LOCUS       #147_pHSV_VAMP7_        6901 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Alonso
  TITLE     Direct Submission
  JOURNAL   Exported Nov 24, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     Sequence Label: pHSV_ELGA
FEATURES             Location/Qualifiers
     source          1..6901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    186..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1470..1494
                     /label=Primer 17#F
     regulatory      1499..1508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1504..1526
                     /label=487#EcoRI_Vamp7_F
     CDS             1505..3601
                     /codon_start=1
                     /label=ELGA
                     /translation="MAILFAVVARGTTILAKHAWCGGNFLEVTEQILAKIPSENNKLTY
                     SHGNYLFHYICQDRIVYLCITDDDFERSRAFNFLNEIKKRFQTTYGSRAQTALPYAMNS
                     EFSSVLAAQLKHHSENKGLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTEN
                     LVDSSVTFKTTSRNLARAMCMKNLKLTIIIIIVSIVFIYIIVSPLCGGFTWPSCVKKKL
                     SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTL
                     VTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTL
                     VNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKANFKIRHNIEDGSVQL
                     ADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELY
                     KSGGSGSGGQSGGSGSGGQSGGSGSGGQSGGSGSGGQSGGSGSGGQSGLRSVKLTSDFD
                     NPRWIGRHKHMFNFLDVNHNGKISLDEMVYKASDIVINNLGATPEQAKRHKDAVEAFFG
                     GAGMKYGVETDWPAYIEGWKKLATDELEKYAKNEPTLIRIWGDALFDIVDKAQNGAITL
                     DEWKAYTKAAGIIQSSEDCEETFRVCDIDESGQLDVDEMTRQHLGFWYTMDPACEKLYG
                     GAVP"
     CDS             1505..2164
                     /codon_start=1
                     /label=VAMP7
                     /note="VAMP"
                     /translation="MAILFAVVARGTTILAKHAWCGGNFLEVTEQILAKIPSENNKLTY
                     SHGNYLFHYICQDRIVYLCITDDDFERSRAFNFLNEIKKRFQTTYGSRAQTALPYAMNS
                     EFSSVLAAQLKHHSENKGLDKVMETQAQVDELKGIMVRNIDLVAQRGERLELLIDKTEN
                     LVDSSVTFKTTSRNLARAMCMKNLKLTIIIIIVSIVFIYIIVSPLCGGFTWPSCVKK"
     misc_feature    2170..3026
                     /label=EGFP
                     /note="EGFP"
     misc_feature    3038..3602
                     /label=mutAEQ
     misc_feature    3386..3388
                     /label=D119A
                     /note="Mutation that reduces affinity for Ca"
     polyA_signal    3922..4055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5177..5281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5282..5350,5351..6142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6313..6901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gccattatgc
      481 acgaccccgc cccgacgccg gcacgccggg ggcccgtggc cgcggcccgt tggtcgaacc
      541 cccggccccg cccatccgcg ccatctgcca tgggcggggc gcgagggcgg gtgggtccgc
      601 gccccgcccc gcatggcatc tcattaccgc ccgatccggc ggtttccgct tccgttccgc
      661 atgctaacga ggaacgggca gggggcgggg cccgggcccc gacttcccgg ttcggcggta
      721 atgagatacg agccccgcgc gcccgttggc cgtccccggg ccccccggtc ccgcccgccg
      781 gacgccggga ccaacgggac ggcgggcggc ccaagggccg cccgccttgc cgccccccca
      841 ttggccggcg ggcgggaccg ccccaagggg gcggggccgc cgggtaaaag aagtgagaac
      901 gcgaagcgtt cgcacttcgt cccaatatat atatattatt agggcgaagt gcgagcactg
      961 gcgccgtgcc cgactccgcg ccggccccgg gggcgggccc gggcggcggg gggcgggtct
     1021 ctccggcgca cataaaggcc cggcgcgacc gacgcccgca gacggcgccg gccacgaacg
     1081 acgggagcgg ctgcggagca cgcggaccgg gagcgggagt cgcagagggc cgtcggagcg
     1141 gacggcgtcg gcatcgcgac gccccggctc gggatcggga tcgcatcgga aagggacacg
     1201 cggacgcggg ggggaaagac ccgcccaccc cacccacgaa acacagggga cgcaccccgg
     1261 gggcctccga cgacagaaac ccaccggtcc gccttttttg cacgggtaag caccttgggt
     1321 gggcggagga gggggggacg cgggggtgga ggagggggga cgcgggggcg gaggaggggg
     1381 gacgcggggg cggaggaggg gggacgcggg ggcggaggag gggggacgcg ggggcggagg
     1441 agggggctca cccgcgttcg tgccttcccg caggaggaac gtcctcgtcg ataagcttgc
     1501 caccatggcg attctttttg ctgttgttgc cagggggacc actatccttg ccaaacatgc
     1561 ttggtgtgga ggaaacttcc tggaggtgac agagcagatt ctggctaaga taccttctga
     1621 aaataacaaa ctaacgtact cacatggcaa ttatttgttt cattacatct gccaagacag
     1681 gattgtatat ctttgtatca ctgatgatga ttttgaacgt tcccgagcct ttaattttct
     1741 gaatgagata aagaagaggt tccagactac ttacggttca agagcacaga cagcacttcc
     1801 atatgccatg aatagcgagt tctcaagtgt cttagctgca cagctgaagc atcactctga
     1861 gaataagggc ctagacaaag tgatggagac tcaagcccaa gtggatgaac tgaaaggaat
     1921 catggtcaga aacatagatc tggtagctca gcgaggagaa agattggaat tattgattga
     1981 caaaacagaa aatcttgtgg attcttctgt caccttcaaa actaccagca gaaatcttgc
     2041 tcgagccatg tgtatgaaga acctcaagct cactattatc atcatcatcg tatcaattgt
     2101 gttcatctat atcattgttt cacctctctg tggtggattt acatggccaa gctgtgtgaa
