LOCUS       Exported                5619 bp DNA     circular SYN 20-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..5619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(334..420)
                     /label=rrnB T1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
                     /note="color: #ffffff; direction: LEFT"
     CDS             complement(432..1844)
                     /codon_start=1
                     /label=AS
                     /note="color: #993366"
                     /translation="MEHTPHIAMVPTPGMGHLIPLVEFAKRLVLRHNFGVTFIIPTDGP
                     LPKAQKSFLDALPAGVNYVLLPPVSFDDLPADVRIETRICLTITRSLPFVRDAVKTLLA
                     TTKLAALVVDLFGTDAFDVAIEFKVSPYIFYPTTAMCLSLFFHLPKLDQMVSCEYRDVP
                     EPLQIPGCIPIHGKDFLDPAQDRKNDAYKCLLHQAKRYRLAEGIMVNTFNDLEPGPLKA
                     LQEEDQGKPPVYPIGPLIRADSSSKVDDCECLKWLDDQPRGSVLFISFGSGGAVSHNQF
                     IELALGLEMSEQRFLWVVRSPNDKIANATYFSIQNQNDALAYLPEGFLERTKGRCLLVP
                     SWAPQTEILSHGSTGGFLTHCGWNSILESVVNGVPLIAWPLYAEQKMNAVMLTEGLKVA
                     LRPKAGENGLIGRVEIANAVKGLMEGEEGKKFRSTMKDLKDAASRALSDDGSSTKALAE
                     LACKWENKISST"
     CDS             complement(1863..2360)
                     /codon_start=1
                     /label=ubiC
                     /note="color: #993366"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGKT
                     VSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSGPE
                     LALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLPASP
                     LY"
     CDS             complement(2379..3815)
                     /codon_start=1
                     /label=MNX1
                     /note="color: #993366"
                     /translation="MAVQAPSKTYGFQKAPIQLTFVVVGAGLGGVAASICLRLAGHRVI
                     LLEAAELGEVGAGIQIPPPSTKILKAIGVLDAVDKVSIHPHDILVKKYKGELLSTQNLV
                     PYVLEKYDGMYLHIHRADYHKVLVDRAEELGVEIHTNSRVVDIDFEKATVTTATGKQYS
                     GDVIVGYDGVRSQTRALLTGDSSGAYDTGDLAYRALIKVEDMKKVPGLEKFYANPNINF
                     WWGPTMHIVMYFLHEGEICNVVALCPDTLPKGVLKQDASQEELLDLVKGWDQDLTTVFK
                     LITSVSKWRLQDSRELKTWVNSKTGNFIILGDASHSTLPYLASGASQAVEDGAVLAGLF
                     SKIELRDQIPQLLQMTENLRKWRSSQVVRGSHQCQDIYHLPDGELQEIRDSYLYDKQPE
                     LGCPNRFADPVFQDFLWGYNAFDEVERAWKEFKAGGNPTYTYPNLYKPKSSGEKDVSGG
                     GAAATLAAGNTPAAPLSASG"
     promoter        complement(3843..3875)
                     /label=PLtetO-1 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=44.6"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    complement(3875..3891)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(3898..3914)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b; direction: LEFT"
     promoter        4023..4127
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             4128..4988
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      5018..5112
                     /label=lambda t0 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
                     /note="color: #ffffff"
     rep_origin      join(5200..5619,1..169)
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
ORIGIN
        1 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
       61 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      121 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      181 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      241 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      301 ccgcagccga acgccctagg tctagggcgg cggatttgtc ctactcagga gagcgttcac
      361 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      421 atgcctctag attaggtaga ggagattttg ttttcccatt tgcacgccag ttccgccagt
      481 gctttggtgg aagaaccgtc gtcagacagc gcacgggatg ccgcgtcttt caggtctttc
      541 atggtagagc ggaatttttt gccttcttcg ccttccatca ggcctttaac cgcgttcgcg
      601 atttctacgc ggccgatcag gccgttttca cccgctttcg gacgcagcgc cactttcaga
      661 ccttcagtca gcattaccgc gttcattttc tgttctgcgt acagcggcca cgcgatcagc
      721 ggcacaccgt taactacaga ttccaggata gagttccaac cgcagtgggt caggaagcca
      781 ccagtggaac cgtggctcag gatttcagtc tgcggcgccc aagaagggac cagcagacaa
      841 cgacctttgg tacgttccag gaagccttcc ggcaggtatg ccagagcgtc gttctggttt
      901 tggatgctga agtacgtggc attggcaatc ttgtcattcg ggctgcgcac aacccacagg
      961 aaacgttgtt cgctcatttc caggcccaga gccagttcaa tgaactgatt atgggacact
     1021 gcaccaccgc tgccgaaaga aatgaacaga acggaaccac gcggctggtc atccagccat
     1081 ttcagacatt cacagtcgtc aactttgctg ctggaatccg cacggatcag cgggccgatc
     1141 gggtaaaccg gcggtttacc ctggtcttcc tcttgcagag ctttcagcgg gcccggctcc
     1201 aggtcgttga aggtgttaac catgataccc tcagccaggc ggtaacgttt ggcctggtgc
     1261 agcaggcatt tgtaggcatc gtttttgcga tcctgagctg ggtccaggaa atccttaccg
     1321 tgaatcggga tgcagcctgg aatctgcagt ggttctggga cgtcacggta ttcgcaggac
