LOCUS       Exported                4877 bp ds-DNA     circular UNA 10-SEP-2025
DEFINITION  natural circular DNA
ACCESSION   urn.local...46-jn1vt7q
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             137..997
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="/Original_Translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAE 
                     DQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRI 
                     HYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLH 
                     NMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWM 
                     EADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAT 
                     MDERNRQIAEIGASLIKHW*"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1168..1756
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/snapgene_direction=RIGHT"
     misc_feature    complement(1894..1916)
                     /label=CRISPR guide 6
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=ACTCGCTGCGCTCGGTCGTTCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=ACTCGCTGCGCTCGGTCGTT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(1902..1924)
                     /label=CRISPR guide 5
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=GCTCACTGACTCGCTGCGCTCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=GCTCACTGACTCGCTGCGCT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    1906..1928
                     /label=CRISPR guide 1
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=AGG"
                     /note="/Recognition_pattern=GCGCAGCGAGTCAGTGAGCGAGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=GCGCAGCGAGTCAGTGAGCG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    1912..1934
                     /label=CRISPR guide 2
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=CGAGTCAGTGAGCGAGGAAGCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CGAGTCAGTGAGCGAGGAAG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    1942..1964
                     /label=CRISPR guide 3
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=TGG"
                     /note="/Recognition_pattern=AGCTTCGCACGATTGCACCTTGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=AGCTTCGCACGATTGCACCT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    1943..1965
                     /label=CRISPR guide 4
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=GCTTCGCACGATTGCACCTTGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=GCTTCGCACGATTGCACCTT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    1969..2038
                     /label=Dn29 attP
                     /note="/created_by=aliso"
                     /note="/modified_by=aliso"
     unsure          2003..2004
                     /label=core
                     /note="/created_by=aliso"
     gap             2043..2054
                     /estimated_length=12
     misc_feature    complement(2056..2078)
                     /label=CRISPR guide 19
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=TCGATCGAGGTTGCATTCGGCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=TCGATCGAGGTTGCATTCGG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(2059..2081)
                     /label=CRISPR guide 18
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=GGCTCGATCGAGGTTGCATTCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=GGCTCGATCGAGGTTGCATT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2061..2083
                     /label=CRISPR guide 1
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=GAATGCAACCTCGATCGAGCCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=GAATGCAACCTCGATCGAGC"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2062..2084
                     /label=CRISPR guide 2
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=AATGCAACCTCGATCGAGCCGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=AATGCAACCTCGATCGAGCC"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2068..2090
                     /label=CRISPR guide 3
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=ACCTCGATCGAGCCGGGCTCCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=ACCTCGATCGAGCCGGGCTC"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(2069..2091)
                     /label=CRISPR guide 17
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=AGG"
                     /note="/Recognition_pattern=ACCGGAGCCCGGCTCGATCGAGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=ACCGGAGCCCGGCTCGATCG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(2080..2102)
                     /label=CRISPR guide 16
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=CACTGACGGGCACCGGAGCCCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CACTGACGGGCACCGGAGCC"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2081..2103
                     /label=CRISPR guide 4
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=TGG"
                     /note="/Recognition_pattern=CGGGCTCCGGTGCCCGTCAGTGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CGGGCTCCGGTGCCCGTCAG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2082..2104
                     /label=CRISPR guide 5
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=GGGCTCCGGTGCCCGTCAGTGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=GGGCTCCGGTGCCCGTCAGT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(2087..2109)
                     /label=CRISPR guide 15
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=CTCTGCCCACTGACGGGCACCGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CTCTGCCCACTGACGGGCAC"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(2093..2115)
                     /label=CRISPR guide 14
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=TGTGCGCTCTGCCCACTGACGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=TGTGCGCTCTGCCCACTGAC"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(2094..2116)
                     /label=CRISPR guide 13
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=CGG"
                     /note="/Recognition_pattern=ATGTGCGCTCTGCCCACTGACGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=ATGTGCGCTCTGCCCACTGA"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2118..2140
                     /label=CRISPR guide 6
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=TGG"
                     /note="/Recognition_pattern=GCCCACAGTCCCCGAGAAGTTGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=GCCCACAGTCCCCGAGAAGT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2119..2141
                     /label=CRISPR guide 7
