LOCUS       CMVpuro-STT3A-Q260R-HiBIT                10215 bp    DNA     circular SYN 29-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     misc_feature    4010..4598
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        2877..3376
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        27..330
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     LTR             5181..5438
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    9344..9577
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        331..534
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    7741..7875
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    8722..8847
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    10069..10184
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             666..2780
                     /note="pLannotate"
                     /label="STT3A"
                     /database="swissprot"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(7589..7693)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(6728..7588)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     LTR             8043..8671
                     /note="pLannotate"
                     /label="3' LTR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.2"
                     /fragment="False"
                     /other="LTR"
     CDS             3397..3996
                     /note="pLannotate"
                     /label="PuroR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(5969..6557)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(5615..5645)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(5660..5681)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             4615..5133
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="98.3"
                     /match_length="84.0"
                     /fragment="True"
                     /other="CDS"
     protein_bind    complement(5591..5607)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             8831..9169
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="22.6"
                     /fragment="True"
                     /other="CDS"
     CDS             9954..10054
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    619..650
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5694..5786)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gtttatcgat aagcttggga gttccgcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgactctact agaggatcgc tagcgctacc ggactcagat ctcgagctca tcgggatccg
      661 ccaccatgac taagtttgga tttttgcgat tgtcctatga gaagcaggac acacttttga
      721 agcttctcat tctgtcaatg gctgctgtat tatccttctc cactcgtctg tttgctgtcc
      781 tgagatttga aagtgttatc catgagtttg atccgtactt taattatcgg actaccaggt
      841 tcctggctga ggaggggttt tataaattcc ataactggtt tgatgaccga gcctggtacc
      901 ctttgggacg aatcattgga ggaacaattt acccaggttt aatgatcacc tctgctgcaa
      961 tctaccatgt actccatttt ttccacatca ccatcgacat tcggaatgtc tgtgtgttcc
     1021 tggcccctct cttctcctcc ttcacaacga tcgtcacgta ccaccttacc aaagagctca
     1081 aggatgcagg ggctgggctt cttgctgctg ccatgattgc tgtagttcct ggatatatct
     1141 cccgatctgt ggctggctcc tatgataatg aagggattgc catcttttgc atgctactca
     1201 cctactacat gtggatcaag gcagtaaaga ctggttccat ctgttgggca gctaagtgtg
     1261 cccttgctta tttctacatg gtctcgtcat ggggaggtta tgtgttcctg atcaacttaa
     1321 ttcctctcca cgtcctcgtg ctgatgctca caggccgttt ctctcaccgg atctatgtgg
     1381 cctactgtac tgtttactgc ctgggcacta tactttctat gcagatctcc tttgtgggtt
     1441 tccggcctgt cctttcatca gagcacatgg cagcctttgg ggtctttggt ctctgccaga
     1501 tccatgcctt tgtggattac ctgcgcagca agttgaatcc acaacaattt gaagttcttt
     1561 tccggagcgt catctctctg gtaggctttg tccttctcac cgtgggagct ctcctcatgc
     1621 tgacaggaaa aatatctccc tggacggggc gtttctactc gctgctagat ccctcttatg
     1681 ctaagaacaa catccccatc attgcttctg tgtctgagca tcagcccaca acctggtcct
     1741 catactattt tgacctgcag ctactcgtct tcatgtttcc agttggtctg tattactgct
     1801 ttagcaacct gtctgatgcc cggattttta tcatcatgta tggtgtgacc agcatgtact
     1861 tttcagctgt aatggtgcgt ctaatgctag tgttggcacc tgttatgtgc attctctctg
     1921 gcattggagt ctcccaggtg ctgtccacat acatgaagaa tctggacata agtcgtccag
     1981 acaagaagag caagaagcaa caggattcca cctaccctat taagaatgaa gtggcaagtg
     2041 ggatgatact ggtcatggct ttctttctca tcacctacac ctttcattca acctgggtga
     2101 ccagtgaggc ctactcttct ccgtccattg tactatctgc ccgtggtggg gatggcagta
     2161 ggatcatatt tgatgacttc cgagaagcat attattggct tcgtcataat actccagagg
     2221 atgcgaaggt catgtcctgg tgggattatg gctatcagat tacagctatg gcaaaccgaa
     2281 caattttagt ggacaataac acatggaata atacccatat ttctcgagta gggcaggcaa
     2341 tggcgtccac agaggaaaaa gcctatgaga tcatgaggga gctcgatgtc agctatgtgc
     2401 tggtcatttt tggaggcctc actgggtatt cctctgatga tatcaacaag tttctttgga
     2461 tggtccggat tggagggagc acagatacag gcaaacatat caaggagaat gactattata
     2521 ctccaactgg ggagttccgt gtggaccgtg aaggttctcc agtgctgctc aactgcctca
     2581 tgtacaagat gtgttactat cgctttggac aggtttacac agaagccaag cgtcctccag
     2641 gctttgaccg tgtccgaaat gctgagattg ggaataaaga ctttgagctt gatgtcctgg
     2701 aggaagcata taccacagaa cattggctgg tcaggatata caaggtaaag gacctggata
     2761 atcgaggctt gtcaaggaca ggctcgggca gcggtggctc tggaggttct gtgagcggct
     2821 ggcggctgtt caagaagatt agctaagaat tccgacatgc tctagataat tctaccgggt
     2881 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     2941 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     3001 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     3061 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     3121 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     3181 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     3241 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     3301 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     3361 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg
     3421 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     3481 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     3541 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     3601 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     3661 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     3721 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     3781 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     3841 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     3901 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     3961 cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct
     4021 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4081 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4141 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4201 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4261 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4321 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4381 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4441 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4501 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4561 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     4621 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     4681 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     4741 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     4801 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     4861 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     4921 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     4981 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     5041 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     5101 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     5161 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     5221 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     5281 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5341 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5401 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     5461 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     5521 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     5581 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5641 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5701 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5761 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5821 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5881 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5941 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6001 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6061 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6121 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6181 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6241 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6301 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6361 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6421 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6481 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6541 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6601 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6661 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6721 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6781 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6841 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6901 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6961 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7021 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7081 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7141 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7201 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7261 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7321 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7381 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7441 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7501 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7561 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7621 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7681 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7741 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7801 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7861 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     7921 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     7981 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     8041 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     8101 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     8161 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     8221 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     8281 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     8341 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     8401 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     8461 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     8521 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8581 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8641 tcagaccctt ttagtcagtg tggaaaatct cagcagtggc gcccgaacag ggacttgaaa
     8701 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     8761 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     8821 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     8881 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     8941 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     9001 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     9061 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     9121 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     9181 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     9241 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     9301 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     9361 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     9421 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     9481 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     9541 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     9601 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     9661 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     9721 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     9781 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     9841 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     9901 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     9961 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
    10021 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgcctt taaaagaaaa
    10081 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    10141 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
    10201 gacagcagag atcca
//
