LOCUS       EK0718_SFFV-mCherry-SGc 6719 bp ds-DNA     circular     11-APR-2023
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     primer          1..21
                     /label="FlpInF1"
                     /note="sequence: agtatctgctccctgcttgtg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          65..82
                     /label="FlpInF"
                     /note="sequence: aacaaggcaaggcttgac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          65..83
                     /label="EF1a_F"
                     /note="sequence: aacaaggcaaggcttgacc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          76..121
                     /label="EKo285.1"
                     /note="sequence: gcttgaccgacaattgccgataaaataaaagattttatttagtctc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(83..114)
                     /label="EKo695.2"
                     /note="sequence: aataaaatcttttattttatcggcaattgtcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        92..585
                     /label="SFFV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          93..167
                     /label="EWo118.11"
                     /note="sequence: cgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(94..171)
                     /label="GRo003.4"
                     /note="sequence: ctagcttgccataacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(97..171)
                     /label="EWo118.12"
                     /note="sequence: ctagcttgccataacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttatttt"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          98..167
                     /label="GRo003.3"
                     /note="sequence: aaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    160..160
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    188..188
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    266..266
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    363..363
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          472..500
                     /label="SSFV-NKseq-f"
                     /note="sequence: ccaatcagcctgcttctcgcttctgttcg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    504..520
                     /label="EKo438.IVT-1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          504..520
                     /label="EK_SFFV_F2"
                     /note="sequence: gcttctgcttcccgagc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          569..610
                     /label="oLSM0127-temp"
                     /note="sequence: agactgagtcgcccgggaagcttcgccaccATGGTGAGCAAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(572..601)
                     /label="EKo271.2"
                     /note="sequence: catggtggcgaagcttcccgggcgactcag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          572..607
                     /label="XZp054.1"
                     /note="sequence: ctgagtcgcccgggaagcttcgccaccATGGTGAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(574..601)
                     /label="GRo0112.2"
                     /note="sequence: catggtggcgaagcttcccgggcgactc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          575..613
                     /label="EKo412.2"
                     /note="sequence: agtcgcccgggaagcttcgccaccATGgtgagcaagggc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          576..601
                     /label="EK_SFFV_F"
                     /note="sequence: gtcgcccgggaagcttcgccaccatg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          586..601
                     /label="EKo271.3"
                     /note="sequence: aagcttcgccaccatg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          587..602
                     /label="EKo309.1"
                     /note="sequence: agcttcgccaccatgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          587..617
                     /label="EKo326.2"
                     /note="sequence: agcttcgccaccATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    591..598
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          593..610
                     /label="YXo023.1"
                     /note="sequence: gccaccatgGTGAGCAAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(593..610)
                     /label="YXo021.2"
                     /note="sequence: CTTGCTCACCATggtggc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(596..611)
                     /label="Cit_1"
                     /note="sequence: ccttgctcaccatGGT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             599..601
                     /label="Translation 599-601"
                     /translation="M"
     primer          599..615
                     /label="XZp042.5"
                     /note="sequence: atggtgagcaagggcga"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          599..616
                     /label="pNSK019"
                     /note="sequence: ATGGTGAGCAAGGGCGAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             602..1309
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff4273"
                     /ApEinfo_fwdcolor="#ff4273"
     CDS             602..1309
                     /label="Translation 602-1309"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKS"
     primer          complement(605..619)
                     /label="Che_5"
                     /note="sequence: ctcctcgcccttgct"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(637..654)
                     /label="Che_8"
                     /note="sequence: aagcgcatgaactccttg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(637..654)
