LOCUS       Exported                4644 bp DNA     circular SYN 15-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..4644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..42
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    50..66
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: RIGHT"
     repeat_region   141..200
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.6"
                     /note="/other=repeat_region"
                     /note="color: #a6acb3"
     repeat_region   192..261
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=49.6"
                     /note="/other=repeat_region"
                     /note="color: #a6acb3"
     promoter        284..495
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             502..1782
                     /codon_start=1
                     /label=KCNJ2
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MGSVRTNRYSIVSSEEDGMKLATMAVANGFGNGKSKVHTRQQCRS
                     RFVKKDGHCNVQFINVGEKGQRYLADIFTTCVDIRWRWMLVIFCLAFVLSWLFFGCVFW
                     LIALLHGDLDASKEGKACVSEVNSFTAAFLFSIETQTTIGYGFRCVTDECPIAVFMVVF
                     QSIVGCIIDAFIIGAVMAKMAKPKKRNETLVFSHNAVIAMRDGKLCLMWRVGNLRKSHL
                     VEAHVRAQLLKSRITSEGEYIPLDQIDINVGFDSGIDRIFLVSPITIVHEIDEDSPLYD
                     LSKQDIDNADFEIVVILEGMVEATAMTTQCRSSYLANEILWGHRYEPVLFEEKHYYKVD
                     YSRFHKTYEVPNTPLCSARDLAEKKYILSNANSFCYENEVALTSKEEDDSENGVPESTS
                     TDTPPDIDLHNQASVPLEPRPLRRESEI"
     misc_feature    1785..1806
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=20.4"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    1836..1905
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=85.4"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3"
     repeat_region   1975..2080
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=75.2"
                     /note="/other=repeat_region"
                     /note="color: #a6acb3"
     CDS             2114..2271
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=90.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="IHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPN
                     SCAA*MA"
     misc_feature    2277..2376
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=70.9"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     CDS             complement(2478..2513)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="LARFGDDGENL"
     promoter        2608..2712
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2713..3573
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3744..4332
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             4515..4607
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    4620..4641
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: RIGHT"
ORIGIN
        1 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
       61 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctctc gagatctaga
      121 aagcttcccg gggggatctg ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
      181 gggcgaccaa aggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      241 actccatcac taggggttcc tggaggggtg gagtcgtgac ctagggcaga gcgcacatcg
      301 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg
      361 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
      421 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
      481 gccgccagaa cacagggatc catgggcagt gtgcgaacca accgctacag catcgtctct
      541 tcagaagaag acggtatgaa gttggccacc atggcagttg caaatggctt tgggaacggg
      601 aagagtaaag tccacacccg acaacagtgc aggagccgct ttgtgaagaa agatggccac
      661 tgtaatgttc agttcatcaa tgtgggtgag aaggggcaac ggtacctcgc agacatcttc
      721 accacgtgtg tggacattcg ctggcggtgg atgctggtta tcttctgcct ggctttcgtc
      781 ctgtcatggc tgttttttgg ctgtgtgttt tggttgatag ctctgctcca tggggacctg
      841 gatgcatcca aagagggcaa agcttgtgtg tccgaggtca acagcttcac ggctgccttc
      901 ctcttctcca ttgagaccca gacaaccata ggctatggtt tcagatgtgt cacggatgaa
      961 tgcccaattg ctgttttcat ggtggtgttc cagtcaatcg tgggctgcat catcgatgct
     1021 ttcatcattg gcgcagtcat ggccaagatg gcaaagccaa agaagagaaa cgagactctt
     1081 gtcttcagtc acaatgccgt gattgccatg agagacggca agctgtgttt gatgtggcga
     1141 gtgggcaatc ttcggaaaag ccacttggtg gaagctcatg ttcgagcaca gctcctcaaa
     1201 tccagaatta cttctgaagg ggagtatatc cctctggatc aaatagacat caatgttggg
     1261 tttgacagtg gaatcgatcg tatatttctg gtgtccccaa tcactatagt ccatgaaata
     1321 gatgaagaca gtcctttata tgatttgagt aaacaggaca ttgacaacgc agactttgaa
     1381 atcgtggtca tactggaagg catggtggaa gccactgcca tgacgacaca gtgccgtagc
     1441 tcttatctag caaatgaaat cctgtggggc caccgctatg agcctgtgct ctttgaagag
     1501 aagcactact acaaagtgga ctattccagg ttccacaaaa cttacgaagt ccccaacact
     1561 cccctttgta gtgccagaga cttagcagaa aagaaatata tcctctcaaa tgcaaattca
     1621 ttttgctatg aaaatgaagt tgccctcaca agcaaagagg aagacgacag tgaaaatgga
     1681 gttccagaaa gcactagtac ggacacgccc cctgacatag accttcacaa ccaggcaagt
     1741 gtacctctag agcccaggcc cttacggcga gagtcggaga tatagaattc gatatcaagc
     1801 ttatcggcgg ccgctaggcc tcacctgcga tctcgatgct ttatttgtga aatttgtgat
     1861 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1921 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaac tagtccactc
     1981 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     2041 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggacag atccgggccc
     2101 gcatgcgtcg acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     2161 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     2221 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     2281 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     2341 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     2401 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     2461 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     2521 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2581 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2941 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3001 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3061 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3121 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3181 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3241 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3301 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3361 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3421 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3481 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3541 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3601 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3661 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3721 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3781 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3841 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3901 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3961 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4021 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4081 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4141 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4201 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4261 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4321 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4381 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4441 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4501 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4561 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4621 aatgtgagtt agctcactca ttag
//
