LOCUS       Exported                4031 bp ds-DNA     circular SYN 03-SEP-2025
DEFINITION  Addgene NGS Result.
ACCESSION   .
VERSION     .
KEYWORDS    pMB1-rop-Rj-YFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    403..542
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(644..835)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      1084..1113
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1258..1277
                     /label=DmR NT -15
     primer_bind     complement(1258..1276)
                     /label=DmR T -14
     primer_bind     complement(1260..1280)
                     /label=FndmR ctn -21
     primer_bind     complement(1264..1280)
                     /label=Primer 2
     primer_bind     complement(1264..1280)
                     /label=FndmR ctn -25
     primer_bind     complement(1304..1327)
                     /label=FndmR ctn -40
     primer_bind     1309..1328
                     /label=DmR T-35
     primer_bind     complement(1310..1339)
                     /label=Primer 1
     primer_bind     complement(1310..1333)
                     /label=FndmR ctn -34
     primer_bind     1312..1335
                     /label=TP1
     primer_bind     1312..1335
                     /label=FndmR ttn -32
     primer_bind     complement(1316..1339)
                     /label=NTP1
     primer_bind     complement(1316..1339)
                     /label=FndmR ctn -28
     primer_bind     1319..1342
                     /label=Primer 2
     primer_bind     1319..1342
                     /label=FndmR ctn -25
     primer_bind     1323..1346
                     /label=FndmR ctn -21
     primer_bind     complement(1329..1348)
                     /label=DmR NT -15
     primer_bind     1330..1349
                     /label=DmR T -14
     primer_bind     complement(1344..1384)
                     /label=Random_BR2
     misc_feature    1364..1435
                     /label=sfGFP
     primer_bind     1364..1382
                     /label=9 ct-15aa
     primer_bind     1364..1380
                     /label=T1-1
     primer_bind     1364..1380
                     /label=18tt-14aa
     primer_bind     1364..1377
                     /label=8 ct-13aa
     misc_feature    1364..1370
                     /label=1
     primer_bind     complement(1365..1388)
                     /label=NT1-1
     primer_bind     1365..1386
                     /label=OVERLAP_F
     misc_feature    1379..1381
                     /label=2
     misc_feature    1389..1390
                     /label=2-2
     misc_feature    1392..1396
                     /label=PAM
     misc_feature    1397..1441
                     /label=GFP-T1
     CDS             1537..2256
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     1788..1811
                     /label=15ct-83aa-1
     primer_bind     1789..1812
                     /label=27tt-83aa
     primer_bind     1791..1814
                     /label=T4af
     primer_bind     complement(1799..1814)
                     /label=9 gg-86aa
     primer_bind     2120..2143
                     /label=T6-6
     primer_bind     2122..2145
                     /label=T6
     promoter        2525..2629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2630..3490
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(3661..4031,1..217)
                     /direction=RIGHT
                     /label=ColEI
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
       61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
      421 ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct
      481 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
      541 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      601 ccgcttacag acaagctgtg accttctccg ggagctgcat gtgtcagagg ttttcaccgt
      661 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
      721 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
      781 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
      841 cctccgtgta agggggattt ctgttcatcc ctgaattcgc atctagactg aactggccga
      901 taattgcaga cgaacggatt tactttacca agcgagctcg taaacttggt ctgacagctc
      961 tagctccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     1021 aagattactt cgcgtttgcc acctgacgtc taagaaaagg aatattcagc aatttgcccg
     1081 tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac gtaattagtt
     1141 agttagccct tagtgactcg aattcgaatt cctcgtcagt tccgcttcgc tacaggatcc
     1201 ctatctggag agtgcccgat tgggacccga ctttcgcagg agcctattac ggagccactg
     1261 atgctagcac tgtaccttgg gtcctttcct cacgatagaa gtttcaggtt gacggctagc
     1321 tcagtcctag gtacagtgct agcatcagca ggacgcactg accactggat ggtgatgtca
     1381 acggtcataa tttttcgtga tcgacagcaa caagtgagcg gacgcaacta atggtagctg
     1441 tcaccggatg tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa
     1501 ttttgtttaa tactagagaa agaggagaaa tactagatgg tgagcaaggg cgaggagctg
     1561 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1621 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1681 tgcaccacag gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     1741 ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc
     1801 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1861 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1921 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1981 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2041 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacacccca
     2101 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     2161 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2221 gggatcactc tcggcatgga cgagctgtac aagtaatagg ccaggcatca aataaaacga
     2281 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2341 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagtagcg
     2401 gccgctgcag gagtcactaa gggttagtta gttagattag cagaaagtca aaagcctccg
     2461 accggaggct tttgactaaa acttcccttg gggttatcat tggggctcac tcaaaggcgg
     2521 taatcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2581 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2641 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2701 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2761 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2821 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2881 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2941 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3001 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3061 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3121 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3181 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3241 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3301 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3361 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3421 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3481 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3541 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3781 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgagttagg ccaccacttc
     3841 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3901 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3961 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4021 tacaccgaac t
//
