LOCUS       NSK266_SFFV-mCherry-SGc 6683 bp ds-DNA     circular     28-AUG-2024
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     primer          1..21
                     /label="FlpInF1"
                     /note="sequence: agtatctgctccctgcttgtg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          65..82
                     /label="FlpInF"
                     /note="sequence: aacaaggcaaggcttgac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          65..83
                     /label="EF1a_F"
                     /note="sequence: aacaaggcaaggcttgacc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          76..121
                     /label="EKo285.1"
                     /note="sequence: gcttgaccgacaattgccgataaaataaaagattttatttagtctc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(83..114)
                     /label="EKo695.2"
                     /note="sequence: aataaaatcttttattttatcggcaattgtcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        92..585
                     /label="SFFV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          93..167
                     /label="EWo118.11"
                     /note="sequence: cgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaag"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(94..171)
                     /label="GRo003.4"
                     /note="sequence: ctagcttgccataacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(97..171)
                     /label="EWo118.12"
                     /note="sequence: ctagcttgccataacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttatttt"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          98..167
                     /label="GRo003.3"
                     /note="sequence: aaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggttatggcaag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    160..160
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    188..188
                     /label="missing in pSLQ2349"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    266..266
                     /label="A->T from pSLQ2349"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    363..363
                     /label="G->A from pSLQ2349"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          472..500
                     /label="SSFV-NKseq-f"
                     /note="sequence: ccaatcagcctgcttctcgcttctgttcg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    504..520
                     /label="EKo438.IVT-1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          504..520
                     /label="EK_SFFV_F2"
                     /note="sequence: gcttctgcttcccgagc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          569..610
                     /label="oLSM0127-temp"
                     /note="sequence: agactgagtcgcccgggaagcttcgccaccATGGTGAGCAAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(572..601)
                     /label="EKo271.2"
                     /note="sequence: catggtggcgaagcttcccgggcgactcag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          572..607
                     /label="XZp054.1"
                     /note="sequence: ctgagtcgcccgggaagcttcgccaccATGGTGAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(574..601)
                     /label="GRo0112.2"
                     /note="sequence: catggtggcgaagcttcccgggcgactc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          575..613
                     /label="EKo412.2"
                     /note="sequence: agtcgcccgggaagcttcgccaccATGgtgagcaagggc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          576..601
                     /label="EK_SFFV_F"
                     /note="sequence: gtcgcccgggaagcttcgccaccatg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          586..601
                     /label="EKo271.3"
                     /note="sequence: aagcttcgccaccatg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          587..602
                     /label="EKo309.1"
                     /note="sequence: agcttcgccaccatgg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          587..617
                     /label="EKo326.2"
                     /note="sequence: agcttcgccaccATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    591..598
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          593..610
                     /label="YXo023.1"
                     /note="sequence: gccaccatgGTGAGCAAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(593..610)
                     /label="YXo021.2"
                     /note="sequence: CTTGCTCACCATggtggc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(596..611)
                     /label="Cit_1"
                     /note="sequence: ccttgctcaccatGGT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             599..601
                     /label="Translation 599-601"
                     /translation="M"
     primer          599..615
                     /label="XZp042.5"
                     /note="sequence: atggtgagcaagggcga"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          599..616
                     /label="pNSK019"
                     /note="sequence: ATGGTGAGCAAGGGCGAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             602..1309
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff4273"
                     /ApEinfo_fwdcolor="#ff4273"
     CDS             602..1309
                     /label="Translation 602-1309"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKS"
     primer          complement(605..619)
                     /label="Che_5"
                     /note="sequence: ctcctcgcccttgct"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(637..654)
                     /label="Che_8"
                     /note="sequence: aagcgcatgaactccttg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(637..654)
                     /label="che_8"
                     /note="sequence: aagcgcatgaactccttg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          683..700
                     /label="YX023-seq-1"
                     /note="sequence: GGCCACGAGTTCGAGATC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(737..753)
                     /label="EKo230.1"
                     /note="sequence: TTCAGCTTGgcggtctg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          800..818
