LOCUS       pHNF4A-Enh_Gsta2       14259 bp    DNA     circular SYN 09-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             771..864
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.679593721144968"
                     /note="/other=CDS"
                     /translation="SAQYANRLSPRVGRFINAAGTTGFPTGKRAV"
     protein_bind    complement(877..898)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
     promoter        913..943
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(947..969)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    1031..3401
                     /label=H11 homologous arm
     misc_feature    complement(2002..2122)
                     /label=Rosa26 right arm
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=95.041"
                     /note="/match_length=2.78866098179304"
                     /note="/other=misc feature"
     misc_feature    3408..3426
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=17.59259259259259"
                     /note="/other=misc feature"
     polyA_signal    3426..3560
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.265"
                     /note="/match_length=100.0"
                     /note="/other=polyA signal"
     CDS             complement(3581..4300)
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(4319..4696)
                     /codon_start=1
                     /label=H2B
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     misc_feature    complement(4703..5289)
                     /label=IRES
                     /label=IRES2
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.83"
                     /note="/match_length=100.0"
                     /note="/other=misc feature"
     misc_feature    complement(5362..6027)
                     /label=Gsta2
     misc_feature    6028..6036
                     /label=Kozak sequence
     misc_feature    6039..6559
                     /label=Shh miniP
     misc_feature    6590..9862
                     /label=HNF4A Enhancer
     misc_feature    9863..11386
                     
     promoter        complement(11402..11420)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             12232..13026
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    13047..13103
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=98.246"
                     /note="/match_length=40.42553191489361"
                     /note="/other=misc feature"
     promoter        13124..13183
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=58.82352941176471"
                     /note="/other=promoter"
     CDS             complement(13276..14136)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14137..14189)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=73.61111111111111"
                     /note="/other=promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcat tgactgcagg ctgagtgagt
     1081 gggggtacgt aaggacccaa agctatgtag cctagcttct caaggtgtct aaagaaagga
     1141 agggtagatt accaatgagc agagtttggg cttctctatt tttaaagttt taaaatgttt
     1201 atatcaaggc aaggtttctg ttgtccttgc tggtctggaa ctagattggc cttgacctaa
     1261 gagatgatgc gacttctgcc tcccaagtgc taattaaagg tgtgagtcac cacgcttgcc
     1321 ttggtgtttt ctatttatag gttatatatt catttttcct attgcatgtc acttcccaaa
     1381 ttacatctca tctagtcatc tgtgcaccca atcacaaata ggcataacct ggtagatttc
     1441 cactaaaagt tacttagagg aagtttgtga atttattatt acaggcaagc catggaaatg
     1501 tgtgtagctg ttgcagttgg tttgaggagg tgtaagtgca tggtgtattt ggtagagaga
     1561 ctaggctgcc tagttcatta tgagaagata tgtacaacac aaacgaagta gggtctcagg
     1621 ttcaactgtt aagctttctt gcctcaggct gctggagagt tgcactcgta cttattcact
     1681 caattcattc tttggccagg tgggtggtgg tggtggtggt tcacaccttt agtcatagtg
