LOCUS       LvPolyubiquitinC-Grx1-roGFP2-lox-minos        7768 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  d3-grx-lvpoly_01.
ACCESSION   d3-grx-lvpoly_01
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     misc_feature_   complement(1..589)
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="H2B_GFP_mAbbsynth in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:58-epr10bc"">H2B_GFP_mAbbsynth in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:5o-epr10cb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a href=""urn:local:.:w-g2s1x8b"">mGreen
                     Lantern</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:h-hh7co6e"">Lifeact-mCherry in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a href=""urn:local:.:1j-he1rucf"">Minos
                     Lox Lvpolyubi LCK mNeongreen</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:n-eurzkb4"">PictusNanos 5' and 3' UTR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:o-hfwuxxr"">EMTB-mCherry</a>)"
                     /Alternative_Match="99.83%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:g-hgwqs99"">pCS2FA-transposase</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="colE1_high_copy"
     primer_bind     490..509
                     /Tm=56
                     /Sequence="GGGAAACGCCTGGTATCTTT"
                     /Description="pBR322 origin, forward primer"
                     /X-Transferred_From="polyubi C promoter + mRFP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a href=""urn:local:.:br-etrdi3a"">polyubi C
                     promoter + mRFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a href=""urn:local:.:5j-etqqqce"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a href=""urn:local:.:ni-etrlssm"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a href=""urn:local:.:br-etrdi3a"">polyubi C
                     promoter + mRFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /label="pBR322ori-F"
     protein_bind    737..753
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="88.24%"
                     /Primary_Match="88.24%; Adjusted-Interval-0:0/0; lac
                     operator (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="88.24%; Adjusted-Interval-0:0/0; lac
                     operator (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="lac operator"
     primer_bind     741..764
                     /%GC="45.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /created_by="primer3"
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-R
                     (-46) (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13-R (-46) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /label="M13-R (-46)"
     primer_R        742..764
                     /created_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pLVX-LCK mNeongreen-IRES-puro"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:2b-hiwzq7q"">pLVX-LCK
                     mNeongreen-IRES-puro</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:x-hix6p2i"">Lifeact-mTFP1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:1l-hiwyywl"">pLVX-H2B
                     Electra2-IRES-puro</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:i-hix6kk2"">pLVX-LifeAct
                     mTFP1-IRES-puro</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lifeact-mTFP1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     R (<a href=""urn:local:.:j-hj6j0nq"">Lifeact-mTFP1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:3a-hhr5yby"">Lifeact-mVenus</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:1a-frb6qgg"">Tol2 Lox
                     H2BCerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:4i-fbvraag"">pGEM-5Zf(+)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:1n-hj6jtu7"">Lifeact-mhYFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:c-frx48cj"">Tol2
                     Transposase</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:g-g61d1og"">LCK_mScarlet</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:c-frb5cf6"">Piggybac Lox
                     H2B Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:28-hj6k5n3"">Lifeact-LSSmOrange</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     R (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /label="M13/pUC R"
     primer_bind     742..764
                     /Tm=58
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Description="In lacZ gene"
                     /X-Transferred_From="polyubi C promoter + mRFP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:br-etrdi3a"">polyubi C promoter +
                     mRFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a href=""urn:local:.:8o-etrc9l3"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a href=""urn:local:.:5j-etqqqce"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a href=""urn:local:.:ni-etrlssm"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     Reverse (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:br-etrdi3a"">polyubi C promoter +
                     mRFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a href=""urn:local:.:8o-etrc9l3"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     Reverse (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /label="M13/pUC Reverse"
     primer_R        761..777
                     /created_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<ahref=""urn:local:.:h-f47bj6f"">PhiC 31 Minos ITR MCS
                     Copy</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /label="M13R"
     misc_feature_   complement(761..777)
