaga
     5581 ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac gcctccctgg
     5641 ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac aacgaggaga
     5701 atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac cgggagatga
     5761 tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg aagcagctca
     5821 agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac ggcattcggg
     5881 acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc gcgaaccgca
     5941 atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc cagaaggctc
     6001 aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcgccag
     6061 ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg aaggtcatgg
     6121 gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag accacgcaga
     6181 agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt aaggagctgg
     6241 ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat gagaagctct
     6301 acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg gatattaaca
     6361 ggctcagcga ttacgacgtc gatgccatcg ttccacagtc attcctgaag gatgactcca
     6421 ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat aatgttcctt
     6481 cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat gccaagctga
     6541 tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc tctgagctgg
     6601 acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact aagcacgttg
     6661 cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag ctgatccgcg
     6721 aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag gatttccagt
     6781 tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac ctgaacgcgg
     6841 tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc gtgtacgggg
     6901 actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag attggcaagg
     6961 ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca gagatcactc
     7021 tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag acgggggaga
     7081 tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc atgccacaag
     7141 tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag tcaattctgc
     7201 ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg aagaagtacg
     7261 gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag gttgagaagg
     7321 gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt atggagcgct
     7381 ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag gaggtgaaga
     7441 aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac ggcaggaagc
     7501 ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg ccgtccaagt
     7561 atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc cccgaggaca
     7621 acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcattgagc
     7681 agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat aaggtcctct
     7741 ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat atcattcatc
     7801 tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac acaactatcg
     7861 atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc caccagtcga
     7921 ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac gaattctccg
     7981 ggagcgagac gccaggcacc tccgagtcgg ccaccccaga atctgccaca gtggtgtccg
     8041 gccaaaagca ggaccgccag ggcggagaac gcagaaggtc ccagctcgat agggatcagt
     8101 gtgcctactg caaggagaag ggccactggg ccaaagactg cccgaaaaag ccgcgcggcc
     8161 cacgcggccc aaggccacaa acatccctcc ttccaaagaa gaagcggaag gtggagctca
     8221 gcggaggatc ttccggagga tctagcggct ccgagacacc aggaacatcc gaaagcgcta
     8281 caccagaatc tagcggaggc tcttccggag gatctaggcc taccctcaac atcgaggatg
     8341 agtatcgcct ccacgaaacc tccaaagaac cggacgtgtc cctcggcagc acatggctca
     8401 gcgacttccc acaagcgtgg gccgaaaccg gcggcatggg cctcgccgtc cgccaagccc
     8461 cactcattat cccgctgaag gcgacctcca caccggtgtc catcaagcag tacccgatga
     8521 gccaagaggc gaggctcggg attaagccgc acattcagcg cctcctcgat caaggcattc
     8581 tcgtgccgtg ccaatccccg tggaatacac cactcctccc ggtcaaaaag ccgggcacca
     8641 acgactatcg cccggtccaa gatctccgcg aggtcaacaa gcgcgtggaa gacatccacc
     8701 cgaccgtccc gaacccgtat aatctgctct ccgggctccc accatcccac cagtggtata
     8761 cagtgctgga cctcaaagac gccttcttct gtctccgcct ccacccaaca agccagccgc
     8821 tcttcgcctt cgagtggcgc gacccggaga tgggcatctc cggccaactg acatggacac
     8881 gcctcccgca aggcttcaag aacagcccga cactcttcaa cgaggcgctc catagggacc
     8941 tcgcggattt tcgcatccag catccggacc tcatcctcct ccagtatgcg gatgatctcc
     9001 tcctcgccgc gacctccgag ctggattgtc aacaaggcac acgcgcgctc ctccaaacac
     9061 tcgggaacct cggctatcgc gcgtccgcga aaaaggccca aatctgccag aagcaagtga
     9121 agtacctcgg gtatctgctc aaggaaggcc aacgctggct caccgaagcg cgcaaagaaa
     9181 cagtgatggg gcaaccgaca ccgaaaacac cacgccagct gcgcgagttt ctcggcaaag
     9241 ccggcttctg tcgcctcttc atcccgggct ttgccgagat ggccgcgcca ctctacccac
     9301 tcaccaagcc gggcacactg tttaactggg ggccggatca gcagaaagcc taccaagaga
     9361 tcaaacaagc gctcctcacc gccccagcgc tcgggctccc agatctcaca aagccgttcg
     9421 agctgttcgt cgatgagaag caaggctacg cgaagggcgt gctcacacag aagctcggcc
     9481 cgtggaggag gccagtggcc tatctctcca aaaaactcga tccagtggcc gccggctggc
     9541 caccgtgtct gcgcatggtc gccgcgattg ccgtgctcac aaaggatgcc ggcaaactca
     9601 caatgggcca gccgctggtg atcctcgcgc cacatgccgt ggaagccctc gtcaaacagc
     9661 cgccggatag gtggctctcc aatgcgcgca tgacccatta ccaagcgctc ctcctcgaca
     9721 ccgatcgcgt ccagttcggc ccagtggtcg ccctcaatcc ggcgacactg ctgccactcc
     9781 cagaggaggg cctccaacac aactgtctgg atattctcgc ggaagcgcat ggcacaaggc
     9841 cagacctcac agatcaaccg ctcagcggcg gcagcaagag gaccgccgac tcccagcact
     9901 cgaccccgcc gaagaccaag aggaaggtgg agttcgagcc gaagaagaag aggaaggtct
     9961 gagagctcag agctttcgtt cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca
    10021 tcagtttcat tgcgcacaca ccagaatcct actgagtttg agtattatgg cattgggaaa
    10081 actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta ttcggttttc
    10141 gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata tggtcctttt
    10201 gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa
    10261 attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta
    10321 atgaccgaag ttaatatgag gagtaaaaca cttgtagttg taccattatg cttattcact
    10381 aggcaacaaa tatattttca gacctagaaa agctgcaaat gttactgaat acaagtatgt
    10441 cctcttgtgt tttagacatt tatgaacttt cctttatgta attttccaga atccttgtca
    10501 gattctaatc attgctttat aattatagtt atactcatgg atttgtagtt gagtatgaaa
    10561 atatttttta atgcatttta tgacttgcca attgattgac aacgaattcg taatcatgtc
    10621 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    10681 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    10741 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    10801 ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc agcttgccaa catggtggag
    10861 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct
    10921 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
    10981 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat
    11041 tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga
    11101 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
    11161 gtggattgat gtgaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
    11221 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa
    11281 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga
    11341 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
    11401 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
    11461 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
    11521 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
    11581 tttggagagg acacgctgaa atcaccagtc tctctctaca aatctatctc tctcgagctt
    11641 tcgcagatcc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga
    11701 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
    11761 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
    11821 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
    11881 cccgattccg gaagtgcttg acattgggga gtttagcgag agcctgacct attgcatctc
