LOCUS       EK0574_CAG-ADAR1-p150   9970 bp ds-DNA     circular     26-JUL-2025
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     CDS             complement(170..1030)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             complement(170..1030)
                     /label="Translation 1030-170"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer          complement(295..313)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1003..1028)
                     /label="oLSM0054"
                     /note="sequence: gagtattcaacatttccgtgtcgccc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1003..1034
                     /label="oLSM0053"
                     /note="sequence: gggcgacacggaaatgttgaatactcatactc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(1031..1129)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1125..1141
                     /label="AmpR_seq_F"
                     /note="sequence: ggggttccgcgcacatt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1242..1262
                     /label="FlpInF1"
                     /note="sequence: agtatctgctccctgcttgtg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1306..1323
                     /label="FlpInF"
                     /note="sequence: aacaaggcaaggcttgac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1306..1324
                     /label="EF1a_F"
                     /note="sequence: aacaaggcaaggcttgacc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1313..1345
                     /label="oLSM039"
                     /note="sequence: caaggcttgaccgacaattgcatgaagaatctg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1317..1336
                     /label="FlpInF2"
                     /note="sequence: gcttgaccgacaattgcatg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1319..1359
                     /label="CCp003-EK300-triggerinsert-revcomp-fwdprimer-MunI"
                     /note="sequence: ttgaccgacaattgcatgaagaatctgcttagggttaggcg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1321..1343)
                     /label="EKo152.4"
                     /note="sequence: gattcttcatgcaattgtcggtc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1336..1359
                     /label="pNSK165"
                     /note="sequence: gaagaatctgcttagggttaggcg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1377..1393
                     /label="EKo279.1"
                     /note="sequence: cgggccagatatacgcg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     enhancer        1396..1775
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1472..1494
                     /label="EK_CMVFa"
                     /note="sequence: cgttacataacttacggtaaatg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1548..1567
                     /label="EK_CMVFb"
                     /note="sequence: tatgttcccatagtaacgcc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1624..1644
                     /label="EK_CMVFc"
                     /note="sequence: tggcagtacatcaagtgtatc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1700..1718
                     /label="EK_CMVFd"
                     /note="sequence: ttatgcccagtacatgacc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1731..1752
                     /label="YX021-seq-2"
                     /note="sequence: cctacttggcagtacatctacg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        1778..2053
                     /label="chicken β-actin promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     intron          2054..3062
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     primer          3010..3027
                     /label="pCAG-F"
                     /note="sequence: gcggctctagagcctctg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3021..3044
                     /label="oLSM0026"
                     /note="sequence: gcctctgctaaccatgttcatgcc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3119..3152
                     /label="EKo538.1"
                     /note="sequence: ggaccgggtttaaacttaagcttcgccaccatgG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3122..3125
                     /label="overhang"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          3122..3136
                     /label="EKo283.1"
                     /note="sequence: ccgggtttaaactta"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3123..3162
                     /label="EKo574.1"