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_reverse (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_reverse (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /label="m13_reverse"
     primer_bind     761..777
                     /Tm=49
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Description="In lacZ gene. Also called M13-rev"
                     /X-Transferred_From="polyubi C promoter + mRFP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a href=""urn:local:.:br-etrdi3a"">polyubi C
                     promoter + mRFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Reverse (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Reverse (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Reverse (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Reverse (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Reverse (<a href=""urn:local:.:5j-etqqqce"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Reverse (<a href=""urn:local:.:ni-etrlssm"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a href=""urn:local:.:br-etrdi3a"">polyubi C
                     promoter + mRFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="M13 Reverse"
     primer_bind     761..777
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /X-Transferred_From="polyubi C promoter + mRFP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (<a href=""urn:local:.:br-etrdi3a"">polyubi C
                     promoter+ mRFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 rev (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 rev (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 rev (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 rev (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 rev (<a href=""urn:local:.:5j-etqqqce"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 rev (<a href=""urn:local:.:ni-etrlssm"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 rev
                     (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (<a href=""urn:local:.:br-etrdi3a"">polyubi C
                     promoter+ mRFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR
                     MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (<a href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK
                     in pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 rev
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="M13 rev"
     primer_bind     761..777
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC-GW-Amp.dna - L.pictus Nanos 3' and
                     5' w/o ORF - RFP_mChilada_Nanos_NPRE in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<ahref=""urn:local:.:ca-euqjr84"">pUC-GW-Amp.dna -
                     L.pictus Nanos 3' and 5' w/o ORF - RFP_mChilada_Nanos_NPRE
                     in pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R(<a
                     href=""urn:local:.:1r-euq9nvm"">pUC-GW-Amp.dna</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R(<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R(<a href=""urn:local:.:bk-euqjhhl"">pUC-GW-Amp.dna -
                     L.pictus Nanos 3' and 5' w/o ORF</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /label="M13R"
     primer_bind     761..777
                     /%GC="47.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="49.1"
                     /created_by="primer3"
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-R
                     (-26) (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13-R (-26) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /label="M13-R (-26)"
     repeat_region   complement(872..1125)
                     /note="derived from Minos transposon"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:50-es1dlm7"">PhiC 31 Minos ITR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="derived from Minos transposon"
     repeat_region   872..1125
                     /note="derived from Minos transposon"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:50-es1dlm7"">PhiC 31 Minos ITR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="99.61%"
                     /X-Primary_Match="99.61%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_mNeonGreen-pHEY1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="derived from Minos transposon"
     misc_feature    complement(968..990)
                     /X-Transferred_From="MiMIC_featured"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC_31_Minos_ITR_MCS in pTwist Amp
                     High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="gRNA_Target"
     primer_bind     1126..1159
                     /Tm=61
                     /Sequence="GCTACCTTAATCTCAAGAAGAGCAAAACAAAAGC"
                     /Description="<html><body></body></html>"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a href=""urn:local:.:50-es1dlm7"">PhiC 31
                     Minos ITR MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a href=""urn:local:.:13-esbocyl"">LoxP/Cre
                     Minos ITR MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="MiMIC_5'_for"
     protein_bind    1174..1207
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /label="loxP"
     protein_bind    complement(1174..1207)
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GGATACTT). <i>lox2272</i> sites are compatible
                     with each other, but incompatible with <i>loxP</i> or
                     <i>loxN</i> sites (<a
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/9714735"">Lee
                     and Saito, 1988</a>)."
                     /modified_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LoxP/Cre Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lox2272
