LOCUS       pPIC9K-A2AR-V229       10308 bp    DNA     circular SYN 02-SEP-2025
DEFINITION  Pichia pastoris multi-copy integrating vector for methanol-inducible
            expression of a secreted protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Thermo Fisher (Invitrogen)
  TITLE     Direct Submission
  JOURNAL   Exported Sep 4, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
COMMENT     Sequence Label: pPIC9K
FEATURES             Location/Qualifiers
     source          1..10308
                     /lab_host="Pichia pastoris"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             join(949..1005,1006..1203)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             1204..1227
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1264..1287
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQGA"
     CDS             1294..2262
                     /codon_start=1
                     /label=A2AR
                     /translation="MGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSL
                     AAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIR
                     IPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVAC
                     LFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTL
                     QKECHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVN
                     PFIYAYAIREFRQTFRKIIRSHVLRQQEPFKAHHHHHHHHHH"
     primer_bind     2139..2172
                     /label=R291A
     CDS             2233..2262
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     terminator      2353..2599
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(3012..5546)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(5960..6775)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    7154..7910
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     rep_origin      complement(8379..8967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9138..9929,9930..9998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9999..10103)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgaaaaa
     1201 agagactaca aggacgatga cgataagtct aacaacaaca acaacaacaa caacaacctt
     1261 ggcgaaaact tgtatttcca gggcgctccc atcatgggct cctcggtgta catcacggtg
     1321 gagctggcca ttgctgtgct ggccatcctg ggcaatgtgc tggtgtgctg ggccgtgtgg
     1381 ctcaacagca acctgcagaa cgtcaccaac tactttgtgg tgtcactggc ggcggccgac
     1441 atcgcagtgg gtgtgctcgc catccccttt gccatcacca tcagcaccgg gttctgcgct
     1501 gcctgccacg gctgcctctt cattgcctgc ttcgtcctgg tcctcacgca gagctccatc
     1561 ttcagtctcc tggccatcgc cattgaccgc tacattgcca tccgcatccc gctccggtac
     1621 aatggcttgg tgaccggcac gagggctaag ggcatcattg ccatctgctg ggtgctgtcg
     1681 tttgccatcg gcctgactcc catgctaggt tggaacaact gcggtcagcc aaaggagggc
     1741 aagaaccact cccagggctg cggggagggc caagtggcct gtctctttga ggatgtggtc
     1801 cccatgaact acatggtgta cttcaacttc tttgcctgtg tgctggtgcc cctgctgctc
     1861 atgctgggtg tctatttgcg gatcttcctg gcggcgcgac gacagctgaa gcagatggag
     1921 agccagcctc tgccggggga gcgggcacgg tccacactgc agaaggagtg ccatgctgcc
     1981 aagtcactgg ccatcattgt ggggctcttt gccctctgct ggctgcccct acacatcatc
     2041 aactgcttca ctttcttctg ccccgactgc agccacgccc ctctctggct catgtacctg
     2101 gccatcgtcc tctcccacac caattcggtt gtgaatccct tcatttacgc ctacgctatc
     2161 cgcgagttcc gccagacctt ccgcaagatc attcgcagcc acgtcctgag gcagcaagaa
     2221 cctttcaagg cacatcacca tcaccatcac catcaccatc actgagcggc cgcgaattaa
     2281 ttcgccttag acatgactgt tcctcagttc aagttgggca cttacgagaa gaccggtctt
     2341 gctagattct aatcaagagg atgtcagaat gccatttgcc tgagagatgc aggcttcatt
     2401 tttgatactt ttttatttgt aacctatata gtataggatt ttttttgtca ttttgtttct
     2461 tctcgtacga gcttgctcct gatcagccta tctcgcagct gatgaatatc ttgtggtagg
     2521 ggtttgggaa aatcattcga gtttgatgtt tttcttggta tttcccactc ctcttcagag
     2581 tacagaagat taagtgagaa gttcgtttgt gcaagcttat cgataagctt taatgcggta