                     /label="che_8"
                     /note="sequence: aagcgcatgaactccttg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          683..700
                     /label="YX023-seq-1"
                     /note="sequence: GGCCACGAGTTCGAGATC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(737..753)
                     /label="EKo230.1"
                     /note="sequence: TTCAGCTTGgcggtctg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          800..818
                     /label="mChe_4"
                     /note="sequence: cctcagttcatgtacggct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(805..822)
                     /label="mChe_1"
                     /note="sequence: ttggagccgtacatgaac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(867..881)
                     /label="mChe_3"
                     /note="sequence: CCTCGGGGAAGGACA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(901..920)
                     /label="pNSK093"
                     /note="sequence: cgccgtcctcgaagttcatc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(954..969)
                     /label="Che_6"
                     /note="sequence: ATGAACTCGCCGTCCT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1026..1047
                     /label="YX025-seq-2"
                     /note="sequence: agaagaccatgggctgggaggc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1159..1180
                     /label="NK-cherry-seq"
                     /note="sequence: GGCCAAGAAGCCCGTGCAGCTG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1159..1180
                     /label="NK-cherry-seq"
                     /note="sequence: GGCCAAGAAGCCCGTGCAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1185..1203)
                     /label="XZp081.3"
                     /note="sequence: ATGTTGACGTTGTAGGCGC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1202..1223
                     /label="YX017-seq-1"
                     /note="sequence: atcaagttggacatcacctccc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1215..1230
                     /label="mChe_2"
                     /note="sequence: TCACCTCCCACAACGA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1270..1284
                     /label="Che_7"
                     /note="sequence: CCGCCACTCCACCGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1284..1299
                     /label="XZp073.1"
                     /note="sequence: GCGGCATGGACGAGCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1286..1306)
                     /label="pNSK091"
                     /note="sequence: cttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1288..1308
                     /label="Che9"
                     /note="sequence: catggacgagctgtacaagAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1288..1309
                     /label="JSo042_sensor_qPCR_F1"
                     /note="sequence: CATGGACGAGCTGTACAAGAGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1288..1327)
                     /label="EKo408.1"
                     /note="sequence: TCTGGCTTCcaacgcgtcGCTCTTGTACAGCTCGTCCATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1290..1309)
                     /label="XZp092.2"
                     /note="sequence: GCTCTTGTACAGCTCGTCCA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1310..1318
                     /label="Translation 1310-1318"
                     /translation="DAL"
     primer          1310..1327
                     /label="pNSK124"
                     /note="sequence: gacgcgttgGAAGCCAGA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1313..1330
                     /label="pNSK131"
                     /note="sequence: gcgttgGAAGCCAGACAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1319..1336
                     /label="EKo260.2"
                     /note="sequence: GAAGCCAGACACAAACAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1319..1417
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1319..1417
                     /label="Translation 1319-1417"
                     /translation="EARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP"
     primer          1343..1360
                     /label="Pepper-seq-F"
                     /note="sequence: GTGGCTCCGGTGAAACAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1371..1389
                     /label="JSo044_sensor_qPCR_F2_OLD"
                     /note="sequence: TTGACCTTCTGAAGCTGGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1380..1396)
                     /label="XZ071-test-R"
                     /note="sequence: GTCTCCCGCCAGCTTCA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1402..1417)
                     /label="JMo3.2"
                     /note="sequence: GGGACCGGGGTTGGAC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1402..1439)
                     /label="EKo242.1"
                     /note="sequence: TCGACTTGGTggatccggtaccGGGACCGGGGTTGGAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1402..1474
                     /label="EKo714.1"
                     /note="sequence: GTCCAACCCCGGTCCCggtaccggatccACCAAGTCGACACGGATGAGTGTTTCCTTCTATCCGCGTCATTGT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1403..1417
                     /label="EK-5SGF"
                     /note="sequence: TCCAACCCCGGTCCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1404..1418
                     /label="XZp087.1"
                     /note="sequence: CCAACCCCGGTCCCg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1413..1429
                     /label="XZp082.1"
                     /note="sequence: GTCCCggtaccggatcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1418..1423