                     /label="mChe_4"
                     /note="sequence: cctcagttcatgtacggct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(805..822)
                     /label="mChe_1"
                     /note="sequence: ttggagccgtacatgaac"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(867..881)
                     /label="mChe_3"
                     /note="sequence: CCTCGGGGAAGGACA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(901..920)
                     /label="pNSK093"
                     /note="sequence: cgccgtcctcgaagttcatc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(954..969)
                     /label="Che_6"
                     /note="sequence: ATGAACTCGCCGTCCT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1026..1047
                     /label="YX025-seq-2"
                     /note="sequence: agaagaccatgggctgggaggc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1159..1180
                     /label="NK-cherry-seq"
                     /note="sequence: GGCCAAGAAGCCCGTGCAGCTG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1159..1180
                     /label="NK-cherry-seq"
                     /note="sequence: GGCCAAGAAGCCCGTGCAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1185..1203)
                     /label="XZp081.3"
                     /note="sequence: ATGTTGACGTTGTAGGCGC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1202..1223
                     /label="YX017-seq-1"
                     /note="sequence: atcaagttggacatcacctccc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1215..1230
                     /label="mChe_2"
                     /note="sequence: TCACCTCCCACAACGA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1270..1284
                     /label="Che_7"
                     /note="sequence: CCGCCACTCCACCGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1284..1299
                     /label="XZp073.1"
                     /note="sequence: GCGGCATGGACGAGCT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1286..1306)
                     /label="pNSK091"
                     /note="sequence: cttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1288..1308
                     /label="Che9"
                     /note="sequence: catggacgagctgtacaagAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1288..1309
                     /label="JSo042_sensor_qPCR_F1"
                     /note="sequence: CATGGACGAGCTGTACAAGAGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1288..1327)
                     /label="EKo408.1"
                     /note="sequence: TCTGGCTTCcaacgcgtcGCTCTTGTACAGCTCGTCCATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1290..1309)
                     /label="XZp092.2"
                     /note="sequence: GCTCTTGTACAGCTCGTCCA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1310..1318
                     /label="Translation 1310-1318"
                     /translation="DAL"
     primer          1310..1327
                     /label="pNSK124"
                     /note="sequence: gacgcgttgGAAGCCAGA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1312..1391
                     /label="CCo97 F2A-SensorMut-F1"
                     /note="sequence: cgcgttgGAAGCCAGACACAAACAGAAAATTGTGGCTCCGGTGAAACAGACTTTGAATTTTGACCTTCTGAAGCTGGCGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1313..1330
                     /label="pNSK131"
                     /note="sequence: gcgttgGAAGCCAGACAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1319..1336
                     /label="EKo260.2"
                     /note="sequence: GAAGCCAGACACAAACAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1319..1417
                     /label="5' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             1319..1417
                     /label="Translation 1319-1417"
                     /translation="EARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP"
     primer          1343..1360
                     /label="Pepper-seq-F"
                     /note="sequence: GTGGCTCCGGTGAAACAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1371..1389
                     /label="JSo044_sensor_qPCR_F2_OLD"
                     /note="sequence: TTGACCTTCTGAAGCTGGC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1380..1396)
                     /label="XZ071-test-R"
                     /note="sequence: GTCTCCCGCCAGCTTCA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1402..1417)
                     /label="JMo3.2"
                     /note="sequence: GGGACCGGGGTTGGAC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1403..1417
                     /label="EK-5SGF"
                     /note="sequence: TCCAACCCCGGTCCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1403..1417)
                     /label="Mre_short_R"
                     /note="sequence: GGGACCGGGGTTGGA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1404..1418
                     /label="XZp087.1"
                     /note="sequence: CCAACCCCGGTCCCg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1413..1429
                     /label="XZp082.1"
                     /note="sequence: GTCCCggtaccggatcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1418..1423
                     /label="Translation 1418-1423"
                     /translation="GT"
     misc_feature    1424..1429
                     /label="bamhi site"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             1424..1429
                     /label="Translation 1424-1429"
                     /translation="GS"
     primer          1425..1520
                     /label="pNSK750"
                     /note="sequence: gatccGGACCACCAGCTGGTACTTGgCCGAAGACGGCAGAAAGCAGAGTCTaGGAAGACAACCACAGACACCGTCCTCACCACCCGGGACTTGTAa"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1429..1524)
                     /label="pNSK751"
                     /note="sequence: ccggtTACAAGTCCCGGGTGGTGAGGACGGTGTCTGTGGTTGTCTTCCtAGACTCTGCTTTCTGCCGTCTTCGGcCAAGTACCAGCTGGTGGTCCg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1430..1519
                     /label="EBER1.linear.2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1430..1519
                     /label="Translation 1430-1519"
                     /translation="GPPAGTWPKTAESRV*EDNHRHRPHHPGLV"