     2641 gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc
     2701 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg
     2761 tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg
     2821 tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt
     2881 ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact
     2941 acgcgatcat ggcgaccaca cccgtcctgt ggatctatcg aatctaaatg taagttaaaa
     3001 tctctaaata attaaataag tcccagtttc tccatacgaa ccttaacagc attgcggtga
     3061 gcatctagac cttcaacagc agccagatcc atcactgctt ggccaatatg tttcagtccc
     3121 tcaggagtta cgtcttgtga agtgatgaac ttctggaagg ttgcagtgtt aactccgctg
     3181 tattgacggg catatccgta cgttggcaaa gtgtggttgg taccggagga gtaatctcca
     3241 caactctctg gagagtaggc accaacaaac acagatccag cgtgttgtac ttgatcaaca
     3301 taagaagaag cattctcgat ttgcaggatc aagtgttcag gagcgtactg attggacatt
     3361 tccaaagcct gctcgtaggt tgcaaccgat agggttgtag agtgtgcaat acacttgcgt
     3421 acaatttcaa cccttggcaa ctgcacagct tggttgtgaa cagcatcttc aattctggca
     3481 agctccttgt ctgtcatatc gacagccaac agaatcacct gggaatcaat accatgttca
     3541 gcttgagaca gaaggtctga ggcaacgaaa tctggatcag cgtatttatc agcaataact
     3601 agaacttcag aaggcccagc aggcatgtca atactacaca gggctgatgt gtcattttga
     3661 accatcatct tggcagcagt aacgaactgg tttcctggac caaatatttt gtcacactta
     3721 ggaacagttt ctgttccgta agccatagca gctactgcct gggcgcctcc tgctagcacg
     3781 atacacttag caccaacctt gtgggcaacg tagatgactt ctggggtaag ggtaccatcc
     3841 ttcttaggtg gagatgcaaa aacaatttct ttgcaaccag caactttggc aggaacaccc
     3901 agcatcaggg aagtggaagg cagaattgcg gttccaccag gaatatagag gccaactttc
     3961 tcaataggtc ttgcaaaacg agagcagact acaccagggc aagtctcaac ttgcaacgtc
     4021 tccgttagtt gagcttcatg gaatttcctg acgttatcta tagagagatc aatggctctc
     4081 ttaacgttat ctggcaattg cataagttcc tctgggaaag gagcttctaa cacaggtgtc
     4141 ttcaaagcga ctccatcaaa cttggcagtt agttctaaaa gggctttgtc accattttga
     4201 cgaacattgt cgacaattgg tttgactaat tccataatct gttccgtttt ctggatagga
     4261 cgacgaaggg catcttcaat ttcttgtgag gaggccttag aaacgtcaat tttgcacaat
     4321 tcaatacgac cttcagaagg gacttcttta ggtttggatt cttctttagg ttgttccttg
     4381 gtgtatcctg gcttggcatc tcctttcctt ctagtgacct ttagggactt catatccagg
     4441 tttctctcca cctcgtccaa cgtcacaccg tacttggcac atctaactaa tgcaaaataa
     4501 aataagtcag cacattccca ggctatatct tccttggatt tagcttctgc aagttcatca
     4561 gcttcctccc taattttagc gttcaacaaa acttcgtcgt caaataaccg tttggtataa
     4621 gaaccttctg gagcattgct cttacgatcc cacaaggtgg cttccatggc tctaagaccc
     4681 tttgattggc caaaacagga agtgcgttcc aagtgacaga aaccaacacc tgtttgttca
     4741 accacaaatt tcaagcagtc tccatcacaa tccaattcga tacccagcaa cttttgagtt
     4801 gctccagatg tagcaccttt ataccacaaa ccgtgacgac gagattggta gactccagtt
     4861 tgtgtcctta tagcctccgg aatagacttt ttggacgagt acaccaggcc caacgagtaa
     4921 ttagaagagt cagccaccaa agtagtgaat agaccatcgg ggcggtcagt agtcaaagac
     4981 gccaacaaaa tttcactgac agggaacttt ttgacatctt cagaaagttc gtattcagta
     5041 gtcaattgcc gagcatcaat aatggggatt ataccagaag caacagtgga agtcacatct
     5101 accaactttg cggtctcaga aaaagcataa acagttctac taccgccatt agtgaaactt
     5161 ttcaaatcgc ccagtggaga agaaaaaggc acagcgatac tagcattagc gggcaaggat
     5221 gcaactttat caaccagggt cctatagata accctagcgc ctgggatcat cctttggaca
     5281 actctttctg ccaaatctag gtccaaaatc acttcattga taccattatt gtacaacttg
     5341 agcaagttgt cgatcagctc ctcaaattgg tcctctgtaa cggatgactc aacttgcaca
     5401 ttaacttgaa gctcagtcga ttgagtgaac ttgatcaggt tgtgcagctg gtcagcagca
     5461 tagggaaaca cggcttttcc taccaaactc aaggaattat caaactctgc aacacttgcg
     5521 tatgcaggta gcaagggaaa tgtcatactt gaagtcggac agtgagtgta gtcttgagaa
     5581 attctgaagc cgtattttta ttatcagtga gtcagtcatc aggagatcct ctacgccgga
     5641 cgcatcgtgg ccgacctgca gggggggggg gggcgctgag gtctgcctcg tgaagaaggt