                     /label="Translation 1418-1423"
                     /translation="GT"
     CDS             1424..1429
                     /label="Translation 1424-1429"
                     /translation="GS"
     primer          1425..1466
                     /label="JS1_pJS001_REssrA_F1"
                     /note="sequence: gatccACCAAGTCGACACGGATGAGTGTTTCCTTCTATCCGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1425..1470
                     /label="EKo209.1"
                     /note="sequence: gatccACCAAGTCGACACGGATGAGTGTTTCCTTCTATCCGCGTCA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1429..1470)
                     /label="JS2_pJS001_REssrA_R1"
                     /note="sequence: TGACGCGGATAGAAGGAAACACTCATCCGTGTCGACTTGGTg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1429..1473
                     /label="EKo208.4"
                     /note="sequence: cACCAAGTCGACACGGATGAGTGTTTCCTTCTATCCGCGTCATTG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1429..1474)
                     /label="EKo209.3"
                     /note="sequence: ACAATGACGCGGATAGAAGGAAACACTCATCCGTGTCGACTTGGTg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1430..1449
                     /label="JSo044_sensor_qPCR_F2"
                     /note="sequence: ACCAAGTCGACACGGATGAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1430..1474
                     /label="one side"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1430..1555
                     /label="s70587"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1430..1555
                     /label="Translation 1430-1555"
                     /translation="TKSTRMSVSFYPRHCMRRPWTGS*PSVWALRTDRPLVQIESY"
     primer          complement(1455..1530)
                     /label="EKo718.1"
                     /note="sequence: CTATCGGTACGTAAGGCCcAAACAGACGGCTATGAACCAGTCCATGGCCGTCGCATACAATGACGCGGATAGAAGG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1475..1510
                     /label="GABRA3-derived stemloop"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1476..1476
                     /label="bulge"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1485..1485
                     /label="C opposite A"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1491..1494
                     /label="loop"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1500..1500
                     /label="editing target"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1507..1507
                     /label="bulge"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1511..1530
                     /label="oh"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1511..1555
                     /label="other side"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1511..1560)
                     /label="EKo233.4"
                     /note="sequence: ccggtATAGCTCTCTATTTGCACCAGTGGTCTATCGGTACGTAAGGCCcA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1511..1583
                     /label="EKo717.2"
                     /note="sequence: TgGGCCTTACGTACCGATAGACCACTGGTGCAAATAGAGAGCTATaccggtactagtGAGGCACGGCATAAGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1513..1573)
                     /label="EKo702.4"
                     /note="sequence: TGCCTCactagtaccggtATAGCTCTCTATTTGCACCAGTGGTCTATCGGTACGTAAGGCC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1515..1556
                     /label="EKo656.3"
                     /note="sequence: CCTTACGTACCGATAGACCACTGGTGCAAATAGAGAGCTATa"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1518..1562
                     /label="rec10-rev-f2"
                     /note="sequence: cacaaCAGAAACGTCACGTCTGGCGacatgaggattacccatgta"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1519..1556
                     /label="JSo022_REtgrA_R1"
                     /note="sequence: ACGTACCGATAGACCACTGGTGCAAATAGAGAGCTATa"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1519..1560)
                     /label="EKo678.1"
                     /note="sequence: ccggtATAGCTCTCTATTTGCACCAGTGGTCTATCGGTACGT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1523..1560)
                     /label="JSo021_REtgrA_F1"
                     /note="sequence: ccggtATAGCTCTCTATTTGCACCAGTGGTCTATCGGT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1532..1577)
                     /label="YXo023.2"
                     /note="sequence: GCCGTGCCTCactagtaccggtATAGCTCTCTATTTGCACCAGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1538..1583
                     /label="oLSM021"
                     /note="sequence: GTGCAAATAGAGAGCTATaccggtactagtGAGGCACGGCATAAGC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1556..1561
                     /label="Translation 1556-1561"
                     /translation="TG"
     primer          complement(1556..1574)
                     /label="EKo706.3"
                     /note="sequence: GTGCCTCactagtaccggt"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1562..1567
                     /label="Translation 1562-1567"
                     /translation="TS"
     primer          1568..1583
                     /label="EKo242.3"
                     /note="sequence: GAGGCACGGCATAAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1568..1583)
                     /label="EK-3SGR"
                     /note="sequence: GCTTATGCCGTGCCTC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1568..1666
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1568..1666
                     /label="Translation 1568-1666"
                     /translation="EARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP"
     primer          complement(1598..1621)
