LOCUS       Exported                9891 bp ds-DNA     circular SYN 03-SEP-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 3, 2025 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          9883..9885
                     /label=source
                     /note="/ApEinfo_hidden="
                     /note="/dnas_title=pHR-BFP-NLS-P2A-NS3pro-WPRE"
                     /note="/locus_tag=source"
                     /note="color: #00df87"
     CDS             1236..2096
                     /locus_tag="bla (AmpR)"
                     /label=bla (AmpR)
                     /note="/dnas_title=bla (AmpR)"
                     /note="/vntifkey=4"
                     /note="color: #ff8040; direction: RIGHT"
     repeat_region   7523..7702
                     /locus_tag="cPPT"
                     /label=cPPT
                     /note="/dnas_title=cPPT"
                     /note="/vntifkey=34"
                     /note="color: #ffc0cb"
     primer_bind     7678..7701
                     /label=cPPT_5'seq
                     /note="color: black; sequence: cagggacagcagagatccagtttg"
     promoter        7735..8226
                     /locus_tag="SFFV"
                     /label=SFFV
                     /note="/dnas_title=SFFV"
                     /note="/vntifkey=29"
                     /note="color: #ffc0cb"
     primer_bind     8151..8174
                     /label=1166_seq
                     /note="color: black; sequence: gcttcccgagctctataaaagagc"
     misc_feature    8266..8268
                     /locus_tag="ATG"
                     /label=ATG
                     /note="color: #ff8080"
     misc_feature    8269..8964
                     /locus_tag="BFP"
                     /label=BFP
                     /note="color: #0000ff"
     misc_feature    8995..9015
                     /locus_tag="NLS"
                     /label=NLS
                     /note="color: #ffff00"
     misc_feature    9016..9081
                     /locus_tag="P2A"
                     /label=P2A
                     /note="color: #0000ff"
     CDS             9088..9306
                     /locus_tag="P"
                     /label=P
                     /note="Length: 726"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
                     /note="/cds_type=ORF"
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    9307..9324
                     /locus_tag="linker(1)"
                     /label=linker
                     /label=linker(1)
                     /note="linker"
                     /note="color: #ffff00"
     misc_feature    9325..9363
                     /locus_tag="ALFA tag(human codon optimised)"
                     /label=ALFA tag(human codon optimised)
                     /note="color: #ff0000"
     misc_feature    9364..9381
                     /locus_tag="linker"
                     /label=linker
                     /note="linker"
                     /note="color: #ffff00"
     CDS             9382..9882
                     /locus_tag="P(1)"
                     /label=P
                     /label=P(1)
                     /note="Length: 726"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
                     /note="/cds_type=ORF"
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    9883..9885
                     /locus_tag="STOP"
                     /label=STOP
                     /note="color: #ff8080"
ORIGIN
        1 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
       61 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
      121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      301 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
      361 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
      421 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
      481 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
      541 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
      601 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      661 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      721 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
      781 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
      841 gagcgtcgaa tggtgcactc tcagtacaat ctagctctga tgccgcatag ttaagccagc
      901 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      961 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     1021 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1741 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2941 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3001 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3061 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     3121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3181 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3241 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3301 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3361 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3421 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     3481 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     3541 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     3601 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3661 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3721 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     3781 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     3841 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     3901 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     3961 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     4021 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     4081 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     4141 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     4201 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     4261 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     4321 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     4381 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     4441 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     4501 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     4561 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     4621 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     4681 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     4741 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     4801 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     4861 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     4921 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     4981 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     5041 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     5101 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     5161 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     5221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5281 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     5341 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     5401 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     5461 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     5521 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5581 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     5641 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     5701 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     5761 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     5821 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     5881 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     5941 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6001 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6061 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6121 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     6181 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     6241 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6301 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6361 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6421 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     6481 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     6541 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     6601 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggtgat
     6661 cttcagacct ggacgatata tatgagggac aattggagaa gtgaattata taaatataaa
     6721 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     6781 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     6841 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     6901 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     6961 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     7021 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     7081 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     7141 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     7201 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     7261 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     7321 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     7381 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     7441 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     7501 ttagtgaacg gatctcgacg gtcgccaaat ggcagtattc atccacaatt ttaaaagaaa
     7561 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     7621 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     7681 ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcctgc agccccgata
     7741 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     7801 tttggcaagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga
     7861 atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga
     7921 tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt
     7981 tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt
     8041 ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct
     8101 tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag
     8161 ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg
     8221 cccggggggg atctggagct ctcgagaatt ctcacgcgtg ccaccatgag cgagctgatt
     8281 aaggagaaca tgcacatgaa gctatacatg gagggcaccg tggacaacca tcacttcaag
     8341 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
     8401 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc
     8461 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct
     8521 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     8581 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     8641 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag
     8701 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg
     8761 ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag
     8821 aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc
     8881 aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct
     8941 agcaaactgg ggcacaaact taatggtggt ggtggcgcgg cctctcggtc cggtcccaag
     9001 aagaagcgca aggtgggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     9061 gtggaggaga accctggacc tactagtgaa aagtttgctc ctgaattcca tggagaagat
     9121 gcaaacaaca gggctactaa attcctagaa tcaataaagg gcaaattcac atcacccaaa
     9181 gatcccaaga aaaaagatag tatcatatct gtcaactcaa tagatataga agtaaccaaa
     9241 gaaagcccta taacatcaaa ttcaactatt atcaacccaa caaatgagac agatgatact
     9301 gcagggggat ctggttcagg aggctctcgc ttggaagaag agttgcgaag aaggctcacc
     9361 gaaggatctg gttcaggagg caacaagccc aattatcaaa gaaaacctct agtaagtttc
     9421 aaagaagacc ctacaccaag tgataatccc ttttctaaac tatacaaaga aaccatagaa
     9481 acatttgata acaatgaaga agaatccagc tattcatacg aagaaataaa tgatcagaca
     9541 aacgataata taacagcaag attagatagg attgatgaaa aattaagtga aatactagga
     9601 atgcttcaca cattagtagt ggcaagtgca ggacctacat ctgctcggga tggtataaga
     9661 gatgccatgg ttggtttaag agaagaaatg atagaaaaaa tcagaactga agcattaatg
     9721 accaatgaca gattagaagc tatggcaaga ctcaggaatg aggaaagtga aaagatggca
     9781 aaagacacat cagatgaagt gtctctcaat ccaacatcag agaaattgaa caacctattg
     9841 gaagggaatg atagtgacaa tgatctatca cttgaagatt tctgaggtac c
//
