LOCUS       NSK241_SFFV-mCherry-SGc 6779 bp ds-DNA     circular     18-JUN-2024
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     primer          1..21
                     /label="FlpInF1"
                     /note="sequence: agtatctgctccctgcttgtg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          65..82
                     /label="FlpInF"
                     /note="sequence: aacaaggcaaggcttgac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          65..83
                     /label="EF1a_F"
                     /note="sequence: aacaaggcaaggcttgacc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          76..121
                     /label="EKo285.1"
                     /note="sequence: gcttgaccgacaattgccgataaaataaaagattttatttagtctc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(83..114)
                     /label="EKo695.2"
                     /note="sequence: aataaaatcttttattttatcggcaattgtcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        92..585
                     /label="SFFV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          93..167
                     /label="EWo118.11"
                     /note="sequence: cgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(94..171)
                     /label="GRo003.4"
                     /note="sequence: ctagcttgccataacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(97..171)
                     /label="EWo118.12"
                     /note="sequence: ctagcttgccataacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttatttt"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          98..167
                     /label="GRo003.3"
                     /note="sequence: aaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    160..160
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    188..188
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    266..266
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    363..363
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          472..500
                     /label="SSFV-NKseq-f"
                     /note="sequence: ccaatcagcctgcttctcgcttctgttcg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    504..520
                     /label="EKo438.IVT-1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          504..520
                     /label="EK_SFFV_F2"
                     /note="sequence: gcttctgcttcccgagc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          569..610
                     /label="oLSM0127-temp"
                     /note="sequence: agactgagtcgcccgggaagcttcgccaccATGGTGAGCAAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(572..601)
                     /label="EKo271.2"
                     /note="sequence: catggtggcgaagcttcccgggcgactcag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          572..607
                     /label="XZp054.1"
                     /note="sequence: ctgagtcgcccgggaagcttcgccaccATGGTGAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(574..601)
                     /label="GRo0112.2"
                     /note="sequence: catggtggcgaagcttcccgggcgactc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          575..613
                     /label="EKo412.2"
                     /note="sequence: agtcgcccgggaagcttcgccaccATGgtgagcaagggc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          576..601
                     /label="EK_SFFV_F"
                     /note="sequence: gtcgcccgggaagcttcgccaccatg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    586..591
                     /label="hindII site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          586..601
                     /label="EKo271.3"
                     /note="sequence: aagcttcgccaccatg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          587..602
                     /label="EKo309.1"
                     /note="sequence: agcttcgccaccatgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          587..617
                     /label="EKo326.2"
                     /note="sequence: agcttcgccaccATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    591..598
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          593..610
                     /label="YXo023.1"
                     /note="sequence: gccaccatgGTGAGCAAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(593..610)
                     /label="YXo021.2"
                     /note="sequence: CTTGCTCACCATggtggc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(596..611)
                     /label="Cit_1"
                     /note="sequence: ccttgctcaccatGGT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             599..601
                     /label="Translation 599-601"
                     /translation="M"
     primer          599..615
                     /label="XZp042.5"
                     /note="sequence: atggtgagcaagggcga"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          599..616
                     /label="pNSK019"
                     /note="sequence: ATGGTGAGCAAGGGCGAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             602..1309
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff4273"
                     /ApEinfo_fwdcolor="#ff4273"
     CDS             602..1309
                     /label="Translation 602-1309"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKS"
     primer          complement(605..619)
                     /label="Che_5"
                     /note="sequence: ctcctcgcccttgct"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(637..654)
                     /label="che_8"
                     /note="sequence: aagcgcatgaactccttg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(637..654)
                     /label="Che_8"
                     /note="sequence: aagcgcatgaactccttg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          683..700
                     /label="YX023-seq-1"
                     /note="sequence: GGCCACGAGTTCGAGATC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(737..753)
                     /label="EKo230.1"
                     /note="sequence: TTCAGCTTGgcggtctg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          800..818
                     /label="mChe_4"
                     /note="sequence: cctcagttcatgtacggct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(805..822)
                     /label="mChe_1"
                     /note="sequence: ttggagccgtacatgaac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(867..881)
                     /label="mChe_3"
                     /note="sequence: CCTCGGGGAAGGACA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(901..920)
                     /label="pNSK093"