                     /label="JSo045_sensor_qPCR_R2"
                     /note="sequence: GAAGTTGAGTGTTTGCTTTACTGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1641..1671)
                     /label="oLSM0052"
                     /note="sequence: CTCGAGGGTCCTGGATTAGATTCCACATCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1643..1666)
                     /label="pNSK126"
                     /note="sequence: GGGTCCTGGATTAGATTCCACATC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1646..1666)
                     /label="EKo387.3"
                     /note="sequence: GGGTCCTGGATTAGATTCCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1646..1666)
                     /label="EKo222.1"
                     /note="sequence: AGGTCCTGGATTAGATTCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1655..1691
                     /label="EKo409.1"
                     /note="sequence: AATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1667..1672
                     /label="Translation 1667-1672"
                     /translation="SS"
     primer          1673..1691
                     /label="EKo147.1"
                     /note="sequence: GTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1673..1700
                     /label="oLSM0117"
                     /note="sequence: GTCTCAAAGGGCGAGGAACTTTTTACGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1673..2386
                     /label="GFP"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1673..2386
                     /label="Translation 1673-2386"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer          complement(1706..1726)
                     /label="GFP5"
                     /note="sequence: CAATTCGACGAGGATCGGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1710..1729)
                     /label="JSo043_sensor_qPCR_R1"
                     /note="sequence: GTCCAATTCGACGAGGATCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1735..1764)
                     /label="NK-gfp-seq-r"
                     /note="sequence: CCACTAACTGAGAATTTATGCCCATTAACG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1821..1839
                     /label="pNSK084"
                     /note="sequence: CCGGAAAATTGCCAGTGCC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1825..1840
                     /label="EWo208.1"
                     /note="sequence: AAAATTGCCAGTGCCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1838..1854)
                     /label="CCp019-repGFPrev"
                     /note="sequence: ACGAGAGTAGGCCACGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1929..1945)
                     /label="EKo224.2"
                     /note="sequence: ACCTTCAGGCATAGCGG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2037..2057
                     /label="pPYK028"
                     /note="sequence: GGATCGAACTGAAGGGCATAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2041..2066
                     /label="YX017-seq-2"
                     /note="sequence: CGAACTGAAGGGCATAGATTTTAAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2314..2333
                     /label="GFP6"
                     /note="sequence: ACGGGATCACATGGTCCTTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2355..2389)
                     /label="CCp024-GFP-r"
                     /note="sequence: CTACTTATACAGTTCGTCCATCCCGAGTGTTATCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2365..2386
                     /label="EKoCC34.2"
                     /note="sequence: CGGGATGGACGAACTGTATAAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2365..2386)
                     /label="EKo460.1"
                     /note="sequence: CTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2365..2389)
                     /label="JSo033_sensorGFP_R"
                     /note="sequence: ctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2365..2389)
                     /label="pNSK025"
                     /note="sequence: ctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2365..2400)
                     /label="YXo007.2"
                     /note="sequence: ggccctccggcctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2387..2389
                     /label="Translation 2387-2389"
                     /translation="*"
     primer          complement(2397..2432)
                     /label="EKo438.3"
                     /note="sequence: GGTGTCTGAGACGTACCCTGATGGTGTACGTgggcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2401..2436
                     /label="SB011.1"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2401..2437
                     /label="EKo438.1"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          2401..2468
                     /label="Rec14-US-3xMS2nbt-f"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2402..2467
                     /label="3xMS2 SGR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2411..2414
                     /label="loop MS2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2433..2436
                     /label="loop MS2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2433..2467)
                     /label="EKo438.4"
                     /note="sequence: ATGGTACGCTGATGCTGTACCAGACAGACCCTGAT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2437..2468
                     /label="SB011.2"
                     /note="sequence: GGGTCTGTCTGGTACAGCATCAGCGTACCATg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2438..2471
                     /label="EKo438.2"
                     /note="sequence: GGTCTGTCTGGTACAGCATCAGCGTACCATggcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2455..2458
