LOCUS       pDD7211_pLann       10721 bp DNA     circular SYN 17-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..10721
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     CDS             31..75
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     CDS             223..323
                     /codon_start=1
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    373..490
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     promoter        572..783
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=97.2"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     intron          784..1722
                     /label=EF-1-alpha intron A
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
                     /note="color: #------"
     CDS             1743..1808
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 1808"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1743..1792
                     /codon_start=1
                     /label=3xFLAG (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=75.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="DYKDHDGDYKDHDIDY"
     CDS             1827..4688
                     /codon_start=1
                     /label=SMARCAL1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MSLPLTEEQRKKIEENRQKALARRAEKLLAEQHQRTSSGTSIAGN
                     PFQAKQGPSQNFPRESCKPVSHGVIFKQQNLSSSSNADQRPHDSHSFQAKGIWKKPEEM
                     PTACPGHSPRSQMALTGISPPLAQSPPEVPKQQLLSYELGQGHAQASPEIRFTPFANPT
                     HKPLAKPKSSQETPAHSSGQPPRDAKLEAKTAKASPSGQNISYIHSSSESVTPRTEGRL
                     QQKSGSSVQKGVNSQKGKCVRNGDRFQVLIGYNAELIAVFKTLPSKNYDPDTKTWNFSM
                     NDYSALMKAAQSLPTVNLQPLEWAYGSSESPSTSSEGQAGLPSAPSLSFVKGRCMLISR
                     AYFEADISYSQDLIALFKQMDSRRYDVKTRKWSFLLEEHSKLIAKVRCLPQVQLDPLPT
                     TLTLAFASQLKKTSLSLTPDVPEADLSEVDPKLVSNLMPFQRAGVNFAIAKGGRLLLAD
                     DMGLGKTIQAICIAAFYRKEWPLLVVVPSSVRFTWEQAFLRWLPSLSPDCINVVVTGKD
                     RLTAGLINIVSFDLLSKLEKQLKTPFKVVIIDESHFLKNSRTARCRAAMPVLKVAKRVI
                     LLSGTPAMSRPAELYTQIIAVKPTFFPQFHAFGLRYCDAKRMPWGWDYSGSSNLGELKL
                     LLEEAVMLRRLKSDVLSQLPAKQRKIVVIAPGRINARTRAALDAAAKEMTTKDKTKQQQ
                     KDALILFFNRTAEAKIPSVIEYILDLLESGREKFLVFAHHKVVLDAITQELERKHVQHI
                     RIDGSTSSAEQEDLCQQFQLSERHAVAVLSITAANMGLTFSSADLVVFAELFWNPGVLI
                     QAEDRVHRIGQTSSVGIHYLVAKGTADDYLWPLIQEKIKVLAEAGLSETNFSEMTESTD
                     YLYKDPKQQKIYDLFQKSFEKEGSDMELLEAAESFDPGSASGTSGSSSQNMGDTLDESS
                     LTASPQKKRRFEFFDNWDSFTSPL"
     misc_feature    4708..5294
                     /label=IRES2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             5283..5864
                     /codon_start=1
                     /label=sta
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=72.2"
                     /note="/match_length=102.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MATTMGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTAT
                     GDGFTLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYS
                     GWNRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRR
                     MGFTLCGLDTALYDGTASDGEQALYMSMPCP"
     CDS             5874..5927
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 5924"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5928..6110
                     /codon_start=1
                     /label=CD52
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MKRFLFLLLTISLLVMVQIQTGLSGQNDTSQTSSPSASSNISGGI
                     FLFFVANAIIHLFCFS"
     misc_feature    6126..6147
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=95.5"
                     /note="/match_length=20.4"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             6149..6649
                     /codon_start=1
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=95.8"
                     /note="/match_length=82.7"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="RDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPM
                     TYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLT
                     FGWCYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARE
                     LHPD"
     LTR             6656..6836
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4"
     rep_origin      complement(6898..7486)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             complement(7657..8517)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8518..8622)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(8681..8733)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: LEFT"
     enhancer        8888..9267
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #ffffff"
     promoter        9268..9467
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     promoter        9269..9467
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             9485..9665
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
                     /note="color: #ffe4c4"
     misc_feature    9712..9837
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             9821..10159
                     /codon_start=1
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVR"
     misc_feature    10334..10567