     5701 gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca
     5761 cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc
     5821 acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt
     5881 cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca
     5941 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     6001 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     6061 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     6121 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     6181 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc
     6241 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     6301 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     6361 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     6421 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     6481 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     6541 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     6601 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     6661 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     6721 tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac
     6781 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     6841 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttccccc ccccccctgc
     6901 aggtcggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     6961 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     7021 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     7081 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     7141 ataagggaga gcgtcgagta tctatgattg gaagtatggg aatggtgata cccgcattct
     7201 tcagtgtctt gaggtctcct atcagattat gcccaactaa agcaaccgga ggaggagatt
     7261 tcatggtaaa tttctctgac ttttggtcat cagtagactc gaactgtgag actatctcgg
     7321 ttatgacagc agaaatgtcc ttcttggaga cagtaaatga agtcccacca ataaagaaat
     7381 ccttgttatc aggaacaaac ttcttgtttc gaactttttc ggtgccttga actataaaat
     7441 gtagagtgga tatgtcgggt aggaatggag cgggcaaatg cttaccttct ggaccttcaa
     7501 gaggtatgta gggtttgtag atactgatgc caacttcagt gacaacgttg ctatttcgtt
     7561 caaaccattc cgaatccaga gaaatcaaag ttgtttgtct actattgatc caagccagtg
     7621 cggtcttgaa actgacaata gtgtgctcgt gttttgaggt catctttgta tgaataaatc
     7681 tagtctttga tctaaataat cttgacgagc caaggcgata aatacccaaa tctaaaactc
     7741 ttttaaaacg ttaaaaggac aagtatgtct gcctgtatta aaccccaaat cagctcgtag
     7801 tctgatcctc atcaacttga ggggcactat cttgttttag agaaatttgc ggagatgcga
     7861 tatcgagaaa aaggtacgct gattttaaac gtgaaattta tctcaagatc tctgcctcgc
     7921 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     7981 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     8041 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     8101 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     8161 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     8221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     8581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9421 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     9481 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9541 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9601 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9661 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9721 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     9781 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9841 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9901 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9961 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10021 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10081 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    10141 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
    10201 caagaattaa ttctcatgtt tgacagctta tcatcgataa gctgactcat gttggtattg
    10261 tgaaatagac gcagatcggg aacactgaaa aataacagtt attattcg
//