     2161 gaaaaagctt agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2221 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2281 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2341 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2401 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2461 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2521 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2581 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2641 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2701 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2761 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2821 ggtcctgctg gagttcgtga ccgccgccgg gatcactcac ggcatggacg agctgtacaa
     2881 gtccggcggg agcggatccg gcggccagtc cggcgggagc ggatccggcg gccagtccgg
     2941 cgggagcgga tccggcggcc agtccggcgg gagcggatcc ggcggccagt ccggcgggag
     3001 cggatccggc ggccagtccg gactcagatc tgtcaaactt acatcagact tcgacaaccc
     3061 aagatggatt ggacgacaca agcatatgtt caatttcctt gatgtcaacc acaatggaaa
     3121 aatctctctt gacgagatgg tctacaaggc atctgatatt gtcatcaata accttggagc
     3181 aacacctgag caagccaaac gacacaaaga tgctgtggaa gccttcttcg gaggagctgg
     3241 aatgaaatat ggtgtggaaa ctgattggcc tgcatatatt gaaggatgga aaaaattggc
     3301 tactgatgaa ttggagaaat acgccaaaaa cgaaccaacc ctcatccgca tctggggtga
     3361 tgctttgttt gatatcgtcg acaaagccca aaatggagcc attacactgg atgaatggaa
     3421 agcatacacc aaagctgctg gtatcatcca atcatcagaa gattgcgagg aaacattcag
     3481 agtgtgcgat attgatgaaa gtggacaact cgatgttgat gagatgacaa gacaacattt
     3541 aggattttgg tacaccatgg atcctgcttg cgaaaagctc tacggtggag ctgtccccta
     3601 agaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata
     3661 ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt
     3721 aaaaaacaca aacttttgga tgttcggttt attctttttc ttttactttt ttatcatggg
     3781 agcctacttc ccgtttttcc cgatttggct acatgacatc aaccatatca gcaaaagtga
     3841 tacgggtatt atttttgccg ctatttctct gttctcgcta ttattccaac cgctgtttgg
     3901 tctgctttct gacaaactcg gaacttgttt attgcagctt ataatggtta caaataaagc
     3961 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggttgt
     4021 ccaaactcat caatgtatct tatcatgtct ggatctctga cctgagattg gcggcactga
     4081 ggtagagatg cccgaacccc cccgagggag cgcgggacgc gccggggagg gctggggccg
     4141 gggagggctg gggccgggga gggctggggc cggggagggc tggggccggg gagggctggg
     4201 gccggggagg gctggggccg gggagggctg gggctgggga gggctggggc tggggagggg
     4261 gcggtggtgt gtagcaggag cggtgtgttg cgccggggta cgtctggagg agcgggaggt
     4321 gcgcggtgac gtgtggatga ggaacaggag ttgttgcgcg gtgagttgtc gctgtgagtt
     4381 gtgttgttgg gcaggtgtgg tggatgacgt gacgtgtgac gtgcggagtg cgccgtgctc
     4441 tgttggtttc acctgtggca gcccgggccc cccgcgggcg cgcgcgcgcg caaaaaaggc
     4501 gggcggcggt ccgggcggcg tgcgcgcgcg cggcgggcgt ggggggcggg gccgcgggag
     4561 cggggggagg agcgggggga ggagcggggg gaggagcggg gggaggagcg gggggaggag
     4621 cggggggagg agcgggggga ggagcggggg gaggagcggg gggaggagcg gggggaggag
     4681 cggggggagg agcgggggga ggagcggggg gaggagcggg gggaggagcg gggggaggag
     4741 cggggggagg agcgggggga ggagcggggg gaggagcggc cagacgccga aaacgggccc
     4801 cccccaaaac acaccccccg ggggtcgcgc gcggcccttt aaagcggtgg cggcgggcag
     4861 cccgggcccc ccgcggccga gactagcgag ttagacaggc aagcactact cgcctctgca
     4921 cgcacatgct tgcctgtcaa actctaccac cccggcacgc tctctgtctc catggcccgc
     4981 cgccgccgcc atcgcggccc ccgccgcccc cggccgcccg ggcccacggg cgccgtccca
     5041 accgcacagt cccaggtaac gagctcgaat taattcttga agacgaaagg gcctcgtgat
     5101 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5161 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5221 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5281 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5341 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5401 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5461 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5521 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5581 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5641 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5701 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5761 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5821 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5881 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5941 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6001 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6061 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6121 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6181 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6241 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6301 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6361 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6421 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     6481 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6541 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6601 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6661 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6721 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6781 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6841 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6901 a
//
