LOCUS       pLVX-Puro        9775 bp DNA     circular SYN 03-SEP-2025
DEFINITION  Lentiviral expression vector with the constitutive CMV promoter and 
            a puromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9775)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Sequence Label: pLVX-Puro
FEATURES             Location/Qualifiers
     source          1..9775
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2820
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          3830..4547
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2809..2844
                     /label=Primer 1
                     /note="color: black; sequence: 
                     TCAGATCTCGAGGCCACCATGTTCAGTCTCATGGCA; added: 2025-09-03"
     primer_bind     2809..2844
                     /label=Overlap-F
                     /note="color: black; sequence: 
                     TCAGATCTCGAGGCCACCATGTTCAGTCTCATGGCA; added: 2025-09-03"
     primer_bind     2809..2829
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: TCAGATCTCGAGGCCACCATG; 
                     added: 2025-09-03"
     misc_feature    2815..4552
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     misc_feature    2820
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein
                     plasmids"
                     /note="color: #99ccff"
     misc_feature    2827..3561
                     /label=Arl13b-Res
                     /note="color: #ff6600; direction: RIGHT"
     misc_feature    3400..3561
                     /label=CC
                     /note="color: #a6acb3"
     primer_bind     complement(3535..3575)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     GTCTCGCGGCCGCTACCACTGGTTCCATCGAGTTCAGCCTG; added: 
                     2025-09-03"
     primer_bind     complement(3562..3584)
                     /label=Fragment 1.REV
                     /note="color: black; sequence: CGCGAGACTGTCTCGCGGCCGCT; 
                     added: 2025-09-03"
     primer_bind     complement(3563..3585)
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: CCGCGAGACAGTCTCGCGGCCGC; 
                     added: 2025-09-03"
     primer_bind     3567..3589
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: CCGCGAGACAGTCTCGCGGCCGC; 
                     added: 2025-09-03"
     primer_bind     3576..3611
                     /label=Primer 1 (1)
                     /note="color: black; sequence: 
                     AGTCTCGCGGCCGCCTAAGAATGAAAAGGAACCACC; added: 2025-09-03"
     misc_feature    3590..3823
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     misc_feature    3590..3823
                     /label=Arl13b-Res
                     /note="color: #ff6600; direction: RIGHT"
     misc_feature    3611..3622
                     /label=RVEP motif
                     /note="color: #993300"
     misc_feature    3644..3796
                     /label=PRR
                     /note="color: #800000"
     primer_bind     complement(3801..3829)
                     /label=Primer 2 (1)
                     /note="color: black; sequence: 
                     AGTCTCGGATCCTGAGATCACATCATGAGCATCAC; added: 2025-09-03"
     primer_bind     complement(3801..3829)
                     /label=Overlap-R
                     /note="color: black; sequence: 
                     AGTCTCGGATCCTGAGATCACATCATGAGCATCAC; added: 2025-09-03"
     primer_bind     complement(3811..3829)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: AGTCTCGGATCCTGAGATCACATCA; 
                     added: 2025-09-03"
     CDS             join(3830..3832,3833..3835,3836..4547)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3830 .. 3832 / #05fd14
                        2: 3833 .. 3835 / #05fd14 / 1a
                        3: 3836 .. 4547 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        4563..5062
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5083..5682
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5149..5159
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: CCGCGAGACAGTCTCGCGGCCGC; 
                     added: 2025-09-03"
     misc_feature    5696..6284
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     LTR             6491..7124
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     rep_origin      complement(7655..8243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(8414..9205,9206..9274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8414 .. 9205 / #ccffcc
                        2: 9206 .. 9274 / #ccffcc / signal sequence
                     Cleavage site after base 9205"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9275..9379)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gccaccatgt tcagtctcat ggcaagctgt tgtggatggt ttaaacgctg gcgggagcct
     2881 gtcagaaagg tgactctttt gatggtggga cttgataatg ctggtaaaac cgcaacagca
     2941 aagggaatcc aaggagaata ccctgaagat gtagctccta ctgttggatt ttcaaaaatt
     3001 aaccttagac aaggaaagtt tgaagtcacc atctttgact tgggaggtgg aataagaatt
     3061 cggggaatct ggaagaatta ctatgctgaa tcctatgggg taatatttgt tgtggattcc
     3121 agtgatgaag agagaatgga agagacaaaa gaggctatgt cagaaatgct aagacatcct
     3181 aggatatcgg gaaagcctat attggtgttg gcaaataaac aagataaaga aggagcttta
     3241 ggagaagctg atgtcattga atgtctatct ctggaaaaat tggtcaatga gcacaagtgc
     3301 ctgtgtcaga tagaaccatg ctccgccatt tccggctatg gaaagaaaat tgacaagtcc
     3361 attaaaaaag gcctttattg gctgctacat gttattgcaa gagactttga tgccttaaat
     3421 gaacgcatcc aaaaagagac aacagagcag cgtgctcttg aggaacaaga gaaacaagaa
     3481 agagctgaac gagtgcgaaa attacgagaa gaaagaaaac aaaatgaaca ggagcaggct
     3541 gaactcgatg gaaccagtgg tagcggccgc gagacagtct cgcggccgcc taagaatgaa
     3601 aaggaaccac cgggtagaac cacttaatat agatgactgt gctcctgaga gtccaacgcc
     3661 acccccaccc cctcctcctg ttggctgggg aacccctaaa gtcactagac ttccaaaact
     3721 tgagcctctt ggtgaaacac atcataatga tttctatagg aagccactgc ctcccctggc
     3781 tgtgccacag cgacctaaca gtgatgctca tgatgtgatc tcaggatcca tggtgagcaa
     3841 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3901 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3961 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4021 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4081 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4141 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4201 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4261 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4321 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4381 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4441 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4501 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtcta gataattcta
     4561 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     4621 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     4681 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     4741 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     4801 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     4861 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     4921 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     4981 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     5041 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     5101 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5161 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5221 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5281 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5341 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5401 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5461 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5521 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5581 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5641 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca
     5701 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5761 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5821 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5881 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5941 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6001 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6061 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     6121 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6181 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6241 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     6301 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6361 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     6421 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6481 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6541 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     6601 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     6661 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     6721 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     6781 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     6841 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     6901 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     6961 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7021 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7081 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7141 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
     7201 acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7381 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8401 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8461 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8521 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8581 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8641 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8701 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8761 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8821 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8881 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8941 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9001 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9061 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9121 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9181 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9241 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9301 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9361 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     9421 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9541 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     9601 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     9661 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     9721 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