     misc_feature    1448..1450
                     /label="a>g"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1520..1525
                     /label="bshti site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1520..1525
                     /label="Translation 1520-1525"
                     /translation="TG"
     primer          complement(1520..1538)
                     /label="EKo706.3"
                     /note="sequence: GTGCCTCactagtaccggt"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             1526..1531
                     /label="Translation 1526-1531"
                     /translation="TS"
     primer          1532..1547
                     /label="EKo242.3"
                     /note="sequence: GAGGCACGGCATAAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1532..1547)
                     /label="EK-3SGR"
                     /note="sequence: GCTTATGCCGTGCCTC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1532..1630
                     /label="3' StopGo (2A; Loughran 2017)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1532..1630
                     /label="Translation 1532-1630"
                     /translation="EARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP"
     primer          complement(1562..1585)
                     /label="JSo045_sensor_qPCR_R2"
                     /note="sequence: GAAGTTGAGTGTTTGCTTTACTGG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1605..1635)
                     /label="oLSM0052"
                     /note="sequence: CTCGAGGGTCCTGGATTAGATTCCACATCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1607..1630)
                     /label="pNSK126"
                     /note="sequence: GGGTCCTGGATTAGATTCCACATC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1610..1630)
                     /label="EKo387.3"
                     /note="sequence: GGGTCCTGGATTAGATTCCAC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1610..1630)
                     /label="EKo222.1"
                     /note="sequence: AGGTCCTGGATTAGATTCCAC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1611..1655
                     /label="RVJK006_2_F"
                     /note="sequence: TGGAATCTAATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1619..1655
                     /label="EKo409.1"
                     /note="sequence: AATCCAGGACCCTCGAGTGTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1631..1636
                     /label="Translation 1631-1636"
                     /translation="SS"
     primer          1637..1655
                     /label="EKo147.1"
                     /note="sequence: GTCTCAAAGGGCGAGGAAC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1637..1664
                     /label="oLSM0117"
                     /note="sequence: GTCTCAAAGGGCGAGGAACTTTTTACGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1637..2350
                     /label="GFP"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             1637..2350
                     /label="Translation 1637-2350"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer          complement(1670..1690)
                     /label="GFP5"
                     /note="sequence: CAATTCGACGAGGATCGGAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1674..1693)
                     /label="JSo043_sensor_qPCR_R1"
                     /note="sequence: GTCCAATTCGACGAGGATCG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(1699..1728)
                     /label="NK-gfp-seq-r"
                     /note="sequence: CCACTAACTGAGAATTTATGCCCATTAACG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1785..1803
                     /label="pNSK084"
                     /note="sequence: CCGGAAAATTGCCAGTGCC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1789..1804
                     /label="EWo208.1"
                     /note="sequence: AAAATTGCCAGTGCCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1802..1818)
                     /label="CCp019-repGFPrev"
                     /note="sequence: ACGAGAGTAGGCCACGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1893..1909)
                     /label="EKo224.2"
                     /note="sequence: ACCTTCAGGCATAGCGG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2001..2021
                     /label="pPYK028"
                     /note="sequence: GGATCGAACTGAAGGGCATAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2005..2030
                     /label="YX017-seq-2"
                     /note="sequence: CGAACTGAAGGGCATAGATTTTAAGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2278..2297
                     /label="GFP6"
                     /note="sequence: ACGGGATCACATGGTCCTTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2319..2353)
                     /label="CCp024-GFP-r"
                     /note="sequence: CTACTTATACAGTTCGTCCATCCCGAGTGTTATCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2329..2350
                     /label="EKoCC34.2"
                     /note="sequence: CGGGATGGACGAACTGTATAAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2329..2350)
                     /label="EKo460.1"
                     /note="sequence: CTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2329..2353)
                     /label="pNSK025"
                     /note="sequence: ctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2329..2353)
                     /label="JSo033_sensorGFP_R"
                     /note="sequence: ctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2329..2364)
                     /label="YXo007.2"
                     /note="sequence: ggccctccggcctaCTTATACAGTTCGTCCATCCCG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2351..2353
                     /label="Translation 2351-2353"
                     /translation="*"
     primer          complement(2361..2396)
                     /label="EKo438.3"
                     /note="sequence: GGTGTCTGAGACGTACCCTGATGGTGTACGTgggcc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2365..2400
                     /label="SB011.1"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCA"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2365..2401
                     /label="EKo438.1"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          2365..2432
                     /label="Rec14-US-3xMS2nbt-f"
