LOCUS       EK0717_SFFV-mCherry-SGc 6728 bp ds-DNA     circular     11-APR-2023
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     primer          1..21
                     /label="FlpInF1"
                     /note="sequence: agtatctgctccctgcttgtg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          65..82
                     /label="FlpInF"
                     /note="sequence: aacaaggcaaggcttgac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          65..83
                     /label="EF1a_F"
                     /note="sequence: aacaaggcaaggcttgacc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          76..121
                     /label="EKo285.1"
                     /note="sequence: gcttgaccgacaattgccgataaaataaaagattttatttagtctc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(83..114)
                     /label="EKo695.2"
                     /note="sequence: aataaaatcttttattttatcggcaattgtcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        92..585
                     /label="SFFV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          93..167
                     /label="EWo118.11"
                     /note="sequence: cgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(94..171)
                     /label="GRo003.4"
                     /note="sequence: ctagcttgccataacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(97..171)
                     /label="EWo118.12"
                     /note="sequence: ctagcttgccataacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttatttt"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          98..167
                     /label="GRo003.3"
                     /note="sequence: aaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    160..160
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    188..188
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    266..266
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    363..363
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          472..500
                     /label="SSFV-NKseq-f"
                     /note="sequence: ccaatcagcctgcttctcgcttctgttcg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    504..520
                     /label="EKo438.IVT-1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          504..520
                     /label="EK_SFFV_F2"
                     /note="sequence: gcttctgcttcccgagc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          569..610
                     /label="oLSM0127-temp"
                     /note="sequence: agactgagtcgcccgggaagcttcgccaccATGGTGAGCAAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(572..601)
                     /label="EKo271.2"
                     /note="sequence: catggtggcgaagcttcccgggcgactcag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          572..607
                     /label="XZp054.1"
                     /note="sequence: ctgagtcgcccgggaagcttcgccaccATGGTGAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(574..601)
                     /label="GRo0112.2"
                     /note="sequence: catggtggcgaagcttcccgggcgactc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          575..613
                     /label="EKo412.2"
                     /note="sequence: agtcgcccgggaagcttcgccaccATGgtgagcaagggc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          576..601
                     /label="EK_SFFV_F"
                     /note="sequence: gtcgcccgggaagcttcgccaccatg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          586..601
                     /label="EKo271.3"
                     /note="sequence: aagcttcgccaccatg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          587..602
                     /label="EKo309.1"
                     /note="sequence: agcttcgccaccatgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          587..617
                     /label="EKo326.2"
                     /note="sequence: agcttcgccaccATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    591..598
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          593..610
                     /label="YXo023.1"
                     /note="sequence: gccaccatgGTGAGCAAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(593..610)
                     /label="YXo021.2"
                     /note="sequence: CTTGCTCACCATggtggc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(596..611)
                     /label="Cit_1"
                     /note="sequence: ccttgctcaccatGGT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             599..601
                     /label="Translation 599-601"
                     /translation="M"
     primer          599..615
                     /label="XZp042.5"
                     /note="sequence: atggtgagcaagggcga"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          599..616
                     /label="pNSK019"
                     /note="sequence: ATGGTGAGCAAGGGCGAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             602..1309
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff4273"
                     /ApEinfo_fwdcolor="#ff4273"
     CDS             602..1309
                     /label="Translation 602-1309"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKS"
     primer          complement(605..619)
                     /label="Che_5"
                     /note="sequence: ctcctcgcccttgct"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(637..654)
                     /label="che_8"
                     /note="sequence: aagcgcatgaactccttg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(637..654)
                     /label="Che_8"
                     /note="sequence: aagcgcatgaactccttg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          683..700
                     /label="YX023-seq-1"
                     /note="sequence: GGCCACGAGTTCGAGATC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(737..753)
                     /label="EKo230.1"
                     /note="sequence: TTCAGCTTGgcggtctg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          800..818
                     /label="mChe_4"
                     /note="sequence: cctcagttcatgtacggct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(805..822)
                     /label="mChe_1"
                     /note="sequence: ttggagccgtacatgaac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(867..881)
                     /label="mChe_3"
                     /note="sequence: CCTCGGGGAAGGACA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(901..920)