                     /note="sequence: cgggtttaaacttaagcttcgccaccatggggtcccgggc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(3126..3140)
                     /label="EKo283.2"
                     /note="sequence: agcttaagtttaaac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          3126..3147
                     /label="EWo100.1"
                     /note="sequence: gtttaaacttaagcttcgccac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(3126..3151)
                     /label="pNSK174"
                     /note="sequence: CATggtggcgaagcttaagtttaaac"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3134..3152
                     /label="EKo237.1"
                     /note="sequence: ttaagcttcgccaccatgg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3136..3151
                     /label="EKo271.3"
                     /note="sequence: aagcttcgccaccatg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3137..3152
                     /label="EKo309.1"
                     /note="sequence: agcttcgccaccatgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3141..3148
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             3149..3238
                     /label="Translation 3149-3238"
                     /translation="MGSRADYKDHDGDYKDHDIDYKDDDDKGRA"
     CDS             3164..3229
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3203..3226)
                     /label="pNSK154"
                     /note="sequence: gtcatcgtcatccttgtagtcgat"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3239..6925
                     /label="ADAR150cds"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3239..6925
                     /label="Translation 3239-6925"
                     /translation="LVNPRQGYSLSGYYTHPFQGYEHRQLRYQQPGPGSSPSSFLLKQIEFLKGQLPEAPVIGKQTPSLPPSLPGLRPRFPVLLASSTRGRQVDIRGVPRGVHLGSQGLQRGFQHPSPRGRSLPQRGVDCLSSHFQELSIYQDQEQRILKFLEELGEGKATTAHDLSGKLGTPKKEINRVLYSLAKKGKLQKEAGTPPLWKIAVSTQAWNQHSGVVRPDGHSQGAPNSDPSLEPEDRNSTSVSEDLLEPFIAVSAQAWNQHSGVVRPDSHSQGSPNSDPGLEPEDSNSTSALEDPLEFLDMAEIKEKICDYLFNVSDSSALNLAKNIGLTKARDINAVLIDMERQGDVYRQGTTPPIWHLTDKKRERMQIKRNTNSVPETAPAAIPETRRNAEFLTCNIPTSNASNNMVTTEKVENGQEPVIKLENRQEARPEPARLKPPVHYNGPSKAGYVDFENGQWATDDIPDDLNSIRAAPGEFRAIMEMPSFYSHGLPRCSPYKKLTECQLKNPISGLLEYAQFASQTCEFNMIEQSGPPHEPRFKFQVVINGREFPPAEAGSKKVAKQDAAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEKTAESQTPTPSATSFFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHEPKFQYCVAVGAQTFPSVSAPSKKVAKQMAAEEAMKALHGEATNSMASDNQPEGMISESLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTFHDQIAMLSHRCFNTLTNSFQPSLLGRKILAAIIMKKDSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCPVLV"
     primer          complement(3594..3613)
                     /label="XZp003.1"
                     /note="sequence: ATCAACACCTCTCTGTGGCA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3638..3838
                     /label="Z-DNA binding domain 1"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(3724..3745)
                     /label="ADAR1-p150-R1"
                     /note="sequence: CGGAGTCCCAAGTTTCCCAGAC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4118..4312
                     /label="Z-DNA binding domain 2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4247..4266)
                     /label="ADAR1-p150-R2"
                     /note="sequence: TCCCCCTGCCTTTCCATGTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4278..4297
                     /label="JK109_seq4_F"
                     /note="sequence: AAGGGACAACCCCTCCCATA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4313..4331
                     /label="XZp001.3"
                     /note="sequence: AAGAAGCGAGAGAGGATGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4325..4347)
                     /label="JK109_seq5_R"
                     /note="sequence: GTATTTCTCTTGATTTGCATCCT"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(4742..4761)
                     /label="ADAR1-p150-R3"
                     /note="sequence: CCGCTGATGGGGTTCTTCAG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4748..4954
                     /label="DRBM1"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          5022..5041
                     /label="JK109_seq3_F"
                     /note="sequence: AGAAGACTGCAGAGTCCCAG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    5081..5287
                     /label="DRBM2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(5367..5386)