                     /note="sequence: cgccgtcctcgaagttcatc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(954..969)
                     /label="Che_6"
                     /note="sequence: ATGAACTCGCCGTCCT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1026..1047
                     /label="YX025-seq-2"
                     /note="sequence: agaagaccatgggctgggaggc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1159..1180
                     /label="NK-cherry-seq"
                     /note="sequence: GGCCAAGAAGCCCGTGCAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1159..1180
                     /label="NK-cherry-seq"
                     /note="sequence: GGCCAAGAAGCCCGTGCAGCTG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1185..1203)
                     /label="XZp081.3"
                     /note="sequence: ATGTTGACGTTGTAGGCGC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1202..1223
                     /label="YX017-seq-1"
                     /note="sequence: atcaagttggacatcacctccc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1215..1230
                     /label="mChe_2"
                     /note="sequence: TCACCTCCCACAACGA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1270..1284
                     /label="Che_7"
                     /note="sequence: CCGCCACTCCACCGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1284..1299
                     /label="XZp073.1"
                     /note="sequence: GCGGCATGGACGAGCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1286..1306)
                     /label="pNSK091"
                     /note="sequence: cttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1288..1308
                     /label="Che9"
                     /note="sequence: catggacgagctgtacaagAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1288..1309
                     /label="JSo042_sensor_qPCR_F1"
                     /note="sequence: CATGGACGAGCTGTACAAGAGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1288..1327)
                     /label="EKo408.1"
                     /note="sequence: TCTGGCTTCcaacgcgtcGCTCTTGTACAGCTCGTCCATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1290..1309)
                     /label="XZp092.2"
                     /note="sequence: GCTCTTGTACAGCTCGTCCA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1310..1318
                     /label="Translation 1310-1318"
                     /translation="DAL"
     primer          1310..1327
                     /label="pNSK124"
                     /note="sequence: gacgcgttgGAAGCCAGA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1312..1391
                     /label="CCo97 F2A-SensorMut-F1"
                     /note="sequence: cgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1313..1330
                     /label="pNSK131"
                     /note="sequence: gcgttgGAAGCCAGACAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1319..1336
                     /label="EKo260.2"
                     /note="sequence: GAAGCCAGACACAAACAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1319..1417
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1319..1417
                     /label="Translation 1319-1417"
                     /translation="EARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP"
     primer          1343..1360
                     /label="Pepper-seq-F"
                     /note="sequence: GTGGCTCCGGTGAAACAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1371..1389
                     /label="JSo044_sensor_qPCR_F2_OLD"
                     /note="sequence: TTGACCTTCTGAAGCTGGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1380..1396)
                     /label="XZ071-test-R"
                     /note="sequence: GTCTCCCGCCAGCTTCA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1402..1417)
                     /label="JMo3.2"
                     /note="sequence: GGGACCGGGGTTGGAC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1403..1417
                     /label="EK-5SGF"
                     /note="sequence: TCCAACCCCGGTCCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1403..1417)
                     /label="Mre_short_R"
                     /note="sequence: GGGACCGGGGTTGGA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1404..1418
                     /label="XZp087.1"
                     /note="sequence: CCAACCCCGGTCCCg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1413..1429
                     /label="XZp082.1"
                     /note="sequence: GTCCCggtaccggatcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1418..1423
                     /label="Translation 1418-1423"
                     /translation="GT"
     CDS             1424..1429
                     /label="Translation 1424-1429"
                     /translation="GS"
     primer          1425..1520
                     /label="pNSK670"
                     /note="sequence: gatccCGAAATCTTCGAGTGTGgAGACgtgctgctctatgccagcatttcTGGCATATGCGACGGCCATGGAtccttatatttaaggaatgtgagc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1429..1524)
                     /label="pNSK671"
                     /note="sequence: aggtgctcacattccttaaatataaggaTCCATGGCCGTCGCATATGCCAgaaatgctggcatagagcagcacGTCTcCACACTCGAAGATTTCGg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1430..1474
                     /label="reporter-left"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1430..1615
                     /label="Translation 1430-1615"
                     /translation="RNLRVWRRAALCQHFWHMRRPWILIFKECEHLPSFWFCLKPS*PSVMHIIASMIATHVPWNS"
     misc_feature    1447..1447
                     /label="A>G"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1475..1475
                     /label="bulge"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1475..1570
                     /label="GABRA3-derived stemloop"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1482..1482
                     /label="bulge"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1491..1491
                     /label="C opposite A"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1497..1551)
                     /label="SMN2 ex7 loop"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(1503..1503)
                     /label="A insert"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1521..1616
                     /label="pNSK672"
                     /note="sequence: accttccttctttttgGttttgtctaaaaccTTCATAGCCGTCTGTTATGcatataatagccagtatgatagccactcatgtaccatgGaattCaa"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1525..1620)
                     /label="pNSK673"
