LOCUS       pcDNA3.1/Puro-CAG-JEDI- 7388 bp ds-DNA     circular     15-SEP-2025
DEFINITION  .
ACCESSION   urn.local...e-6d8skt5
VERSION     urn.local...e-6d8skt5
COMMENT     VADER2 P1G5 K167W C219L Q398P Imported using the Genbank importer.
            File name: 0057 pc3-puro-CAG-ccdB-camR simplified.gb Concatenation
            of 2 sequences.
FEATURES             Location/Qualifiers
     terminator      86..205
                     /label="SV40 PA terminator"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      complement(640..1259)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(1414..2274)
                     /label="Ampicillin CDS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        complement(2316..2344)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2430..2763
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        2770..4135
                     /label="Actin promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4253..5536
                     /label="JEDI-2Psub"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     terminator      5657..5884
                     /label="bGH PA terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      5947..6253
                     /label="f1 origin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(6373..6588)
                     /label="SV40 enhancer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        6385..6653
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
       61 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
      121 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      181 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
      241 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      301 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      361 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      421 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      841 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1081 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1261 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1321 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2401 acctgacgtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2461 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2521 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2581 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2641 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2701 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2761 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     2821 ctccccatct ccccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     2881 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     2941 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     3001 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     3061 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     3121 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     3181 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     3241 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     3301 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     3361 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     3421 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     3481 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     3541 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     3601 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     3661 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     3721 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     3781 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     3841 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     3901 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     3961 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     4021 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     4081 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     4141 cctcgaggaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     4201 caatgccctg gctcacaaat accactgaga tctgcatgag ctagccgcca ccatggagac
     4261 gactgtgagg tatgaacagg ggtcagagct cactaaaact tcgagctctc caacagcaga
     4321 tgagcccacg ataaagattg atgatggtcg tgatgagggt aatgaacaag acagctgttc
     4381 caataccatt aggagaaaaa tttccccgtt tgtgatgtca tttggattca gagtatttgg
     4441 agttgtgctt atcattgtag acatcatagt ggtgattgtg gatctggcca tcagtgagaa
     4501 gaaaagaggc attagagaga ttcttgaagg tgtttccctg gctatagcac tcttcttcct
     4561 tgttgatgtt ctcatgagag tgtttgttga aggcttcaag aactatttcc ggtccaaact
     4621 ggttactttg gatgcagtca tagtagtggg cactctgcta attaatatga cctactcctt
     4681 ctctgacctt gctgccttta acagcgagaa cgtgtatatt accgcggata aacagaaaaa
     4741 cggcattaaa gcgaacttta ccgtgcgcca taacgtggaa gatggcagcg tgcagctggc
     4801 ggatcattat cagcagaaca ccccgattgg cgatggcccg gtgctgctgc cggataacca
     4861 ttatctgagc caccagaccg tgctgagcaa agatccgaac gaaaaacgcg atcacatggt
     4921 gctgctggaa tttgtgaccg cagcgggcat tacacacggc atggatgaac tgtatggcgg
     4981 caccggcggc agcgcgagcc agggcgaaga actgtttacc ggcgtggtgc cgattctggt
     5041 ggaactggat ggcgatgtga acggccataa atttagcgtg cgcggcgaag gcgaaggcga
     5101 tgcgaccatt ggcaaactga ccctgaaatt tatttgcacc accggcaaac taccggtgcc
     5161 gtggccgacc ctggtgacca ccttaaccta tggcgtgcag tgctttagcc gctatccgga
     5221 tcatatgaaa cgccatgatt tttttaaaag cgcgatgccg gaaggctatg tgcaggaacg
     5281 caccattagc tttaaagatg atggcaaata taaaacccgc gcggtggtga aatttgaagg
     5341 cgataccctg gtgaaccgca ttgaactgaa aggcaccgat tttaaagaag atggcaacat
     5401 tctggggcat aaactggaat atgattggac agatcagatg ccgcacatgg ttactcttct
     5461 tcgagttctg aaaattgtta tcttaataag aatatttcgc ctggcttcac AGAAGAAACA
     5521 ACTTGAAGTG GTAACCtaat gaaagcttgg taccgagctc ggatccacta gtccagtgtg
     5581 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     5641 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5701 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5761 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5821 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5881 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5941 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6001 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6061 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6121 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6181 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6241 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6301 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     6361 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6421 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6481 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6541 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6601 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6661 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg catgaccgag
     6721 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6781 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6841 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6901 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6961 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7021 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7081 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7141 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7201 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7261 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg attcgaaatg
     7321 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     7381 gaaaggtt
//