LOCUS       Exported                2950 bp ds-DNA     circular UNA 10-SEP-2025
DEFINITION  natural circular DNA
ACCESSION   urn.local...2o-anuc0sj
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        7..247
                     /label=promoter
     unsure          256..275
                     /label=H1-g3 spacer
                     /note="/created_by=aliso"
     misc_RNA        276..351
                     /standard_name="gRNA scaffold"
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus
                     pyogenes CRISPR/Cas9 system"
     rep_origin      433..888
                     /label=origin
     promoter        1170..1274
                     /label=promoter
     CDS             1275..2135
                     /codon_start=1
                     /gene="bla"
                     /standard_name="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2306..2894
                     /direction=RIGHT
                     /label=origin
ORIGIN
        1 gctagcgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
       61 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
      121 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      181 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      241 aaaggacgaa acaccagtgt gtattctgct gttgtgtttt agagctagaa atagcaagtt
      301 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttga
      361 attctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
      421 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      481 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
      541 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      601 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
      661 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      721 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
      781 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
      841 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
      901 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      961 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     1021 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     1081 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1141 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1201 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1261 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1321 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1381 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1441 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1501 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1561 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1621 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1681 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1741 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1801 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1861 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1921 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1981 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2041 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2101 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2941 cttttgctca
//