                     /note="sequence: ccggttGaattCcatggtacatgagtggctatcatactggctattatatgCATAACAGACGGCTATGAAggttttagacaaaaCcaaaaagaagga"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1537..1537
                     /label="A>G"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1557..1557
                     /label="editing target"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1564..1564
                     /label="bulge"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1571..1615
                     /label="reporter-left"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1578..1622
                     /label="rec10-rev-f2"
                     /note="sequence: cacaaCAGAAACGTCACGTCTGGCGacatgaggattacccatgta"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1609..1609
                     /label="A>G"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1614..1614
                     /label="A>C"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             1616..1621
                     /label="Translation 1616-1621"
                     /translation="TG"
     primer          complement(1616..1634)
                     /label="EKo706.3"
                     /note="sequence: GTGCCTCactagtaccggt"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1622..1627
                     /label="Translation 1622-1627"
                     /translation="TS"
     primer          1628..1643
                     /label="EKo242.3"
                     /note="sequence: GAGGCACGGCATAAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1628..1643)
                     /label="EK-3SGR"
                     /note="sequence: GCTTATGCCGTGCCTC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1628..1726
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1628..1726
                     /label="Translation 1628-1726"
                     /translation="EARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP"
     primer          complement(1658..1681)
                     /label="JSo045_sensor_qPCR_R2"
                     /note="sequence: GAAGTTGAGTGTTTGCTTTACTGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1701..1731)
                     /label="oLSM0052"
                     /note="sequence: CTCGAGGGTCCTGGATTAGATTCCACATCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1703..1726)
                     /label="pNSK126"
                     /note="sequence: GGGTCCTGGATTAGATTCCACATC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1706..1726)
                     /label="EKo222.1"
                     /note="sequence: AGGTCCTGGATTAGATTCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1706..1726)
                     /label="EKo387.3"
                     /note="sequence: GGGTCCTGGATTAGATTCCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1707..1751
                     /label="RVJK006_2_F"
                     /note="sequence: TGGAATCTAATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1715..1751
                     /label="EKo409.1"
                     /note="sequence: AATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1727..1732
                     /label="Translation 1727-1732"
                     /translation="SS"
     primer          1733..1751
                     /label="EKo147.1"
                     /note="sequence: GTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1733..1760
                     /label="oLSM0117"
                     /note="sequence: GTCTCAAAGGGCGAGGAACTTTTTACGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1733..2446
                     /label="GFP"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1733..2446
                     /label="Translation 1733-2446"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer          complement(1766..1786)
                     /label="GFP5"
                     /note="sequence: CAATTCGACGAGGATCGGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1770..1789)
                     /label="JSo043_sensor_qPCR_R1"
                     /note="sequence: GTCCAATTCGACGAGGATCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1795..1824)
                     /label="NK-gfp-seq-r"
                     /note="sequence: CCACTAACTGAGAATTTATGCCCATTAACG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1881..1899
                     /label="pNSK084"
                     /note="sequence: CCGGAAAATTGCCAGTGCC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1885..1900
                     /label="EWo208.1"
                     /note="sequence: AAAATTGCCAGTGCCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1898..1914)
                     /label="CCp019-repGFPrev"
                     /note="sequence: ACGAGAGTAGGCCACGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1989..2005)
                     /label="EKo224.2"
                     /note="sequence: ACCTTCAGGCATAGCGG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2097..2117
                     /label="pPYK028"
                     /note="sequence: GGATCGAACTGAAGGGCATAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2101..2126
                     /label="YX017-seq-2"
                     /note="sequence: CGAACTGAAGGGCATAGATTTTAAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2374..2393
                     /label="GFP6"
                     /note="sequence: ACGGGATCACATGGTCCTTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2415..2449)
                     /label="CCp024-GFP-r"
                     /note="sequence: CTACTTATACAGTTCGTCCATCCCGAGTGTTATCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2425..2446
                     /label="EKoCC34.2"
                     /note="sequence: CGGGATGGACGAACTGTATAAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2425..2446)
                     /label="EKo460.1"
                     /note="sequence: CTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2425..2449)
                     /label="pNSK025"
                     /note="sequence: ctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2425..2449)
                     /label="JSo033_sensorGFP_R"
                     /note="sequence: ctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2425..2460)
                     /label="YXo007.2"
                     /note="sequence: ggccctccggcctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2447..2449
                     /label="Translation 2447-2449"
                     /translation="*"
     misc_feature    2456..2461
                     /label="apaI site"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(2457..2492)
                     /label="EKo438.3"
                     /note="sequence: GGTGTCTGAGACGTACCCTGATGGTGTACGTgggcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2461..2496
                     /label="SB011.1"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2461..2497