     1741 ttgaggaggc agaggcaaga ggttctgtgt ttgaggccag ctgggtctat agattgaatt
     1801 ctaggaaagc cagggctaca tagagaaacc tgttttcaaa agaaaaaaga aaaaaagaaa
     1861 agacgaatgg tgtatgtgtg tgtttgtcag tcatctgtct ggaattgaat ttgagatccc
     1921 cctgcctccc aagtgtcctg attaaaggca tagtctatat agctgggagt ggtggcgcac
     1981 acttttaatt ctagcacttg agaggcagag gcaggcggat ctctgagttc gaggccagcc
     2041 tggtcaacaa agtgagttcc aggacagcca aggctacaca gagaaaccct gtctcgaaaa
     2101 aacccaaaaa aaaaaaaaaa aaaaaagagt aaaggcatag tctaccacat ccactgaggt
     2161 gtgttcttac agccaaagac tttttctcct gaggtctaag ggaacctcgt agtatgagtc
     2221 tcatgacatg gaatgaattt cctgttttgt tttcttggcc ttagttaagc taagcagacc
     2281 cactattctg caccactcat tagttaggat gctaacactc atttaagagg ttaatgattg
     2341 aaaaggcatt ttcacagaaa ccatatggcg ctccagtggc tttcattagg tcatttgaga
     2401 atccagattt tctcatcttc tctattcact acaggttgtt ggcagttttg gccagttagc
     2461 tcaccttgaa aatggaaaca tgtctgacaa gagccttgag ctgaatatca tccagagtat
     2521 ttgctagaga cagggtctta agtctcatta aattgcttag aagtgtgttt agtcctataa
     2581 actatgtctc atttgtgtgt ccttcacaaa gagcgctagt gtcgatcatc aattagccta
     2641 gcctataaag gtgacacaac ctggtcagtc cctctgtatg tctactattt ccccttctga
     2701 tttctactat cttctcaaga actggttctt cagcttcctt tgggaaatgt cactttttaa
     2761 tgatgtgtgt tttgcttatg tatatgtcta catactaccc atgtgcttgg tcactgcaga
     2821 ggccagaaga cggtgtcagg tgcactggaa ctagagttaa tgacagatgt gagccatagg
     2881 gtgctttgtt cacatctttc cagtcccaaa tgcgtctaaa cttatgtgat acatactaga
     2941 ttaccacaaa attgctccaa agacaccttt ctccctctga gatgaatctc tggctggcct
     3001 tgctctaggc aatcctgtgt tcaattcaag gactgaattt gattacatcg gtaatccagt
     3061 gccccaggca tttctgcttt ttctgtaagg ttcttatccc ctggaagact gtttacgtat
     3121 tcattttttt ttgagactaa gtttcaggta gaaaaagctt gccttgaact tcactatata
     3181 gggcttatct tgaactcttg tgggtcttcc acctttcttc agttagcttc tgtacactgc
     3241 cagacatgaa aatcagatcc atttatagat agatggtcat gagccaccat gtgggtgtct
     3301 aaaattgatc tcgggacctc tgaaagacca gctagttctc ttaactgctg agccatctct
     3361 ctagcgtgtc tatacacatt taatatcccc ttgttccctt tctgcttgcc gctctagaac
     3421 tagtggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3481 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3541 taagctgcaa taaacaagtt aacaacaact taacaacaac ttacttgtac agctcgtcca
     3601 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     3661 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
     3721 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     3781 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     3841 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     3901 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     3961 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     4021 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     4081 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     4141 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     4201 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     4261 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtggatcct
     4321 tagcgctggt gtacttggtg atggccttag taccctcgga cacggcgtgc ttggccaact
     4381 ccccaggcag cagcaggcgc acggccgtct ggatctccct ggaggtgatg gtcgagcgct
     4441 tgttgtaatg cgccaggcgg gaagcctcac ctgcgatgcg ctcgaaaatg tcgttcacaa
     4501 acgaattcat gatgcccatg gccttggacg aaatgccggt gtcagggtgg acctgcttca
     4561 gaaccttgta cacatagatg gaatagctct ccttgcggct gcgcttgcgc ttcttgccgc
     4621 ctttcttctg cgccttagtc accgccttct tggagccctt tttcggggcg ggagcagact
     4681 tcgctggctc tggcataacc atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa
     4741 accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta aacacatgta
