LOCUS       pUC_M1      3079 bp    DNA     circular UNA 14-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence (unknown)
  ORGANISM  natural DNA sequence
            unspecified
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2025 from SnapGene Viewer 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     rep_origin      complement(join(2863..3079,1..372))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
     source          1..3079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(543..1403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="This feature has 2 segments: 1:  543 .. 1334 /
                     #ccffcc 2: 1335 .. 1403 / #ccffcc / signal sequence
                     Cleavage site after base 1334"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(1404..1508)
                     /gene="bla"
                     /label="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     1937..1960
                     /note="Primer: GGCGATTAAGTTGGGTAACGCCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on pUC_M1: 1"
                     /note="Primer binding region: 1937..1960"
                     /label="pUC-primer+3 (mismatches: 0)"
     primer_bind     1955..1978
                     /note="Primer: CGCCAGGGTTTTCCCAGTCACGAC"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on pUC_M1: 0"
                     /note="Primer binding region: 1955..1978"
                     /label="pUC-Primer 1224 (mismatches: 0)"
     primer_bind     1978..1997
                     /note="Primer: CGTTGTAAAACGACGGCCAG"
                     /note="Number of mismatches: 0"
                     /note="Number of other matches on pUC_M1: 0"
                     /note="Primer binding region: 1978..1997"
                     /label="pUC-primer+2 (mismatches: 0)"
     primer_bind     1982..1998
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    2005..2021
                     /label="T7 promoter"
                     /note="color: #a6acb3"
     CDS             2022..2398
                     /label="M1RNA"
                     /note="color: #a6acb3"
     primer_bind     complement(2461..2477)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(2473..2496)
                     /note="Primer: AGCGGATAACAATTTCACACAGGA"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on pUC_M1: 0"
                     /note="Primer binding region: complement(2473..2496)"
                     /label="pUC-Primer 1233 (mismatches: 0)"
     protein_bind    2485..2501
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     complement(2504..2523)
                     /note="Primer: CCGGCTCGTATGTTGTGTGG"
                     /note="Number of mismatches: 0"
                     /note="Number of other hits on pUC_M1: 0"
                     /note="Primer binding region: complement(2504..2523)"
                     /label="pUC-primer-1 (mismatches: 0)"
     protein_bind    2554..2575
                     /label="CAP binding site"
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presence of cAMP."
                     /note="color: #31849b"
ORIGIN
        1 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
       61 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
      121 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
      181 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
      241 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
      301 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
      361 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
      421 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
      481 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
      541 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
      601 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
      661 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
      721 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
      781 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
      841 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
      901 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
      961 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     1021 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     1081 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     1141 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     1201 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     1261 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     1321 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     1381 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     1441 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     1501 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG
     1561 ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT
     1621 GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG
     1681 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC
     1741 TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA
     1801 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG
     1861 CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA
     1921 GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT
     1981 TGTAAAACGA CGGCCAGTGA ATTCTAATAC GACTCACTAT AGAAGCTGAC CAGACAGTCG
     2041 CCGCTTCGTC GTCGTCCTCT TCGGGGGAGA CGGGCGGAGG GGAGGAAAGT CCGGGCTCCA
     2101 TAGGGCAGGG TGCCAGGTAA CGCCTGGGGG GGAAACCCAC GACCAGTGCA ACAGAGAGCA
     2161 AACCGCCGAT GGCCCGCGCA AGCGGGATCA GGTAAGGGTG AAAGGGTGCG GTAAGAGCGC
     2221 ACCGCGCGGC TGGTAACAGT CCGTGGCACG GTAAACTCCA CCCGGAGCAA GGCCAAATAG
     2281 GGGTTCATAA GGTACGGCCC GTACTGAACC CGGGTAGGCT GCTTGAGCCA GTGAGCGATT
     2341 GCTGGCCTAG ATGAATGACT GTCCACGACA GAACCCGGCT TATCGGTCAG TTTCACCTGA
     2401 TTTACGTCAT CCGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGG CGTAATCATG
     2461 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC
     2521 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC
     2581 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT
     2641 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
     2701 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
     2761 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
     2821 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
     2881 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     2941 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     3001 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     3061 CTCACGCTGT AGGTATCTC
//