                     (<a href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="82.35%"
                     /Primary_Match="82.35%; Adjusted-Interval-0:0/0; lox2272
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0; loxP
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="lox2272"
     Fragment        1221..4695
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:k-j3rualb@3.0#0#E1A8BFCCDB66B10D2698CF06B7CE28
                     9A label: Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter"
                     /Cloning_Method="Gibson Cloning"
                     /Fragment_Type="Insert"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_CFP-pHEY1"
                     /Transferred_Similarity="99.40%"
                     /Primary_Match="99.40%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (<a
                     href=""urn:local:.:37-j3rukov"">Lv_polyubiquitin_H2B_CFP-p
                     HEY1</a>)"
                     /Alternative_Match="99.40%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="99.40%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /Alternative_Match="99.40%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (insert)"
     promoter        1239..4677
                     /created_by="elliotjackson"
                     /X-Transferred_From="L.variegatus_polyubiquitinC in
                     pTwistAmp High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:br-etrdi3a"">polyubi C promoter +
                     mRFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:5w-eylipru"">H2B Cerulean plasmid
                     (backbone unknown) - L.variegatus_polyubiquitinC in
                     pTwistAmp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:84-ervi19a"">Genomic segment with
                     L.variegatus polyubiquitinC extraction. SEQUENCE TO
                     SYNTHESIZE</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RFP_mChilada_LCK in pCS2_8 -
                     L.variegatus_polyubiquitinC in pTwist Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:3n-ev0i2rr"">RFP_mChilada_LCK in
                     pCS2_8- L.variegatus_polyubiquitinC in pTwist Amp High
                     Copy</a>)"
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:h-j3ru7x7@2.0#0:[6,3445)#E1A8BFCCDB66B10D2698C
                     F06B7CE289A label: Lv_polyubiquitin_H2B_CFP: 7->3445"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:k-j3rualb"">Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter - PCR Product)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter - Digestion)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter - PCR Product)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter - Digestion)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:37-j3rukov"">Lv_polyubiquitin_H2B_CFP-p
                     HEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (pHEY1-H2B-mNeonGreen (digested by AsiSI, HindIII) -
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII)  - Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter)"
                     /label="L.variegatus polyubiquitin C promoter"
     primer_bind     1239..1263
                     /Sequence="ACTAGTCCTCACAACATTTCTTCCC"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Extension="GCCCGTCGACGCAATTGA"
                     /Tm_(binding_region)="60.5"
                     /Tm_(with_extension)="68.6"
                     /%GC_(binding_region)="44.0"
                     /%GC_(with_extension)="51.2"
                     /Hairpin_Tm_(with_extension)="36.1"
                     /Self_Dimer_Tm_(with_extension)="27.9"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - Digestion)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (1 F) - pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII) "
     primer_bind     1239..1263
                     /Sequence="ACTAGTCCTCACAACATTTCTTCCC"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Tm_(binding_region)="60.5"
                     /Tm_(with_extension)="68.6"
                     /%GC_(binding_region)="44.0"
                     /%GC_(with_extension)="51.2"
                     /Hairpin_Tm_(with_extension)="36.1"
                     /Self_Dimer_Tm_(with_extension)="27.9"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /Extension="GCCCGTCGACGCAATTGA"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (1 F) - pHEY1-H2B-mNeonGreen
                     (digested by AsiSI, HindIII)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5k-j47j94k"">Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (1 F) -
                     pHEY1-H2B-mNeonGreen (digested by AsiSI, HindIII)</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - Digestion)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (<a
                     href=""urn:local:.:37-j3rukov"">Lv_polyubiquitin_H2B_CFP-p
                     HEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (1 F) - pHEY1-H2B-mNeonGreen
                     (digested by AsiSI, HindIII)"
     primer_bind     complement(4658..4677)
                     /Sequence="GCGATCGCACTGACCTGTAG"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Tm_(binding_region)="60.9"
                     /Tm_(with_extension)="66.9"
                     /%GC_(binding_region)="60.0"
                     /%GC_(with_extension)="50.0"
                     /Hairpin_Tm_(with_extension)="49.4"
                     /Self_Dimer_Tm_(with_extension)="28.7"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /Extension="AATTCGAATCGATGCGAT"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (3439 R) - pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5m-j47j94k"">Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (3439 R) -