     4801 aagcatgtgt accgaggccc cagatcagat cccatacaat ggggtacctt ctgggcatcc
     4861 ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac aactatccaa
     4921 ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt
     4981 tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag ggtcgctaca
     5041 gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac attcaacaga
     5101 ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg
     5161 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag
     5221 acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg ggggggggag
     5281 ggagaggggc ggatcccccg ggctgcagga attcgatatc aaccattggg atcggccagt
     5341 aacgttaggg ggggggaggg actaaaactt gaaaaccttc cttgcttctt caatttgttt
     5401 tgcatccaag ggaggctttc tctggctgcc aggatgtagg aacttcttca cattggggag
     5461 gctgctgatt ctgctcttga aggccttcag cagagggaaa ggggtcagaa ggctggcatc
     5521 aagctcttca acatagagaa gaagttccag caggtggacg tccaccctgg tcagcctgtt
     5581 gcccacaagg tagtcttgtc catggctctt caacaccttt tcaaaggcag gcaagtaacg
     5641 gtttttggtc ctgtcttttg ccaaggcagt cttggcttct ctttggtctg ggggacataa
     5701 taccaattgc ccaatcattt cagtcagatc taaaatacct tctgtataca tgtcaatcag
     5761 ggctctctcc ttcatgtcct tcccatagag gtcatatttg gtggcgatgt agttgagaat
     5821 ggctctggtc tgcaccagct tcatcccatc aatctccacc atgggcactt ggtcaaacat
     5881 caaattccca tcttttttta gcttttccaa atcttccgga ctctgtataa acttctcttc
     5941 aaactccacc cctgctgcag ccaggagcca cctgatgcac tccattctgc cccgggcatt
     6001 gaagtagtga agcacgggct tcccggccat ggtggcatct cgtccgcgga acctgaggac
     6061 ttgtgagctg tccccgcgct gtctgtgcgc gagcgggtac gcgcgggtgt gtgcgtgtgc
     6121 gctcctcctt gcgctcggct ctcccttcct cgctcggctc gttcgttccc tctggcgctg
     6181 cctggctctt tctcttccta tataaccttg cctgccgctg ctgcagggga cttgtctccg
     6241 atccccaccc agactggggc tgcgatggca ggctgccggt cgctggtaac ggaacacatc
     6301 ggagttgggt ctcgagacag caattaaaag acaaaaagag cctgatttta aatggtagca
     6361 aggctggaga gcttgtgaga caggctgcct ttcgcactat attatagctg gcaggggcgc
     6421 atctgattgg ccaagccgca caagcttgtc ttctgatgtt aaccctgaga aagactacat
     6481 ttctcccccc tctggctgct gtggctttat tttcacctga aggcttccag tttaaaaaag
     6541 aaagaaagag agaaagaaag gctgctcagt ttggatgttc ctggcggcca atcccagttc
     6601 tgtcgctaac ttgctctgtg acttcaggca aatttctgtc tctctctaag ccccaatctc
     6661 tttctgcaat tgtatccagg cccaagaagc acaccaactg ggaatctagt ttctggggca
     6721 atgtcaacaa aattgggaag ccctttggag gacaatgccc ccgactttcc cagggatgcc
     6781 ccacctgcat tgggcaagct gggctcctca tataccagga atggggagct gggaaccctt
     6841 ctgatcccag gttcaacccc ctgatatggc agctgttctg gggggtgggc cgggaggtcg
     6901 gggtggtgtg ttaatgattg tcctgtgtta ttggccaccc atcattgctc tctgagaatg
     6961 ctctgagccc cagccaggga ggtttgggtg ggaaatcgag gttaacaatt gctcaggttg
     7021 ggggtatctt gttgccctgc taagcccctg ggaaggaaca ggagggatta aacattaatg
     7081 tagagactcc agcagagggg actggggcca gagcacaagg aacaggggag agggtagggg
     7141 cggattgtcc aagatccttg ccctgtcgac tctgctaatg gaaggaggaa ccatggaaga
     7201 gggccccggg tccagcccct gtatgaggct ggggacaagc caaaccagcg ttttcacagt
     7261 gtcccaatgt gcagtattaa taggtgttcc aggagaatct tgccgaagga gtttgagaag
     7321 cctcggcctc tgcaaagtca aacaactgcc tggacgagac ttctcagaag ctttcacaga
     7381 ctcacatgca ttgtcagtgt cttatgcaaa ggtgcgaaac gggtaagcga atgcaccaga
     7441 gtctgtctcc gaagctgaga agttgcaggg gaactcaagg acctgagaag taaagcacct
     7501 tatcccgggg tataggcagg ggcaggggca tcctccacac gcctggccac cccagggctg
     7561 cttggcatct tcacttcccc ttggcgctca ccacaccatt atctcctatc ttttcttccc
     7621 cacccaccac tccgggagag gtgcagagaa aactgggact tatcaagaca aagaacaaaa
     7681 gtcgtggagg aaagaagcca agagccatct ctactctggg gtagggccct tcagttttgc