                     /label="pNSK093"
                     /note="sequence: cgccgtcctcgaagttcatc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(954..969)
                     /label="Che_6"
                     /note="sequence: ATGAACTCGCCGTCCT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1026..1047
                     /label="YX025-seq-2"
                     /note="sequence: agaagaccatgggctgggaggc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1159..1180
                     /label="NK-cherry-seq"
                     /note="sequence: GGCCAAGAAGCCCGTGCAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1159..1180
                     /label="NK-cherry-seq"
                     /note="sequence: GGCCAAGAAGCCCGTGCAGCTG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1185..1203)
                     /label="XZp081.3"
                     /note="sequence: ATGTTGACGTTGTAGGCGC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1202..1223
                     /label="YX017-seq-1"
                     /note="sequence: atcaagttggacatcacctccc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1215..1230
                     /label="mChe_2"
                     /note="sequence: TCACCTCCCACAACGA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1270..1284
                     /label="Che_7"
                     /note="sequence: CCGCCACTCCACCGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1284..1299
                     /label="XZp073.1"
                     /note="sequence: GCGGCATGGACGAGCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1286..1306)
                     /label="pNSK091"
                     /note="sequence: cttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1288..1308
                     /label="Che9"
                     /note="sequence: catggacgagctgtacaagAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1288..1309
                     /label="JSo042_sensor_qPCR_F1"
                     /note="sequence: CATGGACGAGCTGTACAAGAGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1288..1327)
                     /label="EKo408.1"
                     /note="sequence: TCTGGCTTCcaacgcgtcGCTCTTGTACAGCTCGTCCATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1290..1309)
                     /label="XZp092.2"
                     /note="sequence: GCTCTTGTACAGCTCGTCCA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1310..1318
                     /label="Translation 1310-1318"
                     /translation="DAL"
     primer          1310..1327
                     /label="pNSK124"
                     /note="sequence: gacgcgttgGAAGCCAGA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1313..1330
                     /label="pNSK131"
                     /note="sequence: gcgttgGAAGCCAGACAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1319..1336
                     /label="EKo260.2"
                     /note="sequence: GAAGCCAGACACAAACAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1319..1417
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1319..1417
                     /label="Translation 1319-1417"
                     /translation="EARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP"
     primer          1343..1360
                     /label="Pepper-seq-F"
                     /note="sequence: GTGGCTCCGGTGAAACAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1371..1389
                     /label="JSo044_sensor_qPCR_F2_OLD"
                     /note="sequence: TTGACCTTCTGAAGCTGGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1380..1396)
                     /label="XZ071-test-R"
                     /note="sequence: GTCTCCCGCCAGCTTCA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1402..1417)
                     /label="JMo3.2"
                     /note="sequence: GGGACCGGGGTTGGAC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1403..1417
                     /label="EK-5SGF"
                     /note="sequence: TCCAACCCCGGTCCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1404..1418
                     /label="XZp087.1"
                     /note="sequence: CCAACCCCGGTCCCg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1413..1429
                     /label="XZp082.1"
                     /note="sequence: GTCCCggtaccggatcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1418..1423
                     /label="Translation 1418-1423"
                     /translation="GT"
     misc_feature    1424..1429
                     /label="bamhi site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1424..1429
                     /label="Translation 1424-1429"
                     /translation="GS"
     primer          1425..1495
                     /label="pNSK384"
                     /note="sequence: gatccACCAAGTCGACACGGATGAGTGTTTCCTTCTATCCGCGTCATTGTacCATTtAGGTGGGTGGAATG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1429..1499)
                     /label="pNSK385"
                     /note="sequence: TTAGCATTCCACCCACCTaAATGgtACAATGACGCGGATAGAAGGAAACACTCATCCGTGTCGACTTGGTg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1430..1474
                     /label="one side"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1430..1564
                     /label="s70587"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1430..1564
                     /label="Translation 1430-1564"
                     /translation="TKSTRMSVSFYPRHCTI*VGGMLNPTYPDAWALRTDRPLVQIESY"
     misc_feature    1455..1474
                     /label="oh"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1477..1494
                     /label="stem"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1477..1517
                     /label="GLURB 18bp"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1495..1499
                     /label="loop"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1496..1565
                     /label="pNSK386"
                     /note="sequence: CTAAATCCCACCTACCCaGATGccTgGGCCTTACGTACCGATAGACCACTGGTGCAAATAGAGAGCTATa"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1500..1517
                     /label="stem"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1500..1569)
                     /label="pNSK387"
                     /note="sequence: ccggtATAGCTCTCTATTTGCACCAGTGGTCTATCGGTACGTAAGGCCcAggCATCtGGGTAGGTGGGAT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1520..1564
                     /label="other side"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1527..1571
                     /label="rec10-rev-f2"
                     /note="sequence: cacaaCAGAAACGTCACGTCTGGCGacatgaggattacccatgta"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1565..1570