                     /note="sequence: cACGTACACCATCAGGGTACGTCTCAGACACCATCAGGGTCTGTCTGGTACAGCATCAGCGTACCATg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2366..2431
                     /label="3xMS2 SGR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2375..2378
                     /label="loop MS2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2397..2400
                     /label="loop MS2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2397..2431)
                     /label="EKo438.4"
                     /note="sequence: ATGGTACGCTGATGCTGTACCAGACAGACCCTGAT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2401..2432
                     /label="SB011.2"
                     /note="sequence: GGGTCTGTCTGGTACAGCATCAGCGTACCATg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2402..2435
                     /label="EKo438.2"
                     /note="sequence: GGTCTGTCTGGTACAGCATCAGCGTACCATggcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2419..2422
                     /label="loop MS2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2432..2435
                     /label="overhang"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2432..2452)
                     /label="RXR_CD8_R"
                     /note="sequence: agatgagtttttgTTCgggcc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2432..2453)
                     /label="pNK018"
                     /note="sequence: gagatgagtttttgttcGGGCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(2436..2461)
                     /label="c-Myc_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2436..2461)
                     /label="C-epitope_R"
                     /note="sequence: cctcttctgagatgagtttttgTTCg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     CDS             2437..2469
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2469..2471
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             2482..2499
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(2485..2533)
                     /label="GRo015.3"
                     /note="sequence: gcacagtcgaggctgatcagcgggtttaaactcaatgatgattatgatg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2500..2502
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(2503..2521)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2515..2533)
                     /label="XZp086.2"
                     /note="sequence: gcacagtcgaggctgatca"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2517..2534)
                     /label="pNSK316"
                     /note="sequence: ggcacagtcgaggctgat"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     polyA_signal    2521..2735
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(2522..2539)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2522..2557)
                     /label="NK-triggerEK0300-Sanger-r"
                     /note="sequence: caacagatggctggcaactagaaggcacagtcgagg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2577..2596)
                     /label="XZ41-test-R1"
                     /note="sequence: CACCTTCCAGGGTCAAGGAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2601..2620)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2708..2735)
                     /label="oLSM0086.2"
                     /note="sequence: TCCCCAGCATGCCTGCTATTGTCTTCCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2712..2730
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2715..2732)
                     /label="EKo326.3"
                     /note="sequence: ccagcatgcctgctattg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2715..2735)
                     /label="oLSM0086"
                     /note="sequence: TCCCCAGCATGCCTGCTATTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     polyA_signal    2768..2791
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          2792..2810
                     /label="EKo293.3"
                     /note="sequence: cttctgaggcggaaagaac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2870..2885)
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3002..3020
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3074..3121
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(3092..3123)
                     /label="pNSK331"
                     /note="sequence: aggaagttcctatactttctagagaataggaa"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3095..3122)
                     /label="EKo299.3"
                     /note="sequence: ggaagttcctatactttctagagaatag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            3129..4149
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3357..3378
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4077..4095
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(4280..4352)
                     /label="NK174-tile1-r"
                     /note="sequence: AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    4281..4411
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(4294..4319)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(4305..4362)
                     /label="NK174-tile2-r"
                     /note="sequence: GCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4318..4337)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4451..4467)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4465..4484)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4513..4535)
                     /label="An-o17 U6_3MS2_Cre_seq"
                     /note="sequence: ccaggctttacactttatgcttc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4743..4762
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4794..4818
                     /label="oLSM-pUC_ori_F"
                     /note="sequence: atgtgagcaaaaggccagcaaaagg"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4794..4818)
                     /label="oLSM-pUC_ori_R"
                     /note="sequence: ccttttgctggccttttgctcacat"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      4794..5467
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(4914..4933)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(5612..6472)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             complement(5612..6472)
                     /label="Translation 6472-5612"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer          5732..5751
                     /label="pNSK334"