     7741 ccctttgaaa tttcaaattc cagtttgtgg acaaagtcct aactatctca caatataggt
     7801 ccccaaccac tgaccaaact ccagtccagg cagccaccag ctggcctggt cttgctgctt
     7861 cctttagcgg cttccaaggt ccagggacag ggggtctggg ccaccaagag gctctgctag
     7921 gctgtccctg cagccacagc caccccactg gacccctgcc tcccatctga gaggacagcc
     7981 ctcttcctgg agctgggatc tgacacttgc tgataccaac ggcagatacc aggtaggtcc
     8041 cctccctttc tcttccttga gctcctaaaa tgccactcat accaccctct gcactcggca
     8101 aaggccactg tggcttaatc accatagact tggggcccag acctggggcc cctcacggag
     8161 cctcagaggg tgaaagggac ttgctcaagt ccacgcagcg gtggtgggtg agctttgtct
     8221 taacaacgag cactttctac ttcatcacag accatgagtg gcattgtgtt tgttcacccg
     8281 atgatgggtg gccactggct tgttcacccg atgatgggtg gccactggct tgtcacagga
     8341 gtatttccga gaggaagatg agggagcttg gggctgaagg ccagtgggtt tggaaggaag
     8401 caacattcat gtggttaact gataactggg ccccatccct gggcctcctt cctccctccc
     8461 tgtccttagc ctgattaaga ccaacttacc cagctgctaa tcattgctga gcctgttgat
     8521 tttgccttaa aaaattaact gggcatgagt ttgcggtttt cctcacccct aaccctgaaa
     8581 aggcctgggt gggcaggagg cctccagggt tatgcaagag gccgctggcc tcccaacgca
     8641 tagcccacgg ccacctcggc tgctctccaa agaagaggct ttgctcaaga gtcaacagtc
     8701 tgcttgttcc ccctgtgctg ggtcctgagc aaatttgctc tgttaccaaa gagagataaa
     8761 ggcagctggg ggagagcttt ccaaacaggg atgaccagag gcacagggag gttgagccat
     8821 atacccatgc tcacacagct gatgagaaac aagaagtgga attggaactt gggcagtctg
     8881 tctcaacagt ctgtgcctct aactccaggg ctacccttcc cttccaggga tgccagtgag
     8941 agatggccag gagaagggca gagagcaggg cactgtggga gatgctgact gtaaggctcc
     9001 ggaggtaggg cagagagtat ggtctggaga atgatcagga aggaagccag gaggacttgg
     9061 atctgtgtgg gtggagcttc tccgcaggct gcctcctgcc aggtggctta tgacatctgg
     9121 gctgagggtg ttgctgtcct cctgtttatg ttcatccctc ctgtttatgt tcctgaattt
     9181 atctgcaggg gtgaccttgg gaattagatg cggccttccc ggcccttgtg gggcctgaag
     9241 gctacctggg gatgggcagg acgatcccct gagctagtgg cctttgcctg cacaggtcat
     9301 tttagctcca atgagatgcc actttgcaaa agttctttga ggatcttaaa gggcacggtg
     9361 gaggctgcta aacttgagag aaggactctg aggagggcaa ggggcgcacc tgagggagtg
     9421 gggctgctaa gtctcacttg atagactcca cagttttgca acttggatct gggccttcac
     9481 aaaccttcgc gaacccgcag ccccacccct cgggtcagcc aatgtcttct gtggggctgg
     9541 gagccagggg aagaggccaa aggaactgtg cagagaagcg tggccacccg ggccgcagct
     9601 gggagccctg acaccctttg ccgccccacc tcatcccgcg gttgccccag ggcgcagggc
     9661 agggcaggca ccgcgctggg ccggagggcg tcccggagga ggcggccaag actctccgca
     9721 gtgctgcgct ttgcgcttcc tgggctcctc ctcgtggcca acgcaggaac tggtgttcag
     9781 aaacttagat agccttaggg acttcaccaa tcacagcaat cccgccaatc acaggcccag
     9841 acgcactatg tctctccaaa tcgtggcata tgtaactttt aataaaccaa aagaaaaagt
     9901 cccaaatatt caagtgaaaa aaatccaaac agttgacaca agcctaactg atagttactt
     9961 tatgtacagc tgtatgtata agttcaaaaa aagctaccct atttgtatgt acaaaagttt
    10021 atacacaggt ctgtacataa gggtctatac attttatttt ttcagaaccc ttaggtgtca
    10081 cctctagaag acaccaacac ttcattcaca tattttataa aagaaagact tccaggactg
    10141 acaatcttgt atcccttgta tttgaaccat gtaggttcat tcgatttaac agccttctgt
    10201 cataatgtag atcatggtag gtatgtggat cccctaaaca tcttcacata gtgtgctcca
    10261 atgtaaaagc ccattacaaa gagggaatga gagtcatgac cagggtgtca ccacaggtcc
    10321 aggtatgggt gagccagcct gccctcttca ctgtctatat tccagttcat ctacactgat
    10381 gactttggtg ggcatggagg gcagaggctg ctgtagcaca tctgagccaa accatttggc
    10441 aaggcccaca ggggagctgc tcctttggct ggtacgatgc tccagctggg agtgcatgtg
    10501 gggcatgccc gaccggctgg gtacattctg aggagtctga acgctgatag ctgctgcctg
    10561 ggaactagag cctggaggat gcagaactat ggagagaaga gtgtcagtgc agcaaagtag
    10621 ctctactcta cacctcaccc acagaacccc acaagtctac taagaccacc cttggatact
    10681 cactcccaag tcctgttctc agtattgaca atattttctg acctactttg cttgaatggt