                     /label="EKo438.1"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          2461..2528
                     /label="Rec14-US-3xMS2nbt-f"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2462..2527
                     /label="3xMS2 SGR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2471..2474
                     /label="loop MS2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2493..2496
                     /label="loop MS2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2493..2527)
                     /label="EKo438.4"
                     /note="sequence: ATGGTACGCTGATGCTGTACCAGACAGACCCTGAT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2497..2528
                     /label="SB011.2"
                     /note="sequence: GGGTCTGTCTGGTACAGCATCAGCGTACCATg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2498..2531
                     /label="EKo438.2"
                     /note="sequence: GGTCTGTCTGGTACAGCATCAGCGTACCATggcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2515..2518
                     /label="loop MS2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2528..2531
                     /label="overhang"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2528..2548)
                     /label="RXR_CD8_R"
                     /note="sequence: agatgagtttttgTTCgggcc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2528..2549)
                     /label="pNK018"
                     /note="sequence: gagatgagtttttgttcGGGCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2532..2557)
                     /label="c-Myc_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2532..2557)
                     /label="C-epitope_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             2533..2565
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2565..2567
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             2578..2595
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(2581..2629)
                     /label="GRo015.3"
                     /note="sequence: gcacagtcgaggctgatcagcgggtttaaactcaatgatgattatgatg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2596..2598
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(2599..2617)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2611..2629)
                     /label="XZp086.2"
                     /note="sequence: gcacagtcgaggctgatca"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2613..2630)
                     /label="pNSK316"
                     /note="sequence: ggcacagtcgaggctgat"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     polyA_signal    2617..2831
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(2618..2635)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2618..2653)
                     /label="NK-triggerEK0300-Sanger-r"
                     /note="sequence: caacagatggctggcaactagaaggcacagtcgagg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2673..2692)
                     /label="XZ41-test-R1"
                     /note="sequence: CACCTTCCAGGGTCAAGGAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2697..2716)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2804..2831)
                     /label="oLSM0086.2"
                     /note="sequence: TCCCCAGCATGCCTGCTATTGTCTTCCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2808..2826
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2811..2828)
                     /label="EKo326.3"
                     /note="sequence: ccagcatgcctgctattg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2811..2831)
                     /label="oLSM0086"
                     /note="sequence: TCCCCAGCATGCCTGCTATTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    2864..2887
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          2888..2906
                     /label="EKo293.3"
                     /note="sequence: cttctgaggcggaaagaac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2966..2981)
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3098..3116
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3170..3217
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(3188..3219)
                     /label="pNSK331"
                     /note="sequence: aggaagttcctatactttctagagaataggaa"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3191..3218)
                     /label="EKo299.3"
                     /note="sequence: ggaagttcctatactttctagagaatag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            3225..4245
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3453..3474
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4173..4191
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4376..4448)
                     /label="NK174-tile1-r"
                     /note="sequence: AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    4377..4507
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(4390..4415)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4401..4458)
                     /label="NK174-tile2-r"
                     /note="sequence: GCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4414..4433)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4547..4563)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4561..4580)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4609..4631)
                     /label="An-o17 U6_3MS2_Cre_seq"
                     /note="sequence: ccaggctttacactttatgcttc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4839..4858
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4890..4914
                     /label="oLSM-pUC_ori_F"
                     /note="sequence: atgtgagcaaaaggccagcaaaagg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4890..4914)
                     /label="oLSM-pUC_ori_R"
                     /note="sequence: ccttttgctggccttttgctcacat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      4890..5563
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(5010..5029)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(5708..6568)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             complement(5708..6568)
                     /label="Translation 6568-5708"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer          5828..5847
                     /label="pNSK334"
                     /note="sequence: cagtgctgcaatgataccgc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(5833..5851)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(6541..6566)
                     /label="oLSM0054"
                     /note="sequence: gagtattcaacatttccgtgtcgccc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          6541..6572
                     /label="oLSM0053"
