LOCUS       Exported               10586 bp DNA     circular SYN 30-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        584..688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             689..1549
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1720..2308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3624..3652)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4161..4209
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4294..5007)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        complement(5032..5141)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     enhancer        5327..5706
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5707..5910
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          6046..6178
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        6223..6241
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7807..7860
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9058..9123
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    9446..10034
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9917..9928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10176..10310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  10339..10504
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggttccg catgctatgc atccttgtac agctcgtcca tgccgagagt
     4321 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
     4381 tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
     4441 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
     4501 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
     4561 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
     4621 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
     4681 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
     4741 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
     4801 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
     4861 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
     4921 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
     4981 ggtgaacagc tcctcgccct tgctcaccgg gtgatcaagt cttcgtcgag tgattgtaaa
     5041 taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga taataattct
     5101 tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatactc cggaatatta
     5161 ataggatccc ggtccgaagc gcgcggaatt caatattggc cattagccat attattcatt
     5221 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
     5281 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttggca ttgattattg
     5341 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5401 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5461 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5521 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     5581 ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     5641 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     5701 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
     5761 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     5821 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt
     5881 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cactagaagc
     5941 tttattgcgg tagtttatca cagttaaatt gctaacgcag tcagtgcttc tgacacaaca
     6001 gtctcgaact taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt
     6061 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
     6121 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
     6181 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag
     6241 gctagcctcg aggcggccgc caccgcggcc gcggcctctg aggccaccat gacccccgga
     6301 gccctgctga tgctgctggg cgctctgggc gcccctctgg cccctggcgt gcggggctct
     6361 gaggccgagg gcagactgcg agaaaaactg ttcagcggct acgacagcag cgtgcggcct
     6421 gccagagaag tgggcgacag agtgcgggtg tccgtgggac tgatcctggc tcagctgatc
     6481 agcctgaatg agaaggatga ggaaatgtcc accaaggtgt acctggatct ggaatggacc
     6541 gactaccggc tgtcttggga ccctgctgag cacgatggca tcgactccct gagaatcact
     6601 gccgagagcg tttggctgcc cgatgtggtg ctcctcaaca acaacgacgg caacttcgac
     6661 gtggccctgg acatcagcgt ggttgtgtct tccgatggat ctgtgcggtg gcagcctcca
     6721 ggcatctaca gaagctcctg ctctatccag gtgacctact tcccattcga ctggcaaaat
     6781 tgcaccatgg tgttcagcag ctacagctat gattctagcg aggtgagcct gcagaccggc
     6841 ctgggacctg acggacaggg ccaccaggag atccacatcc acgagggcac cttcatcgag
     6901 aacggccagt gggaaatcat ccacaagccc agcagactta tccagccccc tggcgatcct
