LOCUS       WT_FLAG-HaloTag-       11868 bp DNA     circular SYN 20-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 13, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..11868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             730..822
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    835..856
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        871..901
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    905..927
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     misc_feature    complement(962..985)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     protein_bind    1981..2014
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(2015..2239)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(2275..2874)
                     /codon_start=1
                     /label=PuroR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(2884..3394)
                     /label=hPGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    3395..3428
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(3531..3642)
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     polyA_signal    complement(3645..3766)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     polyA_signal    complement(3767..3888)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     polyA_signal    complement(3889..4010)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(4407..4472)
                     /codon_start=1
                     /label=3xFLAG
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(4473..5360)
                     /codon_start=1
                     /label=HaloTag(R)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             complement(5373..5393)
                     /codon_start=1
                     /label=TEV site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="EDLYFQS"
     CDS             complement(5658..9212)
                     /codon_start=1
                     /label=RTEL1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=97.2"
                     /note="/other=CDS"
                     /translation="QKVNGILESPTGTGKTLCLLCTTLAWREHLRDGISARKIAERAQG
                     ELFPDRALSSWGNAAAAAGDPIACYTDIPKIIYASRTHSQLTQVINELRNTSYRPKVCV
                     LGSREQLCIHPEVKKQESNHLQIHLCRKKVASRSCHFYNNVEEKSLEQELASPILDIED
                     LVKSGSKHRVCPYYLSRNLKQQADIIFMPYNYLLDAKSRRAHNIDLKGTVVIFDEAHNV
                     EKMCEESASFDLTPHDLASGLDVIDQVLEEQTKAAQQGEPHPEFSADSPSPGLNMELED
                     IAKLKMILLRLEGAIDAVELPGDDSGVTKPGSYIFELFAEAQITFQTKGCILDSLDQII
                     QHLAGRAGVFTNTAGLQKLADIIQIVFSVDPSEGSPGSPAGLGALQSYKVHIHPDAGHR
                     RTAQRSDAWSTTAARKRGKVLSYWCFSPGHSMHELVRQGVRSLILTSGTLAPVSSFALE
                     MQIPFPVCLENPHIIDKHQIWVGVVPRGPDGAQLSSAFDRRFSEECLSSLGKALGNIAR
                     VVPYGLLIFFPSYPVMEKSLEFWRARDLARKMEALKPLFVEPRSKGSFSETISAYYARV
                     AAPGSTGATFLAVCRGKASEGLDFSDTNGRGVIVTGLPYPPRMDPRVVLKMQFLDEMKG
                     QGGAGGQFLSGQEWYRQQASRAVNQAIGRVIRHRQDYGAVFLCDHRFAFADARAQLPSW
                     VRPHVRVYDNFGHVIRDVAQFFRVAERTMPAPAPRATAPSVRGEDAVSEAKSPGPFFST
                     RKAKSLDLHVPSLKQRSSGSPAAGDPESSLCVEYEQEPVPARQRPRGLLAALEHSEQRA
                     GSPGEEQAHSCSTLSLLSEKRPAEEPRGGRKKIRLVSHPEEPVAGAQTDRAKLFMVAVK
                     QELSQANFATFTQALQDYKGSDDFAALAACLGPLFAEDPKKHNLLQGFYQFVRPHHKQQ
                     FEEVCIQLTGRGCGYRPEHSIPRRQRAQPVLDPTGRTAPDPKLTVSTAAAQQLDPQEHL
                     NQGRPHLSPRPPPTGDPGSQPQWGSGVPRAGKQGQHAVSAYLADARRALGSAGCSQLLA