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=CCCACAGTCCCCGAGAAGTTGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CCCACAGTCCCCGAGAAGTT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(2119..2141)
                     /label=CRISPR guide 12
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=CCCAACTTCTCGGGGACTGTGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CCCAACTTCTCGGGGACTGT"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2120..2142
                     /label=CRISPR guide 8
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=CCACAGTCCCCGAGAAGTTGGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CCACAGTCCCCGAGAAGTTG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    complement(2120..2142)
                     /label=CRISPR guide 11
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=TGG"
                     /note="/Recognition_pattern=CCCCAACTTCTCGGGGACTGTGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CCCCAACTTCTCGGGGACTG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2121..2143
                     /label=CRISPR guide 9
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=CACAGTCCCCGAGAAGTTGGGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=CACAGTCCCCGAGAAGTTGG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     misc_feature    2122..2144
                     /label=CRISPR guide 10
                     /label=nonstandard type:  CRISPR
                     /note="/#_Off-target_Sites=0 (0 in CDS)"
                     /note="/Enzyme=SpCas9"
                     /note="/Protospacer_Adjacent_Motif_(PAM)=GGG"
                     /note="/Recognition_pattern=ACAGTCCCCGAGAAGTTGGGGGG(-6/-6)"
                     /note="/SpCas9=Cuts 3-4bp upstream of the PAM, displayed at
                     3bp"
                     /note="/Target_Sequence=ACAGTCCCCGAGAAGTTGGG"
                     /note="/Zhang_(2013)_Specificity_Score=100.00%"
     intron          2314..3251
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    3262..3271
                     /label=Kozak seq
     CDS             3268..3864
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="/Original_Translation=MTEYKPTVRLATRDDVPRAVRTLAAAFADY
                     PATRH 
                     TVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGP 
                     RMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAA 
                     ERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             3865..3921
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding 
                     separatepolypeptides."
                     /note="/Original_Translation=ATNFSLLKQAGDVEENPGP"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3928..4638
                     /codon_start=1
                     /gene="mCherry"
                     /product="red fluorescent protein mCherry"
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein"
                     /note="codon-optimized for expression in mammalian cells"
                     /note="/dnas_title=mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    4669..4876
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt ggctctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1561 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1801 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1861 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1921 gagcgaggaa gcggaagagc gagcttcgca cgattgcacc ttgggagccc taaactccca
     1981 cagataaaca gttaatggta atgaaataac attaactgtt tatctgtgtt taatgcctgc
     2041 cgnnnnnnnn nnnngccgcc gaatgcaacc tcgatcgagc cgggctccgg tgcccgtcag
     2101 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     2161 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     2221 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     2281 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     2341 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac tggctgcagt
     2401 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2461 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2521 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2581 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2641 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2701 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2761 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2821 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     2881 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     2941 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3001 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3061 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3121 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3181 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3241 ttccatttca ggtgtcgtga agccaccatg accgagtaca agcccacggt gcgcctcgcc
     3301 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     3361 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     3421 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     3481 gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     3541 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     3601 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggagtctcg
     3661 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     3721 gagcgcgccg gggtgcccgc cttcctggaa acctccgcgc cccgcaacct ccccttctac
     3781 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     3841 tgcatgaccc gcaagcccgg tgccgccacg aacttctctc tgttaaagca agcaggtgac
     3901 gtggaagaaa accccggtcc cggatccatg gtgagcaagg gcgaggagga taacatggcc
     3961 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     4021 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4081 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     4141 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     4201 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4261 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4321 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4381 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4441 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     4501 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     4561 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     4621 gacgagctgt acaagtaact cgagctagag ctcgctgatc agcctcgact gtgccttcta
     4681 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4741 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4801 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4861 gcaggcatgc tggggac
//
