LOCUS       pLVX-Puro       10062 bp DNA     circular SYN 03-SEP-2025
DEFINITION  Lentiviral expression vector with the constitutive CMV promoter and 
            a puromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Sequence Label: pLVX-Puro
FEATURES             Location/Qualifiers
     source          1..10062
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2820
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          4117..4834
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2809..2844
                     /label=Primer 1
                     /note="color: black; sequence: 
                     TCAGATCTCGAGGCCACCATGTTCAGTCTCATGGCA; added: 2025-09-03"
     misc_feature    2815..4839
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     misc_feature    2820
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein
                     plasmids"
                     /note="color: #99ccff"
     misc_feature    join(2827..3055,3056..4110)
                     /label=Arl13b-Res
                     /note="This forward directional feature has 2 segments:
                        1: 2827 .. 3055 / #ff6600
                        2: 3056 .. 4110 / #ff6600"
     primer_bind     complement(3029..3067)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     TTCCCCGAATTCTTATCTGACCTCCCAAGTCAAAGATGG; added: 2025-09-03"
     variation       3049..3051
                     /label=G75Q
                     /note="color: #a6acb3"
     CDS             join(4117..4119,4120..4122,4123..4834)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 4117 .. 4119 / #05fd14
                        2: 4120 .. 4122 / #05fd14 / 1a
                        3: 4123 .. 4834 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        4850..5349
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5370..5969
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5983..6571
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     LTR             6778..7411
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     rep_origin      complement(7942..8530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(8701..9492,9493..9561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8701 .. 9492 / #ccffcc
                        2: 9493 .. 9561 / #ccffcc / signal sequence
                     Cleavage site after base 9492"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9562..9666)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgt tcagtctcat ggcaagctgt tgtggatggt ttaaacgctg gcgggagcct
     2881 gtcagaaagg tgactctttt gatggtggga cttgataatg ctggtaaaac cgcaacagca
     2941 aagggaatcc aaggagaata ccctgaagat gtagctccta ctgttggatt ttcaaaaatt
     3001 aaccttagac aaggaaagtt tgaagtcacc atctttgact tgggaggtca gataagaatt
     3061 cggggaatct ggaagaatta ctatgctgaa tcctatgggg taatatttgt tgtggattcc
     3121 agtgatgaag agagaatgga agagacaaaa gaggctatgt cagaaatgct aagacatcct
     3181 aggatatcgg gaaagcctat attggtgttg gcaaataaac aagataaaga aggagcttta
     3241 ggagaagctg atgtcattga atgtctatct ctggaaaaat tggtcaatga gcacaagtgc
     3301 ctgtgtcaga tagaaccatg ctccgccatt tccggctatg gaaagaaaat tgacaagtcc
     3361 attaaaaaag gcctttattg gctgctacat gttattgcaa gagactttga tgccttaaat
     3421 gaacgcatcc aaaaagagac aacagagcag cgtgctcttg aggaacaaga gaaacaagaa
     3481 agagctgaac gagtgcgaaa attacgagaa gaaagaaaac aaaatgaaca ggagcaggct
     3541 gaactcgatg gaaccagtgg tctggctgag ttggacccag aaccaacgaa tcctttccag
     3601 ccaatagcat ctgtaatcat tgagaatgaa ggaaaacttg aaagagagaa aaaaaaccaa
     3661 aaaatggaga aagacagtga tggctgccac ctgaaacata aaatggagca tgagcaaata
     3721 gagacacaag gccaggttaa tcacaatggc caaaaaaata atgaatttgg actagtagaa
     3781 aattataagg aggcattaac acagcagtta aagaatgaag atgagacaga ccggccatca
     3841 ttggaatcag ctaatggtaa aaagaaaact aagaaactaa gaatgaaaag gaaccaccgg
     3901 gtagaaccac ttaatataga tgactgtgct cctgagagtc caacgccacc cccaccccct
     3961 cctcctgttg gctggggaac ccctaaagtc actagacttc caaaacttga gcctcttggt
     4021 gaaacacatc ataatgattt ctataggaag ccactgcctc ccctggctgt gccacagcga
     4081 cctaacagtg atgctcatga tgtgatctca ggatccatgg tgagcaaggg cgaggagctg
     4141 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4201 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     4261 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     4321 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4381 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4441 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4501 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4561 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     4621 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4681 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     4741 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4801 gggatcactc tcggcatgga cgagctgtac aagtctagat aattctaccg ggtaggggag
     4861 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     4921 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     4981 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     5041 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     5101 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     5161 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     5221 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     5281 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     5341 ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg
     5401 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5461 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     5521 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5581 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     5641 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     5701 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     5761 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     5821 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     5881 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     5941 ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc tctggattac
     6001 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6061 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6121 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6181 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6241 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6301 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6361 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     6421 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6481 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6541 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
     6601 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6661 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6721 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
     6781 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     6841 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     6901 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     6961 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     7021 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     7081 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     7141 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     7201 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7381 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7441 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
     7501 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7561 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7621 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7681 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7741 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7801 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7861 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8341 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8581 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8641 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8701 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8761 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8821 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8881 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8941 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9001 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9061 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9121 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9181 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9241 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9301 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9361 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9421 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9481 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9541 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9601 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9661 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt
     9721 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9781 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9841 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     9901 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     9961 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta
    10021 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