                     /label="bshti site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1565..1570
                     /label="Translation 1565-1570"
                     /translation="TG"
     primer          complement(1565..1583)
                     /label="EKo706.3"
                     /note="sequence: GTGCCTCactagtaccggt"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1571..1576
                     /label="Translation 1571-1576"
                     /translation="TS"
     primer          1577..1592
                     /label="EKo242.3"
                     /note="sequence: GAGGCACGGCATAAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1577..1592)
                     /label="EK-3SGR"
                     /note="sequence: GCTTATGCCGTGCCTC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1577..1675
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1577..1675
                     /label="Translation 1577-1675"
                     /translation="EARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP"
     primer          complement(1607..1630)
                     /label="JSo045_sensor_qPCR_R2"
                     /note="sequence: GAAGTTGAGTGTTTGCTTTACTGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1650..1680)
                     /label="oLSM0052"
                     /note="sequence: CTCGAGGGTCCTGGATTAGATTCCACATCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1652..1675)
                     /label="pNSK126"
                     /note="sequence: GGGTCCTGGATTAGATTCCACATC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1655..1675)
                     /label="EKo222.1"
                     /note="sequence: AGGTCCTGGATTAGATTCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1655..1675)
                     /label="EKo387.3"
                     /note="sequence: GGGTCCTGGATTAGATTCCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1664..1700
                     /label="EKo409.1"
                     /note="sequence: AATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1676..1681
                     /label="Translation 1676-1681"
                     /translation="SS"
     primer          1682..1700
                     /label="EKo147.1"
                     /note="sequence: GTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1682..1709
                     /label="oLSM0117"
                     /note="sequence: GTCTCAAAGGGCGAGGAACTTTTTACGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1682..2395
                     /label="GFP"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1682..2395
                     /label="Translation 1682-2395"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer          complement(1715..1735)
                     /label="GFP5"
                     /note="sequence: CAATTCGACGAGGATCGGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1719..1738)
                     /label="JSo043_sensor_qPCR_R1"
                     /note="sequence: GTCCAATTCGACGAGGATCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1744..1773)
                     /label="NK-gfp-seq-r"
                     /note="sequence: CCACTAACTGAGAATTTATGCCCATTAACG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1830..1848
                     /label="pNSK084"
                     /note="sequence: CCGGAAAATTGCCAGTGCC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1834..1849
                     /label="EWo208.1"
                     /note="sequence: AAAATTGCCAGTGCCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1847..1863)
                     /label="CCp019-repGFPrev"
                     /note="sequence: ACGAGAGTAGGCCACGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1938..1954)
                     /label="EKo224.2"
                     /note="sequence: ACCTTCAGGCATAGCGG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2046..2066
                     /label="pPYK028"
                     /note="sequence: GGATCGAACTGAAGGGCATAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2050..2075
                     /label="YX017-seq-2"
                     /note="sequence: CGAACTGAAGGGCATAGATTTTAAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2323..2342
                     /label="GFP6"
                     /note="sequence: ACGGGATCACATGGTCCTTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2364..2398)
                     /label="CCp024-GFP-r"
                     /note="sequence: CTACTTATACAGTTCGTCCATCCCGAGTGTTATCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2374..2395
                     /label="EKoCC34.2"
                     /note="sequence: CGGGATGGACGAACTGTATAAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2374..2395)
                     /label="EKo460.1"
                     /note="sequence: CTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2374..2398)
                     /label="JSo033_sensorGFP_R"
                     /note="sequence: ctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2374..2398)
                     /label="pNSK025"
                     /note="sequence: ctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2374..2409)
                     /label="YXo007.2"
                     /note="sequence: ggccctccggcctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2396..2398
                     /label="Translation 2396-2398"
                     /translation="*"
     primer          complement(2406..2441)
                     /label="EKo438.3"
                     /note="sequence: GGTGTCTGAGACGTACCCTGATGGTGTACGTgggcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2410..2445
                     /label="SB011.1"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2410..2446
                     /label="EKo438.1"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          2410..2477
                     /label="Rec14-US-3xMS2nbt-f"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2411..2476
                     /label="3xMS2 SGR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2420..2423
                     /label="loop MS2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2442..2445
                     /label="loop MS2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2442..2476)
                     /label="EKo438.4"
                     /note="sequence: ATGGTACGCTGATGCTGTACCAGACAGACCCTGAT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2446..2477
                     /label="SB011.2"
                     /note="sequence: GGGTCTGTCTGGTACAGCATCAGCGTACCATg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2447..2480
                     /label="EKo438.2"