                     /label="loop MS2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2468..2471
                     /label="overhang"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2468..2488)
                     /label="RXR_CD8_R"
                     /note="sequence: agatgagtttttgTTCgggcc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2468..2489)
                     /label="pNK018"
                     /note="sequence: gagatgagtttttgttcGGGCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2472..2497)
                     /label="c-Myc_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2472..2497)
                     /label="C-epitope_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             2473..2505
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2505..2507
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             2518..2535
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(2521..2569)
                     /label="GRo015.3"
                     /note="sequence: gcacagtcgaggctgatcagcgggtttaaactcaatgatgattatgatg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2536..2538
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(2539..2557)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2551..2569)
                     /label="XZp086.2"
                     /note="sequence: gcacagtcgaggctgatca"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2553..2570)
                     /label="pNSK316"
                     /note="sequence: ggcacagtcgaggctgat"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     polyA_signal    2557..2771
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(2558..2575)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2558..2593)
                     /label="NK-triggerEK0300-Sanger-r"
                     /note="sequence: caacagatggctggcaactagaaggcacagtcgagg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2613..2632)
                     /label="XZ41-test-R1"
                     /note="sequence: CACCTTCCAGGGTCAAGGAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2637..2656)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2744..2771)
                     /label="oLSM0086.2"
                     /note="sequence: TCCCCAGCATGCCTGCTATTGTCTTCCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2748..2766
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2751..2768)
                     /label="EKo326.3"
                     /note="sequence: ccagcatgcctgctattg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2751..2771)
                     /label="oLSM0086"
                     /note="sequence: TCCCCAGCATGCCTGCTATTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    2804..2827
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          2828..2846
                     /label="EKo293.3"
                     /note="sequence: cttctgaggcggaaagaac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2906..2921)
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3038..3056
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3110..3157
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(3128..3159)
                     /label="pNSK331"
                     /note="sequence: aggaagttcctatactttctagagaataggaa"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3131..3158)
                     /label="EKo299.3"
                     /note="sequence: ggaagttcctatactttctagagaatag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            3165..4185
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3393..3414
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4113..4131
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4316..4388)
                     /label="NK174-tile1-r"
                     /note="sequence: AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    4317..4447
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(4330..4355)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4341..4398)
                     /label="NK174-tile2-r"
                     /note="sequence: GCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4354..4373)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4487..4503)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4501..4520)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4549..4571)
                     /label="An-o17 U6_3MS2_Cre_seq"
                     /note="sequence: ccaggctttacactttatgcttc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4779..4798
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4830..4854
                     /label="oLSM-pUC_ori_F"
                     /note="sequence: atgtgagcaaaaggccagcaaaagg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4830..4854)
                     /label="oLSM-pUC_ori_R"
                     /note="sequence: ccttttgctggccttttgctcacat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      4830..5503
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(4950..4969)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(5648..6508)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             complement(5648..6508)
                     /label="Translation 6508-5648"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer          5768..5787
                     /label="pNSK334"
                     /note="sequence: cagtgctgcaatgataccgc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(5773..5791)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(6481..6506)
                     /label="oLSM0054"
                     /note="sequence: gagtattcaacatttccgtgtcgccc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          6481..6512
                     /label="oLSM0053"