                     ALTAYKQDDDLDKVLAVLAALTTAKPEDFPLLHRFSMFVRPHHKQRFSQTCTDLTGRPY
                     PGMEPPGPQEERLAVPPVLTHRAPQPGPSRSEKTGKTQSKISSFLRQRPAGTVGAGGED
                     AGPSQSSGPPHGPAASEWG"
     CDS             10197..10354
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=90.8"
                     /note="/other=CDS"
                     /translation="IHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPN
                     SCAA*MA"
     misc_feature    complement(10360..10459)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=70.9"
                     /note="/other=misc_feature"
     CDS             complement(10561..10596)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     promoter        10691..10795
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             10796..11656
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      join(11827..11868,1..547)
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      961 caagcttgca tgcctgcagg tcgaccagga acccgacaga acaccaagac tctgctgggc
     1021 tctgacaagc agcagggcct cctcctactt catgtgcaaa gctgcactgg gggccagccc
     1081 tgacctactg cacagctgct acaacagcac ctgcgaggag ctcaggacgc ccgcccaagg
     1141 tcagcacaca gatgcaaagc tgcttcctca caagtaatgg gcattggagg cagcccaggc
     1201 cgcaccccac ccctgggcac cagcgctctg cacacttcgg gggagcgtct agaaagacca
     1261 ccaccctcca ccacagcaca gcatggggct gtggactcag caccatgcca agctggttct
     1321 cttgaggacc ccgagacagg tgtaaactcg tctgacccac cggtaggagg tgttccgaag
     1381 ctcgttgatg acctgtgtga gttgcgagtg ggtcctggag gcgtaaataa tctttgggat
     1441 gtccgtgtag caagctgacc agacacaagc aagcacagaa gtcacggttc tcagacaacg
     1501 ttctcacgta tcaaaggcag gtggcattcg gctaactcgg ccctgcagac agccaagaat
     1561 ctgacacccc agatactggt ttatcaaaaa tgggtgaagt gggcatctgc cgtgccctaa
     1621 ttcagcaaaa aagcccaacc ctaggtttac acccacgatg gcgagaaatt atacagcagc
     1681 taaaaaattc tccttgagtt ctgcttgaga gacggcagga gtccccatgg gcccacccca
     1741 ccctcagcgc catcccttgc caaccatccc cacaggaggc caggagaggc ctgggaacta
     1801 gggtcaccta tggggtctcc agcagcagca gcagcgttgc cccaggatga caaggcccga
     1861 tccgggaaaa gctctccttg cgccctctcg gcaatcttgc gggcagagat gccgtctcgg
     1921 aggtgttctc gccaggccag cgtggtgcac agcaggcaca gcgtcttccc tgtacctagg
     1981 ataacttcgt atagcataca ttatacgaag ttatccatag agcccaccgc atccccagca
     2041 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     2101 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     2161 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     2221 tggcaactag aaggcacagc ctgcagggtt taaaccaatt ggtctagact cgagtcaggc
     2281 accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt
     2341 gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa
     2401 ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag
     2461 acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt
     2521 gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct
     2581 caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg
     2641 cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag
     2701 cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg
     2761 atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc
     2821 cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggtgaa
     2881 ttgctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc
     2941 cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc
     3001 acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca
     3061 gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc
     3121 gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa
     3181 ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag
     3241 ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg
     3301 gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc
     3361 agggctgcct tggaaaaggc gcaaccccaa ccccataact tcgtatagca tacattatac
     3421 gaagttatga tatcgcggcc gcatgcctgc tattctcttc ccaatcctcc cccttgctgt
     3481 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
     3541 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
     3601 gggaggggca aacaacagat ggctggcaac tagaaggcac agtctaagat acattgatga
     3661 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3721 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagtttaag atacattgat
     3781 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3841 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttta agatacattg
     3901 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     3961 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt ggtacctggg
     4021 cactgccagc agctttatta gagagccctg tcccctgatt aaaagtggac acgtgcccac
     4081 gggggtctcc tccttcccgg tgaggggctc agctggggtg cccctgtggc accactcaca
     4141 ggccaggagc cagggaagtt ctggggaagc actttcccag aaacccaccc cagaccccaa
     4201 ggtagctcag ggcttctcag aggcaggccc agatccggtc ccaccgcagg gaccaaccat
     4261 gggccccgcc tgcctgaggc ctgcagcggg ccaagccggc tggcatgggc tggcctattc
     4321 tgttgggtgg gttcttggcc caccagagct ggacaggcag gtgatcaagc cacgttgggt
     4381 gtgctggggg cctccgtggg cactcacttg tcatcgtcat ccttgtaatc gatgtcatga
     4441 tctttataat caccgtcatg gtctttgtag tcgccggaaa tctcgagcgt tgacagccag
     4501 cgcgcgatct cgctgccgat caggtccggg ttgtcttctt gcagcagatt cagacccggg
     4561 ccgatgtcca cagccttgca gttaggcagg cttttggcca ggcgagcggc ttcggccggt
     4621 gggatcagaa cgcctggggt gccccagaac agcagcttcg ggacagggga ctggtgcagc
     4681 cagtccatgt attcttcgac cagcgcgacg atgttcgctg gctcaccggc gattggcagc
     4741 tcgtttggga agcgccacag tggctcgcgg tcaacaggat tcaggaacgg ctcgcggtaa
     4801 tggtccatct cgacttcagt cagcgggcgg acgacaccca tcggcagcgt accctcgata
     4861 aaaacgttct gatcgatgat cagcttgcgg ccgacgtcgg tggtgcggaa ggcctggaag
     4921 gtctcgcggg caaattctgg ccattcgtcc caggtcggga tagggcggat gaactccata
     4981 aatgcaatac ctttgacgcg ctctggattg cgcttggccc agtggaaacc cagagcggag
     5041 ccccagtcgt gaatgaccag gacgacctct tccagaccca gggcttcgat gaaggcatcc
     5101 atgaagcgga cgtggtcgtc gaagaaataa cccaggtctg gtttgtcgga tttgcccata
     5161 ccgatcaggt ctggagcaat gcagcgatgg gtcggtgcaa catgcgggat gatgttgcgc
     5221 cacacgtagg aggaggtcgg gttaccgtgc aggaacagca caggggtgcc atcgcgcgga
     5281 ccaacatcga cgtagtgcat gcgctcgccc aggacttcca cataatgggg gtcgaatgga
     5341 aagccagtac cgatttctgc gatcgcgtta tcgctctgaa agtacagatc ctcagtggtt
     5401 ggctcggtac cctggggctc tggccagaag acctgcatga cgctctgctt cctggaggcg
     5461 gtgtggcagg ctgggcacat cctagaggcc tgaaggtgcc gttgccagca ggcttggcag
     5521 cgctggaagt cacaggcagg gcactggaag ggcaccacgt cctctgcccc acagccctgg
     5581 cacacacagc ctgctgagag gggcccgccc ggagctcccg tggcctgctg cccagcgatg
     5641 tctctcccat gaggctcacc ccactcagat gctgcaggcc cgtggggagg tcctgaggac
     5701 tggctgggac ctgcatcctc accgcccgcc cccacagtcc ctgctggcct ctgtctaagg
     5761 aaggacgaga tcttgctctg ggtcttcccg gtcttctcgg accgtgaggg gcctggttgg
     5821 ggagccctgt gggtaagcac aggaggcacg gcaagcctct cctcctgggg tcccggtggc
     5881 tccatgcccg ggtagggccg gccggtcagg tctgtgcacg tctgtgagaa gcgctgcttg
     5941 tggtgtggac gcacaaacat gctgaacctg tgcagcaggg ggaagtcctc tggctttgca