                     pHEY1-H2B-mCerulean (digested by AsiSI, HindIII)</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (3439 R) - pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII)"
     primer_bind     complement(4658..4677)
                     /Sequence="GCGATCGCACTGACCTGTAG"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Extension="AATTCGAATCGATGCGAT"
                     /Tm_(binding_region)="60.9"
                     /Tm_(with_extension)="66.9"
                     /%GC_(binding_region)="60.0"
                     /%GC_(with_extension)="50.0"
                     /Hairpin_Tm_(with_extension)="49.4"
                     /Self_Dimer_Tm_(with_extension)="28.7"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - Digestion)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (3439 R) - pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII) "
     primer_bind     complement(4658..4677)
                     /Sequence="GCGATCGCACTGACCTGTAG"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Tm_(binding_region)="60.9"
                     /Tm_(with_extension)="70.6"
                     /%GC_(binding_region)="60.0"
                     /%GC_(with_extension)="60.5"
                     /Hairpin_Tm_(with_extension)="48.8"
                     /Self_Dimer_Tm_(with_extension)="24.8"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /Extension="CTGGCTCTGGCATGCGAT"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (3439 R) - pHEY1-H2B-mNeonGreen
                     (digested by AsiSI, HindIII)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - Digestion)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (<a
                     href=""urn:local:.:37-j3rukov"">Lv_polyubiquitin_H2B_CFP-p
                     HEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5i-j47j94k"">Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (3439 R) -
                     pHEY1-H2B-mNeonGreen (digested by AsiSI, HindIII)</a>"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (3439 R) - pHEY1-H2B-mNeonGreen
                     (digested by AsiSI, HindIII)"
     CDS             4682..4999
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="GLRX"
     primer_bind     4682..4704
                     /Sequence="ATGGCTCAAGAGTTTGTGAACTG"
                     /created_by="primer3"
                     /Product_Size=1137
                     /Extension="AAAGGCCGGCCAGCG"
                     /Tm_(binding_region)="59.4"
                     /Tm_(with_extension)="69.9"
                     /%GC_(binding_region)="43.5"
                     /%GC_(with_extension)="55.3"
                     /Hairpin_Tm_(with_extension)="44.5"
                     /Self_Dimer_Tm_(with_extension)="41.4"
                     /Pair_Dimer_Tm_(binding_region)="1.5"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1 F) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1 F) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1 F) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (<a
                     href=""urn:local:.:cf-je2uv8b"">cyto Grx1-roGFP2</a>)"
                     /label="cyto Grx1-roGFP2 (1 F) - H2B Cerulean in pCS2+8
                     (digested by AsiSI, XbaI) Fragment 1"
     misc_feature    4682..5818
                     /Genetic_code="Standard"
                     /Reading_frame=1
                     /created_by="ORF Finder"
                     /modified_by="lesst"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:cf-je2uv8b"">cyto Grx1-roGFP2</a>"
                     /label="cyto Grx1-roGFP2"
     source          <4682..>5818
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; source
                     synthetic DNA construct (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     source synthetic DNA construct (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     source synthetic DNA construct (<a
                     href=""urn:local:.:cf-je2uv8b"">cyto Grx1-roGFP2</a>)"
     misc_feature    <4682..>5818
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:c0-je2us6k@1.0#0:[4097,5234)#A76A8D47849FB961F
                     470AA5F1C8DAF49 label: cyto-Grx1-roGFP2-pCS2+8  :
                     4098->5234"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto Grx1-roGFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (concatenated sequence 2) (<a
                     href=""urn:local:.:cf-je2uv8b"">cyto Grx1-roGFP2</a>)"
                     /Alternative_Match="99.23%; Adjusted-Interval-0:-15/19;
                     cyto Grx1-roGFP2 (concatenated sequence 2)
                     (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="99.23%; Adjusted-Interval-0:-15/19;
                     cyto Grx1-roGFP2 (concatenated sequence 2) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /label="cyto Grx1-roGFP2 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     5099..5116
                     /Sequence="ATGGTGAGCAAGGGCGAG"
                     /created_by="primer3"
                     /Product_Size=1314
                     /Extension="AAAGGCCGGCCAGCG"
                     /Tm_(binding_region)="59.7"
                     /Tm_(with_extension)="78.8"
                     /%GC_(binding_region)="61.1"
                     /%GC_(with_extension)="66.7"
                     /Hairpin_Tm_(with_extension)="58.1"
                     /Self_Dimer_Tm_(with_extension)="45.1"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="88.89%"