                     /label="XZp002.1"
                     /note="sequence: CTTCCTGACCTTGTTGGGCA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(5374..5396)
                     /label="ADAR1-p150-R4"
                     /note="sequence: GCTCGCCAATCTTCCTGACCTTG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5417..5623
                     /label="DRBM3"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          5691..5710
                     /label="JK109_seq2_F"
                     /note="sequence: GAACTATGCTCCTCCTCTCA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          5762..5783
                     /label="XZp005.1"
                     /note="sequence: CATGACCAGATAGCCATGCTGA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5781..5797)
                     /label="XZp001.4"
                     /note="sequence: GAAGCACCGGTGGCTCA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          5839..5857
                     /label="ADAR1-p150-F7"
                     /note="sequence: CAAGATTCTGGCCGCCATC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          5879..5897
                     /label="ADAR1-core-seq-F"
                     /note="sequence: GACATGGGTGTCGTCGTCA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    5897..6904
                     /label="editase"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(5955..5975)
                     /label="ADAR1-p150-R5"
                     /note="sequence: CTGCATGGCAGTCATTGACAG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5976..5976
                     /label="E912A inactive"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          6158..6175
                     /label="MCP-ADAR-seq-F3"
                     /note="sequence: GACAAGTCCTGCAGCGAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          6309..6325
                     /label="MCP-ADAR1-seq-F2"
                     /note="sequence: ATGGCATTCGGCTCGGG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          6349..6369
                     /label="JK109_seq1_F"
                     /note="sequence: TAGTGACAAAATCCTACGCTG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          6360..6379
                     /label="ADAR1-p150-F6"
                     /note="sequence: TCCTACGCTGGAACGTGCTG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(6901..6925)
                     /label="EKo400.2"
                     /note="sequence: GACTAGTACTGGGCAGAGATAAAAG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(6923..6974)
                     /label="EKo574.2"
                     /note="sequence: gtttttgttcgggccctcctcgagcggcCTAATGGTGATGGTGATGGTGGAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    6926..6943
                     /label="His"
                     /ApEinfo_revcolor="#b111ee"
                     /ApEinfo_fwdcolor="#b111ee"
     CDS             6926..6946
                     /label="Translation 6926-6946"
                     /translation="HHHHHH*"
     primer          complement(6943..6959)
                     /label="EKo130.2"
                     /note="sequence: ctcctcgagcggcctaA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    6944..6946
                     /label="stop codon"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
     primer          complement(6947..6983)
                     /label="oLSM0065"
                     /note="sequence: ctgagatgagtttttgttcgggccctcctcgagcggc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(6948..6981)
                     /label="oLSM0043"
                     /note="sequence: gagatgagtttttgttcgggccctcctcgagcgg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(6949..6970)
                     /label="EKbR"
                     /note="sequence: ttgttcGGGCCctcctcgagcg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(6950..6966)
                     /label="CCp088 XhoI_myc-R"
                     /note="sequence: tcgggccctcctcgagc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(6951..6976)
                     /label="AP4_STOP_R"
                     /note="sequence: gagtttttgttcgggccctcctcgag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6953..6970)
                     /label="pNSK053"
                     /note="sequence: ttgttcgggccctcctcg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(6956..6974)
                     /label="YXo033.4"
                     /note="sequence: gtttttgttcgggccctcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(6956..6983)