                     /note="sequence: gggcgacacggaaatgttgaatactcatactc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6569..6667)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          6663..6679
                     /label="AmpR_seq_F"
                     /note="sequence: ggggttccgcgcacatt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
       61 ctacaacaag gcaaggcttg accgacaatt gccgataaaa taaaagattt tatttagtct
      121 ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta gctgcagtaa
      181 cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
      241 gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg agcagtttcg
      301 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
      361 gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
      421 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
      481 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
      541 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgggaagct tcgccaccAT
      601 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
      661 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
      721 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
      781 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
      841 CGCCGACATT CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
      901 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
      961 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     1021 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     1081 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     1141 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
     1201 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     1261 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAGCg acgcgttgGA
     1321 AGCCAGACAC AAACAGAAAA TTGTGGCTCC GGTGAAACAG ACTTTGAATT TTGACCTTCT
     1381 GAAGCTGGCG GGAGACGTCG AGTCCAACCC CGGTCCCggt accggatccC GAAATCTTCG
     1441 AGTGTGgAGA Cgtgctgctc tatgccagca tttcTGGCAT ATGCGACGGC CATGGAtcct
     1501 tatatttaag gaatgtgagc accttccttc tttttgGttt tgtctaaaac cTTCATAGCC
     1561 GTCTGTTATG catataatag ccagtatgat agccactcat gtaccatgGa attCaaccgg
     1621 tactagtGAG GCACGGCATA AGCAAAAGAT CGTAGCCCCA GTAAAGCAAA CACTCAACTT
     1681 CGATCTACTC AAACTCGCAG GTGATGTGGA ATCTAATCCA GGACCCTCGA GTGTCTCAAA
     1741 GGGCGAGGAA CTTTTTACGG GCGTTGTTCC GATCCTCGTC GAATTGGACG GGGACGTTAA
     1801 TGGGCATAAA TTCTCAGTTA GTGGTGAGGG TGAGGGGGAC GCGACATATG GGAAACTTAC
     1861 GCTGAAGTTC ATATGTACCA CCGGAAAATT GCCAGTGCCG TGGCCTACTC TCGTCACCAC
     1921 CCTGACATAT GGTGTTCAGT GTTTTAGCCG GTATCCAGAC CATATGAAAC AGCACGATTT
     1981 TTTTAAATCC GCTATGCCTG AAGGTTACGT ACAGGAGCGA ACGATATTTT TTAAAGACGA
     2041 CGGAAACTAC AAAACTAGAG CCGAGGTAAA ATTCGAGGGT GACACTCTCG TGAACCGGAT
     2101 CGAACTGAAG GGCATAGATT TTAAGGAGGA TGGCAATATA CTGGGCCACA AGCTCGAATA
     2161 CAACTATAAT TCTCATAATG TCTATATCAT GGCGGACAAA CAGAAAAACG GCATCAAGGT
     2221 AAACTTTAAA ATCCGACACA ATATTGAGGA CGGCTCTGTC CAGCTTGCGG ACCATTACCA
     2281 ACAGAACACG CCGATAGGGG ATGGTCCAGT ACTTCTCCCT GATAATCACT ATTTGTCTAC
     2341 TCAGTCAGCT CTTAGCAAGG ACCCAAACGA AAAACGGGAT CACATGGTCC TTCTTGAGTT
     2401 CGTCACTGCG GCTGGGATAA CACTCGGGAT GGACGAACTG TATAAGtagg ccggagggcc
     2461 cACGTACACC ATCAGGGTAC GTCTCAGACA CCATCAGGGT CTGTCTGGTA CAGCATCAGC
     2521 GTACCATggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat
     2581 catcataatc atcattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2821 catgctgggg atgcggtggg ctctatggag atctatcgat gttctgggga tgcggtgggc
     2881 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3061 ggctttcccc gtcaagctct aaatcggggg tccctttagg gttccgattt agtgctttac
     3121 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat
     3181 tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca
     3241 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     3301 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     3361 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     3421 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     3481 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     3541 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     3601 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     3661 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     3721 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     3781 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     3841 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     3901 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     3961 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     4021 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     4081 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     4141 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     4201 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag
     4261 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4321 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     4381 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4441 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4501 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     4561 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4621 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4681 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4741 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4801 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     5401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5461 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5521 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5581 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5641 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5701 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5761 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5821 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5881 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5941 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6001 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6061 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6121 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6181 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6241 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6301 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6361 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6421 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6481 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6541 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6601 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6661 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     6721 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcc
//