     6001 gtggtcaggg cggccaacac agccagcacc ttgtcgaggt cgtcgtcttg cttataggct
     6061 gtcagcgctg ccaagagttg gctacagccc gcggacccca gggccctgcg ggcatcagcc
     6121 aggtaggcgc tcacggcgtg ctggccctgc ttccctgctc tgggcactcc agacccccac
     6181 tgtggttggc tgccagggtc tcctgttggg ggtggcctgg gcgacaggtg gggcctgccc
     6241 tggttcaggt gctcttgggg gtccagctgc tgggctgcag ccgtggacac ggtcagcttg
     6301 ggatccggcg ccgttcttcc agtggggtcc aggaccggct gtgcccgctg ccttcgggga
     6361 atgctgtgct caggccgata gccacagcct cgtcctgtca gctggataca gacctcctca
     6421 aactgctgct tatggtgggg ccgcacaaac tggtagaagc cttggagcag gttgtgcttc
     6481 ttggggtcct cagcaaagag ggggccgaga caggcggcca gggcggcgaa gtcatcggaa
     6541 cccttgtagt cctgcagggc ctgggtgaag gtggcaaagt tggcttggct caactcctgc
     6601 ttcacggcca ccatgaagag cttggccctg tccgtctgtg caccagccac gggctcctcc
     6661 gggtggctga ccagccggat cttcttcctc cctcctcgcg gttcttctgc cggcctcttc
     6721 tcagacagga gggacagggt ggagcagctg tgggcctgct cctcgccagg gctccccgcc
     6781 cgctgttcgc tgtgctccag ggcggccagc agccccctgg gcctctgccg ggcaggaact
     6841 ggctcctgct catactccac acacaggcta ctctcggggt ccccggcagc tggtgaccct
     6901 gaggacctct gcttcaggct ggggacatgc aggtccagac tcttagcttt cctggtggag
     6961 aagaaggggc caggcgactt ggcctcgctg acagcatctt ctccacgcac actgggtgct
     7021 gtagcccggg gggccggcgc tggcatagtt cgctcggcaa cacggaagaa ctgggccacg
     7081 tctcggatga catggccaaa gttgtcatac accctgacgt ggggacgcac ccaggagggc
     7141 agttgggctc ttgcgtcggc aaaggcgaac ctgtggtcac agaggaagac agctccgtag
     7201 tcctggcggt gccggatcac tcgcccgatg gcctggttca cagccctgga cgcctgctgc
     7261 cggtaccact cctgcccaga gaggaactgg cccccagccc caccctggcc cttcatctca
     7321 tccaggaact gcatcttgag gacaacccgg gggtccatgc gtggggggta cgggaggccc
     7381 gtgacaatca caccacggcc attcgtgtct gagaagtcca gcccctcgct ggccttgccc
     7441 cggcagaccg ccaggaaggt ggcgccggtg gacccagggg cggcaaccct tgcatagtaa
     7501 gcactgatgg tctcggagaa gctgcctttg ctcctgggct ccacaaacag cggcttcagc
     7561 gcctccatct tcctggccaa gtcgcgggcc cgccagaact ccaggctctt ctccatgaca
     7621 ggataggaag ggaagaagat caggagccca tagggcacca cgcgggcgat gttgcccaga
     7681 gccttcccca gggaggataa gcactcctcg gaaaaccgtc tgtcaaacgc ggagctcaac
     7741 tgggctccat cggggcctct ggggacgacc cccacccaga tctggtgctt gtcgatgatg
     7801 tgtgggttct ccaggcagac tgggaaaggg atctgcatct ccagagcaaa ggaggacacc
     7861 ggggccagcg tgccgctggt aaggatgagg gagcggacgc cctggcggac cagctcgtgc
     7921 atgctgtggc cgggactgaa gcaccagtag ctcagcacct tccctcgctt tctggctgca
     7981 gtggtgctcc aggcatcaga ccgctgagcc gtcctccggt gaccagcatc aggatggatg
     8041 tgcaccttat aggactgtaa ggcccccagc cctgctgggg aaccagggct gccctcggag
     8101 gggtccacac tgaacacaat ctggataatg tccgccagct tctgcagtcc ggccgtgttg
     8161 gtgaacactc cagcacgtcc tgccaggtgc tggatgatct ggtccagcga gtccaggatg
     8221 cagcccttgg tctgaaacgt gatctgggct tcagcaaaca gctcaaagat gtagctccct
     8281 ggcttggtga caccgctgtc gtctccaggc agctcaacag catcgatggc cccctccagg
     8341 cgcagcagga tcatcttcag ctttgcaatg tcttccagct ccatgttcag ccctgggctg
     8401 ggggagtccg cgctgaactc cgggtggggc tcaccctgct gcgctgcctt ggtctgctcc
     8461 tccagcacct ggtctatgac gtccagtcct gaagccaggt catggggagt caggtcaaag
     8521 gatgccgatt cttcacacat cttctccacg ttgtgagctt cgtcaaagat cacgactgtc
     8581 cccttcaggt caatgttgtg tgctctgcgg ctcttggcat ccaacaagta attgtacggc
     8641 atgaatatga tgtcggcttg ctgcttcagg ttccgggaca ggtagtaagg gcacaccctg
     8701 tgcttgcttc cgctcttgac caagtcctca atgtccagga tggggctggc cagctcctgc
     8761 tccaggcttt tttcttctac gttgttgtag aaatgacagg agcgacttgc caccttctta