                     /label="NK145-R-rtTA3G-CMV-ApaI"
                     /note="sequence: ctgagatgagtttttgttcgggccctcc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(6959..6986)
                     /label="NK64-RBPMS-partB-rev"
                     /note="sequence: cttctgagatgagtttttgttcgggccc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(6960..6980)
                     /label="RXR_CD8_R"
                     /note="sequence: agatgagtttttgTTCgggcc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6960..6981)
                     /label="pNK018"
                     /note="sequence: gagatgagtttttgttcGGGCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(6964..6989)
                     /label="C-epitope_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6964..6989)
                     /label="c-Myc_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             6965..6997
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    6997..6999
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             7010..7027
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(7013..7061)
                     /label="GRo015.3"
                     /note="sequence: gcacagtcgaggctgatcagcgggtttaaactcaatgatgattatgatg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    7028..7030
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(7031..7049)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(7043..7061)
                     /label="XZp086.2"
                     /note="sequence: gcacagtcgaggctgatca"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    7049..7263
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(7050..7067)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(7050..7085)
                     /label="NK-triggerEK0300-Sanger-r"
                     /note="sequence: caacagatggctggcaactagaaggcacagtcgagg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(7105..7124)
                     /label="XZ41-test-R1"
                     /note="sequence: CACCTTCCAGGGTCAAGGAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(7129..7148)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(7236..7263)
                     /label="oLSM0086.2"
                     /note="sequence: TCCCCAGCATGCCTGCTATTGTCTTCCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          7240..7258
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(7243..7260)
                     /label="EKo326.3"
                     /note="sequence: ccagcatgcctgctattg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(7243..7263)
                     /label="oLSM0086"
                     /note="sequence: TCCCCAGCATGCCTGCTATTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    7296..7319
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          7320..7338
                     /label="EKo293.3"
                     /note="sequence: cttctgaggcggaaagaac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(7398..7413)
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          7530..7548
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    7602..7649
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(7623..7650)
                     /label="EKo299.3"
                     /note="sequence: ggaagttcctatactttctagagaatag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            7657..8677
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          7885..7906
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          8605..8623
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_signal    8809..8939
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(8822..8847)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(8846..8865)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(8979..8995)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(8993..9012)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          9271..9290
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      9322..25
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(9442..9461)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     1201 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     1261 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     1321 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgtacggg
     1381 ccagatatac gcgttgacat tgattattga ctagttatta ttagtaatca attacggggt