    10741 ttgttacttt gggggatggg aagtctacca gaggatttaa ccaaaggctt cctaaagtgc
    10801 aaaatgcaac gtaagaaaat gcaacgtaag aaagcccacc aaggctgaca tgttcacatc
    10861 tcagcaaaaa ttctcaggct tataagaagt gaaaaacaga gctagagcat ggctggctcc
    10921 gactgtcctt gcagtagtaa taatccctga gactgcacag gtcagttcac ttcacctgtt
    10981 tttctagtga cacctaagaa acaaaggggc agtgataact attgctcctg gaaatctcag
    11041 aactttccag tgtgatcagc taacctggcc tatttctaaa ccttacctga gcgctgtagc
    11101 tgctgctgca tcaccgccaa atgcagaggg gtcccaggac gagggtttag gagagggtga
    11161 ctagcagcag agaccaaagg gtacaagggt tgacccagga gtgggccaga tagcccctgt
    11221 aaaggagcca tgtctatccc aggaggaagc acacctgatg gggagaaaga agaaactcat
    11281 gagttgtaga tgttcctaaa aatgtcaaag tttactactt ttgttgattt ggatacttac
    11341 aaggaggtgg atgtggtgaa cacttgccac catacatgta ccagggctaa attcaattcg
    11401 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
    11461 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    11521 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct atacgtacgg
    11581 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
    11641 agtgatatta ttgacacgcc ggggcgacgg atggtgatcc ccctggccag tgcacgtctg
    11701 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
    11761 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
    11821 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
    11881 taaatgtcag gcatgagatt atcaaaaagg atcttcacct agatcctttt cacgtagaaa
    11941 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca
    12001 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag
    12061 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct
    12121 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga
    12181 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa
    12241 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
    12301 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
    12361 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
    12421 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
    12481 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
    12541 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
    12601 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
    12661 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
    12721 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
    12781 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
    12841 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
    12901 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
    12961 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
    13021 ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
    13081 ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    13141 tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa aaaggatctt
    13201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    13261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    13321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    13381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    13441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    13501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    13561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    13621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    13681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    13741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    14041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    14101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    14161 aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt tttcgttcca
    14221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