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
                     /note="color: #ffe4c4"
ORIGIN
        1 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
       61 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
      121 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
      181 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
      241 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
      301 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
      361 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      421 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      481 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag taccgggccc
      541 gctctagcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      601 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      661 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      721 tacccccgtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      781 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      841 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
      901 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
      961 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     1021 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     1081 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     1141 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     1201 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     1261 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     1321 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     1381 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     1441 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     1501 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     1561 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     1621 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     1681 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg agcggccgca
     1741 tggactacaa agaccatgac ggtgattata aagatcatga catcgactac aaagacgatg
     1801 acgacaaggc tagcggtagc ggtagcatgt ccttgcctct tacagaggag cagaggaaaa
     1861 agattgaaga gaatcgacaa aaggctctgg cccgcagagc tgagaagtta ttggcagaac
     1921 agcatcagag gactagctcg ggcacctcca ttgctggcaa cccattccag gccaagcaag
     1981 gcccatccca aaatttccca agggagtctt gtaagccagt gagccatggt gtcattttca
     2041 agcaacagaa tctcagtagc tcatctaatg ctgaccaaag acctcatgat tcccacagtt
     2101 ttcaggcaaa gggaatatgg aaaaagccag aagaaatgcc cacagcctgc ccaggccaca
     2161 gtccacgtag tcaaatggct ctcactggaa tctctcctcc cttggcacaa agtcctccag
     2221 aggtccctaa acaacagctc ttgagttatg agttaggtca aggtcatgct caggcttcac
     2281 ctgagatcag gttcacaccc tttgctaacc caactcataa gcctctggcc aaaccaaaga
     2341 gttcccaaga gacaccagct cattcctctg gacagcctcc cagggatgct aagttagagg
     2401 ccaagacagc aaaagcctcc ccttcggggc agaacatttc ttacatccat tctagctcag
     2461 agagtgtaac gcccaggaca gaaggaagac tccagcagaa gtcagggtcc tcagtccaaa
     2521 aaggagtgaa ctctcagaag ggaaagtgcg taaggaacgg cgatcgtttc caggtgttga
     2581 ttgggtacaa tgcagaactc attgcagtgt ttaagaccct gcccagcaag aattatgatc
     2641 ctgacaccaa gacgtggaac ttcagcatga atgactatag tgccctgatg aaagcagccc
     2701 agagcctccc cacggtcaac ctgcagcctc tggaatgggc ctatggcagc agcgagtcac
     2761 cctccaccag cagtgaggga caggccggcc ttccatcagc tccatccctt tcatttgtca
     2821 aagggcgatg catgctcatc tccagggcct acttcgaggc agacatcagt tattcacagg
     2881 accttattgc gctttttaaa cagatggatt ccagaagata tgatgtcaag accaggaagt
     2941 ggagctttct cttggaagag cacagtaaac taattgcaaa ggtgcgctgc ctcccacaag
     3001 ttcagctgga ccctctgccc acgactctca ccctggcgtt tgcttctcag ctcaagaaga
     3061 catctctcag tctcacgcca gatgtcccag aggcagacct ttctgaagtg gaccccaagc
     3121 tcgtgtctaa tctgatgccc tttcagagag ctggagtcaa ttttgccata gccaaaggag
     3181 gccgcctgct gctcgctgac gacatgggcc tggggaagac catccaagcc atctgcatcg
     3241 cagcctttta ccggaaggag tggccgctcc tggtggtggt gccatcctcc gtgcgcttca
     3301 cctgggagca ggccttcctt cggtggctgc catctctgag cccagattgc atcaacgtcg
     3361 tggtgactgg gaaggaccgc ctgacagctg gcctgatcaa cattgtcagc tttgaccttc
     3421 ttagcaagtt ggaaaaacag ctaaaaaccc cttttaaagt tgtcatcatt gatgaatctc
     3481 acttcctcaa aaacagtagg actgcccgct gtcgagcagc tatgccggtc ctaaaggttg
     3541 ccaagagggt gatcctgttg tcgggcacac cagccatgtc ccggcccgca gagctctaca
     3601 cgcagatcat cgcagtcaag ccaactttct tcccccagtt tcatgccttt ggacttcgct
     3661 actgtgatgc caaacggatg ccttgggggt gggactactc aggttcctcc aacctgggag
     3721 agctgaagct cctgctggag gaagcagtca tgctgcggcg cctcaagtcc gacgtccttt
     3781 cccagctgcc tgccaagcag cgcaagatag tggtgattgc cccaggacgg atcaatgcca
     3841 ggaccagagc tgccctggat gctgcagcca aggaaatgac caccaaggac aaaactaaac
     3901 agcagcagaa agatgccctc attctcttct tcaacagaac agctgaagct aaaatcccat
     3961 ctgtcattga atatatcttg gacctactgg aaagtggaag agagaagttt ttagtatttg
     4021 cacaccataa ggtggtcctg gacgcaatta cgcaagagct tgagagaaag cacgtgcagc
     4081 acatccgcat cgatggctcc acctcatcag ctgagcagga ggacctgtgc cagcagttcc
     4141 aactgtcgga gaggcatgct gtggccgtgc tgtccatcac cgctgccaat atgggcctca
     4201 ccttctcctc ggctgacctg gtggtgtttg ctgagctgtt ttggaaccca ggggtgctga
     4261 tccaggctga ggaccgcgtg caccgcattg gacagaccag ctccgtgggc attcactacc
     4321 tcgtggcaaa gggcacagct gatgactacc tttggcccct gattcaagag aagattaaag