     1441 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     1501 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     1561 taacgccaat agggactttc cattgacgtc aatgggtgga ctatttacgg taaactgccc
     1621 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     1681 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     1741 agtacatcta cgtattagtc atcgctatta ccatgggtcg aggtgagccc cacgttctgc
     1801 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttat
     1861 ttattttgtg cagcgatggg ggcggggggg gggggggcgc gcgccaggcg gggcggggcg
     1921 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
     1981 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
     2041 cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt gccccgctcc gcgccgcctc
     2101 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     2161 cccttctcct ccgggctgta attagcgctt ggtttattga cggctcgttt cttttctgtg
     2221 gctgcgtgaa agccttatag ggctccggga gggccctttg tgcggggggg agcggctcgg
     2281 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg
     2341 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc
     2401 gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc
     2461 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc
     2521 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2581 gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2641 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggccccgga
     2701 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     2761 agagggcgca gggacttcct ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg
     2821 ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     2881 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca gcctcggggc
     2941 tgccgcaggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     3001 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     3061 agctcctggg caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gcacgtgagg
     3121 accgggttta aacttaagct tcgccaccat ggggtcccgg gctgactaca aagaccatga
     3181 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagg ggcgcgccCT
     3241 AGTAAATCCG CGGCAGGGGT ATTCCCTCAG CGGATACTAC ACCCATCCAT TTCAAGGCTA
     3301 TGAGCACAGA CAGCTCAGAT ACCAGCAGCC TGGGCCAGGA TCTTCCCCCA GTAGTTTCCT
     3361 GCTTAAGCAA ATAGAATTTC TCAAGGGGCA GCTCCCAGAA GCACCGGTGA TTGGAAAGCA
     3421 GACACCGTCA CTGCCACCTT CCCTCCCAGG ACTCCGGCCA AGGTTTCCAG TACTACTTGC
     3481 CTCCAGTACC AGAGGCAGGC AAGTGGACAT CAGGGGTGTC CCCAGGGGCG TGCATCTCGG
     3541 AAGTCAGGGG CTCCAGAGAG GGTTCCAGCA TCCTTCACCA CGTGGCAGGA GTCTGCCACA
     3601 GAGAGGTGTT GATTGCCTTT CCTCACATTT CCAGGAACTG AGTATCTACC AAGATCAGGA
     3661 ACAAAGGATC TTAAAGTTCC TGGAAGAGCT TGGGGAAGGG AAGGCCACCA CAGCACATGA
     3721 TCTGTCTGGG AAACTTGGGA CTCCGAAGAA AGAAATCAAT CGAGTTTTAT ACTCCCTGGC
     3781 AAAGAAGGGC AAGCTACAGA AAGAGGCAGG AACACCCCCT TTGTGGAAAA TCGCGGTCTC
     3841 CACTCAGGCT TGGAACCAGC ACAGCGGAGT GGTAAGACCA GACGGTCATA GCCAAGGAGC
     3901 CCCAAACTCA GACCCGAGTT TGGAACCGGA AGACAGAAAC TCCACATCTG TCTCAGAAGA
     3961 TCTTCTTGAG CCTTTTATTG CAGTCTCAGC TCAGGCTTGG AACCAGCACA GCGGAGTGGT
     4021 AAGACCAGAC AGTCATAGCC AAGGATCCCC AAACTCAGAC CCAGGTTTGG AACCTGAAGA
     4081 CAGCAACTCC ACATCTGCCT TGGAAGATCC TCTTGAGTTT TTAGACATGG CCGAGATCAA
     4141 GGAGAAAATC TGCGACTATC TCTTCAATGT GTCTGACTCC TCTGCCCTGA ATTTGGCTAA
     4201 AAATATTGGC CTTACCAAGG CCCGAGATAT AAATGCTGTG CTAATTGACA TGGAAAGGCA
     4261 GGGGGATGTC TATAGACAAG GGACAACCCC TCCCATATGG CATTTGACAG ACAAGAAGCG
     4321 AGAGAGGATG CAAATCAAGA GAAATACGAA CAGTGTTCCT GAAACCGCTC CAGCTGCAAT
     4381 CCCTGAGACC AGAAGAAACG CAGAGTTCCT CACCTGTAAT ATACCCACAT CAAATGCCTC
     4441 AAATAACATG GTAACCACAG AAAAAGTGGA GAATGGGCAG GAACCTGTCA TAAAGTTAGA
     4501 AAACAGGCAA GAGGCCAGAC CAGAACCAGC AAGACTGAAA CCACCTGTTC ATTACAATGG
     4561 CCCCTCAAAA GCAGGGTATG TTGACTTTGA AAATGGCCAG TGGGCCACAG ATGACATCCC
     4621 AGATGACTTG AATAGTATCC GCGCAGCACC AGGTGAGTTT CGAGCCATCA TGGAGATGCC
     4681 CTCCTTCTAC AGTCATGGCT TGCCACGGTG TTCACCCTAC AAGAAACTGA CAGAGTGCCA
     4741 GCTGAAGAAC CCCATCAGCG GGCTGTTAGA ATATGCCCAG TTCGCTAGTC AAACCTGTGA
     4801 GTTCAACATG ATAGAGCAGA GTGGACCACC CCATGAACCT CGATTTAAAT TCCAGGTTGT
     4861 CATCAATGGC CGAGAGTTTC CCCCAGCTGA AGCTGGAAGC AAGAAAGTGG CCAAGCAGGA
     4921 TGCAGCTATG AAAGCCATGA CAATTCTGCT AGAGGAAGCC AAAGCCAAGG ACAGTGGAAA
     4981 ATCAGAAGAA TCATCCCACT ATTCCACAGA GAAAGAATCA GAGAAGACTG CAGAGTCCCA
     5041 GACCCCCACC CCTTCAGCCA CATCCTTCTT TTCTGGGAAG AGCCCCGTCA CCACACTGCT
     5101 TGAGTGTATG CACAAATTGG GGAACTCCTG CGAATTCCGT CTCCTGTCCA AAGAAGGCCC
     5161 TGCCCATGAA CCCAAGTTCC AATACTGTGT TGCAGTGGGA GCCCAAACTT TCCCCAGTGT