     8821 cggcacaagt ggatctgtag atggttactc tcttgtttct tcacctcagg atggatgcac
     8881 agctgctccc gggagcccag cacacacacc ttaggccggt aggaggtgtt ccgaagctcg
     8941 ttgatgacct gtgtgagttg cgagtgggtc ctggaggcgt aaataatctt tgggatgtcc
     9001 gtgtagcaag cgataggatc gccggcagcg gcggcggcgt tgccccaact tgacagagct
     9061 ctatcaggga acagctcgcc ctgagccctc tctgcaattt ttctggcgct gattccgtct
     9121 ctcaggtgct ccctccaagc cagggttgtg cacagcaggc acagtgtttt gccagttcct
     9181 gtgggtgact ccaggatgcc attcaccttc tgaacggagt tcggagcaag ggttacgggg
     9241 aagccaggaa gaggcgaggc cacacaagct gccctgagac aagcttcggc agccagcggg
     9301 cagctctttg cagatgcaga ggccactgcc aggtggcgag agccctgtgg gaccgcggag
     9361 aagtccacag gcaagtccca ctcttgccct aaagggcgtg aagcaactca gccgccatta
     9421 gcaaacgatt tgcagaacgt tacccaaaac acgacactat ttttcagctt aaatagtaag
     9481 atgactaatt aggtgtcaag tctcaaaata ggaaactgtt ctgcgtttct ggatccaaac
     9541 atttatgctg tcctatctga cgtcctacat acaagctctc agacgggaac ctgagcagga
     9601 aactcgggga ggggaagggt gtgagccggg tacaagaggg cacctcaagg aacccaggag
     9661 aaggcaacct gcaccactgc cacctccctg cacccccgag gttcaaagac cttccaagtg
     9721 aggctggcca ccggctgccc ctctaatgga aatgcttcct cctgacccgt gaatatcagc
     9781 tgctcctcag agaggccccc aaggcactcc taagtctgtc accctcaagg actgtaggga
     9841 aaagagagat cagactgtta ctgtgtctat gtagaaaggg aagacataac acactccatt
     9901 ttgacctgta ccctgaacaa ttgctttgcc ctaagatgct gttaatctgt aactttgccc
     9961 cagccttgag ctcacagaaa catgtgttgt atggaatcaa ggtttaaggg atctagagct
    10021 gtgcagaacg tgccttgtta acaatatgtt tacaggcagt atgcttggta aaagtcatcg
    10081 ccattctcca gtctcgataa accaggggca cgatgcactg cggaaagccg cagggacctc
    10141 tgccctggaa agccgggtat tgcccaaggt ttctctccat gtgatagtga gctcgaattc
    10201 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
    10261 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
    10321 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
    10381 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
    10441 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
    10501 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
    10561 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
    10621 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
    10681 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10741 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    10801 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10861 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10921 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10981 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    11041 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    11101 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    11161 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    11221 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    11281 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    11341 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    11401 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    11461 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    11521 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    11581 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    11641 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    11701 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    11761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    11821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaac
//