                     /Primary_Match="88.89%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1 F) - pCS2+8 (digested by AsiSI, XbaI) (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="88.89%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1 F) - pCS2+8 (digested by AsiSI, XbaI) (<a
                     href=""urn:local:.:co-je2uw6b"">cyto roGFP2-Orp1</a>)"
                     /Alternative_Match="88.89%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1 F) - pCS2+8 (digested by AsiSI, XbaI)
                     (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="cyto roGFP2-Orp1 (1 F) - pCS2+8 (digested by
                     AsiSI, XbaI)"
     CDS             5102..5818
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
                     /label="EGFP"
     primer_bind     complement(5799..5818)
                     /Sequence="TTACTTGTACAGCTCGTCCA"
                     /created_by="primer3"
                     /Product_Size=1137
                     /Extension="CGACTCACTATAGTTCTAG"
                     /Tm_(binding_region)="56.2"
                     /Tm_(with_extension)="62.5"
                     /%GC_(binding_region)="45.0"
                     /%GC_(with_extension)="43.6"
                     /Hairpin_Tm_(with_extension)="38.3"
                     /Self_Dimer_Tm_(with_extension)="9.2"
                     /Pair_Dimer_Tm_(binding_region)="1.5"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (<a
                     href=""urn:local:.:cf-je2uv8b"">cyto Grx1-roGFP2</a>)"
                     /label="cyto Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8
                     (digested by AsiSI, XbaI) Fragment 1"
     ligation        5819..5837
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Ligation
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /label="Ligation"
     primer_bind     complement(5823..5842)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="4.4"
                     /Tm="50.3"
                     /created_by="primer3"
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Mismatch_Positions="==================GG"
                     /Mismatches=2
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /label="T7 promoter"
     promoter        complement(5825..5842)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.7"
                     /fragment="True"
                     /other="promoter"
                     /label="T7 promoter (fragment)"
     primer_bind     complement(5825..5841)
                     /%GC="35.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="44.2"
                     /created_by="primer3"
                     /Sequence="ATTAACCCTCACTAAAG"
                     /Mismatch_Positions="=T==ACC=======A=="
                     /Mismatches=5
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /label="T3 promoter (17bp)"
     polyA_signal    complement(5847..5981)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /label="SV40 poly(A) signal"
     polyA_signal    5847..5981
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="SV40 poly(A) signal"
     polyA_signal    5847..5981
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="RFP_mChilada_LCK in pCS2_8"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:3n-ev0i2rr"">mChilada_LCK Lv
                     polyubiquitinC</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:5w-eylipru"">H2B Cerulean plasmid-
                     Lv_polyubiquitinC</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:4z-eylieh4"">H2B Cerulean plasmid
                     (backbone unknown) - pabp in pUC-GW-Amp</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5871..5890)
                     /Tm=54
                     /Sequence="GTGGTTTGTCCAAACTCATC"
                     /Description="SV40 polyA terminator, reverse primer"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; EBV-rev
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EBV-rev (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="EBV-rev"
     primer_bind     5925..5944
                     /Tm=51
                     /Sequence="GAAATTTGTGATGCTATTGC"
                     /Description="SV40 polyA, reverse primer"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40pA-R
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40pA-R (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="SV40pA-R"
     protein_bind    6005..6038
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /label="lox2272"
     protein_bind    complement(6005..6038)
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GGATACTT). <i>lox2272</i> sites are compatible
                     with each other, but incompatible with <i>loxP</i> or
                     <i>loxN</i> sites (<a
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/9714735"">Lee
                     and Saito, 1988</a>)."
                     /modified_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LoxP/Cre Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lox2272
                     (<a href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="82.35%"
                     /Primary_Match="82.35%; Adjusted-Interval-0:0/0; lox2272
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0; loxP
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="lox2272"
     primer_bind     complement(6039..6072)
                     /Tm=59
                     /Sequence="CTTAAAACAATAATTTAATTAATTTCCCCCGCGG"
                     /Description="<html><body></body></html>"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a href=""urn:local:.:50-es1dlm7"">PhiC 31
                     Minos ITR MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a href=""urn:local:.:13-esbocyl"">LoxP/Cre