     1381 accatctgat ccagcttcgg caggtgaaag aacagagaca ggcacatggc cgtcgtcgga
     1441 tagaagatat aaggggagac cttgaactca attgcaacat caaatgcatc ggtgccaaac
     1501 aggtccacca ccagagcagc cagcttggtg gtggccagca gagtcttaac tgcatcacga
     1561 acaaacggca gagagcgagt gatggtcaga caaatacggg tctcgatacg aacatcagcc
     1621 ggcaggtcat caaagctgac cggcggcagc agaacatagt ttacgcccgc cggcagagca
     1681 tccagaaaag atttctgtgc tttaggcaga ggaccatcgg ttgggataat gaaagttacg
     1741 ccgaagttgt gacgcagtac caggcgttta gcgaactcaa ccagcgggat cagatgaccc
     1801 atacccggag tcggcaccat agcgatgtgc ggggtatgct ccatggtata tctcctgcat
     1861 gcttagtaca acggtgacgc cggtaaaaac agttctgtta gcaacagcgg tttaccgctt
     1921 aatcgcaggc gggaacgtcg cccccacagc ccggcatcac ggcctatctc aataaagtcc
     1981 cgggttaatg tcgatgatgt gaacagatag cgtcctaacg gcgttttacc caatttttgt
     2041 aacgccagct ccggcccgct taacgttgac acaggaacga cggtacgacc ggcaagccac
     2101 ggttcaccat cggcacataa caaaatttca cgtaaccagt aacgagactc tttcggcagc
     2161 agcggcagtt cttcggggat ttcattctgc tcgacaaacc cttcgcggat catcgtcacg
     2221 cttaccgttt ttccctgctg ttcaaaacgt tttgtcatgg aatcctccag caacagccag
     2281 tcgagcagtt gcggatccag ggcagggatc tctttaaaat agcgcagcgc acgcagttgc
     2341 gttaacgcgg ggtgtgacat ggtatatctc ctgtcgactt agccggacgc gctcagcggc
     2401 gccgccgggg tgttacccgc cgccagggtg gcggccgcgc cgccaccgga cacgtctttt
     2461 tcaccgctgc ttttcggttt gtacaggttc gggtaggtgt aggtcgggtt accacccgcc
     2521 ttgaactcct tccacgcgcg ttccacttcg tcgaacgcgt tgtagcccca caggaagtcc
     2581 tggaacaccg ggtcagcgaa gcggttcggg cagcccagtt ccggctgttt gtcgtacagg
     2641 taagagtcgc ggatttcctg cagttcaccg tccggcaggt ggtagatgtc ctgacactgg
     2701 tgagagccgc gcaccacctg agaagagcgc catttacgca ggttctccgt catctgcagc
     2761 agctgcggga tctggtcacg cagttcgatc ttggagaaca ggccagccag cacagcaccg
     2821 tcctccaccg cctgggacgc accagaggcc aggtacggca gggtggagtg ggaagcgtca
     2881 cccaggatga taaagttgcc ggttttggag tttacccacg ttttcagttc acgggagtcc
     2941 tgcagacgcc atttagacac gctggtgatc agtttgaaaa cggtggtcag gtcctggtcc
     3001 caacctttaa ccaggtccag cagctcctct tggctggcat cctgtttcag aacgcctttc
     3061 ggcagggtgt cagggcacag ggcaacaaca ttacagattt caccttcgtg caggaaatac
     3121 attacgatgt gcatggtcgg accccaccag aagttgatgt tcgggttggc gtaaaatttc
     3181 tccaggccag gaactttctt catatcttca actttgatca gtgcacggta tgccagatca
     3241 ccagtatcgt aagcgccaga agagtcacca gtcagcagag cacgggtctg gctacgtacg
     3301 ccatcatagc ctacgattac atcaccagag tactgtttgc cagttgcagt agtcacagtt
     3361 gctttttcaa aatcgatatc aacaacacgg ctattggtgt ggatttcaac acccagttct
     3421 tcggcacgat caaccagtac tttatgataa tcagcgcggt gaatatgcag atacatgcca
     3481 tcgtattttt ccagaacata aggcaccagg ttctgcgtag acagcagttc acccttatat
     3541 ttcttgacca ggatatcatg cggatgaatg gagactttat caactgcatc cagtacgcca
     3601 attgctttca gaatcttggt gctcggcggt ggaatttgaa taccagcgcc aacttcaccc
     3661 agttcagctg cttccagcag aatgacacgg tggcctgcca gacgcaggca aatagaagcc
     3721 gcgacgccac ccagacccgc acctacaacg acaaacgtca gttggattgg agctttttgg
     3781 aaaccataag ttttgcttgg tgcctgaact gccatggtac ctttctcctc tttaatgaat
     3841 tcggtcagtg cgtcctgctg atgtgctcag tatcttgtta tccgctcaca atgtcaattg
     3901 ttatccgctc acaattctcg aggtgaagac gaaagggcct cgtgatacgc ctatttttat
     3961 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4021 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4081 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4141 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4201 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4261 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4321 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4381 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4441 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4501 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4561 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4621 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4681 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4741 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4801 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4861 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4921 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4981 attggtaact gtcagaccaa gtttacgagc tcgcttggac tcctgttgat agatccagta
     5041 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     5101 attggtgaga atccaagcac tagtcatgac caaaatccct taacgtgagt tttcgttcca
     5161 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5221 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5281 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5341 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5401 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5461 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5521 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5581 acagcgtgag ctatgagaaa gcgccacgct tcccgaagg
//