                     /note="sequence: gggcgacacggaaatgttgaatactcatactc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6509..6607)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          6603..6619
                     /label="AmpR_seq_F"
                     /note="sequence: ggggttccgcgcacatt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
       61 ctacaacaag gcaaggcttg accgacaatt gccgataaaa taaaagattt tatttagtct
      121 ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta gctgcagtaa
      181 cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
      241 gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg agcagtttcg
      301 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
      361 gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
      421 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
      481 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
      541 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgggaagct tcgccaccAT
      601 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
      661 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
      721 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
      781 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
      841 CGCCGACATT CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
      901 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
      961 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     1021 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     1081 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     1141 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
     1201 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     1261 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAGCg acgcgttgGA
     1321 AGCCAGACAC AAACAGAAAA TTGTGGCTCC GGTGAAACAG ACTTTGAATT TTGACCTTCT
     1381 GAAGCTGGCG GGAGACGTCG AGTCCAACCC CGGTCCCggt accggatccA CCAAGTCGAC
     1441 ACGGATGAGT GTTTCCTTCT ATCCGCGTCA TTGTATGCGA CGGCCATGGA CTGGTTCATA
     1501 GCCGTCTGTT TgGGCCTTAC GTACCGATAG ACCACTGGTG CAAATAGAGA GCTATaccgg
     1561 tactagtGAG GCACGGCATA AGCAAAAGAT CGTAGCCCCA GTAAAGCAAA CACTCAACTT
     1621 CGATCTACTC AAACTCGCAG GTGATGTGGA ATCTAATCCA GGACCCTCGA GTGTCTCAAA
     1681 GGGCGAGGAA CTTTTTACGG GCGTTGTTCC GATCCTCGTC GAATTGGACG GGGACGTTAA
     1741 TGGGCATAAA TTCTCAGTTA GTGGTGAGGG TGAGGGGGAC GCGACATATG GGAAACTTAC
     1801 GCTGAAGTTC ATATGTACCA CCGGAAAATT GCCAGTGCCG TGGCCTACTC TCGTCACCAC
     1861 CCTGACATAT GGTGTTCAGT GTTTTAGCCG GTATCCAGAC CATATGAAAC AGCACGATTT
     1921 TTTTAAATCC GCTATGCCTG AAGGTTACGT ACAGGAGCGA ACGATATTTT TTAAAGACGA
     1981 CGGAAACTAC AAAACTAGAG CCGAGGTAAA ATTCGAGGGT GACACTCTCG TGAACCGGAT
     2041 CGAACTGAAG GGCATAGATT TTAAGGAGGA TGGCAATATA CTGGGCCACA AGCTCGAATA
     2101 CAACTATAAT TCTCATAATG TCTATATCAT GGCGGACAAA CAGAAAAACG GCATCAAGGT
     2161 AAACTTTAAA ATCCGACACA ATATTGAGGA CGGCTCTGTC CAGCTTGCGG ACCATTACCA
     2221 ACAGAACACG CCGATAGGGG ATGGTCCAGT ACTTCTCCCT GATAATCACT ATTTGTCTAC
     2281 TCAGTCAGCT CTTAGCAAGG ACCCAAACGA AAAACGGGAT CACATGGTCC TTCTTGAGTT
     2341 CGTCACTGCG GCTGGGATAA CACTCGGGAT GGACGAACTG TATAAGtagg ccggagggcc
     2401 cACGTACACC ATCAGGGTAC GTCTCAGACA CCATCAGGGT CTGTCTGGTA CAGCATCAGC
     2461 GTACCATggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat
     2521 catcataatc atcattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2581 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2641 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2701 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2761 catgctgggg atgcggtggg ctctatggag atctatcgat gttctgggga tgcggtgggc
     2821 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2881 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2941 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3001 ggctttcccc gtcaagctct aaatcggggg tccctttagg gttccgattt agtgctttac
     3061 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat
     3121 tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca
     3181 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     3241 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     3301 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     3361 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     3421 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     3481 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     3541 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     3601 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     3661 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     3721 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     3781 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     3841 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     3901 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     3961 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     4021 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     4081 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     4141 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag
     4201 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4261 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     4321 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4381 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4441 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     4501 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4561 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4681 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     5341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     6661 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcc
//