                     Minos ITR MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="MiMIC_3'_rev"
     repeat_region   complement(6090..6342)
                     /note="derived from Minos transposon"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:50-es1dlm7"">PhiC 31 Minos ITR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="derived from Minos transposon"
     repeat_region   6090..6342
                     /note="derived from Minos transposon"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:50-es1dlm7"">PhiC 31 Minos ITR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="99.61%"
                     /X-Primary_Match="99.61%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lv_polyubiquitin_H2B_mNeonGreen-pHEY1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="derived from Minos transposon"
     misc_feature    6225..6247
                     /X-Transferred_From="MiMIC_featured"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC_31_Minos_ITR_MCS in pTwist Amp
                     High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="gRNA_Target"
     CDS             6433..6476
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="25.3"
                     /fragment="True"
                     /other="CDS"
                     /label="lacZα (fragment)"
     primer_bind     complement(6433..6450)
                     /Tm=56
                     /Sequence="TGTAAAACGACGGCCAGT"
                     /Description="In lacZ gene. Also called M13-F20 or M13
                     (-21) Forward"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Forward (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Forward (<a href=""urn:local:.:5j-etqqqce"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Forward (<a href=""urn:local:.:ni-etrlssm"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /label="M13 Forward"
     primer_bind     complement(6433..6449)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR
                     MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:5j-etqqqce"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:ni-etrlssm"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 fwd
                     (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR
                     MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 fwd
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="M13 fwd"
     primer_R        complement(6434..6449)
                     /created_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13F
                     (<ahref=""urn:local:.:h-f47bj6f"">PhiC 31 Minos ITR MCS
                     Copy</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13F (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="M13F"
     misc_feature_   complement(6434..6449)
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_forward (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="m13_forward"
     primer_bind     complement(6442..6464)
                     /Tm=61
                     /Sequence="CCCAGTCACGACGTTGTAAAACG"
                     /Description="In lacZ gene"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a href=""urn:local:.:8o-etrc9l3"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a href=""urn:local:.:5j-etqqqce"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a href=""urn:local:.:ni-etrlssm"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     Forward (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a href=""urn:local:.:8o-etrc9l3"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     Forward (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="M13/pUC Forward"
     primer_bind     complement(6453..6476)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC-GW-Amp.dna"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13F(-47) (<a
                     href=""urn:local:.:1r-euq9nvm"">pUC-GW-Amp.dna</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13F(-47) (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13F(-47) (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="M13F(-47)"
     promoter        6563..6667
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     misc_feature_   complement(6563..6667)
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="H2B_GFP_mAbbsynth in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:58-epr10bc"">H2B_GFP_mAbbsynth in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:5o-epr10cb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:h-hh7co6e"">Lifeact-mCherry in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a href=""urn:local:.:1j-he1rucf"">Minos
                     Lox Lvpolyubi LCK mNeongreen</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:g-hgwqs99"">pCS2FA-transposase</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a href=""urn:local:.:n-eurzkb4"">Pictus
                     Nanos 5' and 3' UTR MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="AmpR_promoter"
     primer_bind     6624..6640
                     /%GC="35.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="44.2"
                     /created_by="primer3"
                     /Sequence="ATTAACCCTCACTAAAG"
                     /Mismatch_Positions="=T=======C=CT==A="
                     /Mismatches=5
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /label="T3 promoter (17bp)"
     misc_feature    6668..7528
                     /note="/application_notes=Allows E. coli to be resistant
                     to ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=39ee7c4ce93d4075b0df0df5df6b9861"
                     /X-Transferred_From="Minos Lox H2B Cerulean Lv
