LOCUS       pLVX-Puro       10056 bp DNA     circular SYN 03-SEP-2025
DEFINITION  Lentiviral expression vector with the constitutive CMV promoter and 
            a puromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Sequence Label: pLVX-Puro
FEATURES             Location/Qualifiers
     source          1..10056
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2820
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          4111..4828
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2820
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein
                     plasmids"
                     /note="color: #99ccff"
     misc_feature    2821..4104
                     /label=Arl13b-Res
                     /note="color: #ff6600; direction: RIGHT"
     variation       2842..2847
                     /label=C8C9-SS
                     /note="color: #a6acb3"
     CDS             join(4111..4113,4114..4116,4117..4828)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 4111 .. 4113 / #05fd14
                        2: 4114 .. 4116 / #05fd14 / 1a
                        3: 4117 .. 4828 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        4844..5343
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5364..5963
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5977..6565
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     LTR             6772..7405
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     rep_origin      complement(7936..8524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(8695..9486,9487..9555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8695 .. 9486 / #ccffcc
                        2: 9487 .. 9555 / #ccffcc / signal sequence
                     Cleavage site after base 9486"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9556..9660)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 atgttcagtc tcatggcaag cagtagcgga tggtttaaac gctggcggga gcctgtcaga
     2881 aaggtgactc ttttgatggt gggacttgat aatgctggta aaaccgcaac agcaaaggga
     2941 atccaaggag aataccctga agatgtagct cctactgttg gattttcaaa aattaacctt
     3001 agacaaggaa agtttgaagt caccatcttt gacttgggag gtggaataag aattcgggga
     3061 atctggaaga attactatgc tgaatcctat ggggtaatat ttgttgtgga ttccagtgat
     3121 gaagagagaa tggaagagac aaaagaggct atgtcagaaa tgctaagaca tcctaggata
     3181 tcgggaaagc ctatattggt gttggcaaat aaacaagata aagaaggagc tttaggagaa
     3241 gctgatgtca ttgaatgtct atctctggaa aaattggtca atgagcacaa gtgcctgtgt
     3301 cagatagaac catgctccgc catttccggc tatggaaaga aaattgacaa gtccattaaa
     3361 aaaggccttt attggctgct acatgttatt gcaagagact ttgatgcctt aaatgaacgc
     3421 atccaaaaag agacaacaga gcagcgtgct cttgaggaac aagagaaaca agaaagagct
     3481 gaacgagtgc gaaaattacg agaagaaaga aaacaaaatg aacaggagca ggctgaactc
     3541 gatggaacca gtggtctggc tgagttggac ccagaaccaa cgaatccttt ccagccaata
     3601 gcatctgtaa tcattgagaa tgaaggaaaa cttgaaagag agaaaaaaaa ccaaaaaatg
     3661 gagaaagaca gtgatggctg ccacctgaaa cataaaatgg agcatgagca aatagagaca
     3721 caaggccagg ttaatcacaa tggccaaaaa aataatgaat ttggactagt agaaaattat
     3781 aaggaggcat taacacagca gttaaagaat gaagatgaga cagaccggcc atcattggaa
     3841 tcagctaatg gtaaaaagaa aactaagaaa ctaagaatga aaaggaacca ccgggtagaa
     3901 ccacttaata tagatgactg tgctcctgag agtccaacgc cacccccacc ccctcctcct
     3961 gttggctggg gaacccctaa agtcactaga cttccaaaac ttgagcctct tggtgaaaca
     4021 catcataatg atttctatag gaagccactg cctcccctgg ctgtgccaca gcgacctaac
     4081 agtgatgctc atgatgtgat ctcaggatcc atggtgagca agggcgagga gctgttcacc
     4141 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4201 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4261 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     4321 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4381 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4441 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4501 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4561 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4621 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4681 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4741 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4801 actctcggca tggacgagct gtacaagtct agataattct accgggtagg ggaggcgctt
     4861 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     4921 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4981 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     5041 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     5101 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     5161 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     5221 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     5281 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5341 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5401 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5461 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     5521 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5581 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     5641 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     5701 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     5761 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     5821 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     5881 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     5941 tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt
     6001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6361 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6421 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6481 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6541 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     6601 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     6661 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6721 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     6781 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6841 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     6901 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     6961 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7021 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     7081 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     7141 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     7201 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     7261 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7321 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7381 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7441 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc
     7501 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7561 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7621 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7681 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7741 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7801 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7861 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7921 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8341 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8581 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8641 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8701 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8761 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8821 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8881 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8941 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9001 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9061 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9121 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9181 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9241 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9301 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9361 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9421 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9481 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9541 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9601 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9661 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
     9721 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9781 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9841 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     9901 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     9961 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt tttaaagaaa
    10021 ttgtatttgt taaatatgta ctacaaactt agtagt
//