     5221 GAGTGCTCCC AGCAAGAAAG TGGCAAAGCA GATGGCCGCA GAGGAAGCCA TGAAGGCCCT
     5281 GCATGGGGAG GCGACCAACT CCATGGCTTC TGATAACCAG CCTGAAGGTA TGATCTCAGA
     5341 GTCACTTGAT AACTTGGAAT CCATGATGCC CAACAAGGTC AGGAAGATTG GCGAGCTCGT
     5401 GAGATACCTG AACACCAACC CTGTGGGTGG CCTTTTGGAG TACGCCCGCT CCCATGGCTT
     5461 TGCTGCTGAA TTCAAGTTGG TCGACCAGTC CGGACCTCCT CACGAGCCCA AGTTCGTTTA
     5521 CCAAGCAAAA GTTGGGGGTC GCTGGTTCCC AGCCGTCTGC GCACACAGCA AGAAGCAAGG
     5581 CAAGCAGGAA GCAGCAGATG CGGCTCTCCG TGTCTTGATT GGGGAGAACG AGAAGGCAGA
     5641 ACGCATGGGT TTCACAGAGG TAACCCCAGT GACAGGGGCC AGTCTCAGAA GAACTATGCT
     5701 CCTCCTCTCA AGGTCCCCAG AAGCACAGCC AAAGACACTC CCTCTCACTG GCAGCACCTT
     5761 CCATGACCAG ATAGCCATGC TGAGCCACCG GTGCTTCAAC ACTCTGACTA ACAGCTTCCA
     5821 GCCCTCCTTG CTCGGCCGCA AGATTCTGGC CGCCATCATT ATGAAAAAAG ACTCTGAGGA
     5881 CATGGGTGTC GTCGTCAGCT TGGGAACAGG GAATCGCTGT GTAAAAGGAG ATTCTCTCAG
     5941 CCTAAAAGGA GAAACTGTCA ATGACTGCCA TGCAGaAATA ATCTCCCGGA GAGGCTTCAT
     6001 CAGGTTTCTC TACAGTGAGT TAATGAAATA CAACTCCCAG ACTGCGAAGG ATAGTATATT
     6061 TGAACCTGCT AAGGGAGGAG AAAAGCTCCA AATAAAAAAG ACTGTGTCAT TCCATCTGTA
     6121 TATCAGCACT GCTCCGTGTG GAGATGGCGC CCTCTTTGAC AAGTCCTGCA GCGACCGTGC
     6181 TATGGAAAGC ACAGAATCCC GCCACTACCC TGTCTTCGAG AATCCCAAAC AAGGAAAGCT
     6241 CCGCACCAAG GTGGAGAACG GAGAAGGCAC AATCCCTGTG GAATCCAGTG ACATTGTGCC
     6301 TACGTGGGAT GGCATTCGGC TCGGGGAGAG ACTCCGTACC ATGTCCTGTA GTGACAAAAT
     6361 CCTACGCTGG AACGTGCTGG GCCTGCAAGG GGCACTGTTG ACCCACTTCC TGCAGCCCAT
     6421 TTATCTCAAA TCTGTCACAT TGGGTTACCT TTTCAGCCAA GGGCATCTGA CCCGTGCTAT
     6481 TTGCTGTCGT GTGACAAGAG ATGGGAGTGC ATTTGAGGAT GGACTACGAC ATCCCTTTAT
     6541 TGTCAACCAC CCCAAGGTTG GCAGAGTCAG CATATATGAT TCCAAAAGGC AATCCGGGAA
     6601 GACTAAGGAG ACAAGCGTCA ACTGGTGTCT GGCTGATGGC TATGACCTGG AGATCCTGGA
     6661 CGGTACCAGA GGCACTGTGG ATGGGCCACG GAATGAATTG TCCCGGGTCT CCAAAAAGAA
     6721 CATTTTTCTT CTATTTAAGA AGCTCTGCTC CTTCCGTTAC CGCAGGGATC TACTGAGACT
     6781 CTCCTATGGT GAGGCCAAGA AAGCTGCCCG TGACTACGAG ACGGCCAAGA ACTACTTCAA
     6841 AAAAGGCCTG AAGGATATGG GCTATGGGAA CTGGATTAGC AAACCCCAGG AGGAAAAGAA
     6901 CTTTTATCTC TGCCCAGTAC TAGTCCACCA TCACCATCAC CATTAGgccg ctcgaggagg
     6961 gcccgaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc atcatcataa
     7021 tcatcattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7081 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7141 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7201 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7261 ggatgcggtg ggctctatgg agatctatcg atgttctggg gatgcggtgg gctctatggc
     7321 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     7381 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7441 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7501 ccgtcaagct ctaaatcggg ggtcccttta gggttccgat ttagtgcttt acggcacctc
     7561 gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt
     7621 tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg
     7681 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     7741 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     7801 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     7861 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     7921 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     7981 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     8041 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     8101 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     8161 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     8221 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     8281 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     8341 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     8401 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     8461 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     8521 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     8581 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     8641 aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat
     8701 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     8761 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     8821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8941 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     9001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9781 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9961 ggatctcaag
//