                     polyubiquitinC"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a href=""urn:local:.:t-fgi9koc"">Minos Lox
                     H2B Cerulean Lv polyubiquitinC</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:g-gkc2nm4"">hCas9-T2A-H2B
                     mCitrine</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:o-hfwuxxr"">EMTB-mCherry</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a href=""urn:local:.:c-fkl7ij5"">Piggybac
                     Transposase</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a
                     href=""urn:local:.:h-h1rgjqn"">pSFFV_mNG2(11)1-10
                     (reversed)</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a href=""urn:local:.:c-frx48cj"">Tol2
                     Transposase</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a
                     href=""urn:local:.:i-he1qclb"">memb_CER_JPC_05
                     (reversed)</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a href=""urn:local:.:1b-fqqgbta"">HyBPase
                     tranposase</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a href=""urn:local:.:1j-he1rucf"">Minos Lox
                     Lvpolyubi LCK mNeongreen</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a href=""urn:local:.:t-fgi9koc"">Minos Lox
                     H2B Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:1n-hj6jtu7"">Lifeact-mhYFP</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:28-hj6k5n3"">Lifeact-LSSmOrange</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:1g-hhr5igo"">Lifeact-mTFP1</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:h-hh7co6e"">Lifeact-mCherry in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:g-gkc2nm4"">hCas9-T2A-H2B
                     mCitrine</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:2q-hj6khyv"">H2B-Electra2</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:14-hj6jb5q"">Lifeact-mRuby</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="AmpiciIIin"
     CDS             6668..7528
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
     misc_feature_   complement(6668..7528)
                     /X-Transferred_From="H2B_GFP_mAbbsynth in pCS2_8"
                     /X-Transferred_Similarity="99.88%"
                     /X-Primary_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:58-epr10bc"">H2B_GFP_mAbbsynth in
                     pCS2_8</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a
                     href=""urn:local:.:5o-epr10cb"">YFP_mAbbsynth_LCKin
                     pCS2_8</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK
                     in pCS2_8</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a href=""urn:local:.:n-eurzkb4"">Pictus Nanos 5'
                     and 3' UTR MCS</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a href=""urn:local:.:o-hfwuxxr"">EMTB-mCherry</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ampR
                     (<ahref=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCK
                     mCherry</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:58-epr10bc"">H2B_GFP_mAbbsynth in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:5o-epr10cb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:h-hh7co6e"">Lifeact-mCherry in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a
                     href=""urn:local:.:g-hgwqs99"">pCS2FA-transposase</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:n-eurzkb4"">Pictus Nanos 5' and
                     3'UTR MCS</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:o-hfwuxxr"">EMTB-mCherry</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ampR (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /label="ampR"
     primer_bind     complement(6886..6905)
                     /Tm=58
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /Description="Ampicillin resistance gene, reverse primer"
                     /X-Transferred_From="polyubi C promoter + mRFP"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Amp-R
                     (<a href=""urn:local:.:br-etrdi3a"">polyubi C promoter +
                     mRFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCKin
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a href=""urn:local:.:5j-etqqqce"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a href=""urn:local:.:ni-etrlssm"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Amp-R
                     (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a href=""urn:local:.:br-etrdi3a"">polyubi C
                     promoter + mRFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCKin
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE
                     inpCS2_8</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Amp-R
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /label="Amp-R"
     attL1           7553..7649
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL1
                     (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:2e-ggvr0c6"">Minos_lox_LvEndo16_LCKmChe
                     rry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a href=""urn:local:.:t-fgi9koc"">Minos Lox H2B
                     Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
     attL1           7553..7649
     attP1           7554..7753
                     /X-Transferred_From="Minos Lox H2B Cerulean Lv
                     polyubiquitinC"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP1
                     (<a href=""urn:local:.:t-fgi9koc"">Minos Lox H2B Cerulean
                     Lv polyubiquitinC</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP1