                     /note="sequence: GGTCTGTCTGGTACAGCATCAGCGTACCATggcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2464..2467
                     /label="loop MS2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2477..2480
                     /label="overhang"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2477..2497)
                     /label="RXR_CD8_R"
                     /note="sequence: agatgagtttttgTTCgggcc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2477..2498)
                     /label="pNK018"
                     /note="sequence: gagatgagtttttgttcGGGCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2481..2506)
                     /label="c-Myc_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2481..2506)
                     /label="C-epitope_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             2482..2514
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2514..2516
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             2527..2544
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(2530..2578)
                     /label="GRo015.3"
                     /note="sequence: gcacagtcgaggctgatcagcgggtttaaactcaatgatgattatgatg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2545..2547
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(2548..2566)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2560..2578)
                     /label="XZp086.2"
                     /note="sequence: gcacagtcgaggctgatca"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2562..2579)
                     /label="pNSK316"
                     /note="sequence: ggcacagtcgaggctgat"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     polyA_signal    2566..2780
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(2567..2584)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2567..2602)
                     /label="NK-triggerEK0300-Sanger-r"
                     /note="sequence: caacagatggctggcaactagaaggcacagtcgagg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2622..2641)
                     /label="XZ41-test-R1"
                     /note="sequence: CACCTTCCAGGGTCAAGGAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2646..2665)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2753..2780)
                     /label="oLSM0086.2"
                     /note="sequence: TCCCCAGCATGCCTGCTATTGTCTTCCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2757..2775
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2760..2777)
                     /label="EKo326.3"
                     /note="sequence: ccagcatgcctgctattg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2760..2780)
                     /label="oLSM0086"
                     /note="sequence: TCCCCAGCATGCCTGCTATTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    2813..2836
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          2837..2855
                     /label="EKo293.3"
                     /note="sequence: cttctgaggcggaaagaac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2915..2930)
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3047..3065
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3119..3166
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(3137..3168)
                     /label="pNSK331"
                     /note="sequence: aggaagttcctatactttctagagaataggaa"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3140..3167)
                     /label="EKo299.3"
                     /note="sequence: ggaagttcctatactttctagagaatag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            3174..4194
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3402..3423
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4122..4140
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4325..4397)
                     /label="NK174-tile1-r"
                     /note="sequence: AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    4326..4456
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(4339..4364)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4350..4407)
                     /label="NK174-tile2-r"
                     /note="sequence: GCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4363..4382)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4496..4512)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4510..4529)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4558..4580)
                     /label="An-o17 U6_3MS2_Cre_seq"
                     /note="sequence: ccaggctttacactttatgcttc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4788..4807
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4839..4863
                     /label="oLSM-pUC_ori_F"
                     /note="sequence: atgtgagcaaaaggccagcaaaagg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4839..4863)
                     /label="oLSM-pUC_ori_R"
                     /note="sequence: ccttttgctggccttttgctcacat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      4839..5512
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(4959..4978)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(5657..6517)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             complement(5657..6517)
                     /label="Translation 6517-5657"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer          5777..5796
                     /label="pNSK334"
                     /note="sequence: cagtgctgcaatgataccgc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(5782..5800)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(6490..6515)
                     /label="oLSM0054"
                     /note="sequence: gagtattcaacatttccgtgtcgccc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          6490..6521
                     /label="oLSM0053"
                     /note="sequence: gggcgacacggaaatgttgaatactcatactc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6518..6616)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          6612..6628
                     /label="AmpR_seq_F"
                     /note="sequence: ggggttccgcgcacatt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
       61 ctacaacaag gcaaggcttg accgacaatt gccgataaaa taaaagattt tatttagtct
      121 ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta gctgcagtaa
      181 cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
      241 gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg agcagtttcg
      301 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
      361 gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