    11941 ccgccgttca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
    12001 acaaccggtc gcggaggcta tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
    12061 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
    12121 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
    12181 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
    12241 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
    12301 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
    12361 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
    12421 gcatccggag cttgcaggat cgccacgact ccgggcgtat atgctccgca ttggtcttga
    12481 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
    12541 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
    12601 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
    12661 ccccagcact cgtccgaggg caaagaaata gagtagatgc cgaccgggat ctgtcgatcg
    12721 acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt
    12781 cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
    12841 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
    12901 cagatccccc gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta
    12961 ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa
    13021 cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc
    13081 cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg
    13141 ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg
    13201 tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa
    13261 tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt
    13321 tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat
    13381 tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa
    13441 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
    13501 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
    13561 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
    13621 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
    13681 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
    13741 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
    13801 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt
    13861 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac
    13921 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
    13981 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
    14041 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
    14101 ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
    14161 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
    14221 gttttagtac ctagaatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    14281 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    14341 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    14401 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    14461 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    14521 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    14581 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    14641 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    14701 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    14761 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    14821 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    14881 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    14941 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    15001 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    15061 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
    15121 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    15181 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
    15241 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
    15301 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
    15361 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
    15421 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
    15481 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
    15541 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
    15601 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
    15661 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
    15721 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa acagaccttt
    15781 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
    15841 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
    15901 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
    15961 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
    16021 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
    16081 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
    16141 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
    16201 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
    16261 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
    16321 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
    16381 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
    16441 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
    16501 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
    16561 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
    16621 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
    16681 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
    16741 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
    16801 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
    16861 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
    16921 ttgggggttc cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa
    16981 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
    17041 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
    17101 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
    17161 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
    17221 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
    17281 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
    17341 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
    17401 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
    17461 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
    17521 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
    17581 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
    17641 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
    17701 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
    17761 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
    17821 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    17881 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    17941 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    18001 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    18061 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    18121 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    18181 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    18241 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    18301 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    18361 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    18421 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    18481 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    18541 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    18601 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    18661 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    18721 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    18781 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    18841 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    18901 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    18961 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    19021 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    19081 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    19141 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
    19201 ggataaacct tttcacgccc ttttaaatat ccgattattc taa
//