     4381 ttctggcaga agccgggctt tctgagacca atttttcaga aatgacagaa tccactgatt
     4441 acctctacaa ggacccaaag cagcagaaga tctacgacct attccagaag tcctttgaga
     4501 aagaaggaag tgatatggag ctcctggaag cagcagagtc ctttgaccca ggaagtgctt
     4561 caggaacatc tggaagtagt tcccagaaca tgggagacac cctggatgaa agctcattga
     4621 cagccagtcc acagaagaaa aggagatttg aattttttga taactgggac agctttacgt
     4681 ctcccctgta acccgggcta ggatccgccc ctctccctcc ccccccccta acgttactgg
     4741 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     4801 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     4861 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     4921 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     4981 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     5041 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     5101 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     5161 tatgggatct gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa
     5221 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     5281 atatggccac aaccatgggc accaccctgg acgacacggc ttaccgatac cgcaccagtg
     5341 tccctgggga cgccgaggcc atcgaggcac tggatgggtc cttcaccacc gacaccgtct
     5401 tccgcgtcac cgccaccggg gacggcttca ccctgcggga ggtgccggtg gacccgcccc
     5461 tgaccaaggt gttccccgac gacgaatcgg acgacgaatc ggacgacggg gaggacggcg
     5521 acccggactc ccggacgttc gtcgcgtacg gggacgacgg cgacctggcg ggcttcgtgg
     5581 tcgtctcgta ctccggctgg aaccgccggc tgaccgtcga ggacattgag gtagcacccg
     5641 agcacagggg acacggggtg ggacgggcat tgatgggact ggctaccgag ttcgccagag
     5701 aacggggagc tggtcatctt tggctcgaag tcaccaacgt caacgcaccg gcgatccacg
     5761 cttaccggcg gatggggttc accctctgcg gcctggacac cgccctgtac gacggcaccg
     5821 cctcggacgg cgagcaggcg ctctacatgt ccatgccctg ccccggcagt ggcgagggca
     5881 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg aagcgcttcc
     5941 tcttcctcct actcaccatc agcctcctgg ttatggtaca gatacaaact ggactctcag
     6001 gacaaaacga caccagccaa accagcagcc cctcagcatc cagcaacata agcggaggca
     6061 ttttcctttt cttcgtggct aatgccataa tccacctctt ctgcttcagt tgaggcgcgc
     6121 caaatttatc gataccgtcg acttcgatcg agacctagaa aaacatggag caatcacaag
     6181 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6241 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6301 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6361 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6421 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     6481 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     6541 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     6601 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     6661 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6721 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6781 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7321 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7561 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7621 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7741 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7801 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8641 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     8701 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     8761 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     8821 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     8881 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     8941 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     9001 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     9061 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     9121 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     9181 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     9241 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     9301 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     9361 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     9421 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
     9481 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9541 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9601 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9661 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9721 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9781 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9841 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9901 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9961 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    10021 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    10081 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    10141 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
    10201 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    10261 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10321 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10381 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10441 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10501 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10561 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10621 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10681 gggacagaga aattaacaat tacacaagct taatacactc c
//