      421 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
      481 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
      541 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgggaagct tcgccaccAT
      601 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
      661 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
      721 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
      781 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
      841 CGCCGACATT CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
      901 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
      961 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     1021 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     1081 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     1141 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
     1201 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     1261 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAGCg acgcgttgGA
     1321 AGCCAGACAC AAACAGAAAA TTGTGGCTCC GGTGAAACAG ACTTTGAATT TTGACCTTCT
     1381 GAAGCTGGCG GGAGACGTCG AGTCCAACCC CGGTCCCggt accggatccA CCAAGTCGAC
     1441 ACGGATGAGT GTTTCCTTCT ATCCGCGTCA TTGTacCATT tAGGTGGGTG GAATGCTAAA
     1501 TCCCACCTAC CCaGATGccT gGGCCTTACG TACCGATAGA CCACTGGTGC AAATAGAGAG
     1561 CTATaccggt actagtGAGG CACGGCATAA GCAAAAGATC GTAGCCCCAG TAAAGCAAAC
     1621 ACTCAACTTC GATCTACTCA AACTCGCAGG TGATGTGGAA TCTAATCCAG GACCCTCGAG
     1681 TGTCTCAAAG GGCGAGGAAC TTTTTACGGG CGTTGTTCCG ATCCTCGTCG AATTGGACGG
     1741 GGACGTTAAT GGGCATAAAT TCTCAGTTAG TGGTGAGGGT GAGGGGGACG CGACATATGG
     1801 GAAACTTACG CTGAAGTTCA TATGTACCAC CGGAAAATTG CCAGTGCCGT GGCCTACTCT
     1861 CGTCACCACC CTGACATATG GTGTTCAGTG TTTTAGCCGG TATCCAGACC ATATGAAACA
     1921 GCACGATTTT TTTAAATCCG CTATGCCTGA AGGTTACGTA CAGGAGCGAA CGATATTTTT
     1981 TAAAGACGAC GGAAACTACA AAACTAGAGC CGAGGTAAAA TTCGAGGGTG ACACTCTCGT
     2041 GAACCGGATC GAACTGAAGG GCATAGATTT TAAGGAGGAT GGCAATATAC TGGGCCACAA
     2101 GCTCGAATAC AACTATAATT CTCATAATGT CTATATCATG GCGGACAAAC AGAAAAACGG
     2161 CATCAAGGTA AACTTTAAAA TCCGACACAA TATTGAGGAC GGCTCTGTCC AGCTTGCGGA
     2221 CCATTACCAA CAGAACACGC CGATAGGGGA TGGTCCAGTA CTTCTCCCTG ATAATCACTA
     2281 TTTGTCTACT CAGTCAGCTC TTAGCAAGGA CCCAAACGAA AAACGGGATC ACATGGTCCT
     2341 TCTTGAGTTC GTCACTGCGG CTGGGATAAC ACTCGGGATG GACGAACTGT ATAAGtaggc
     2401 cggagggccc ACGTACACCA TCAGGGTACG TCTCAGACAC CATCAGGGTC TGTCTGGTAC
     2461 AGCATCAGCG TACCATggcc cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc
     2521 gtcgaccatc atcataatca tcattgagtt taaacccgct gatcagcctc gactgtgcct
     2581 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2641 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2701 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2761 aatagcaggc atgctgggga tgcggtgggc tctatggaga tctatcgatg ttctggggat
     2821 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     2881 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3001 acgttcgccg gctttccccg tcaagctcta aatcgggggt ccctttaggg ttccgattta
     3061 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga
     3121 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc
     3181 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     3241 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     3301 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     3361 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     3421 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     3481 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     3541 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     3601 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     3661 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     3721 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     3781 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     3841 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     3901 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     3961 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     4021 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     4081 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     4141 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg
     4201 tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     4261 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     4321 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4441 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     4501 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4561 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     4621 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     4681 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4741 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4801 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4861 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4921 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4981 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5041 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5101 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5161 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5221 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5281 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5341 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5401 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5461 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5521 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5581 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5641 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5701 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5761 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5821 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5881 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5941 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6001 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6061 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6121 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6181 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6241 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6301 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6361 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6421 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6481 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6541 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6601 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     6661 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     6721 gttaagcc
//