LOCUS       pAAV-EF1a-DIO-JEDI-2Psu 6901 bp ds-DNA     circular     16-SEP-2025
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct .
COMMENT     Imported using the Genbank importer. File name: 2299
            pAAV-EF1a-DIO-JEDI-2P-WPRE.gb Cre-activated AAV expression of
            JEDI-2P.
FEATURES             Location/Qualifiers
     repeat_region   2..131
                     /label="AAV2 ITR "
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        232..1410
                     /label="EF-1-alpha promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1587..2870
                     /label="JEDI-2Psub"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3029..3617
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    3649..4125
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   4165..4305
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     rep_origin      4380..4835
                     /label="f1 ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        5117..5221
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5222..6082
                     /label="AmpR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     rep_origin      6253..6841
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt ttaaacagag
      181 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
      241 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      301 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      361 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      421 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      481 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      541 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      601 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      661 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      721 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      781 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      841 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
      901 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
      961 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1021 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1081 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1141 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1201 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1261 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1321 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1381 agtttttttc ttccatttca ggtgtcgtga ggtaccggat cctctagagt cgactccgga
     1441 ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg attgtagctg
     1501 ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac gaagttatgg
     1561 cgcgccctat tacttgtaca tcattaggtt accacttcaa gttgtttctt ctgtgaagcc
     1621 aggcgaaata ttcttattaa gataacaatt ttcagaactc gaagaagagt aaccatgtgc
     1681 ggcatctgat ctgtccaatc atattccagt ttatgcccca gaatgttgcc atcttcttta
     1741 aaatcggtgc ctttcagttc aatgcggttc accagggtat cgccttcaaa tttcaccacc
     1801 gcgcgggttt tatatttgcc atcatcttta aagctaatgg tgcgttcctg cacatagcct
     1861 tccggcatcg cgcttttaaa aaaatcatgg cgtttcatat gatccggata gcggctaaag
     1921 cactgcacgc cataggttaa ggtggtcacc agggtcggcc acggcaccgg tagtttgccg
     1981 gtggtgcaaa taaatttcag ggtcagtttg ccaatggtcg catcgccttc gccttcgccg
     2041 cgcacgctaa atttatggcc gttcacatcg ccatccagtt ccaccagaat cggcaccacg
     2101 ccggtaaaca gttcttcgcc ctggctcgcg ctgccgccgg tgccgccata cagttcatcc
     2161 atgccgtgtg taatgcccgc tgcggtcaca aattccagca gcaccatgtg atcgcgtttt
     2221 tcgttcggat ctttgctcag cacggtctgg tggctcagat aatggttatc cggcagcagc
     2281 accgggccat cgccaatcgg ggtgttctgc tgataatgat ccgccagctg cacgctgcca
     2341 tcttccacgt tatggcgcac ggtaaagttc gctttaatgc cgtttttctg tttatccgcg
     2401 gtaatataca cgttctcgct gttaaaggca gcaaggtcag agaaggagta ggtcatatta
     2461 attagcagag tgcccactac tatgactgca tccaaagtaa ccagtttgga ccggaaatag
     2521 ttcttgaagc cttcaacaaa cactctcatg agaacatcaa caaggaagaa gagtgctata
     2581 gccagggaaa caccttcaag aatctctcta atgcctcttt tcttctcact gatggccaga
     2641 tccacaatca ccactatgat gtctacaatg ataagcacaa ctccaaatac tctgaatcca
     2701 aatgacatca caaacgggga aatttttctc ctaatggtat tggaacagct gtcttgttca
     2761 ttaccctcat cacgaccatc atcaatcttt atcgtgggct catctgctgt tggagagctc
     2821 gaagttttag tgagctctga cccctgttca tacctcacag tcgtctccat ggtggcggta
     2881 gctagcataa cttcgtataa agtatcctat acgaagttat ttgccttaac ccagaaatta
     2941 tcactgttat tctttagaat ggtgcaaaga ataacttcgt ataatgtatg ctatacgaag
     3001 ttatgaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     3061 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     3121 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3181 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3241 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3301 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3361 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3421 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     3481 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3541 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3601 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     3661 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     3721 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     3781 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     3841 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     3901 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     3961 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     4021 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     4081 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     4141 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4201 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4261 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4321 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4381 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4441 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4501 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4561 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4621 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4681 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     4741 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4801 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4861 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4921 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4981 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5041 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5101 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5161 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5221 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5281 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5341 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5401 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5461 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5521 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5581 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5641 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5701 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5761 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5821 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5881 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5941 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6001 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6061 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6121 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6181 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6241 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6301 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6361 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6781 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6841 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6901 g
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