     6961 agaggcggaa gagaaggcca aagacaggag gtgatcttct acctgatcat cagacgcaaa
     7021 ccactgttct acctagtgaa cgtgatcgcc ccctgcatcc tgatcacact gctggccatc
     7081 tttgtgttct acctgcctcc agacgccggc gagaagatgg gcctgagcat cttcgccctg
     7141 ctgaccctga cagtgtttct gctgctgctg gccgacaagg ttcctgagac aagcctgagc
     7201 gtgcccatca taatcaagta cctgatgttc accatggtcc tggtgacctt tagcgtgatc
     7261 ctatctgtgg tcgtgctgaa cctgcaccac agatcccctc acacccacca gatgcccctg
     7321 tgggtgagac agatctttat ccataagctg cctctgtacc tgcggctgaa gagacctaag
     7381 cctgaacggg acctgatgcc cgagcctcct cattgtagca gccctggttc tggctgggga
     7441 agaggcacag atgagtactt cattagaaag cccccgagcg acttcctgtt ccctaaaccc
     7501 aacaggttcc agcctgaact gagcgcccct gacctgcgga gattcattga cggccctaac
     7561 agagccgtgg cacttctgcc cgagctgaga gaagtggtgt cgtctatcag ctatatcgcc
     7621 agacagctgc aagagcagga ggaccacgac gcccttaagg aagattggca gttcgtggcc
     7681 atggtcgtgg accggctgtt tctgtggacc ttcattatct ttaccagcgt gggcacactg
     7741 gtcatcttcc tggacgctac ataccacctg cctccacctg atcccttccc ctctagaggc
     7801 tccggcgagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca
     7861 gtcgacatgg ctagagcccc tctgggcgtt ctgctgctgc tgggcctcct gggcaggggc
     7921 gtgggaaaga acgaggagct ccggctgtac caccacctgt tcaacaatta cgaccccggc
     7981 agcagacctg tgcgggaacc cgaagatacc gttaccatca gcctgaaggt caccctgacc
     8041 aacctgatta gcctgaacga gaaagaggaa acactgacaa catctgtgtg gatcggcatc
     8101 gattggcagg actaccggct gaattattct aaggacgact tcggaggcat cgagacgctg
     8161 agggtgccta gcgagctggt gtggctgccc gagatcgtgc ttgaaaacaa catcgacggc
     8221 caatttggag ttgcttacga cgccaatgtg ctggtgtacg agggcggctc tgtgacctgg
     8281 cttcctcctg ccatctacag aagcgtgtgc gccgtcgagg tgacctactt ccccttcgac
     8341 tggcagaact gtagcctgat ctttagatcc cagacataca acgccgaaga ggtggagttc
     8401 accttcgccg tggacaacga cggcaagacc atcaacaaga tcgacatcga caccgaggcc
     8461 tacaccgaga acggcgagtg ggctatcgat ttctgccctg gagtgatccg gagacatcac
     8521 ggcggcgcta cagatggccc cggagaaacc gacgtgatct acagcctgat catcagaaga
     8581 aagcccctgt tctacgtgat caacatcatc gtaccatgtg tgctgatcag cggactggtg
     8641 ctgctggcct attttctgcc cgcccaggcc ggcggacaga aatgcaccgt gtccatcaat
     8701 gtgctgctcg cccaaaccgt gttcctgttc ctcatcgccc agaagatccc tgagacaagc
     8761 ctgagcgtgc ctctgctggg cagattctta atcttcgtga tggtggtggc caccctgatc
     8821 gtgatgaact gcgtgatcgt gctgaacgtg tcccagagaa cccctaccac acacgccatg
     8881 agccctcggc tgcggcacgt gctgctggaa ctgctgccaa gactgctggg cagccctcca
     8941 cctcctgagg ctcctagagc cgctagccct ccacggagag ccagcagcgt gggcctgctg
     9001 ctgagagctg aagaactcat cctgaaaaag cctcgatctg aactggtgtt cgagggcgac
     9061 tacaaggatc atgacggcga ctacaaggac cacgacatcg actacaagga cgacgatgac
     9121 aagcaaagac acagacaggg gacatggacc gccgcatttt gccagagcct gggcgccgcc
     9181 gcccctgaag tgcgctgctg cgtggatgcc gtgaacttcg tggccgagag cacaagagat
     9241 caggaggcca ccggcgaaga ggtcagcgac tgggtccgga tgggcaacgc cctggataac
     9301 atttgcttct gggcggccct ggtgctgttc agcgtgggct ctagcctgat cttcctgggg
     9361 gcttacttca atagagtgcc cgacctgcct tacgccccct gtatccagcc ttgaggcctg
     9421 tcaggccggt accggtacca agcttaatca acctctggat tacaaaattt gtgaaagatt
     9481 gactggtatt cttaactatg ttgctccttt tacgctatgt gaatacgctg ctttaatgcc
     9541 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     9601 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     9661 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     9721 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     9781 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     9841 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     9901 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     9961 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
    10021 ggccgcctcc ccgcctgcag tctgacaagc ttgtcgagaa gtactagagg atcataatca
    10081 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    10141 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    10201 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10261 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc
    10321 ttgagcctag gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt
    10381 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
    10441 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
    10501 cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt
    10561 gacaaaccgt catcttcggc tacttt
//