                     /note="sequence: cagtgctgcaatgataccgc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(5737..5755)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(6445..6470)
                     /label="oLSM0054"
                     /note="sequence: gagtattcaacatttccgtgtcgccc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          6445..6476
                     /label="oLSM0053"
                     /note="sequence: gggcgacacggaaatgttgaatactcatactc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6473..6571)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          6567..6583
                     /label="AmpR_seq_F"
                     /note="sequence: ggggttccgcgcacatt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
       61 ctacaacaag gcaaggcttg accgacaatt gccgataaaa taaaagattt tatttagtct
      121 ccagaaaaag gggggaatga aagaccccac ctgtaggtta tggcaagcta gctgcagtaa
      181 cgccattatt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
      241 gcgggtacat gaaaatagct aacgtagggc caaacaggat atctgcggtg agcagtttcg
      301 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
      361 gagcagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
      421 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
      481 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
      541 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgggaagct tcgccaccAT
      601 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
      661 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
      721 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
      781 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
      841 CGCCGACATT CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
      901 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
      961 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
     1021 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
     1081 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
     1141 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
     1201 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
     1261 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAGCg acgcgttgGA
     1321 AGCCAGACAC AAACAGAAAA TTGTGGCTCC GGTGAAACAG ACTTTGAATT TTGACCTTCT
     1381 GAAGCTGGCG GGAGACGTCG AGTCCAACCC CGGTCCCggt accggatccG GACCACCAGC
     1441 TGGTACTTGg CCGAAGACGG CAGAAAGCAG AGTCTaGGAA GACAACCACA GACACCGTCC
     1501 TCACCACCCG GGACTTGTAa ccggtactag tGAGGCACGG CATAAGCAAA AGATCGTAGC
     1561 CCCAGTAAAG CAAACACTCA ACTTCGATCT ACTCAAACTC GCAGGTGATG TGGAATCTAA
     1621 TCCAGGACCC TCGAGTGTCT CAAAGGGCGA GGAACTTTTT ACGGGCGTTG TTCCGATCCT
     1681 CGTCGAATTG GACGGGGACG TTAATGGGCA TAAATTCTCA GTTAGTGGTG AGGGTGAGGG
     1741 GGACGCGACA TATGGGAAAC TTACGCTGAA GTTCATATGT ACCACCGGAA AATTGCCAGT
     1801 GCCGTGGCCT ACTCTCGTCA CCACCCTGAC ATATGGTGTT CAGTGTTTTA GCCGGTATCC
     1861 AGACCATATG AAACAGCACG ATTTTTTTAA ATCCGCTATG CCTGAAGGTT ACGTACAGGA
     1921 GCGAACGATA TTTTTTAAAG ACGACGGAAA CTACAAAACT AGAGCCGAGG TAAAATTCGA
     1981 GGGTGACACT CTCGTGAACC GGATCGAACT GAAGGGCATA GATTTTAAGG AGGATGGCAA
     2041 TATACTGGGC CACAAGCTCG AATACAACTA TAATTCTCAT AATGTCTATA TCATGGCGGA
     2101 CAAACAGAAA AACGGCATCA AGGTAAACTT TAAAATCCGA CACAATATTG AGGACGGCTC
     2161 TGTCCAGCTT GCGGACCATT ACCAACAGAA CACGCCGATA GGGGATGGTC CAGTACTTCT
     2221 CCCTGATAAT CACTATTTGT CTACTCAGTC AGCTCTTAGC AAGGACCCAA ACGAAAAACG
     2281 GGATCACATG GTCCTTCTTG AGTTCGTCAC TGCGGCTGGG ATAACACTCG GGATGGACGA
     2341 ACTGTATAAG taggccggag ggcccACGTA CACCATCAGG GTACGTCTCA GACACCATCA
     2401 GGGTCTGTCT GGTACAGCAT CAGCGTACCA Tggcccgaac aaaaactcat ctcagaagag
     2461 gatctgaata gcgccgtcga ccatcatcat aatcatcatt gagtttaaac ccgctgatca
     2521 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     2581 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     2641 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     2701 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggagatctat
     2761 cgatgttctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     2821 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2881 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2941 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggtccctt
     3001 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3061 gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
     3121 ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     3181 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     3241 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     3301 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     3361 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     3421 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     3481 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     3541 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     3601 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     3661 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     3721 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     3781 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     3841 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     3901 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     3961 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     4021 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     4081 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     4141 caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4201 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4261 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     4321 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4381 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     4441 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4501 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4561 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4621 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4681 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4741 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5041 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5281 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5461 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5521 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6541 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6601 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     6661 ctctgatgcc gcatagttaa gcc
//