                     (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a href=""urn:local:.:1j-he1rucf"">Minos Lox
                     Lvpolyubi LCK mNeongreen</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a href=""urn:local:.:t-fgi9koc"">Minos Lox H2B
                     Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
     attP1           7554..7753
     attB1           7628..7652
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:1/-3;
                     attB1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:1/-3;
                     attB1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:1/-3;
                     attB1 (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
     attB1           7628..7652
     attR1           7628..7753
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1
                     (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a href=""urn:local:.:t-fgi9koc"">Minos Lox H2B
                     Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
     attR1           7628..7753
     protein_bind    7628..7652
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /label="attB1"
     misc_feature_   complement(7628..7652)
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_Lox_ITR_MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /label="attB1"
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttccagc
      661 gacatatgcc atacgccgaa ctgaactcca cctaatgtct tggagattgt cggatagcgc
      721 ggaataggat tgtgccctct gagcggataa caatttcaca caggaaacag ctatgaccat
      781 gacacatgca tccaccatcg cagacttatc atcactgagc ctccacctag cctcattctc
      841 tatgctagct taagatattt ttcgttttct acgagcccca accactatta attcgaacag
      901 catgtttttt ttgcagtgcg caatgtttaa cacactatat tatcaatact actaaagata
      961 acacatacca atgcatttcg tctcaaagag aattttattc tcttcacgac gaaaaaaaaa
     1021 gttttgctct atttccaaca acaacaaaaa tatgagtaat ttattcaaac ggtttgctta
     1081 agagataaga aaaaagtgac cactattaat tcgaacgcgg cgtaagctac cttaatctca
     1141 agaagagcaa aacaaaagca actaatgtaa cggataactt cgtatagcat acattatacg
     1201 aagttataac catatgtgac gtctacggat ccgcatgcac tagtcctcac aacatttctt
     1261 cccttttttg cgggagttgg ggcaaataat cctgacaatg tccaatgatt attttaaaaa
     1321 gtgctatgta caatcgcttt gtatttgtat aattgttata aaaaatgcat tgtatgtatt
     1381 tatatttaca tacataatta ttctacaaag tgtattgtac aatcacttta tatcaacttt
     1441 agatgtatcg tgttattagt gtcaaatctg actgcgtgca tactgattcg atgccaaaag
     1501 tggattaatg cctccttgtg ccccgcgtgt ctcttggcaa ctagagcatt attgtagaat
     1561 attggggcac ctaaaataca ctgacaagca atcatccaaa aatatctaac aaaatactaa
     1621 acaataacag gacggtcatt tttctgatat ctaaaacaag tttttgttga agggacattg
     1681 tgcaagactt tcaaaaactt attaagaatg ttgaaccgtt tacttttttt attttaacac
     1741 gtgatactat agtatacata acgattgggc gaaaaaatac cgtgacagtc aatcaaacaa
     1801 tctaaattcc cactttgcag aatcactttt gtgattccct tgtatcttca acaagatata
     1861 gatagatgga tagcatggaa gtttttgttt tttacttaga aatggtaaaa cgatgttttt
     1921 tccccagggt cttttccccc tatgtaaatt atgtttcatt ctttcaataa aaaaaaacac
     1981 aaactgaaaa aaaacttata agcaaaaaaa aaggcgtaaa tattttttta tgaactctgt
     2041 taaacggaaa caacaacaac acagtcgcca atatatacct cccgcgacca gtgatcattc
     2101 cagaaaatgt tttgggccga aattatattg cataattccg aaattgcgag tcatcatcgt
     2161 ctgcaatatc tggcgcgggt taatatgacg tcatgtgcca tagatacaca atctatcttt
     2221 attacccaat agaaaaggat gaaatagtcc atgtgacaca gactgaccaa tcgcaagtca
     2281 acacaaagca ccatataaaa cacacgcaag cgtcgcagtg tcaataaatt gcatgagact
     2341 tccgcagagg ttgcactcga gaagaactcg tccatttttt tccagaaaaa ctctaatttc
     2401 aaagtaagta gacgaggttt tcacattatt ttaaggcagt tatttcaatg aagatgcagt
     2461 ttgtaaatgt ttgatttaaa aaaagcactt ttgaagaaga atgatgatga agaacataaa
     2521 ttgtcaacga ccacatcgtc gtcattattc gtgtgctttt tttttgttgt aatttatgtt
     2581 tggctttcca aatgaaggca atataggaac attaacagca tgaacaaagt ctcatattta
     2641 gtttgattga aagttcaaat tatagggcgt agaaatcatt gaaattgtag ggtggatacc
     2701 gtggattatt gcgtcgtgtg tgtgcatgat aagatcgtct gactcagacg tcgcgtcttt
     2761 tgttgtgtgg tgtgtgtggt atggatgatg atcaattgtg atgtttggtg gggattttca
     2821 tgaacgctga attaaaagac aatgagtgga aatggccatg ccaatttctg gtccatggat
     2881 tgtgtgaatg caatgacaat ttcattcaat ttttgtctgg gctgccatga tgaacatgat
     2941 gaagtctgaa ctgaagtgtt gtgattgaaa tgacaaagaa tttttgtcat gagaaagaat
     3001 gaggcatgta cggtacggta acttaaaacg ttgatagcta ggtgtagatt ttctagatac
     3061 gtttggcagt aggcctagat ctagaggcac ttggccaatc atgaatagtc tatgggagac
     3121 aatttcccat attgaagact tgctttgcaa aagaaacgac accaacaaag cgtaattttt
     3181 tccaccccaa attttttcgc gaaggtcaga acagaagccc atggcaaagg cctgttttgt
     3241 atgtgcatgg ccatggatga aaaaaatata aatacaaaag ttggtgaatt tttaaagatg
     3301 aaaaggggta atttttcatg aggtatctgc ttaggcccta tcacattttt atttgctgcc
     3361 aaggtaattc ttttgctgca tgtgtaaaaa aaggaaattg gtctcccaaa ctgggagtgg
     3421 agactgtctc tgaaattgga tacccaagtt ctttaatctt tacaaaattc tgatattgaa
     3481 aataatctct ctcatgggag atgttctccc atattggctg tgcaccagtt ggtcggtgtg
     3541 caaagtctag acaatacgta tccaggcact atcgcctatg aataatgcaa tagcagtgca
     3601 gttcacaacg gcctctctcc cgatcagcgc agatgttggg gaactaattt caacttttgt
     3661 cgtaatttat gtcaaataac tgacagtcgt aacttaagac cctaatcagc taatgttaat
     3721 ccttgacctg aattgcaggc atctgaaacg gctgtaatcc agcttcgatc ttaatgtcat
     3781 agttcaaatt ctcagtagat ttaactgagc gtggtgcgtg tttggccatt gccatagttt
     3841 ttatatggac tgaagtcaac gaccaaaaga tctgctcaag gcagcccaac tgcattagca
     3901 agattttggt tgctattcag tcaatatcag aactacggca atggacgagt gcggcgatgt
     3961 caacctacat gtaactgaga attcaaacta tgacattgag aacgaagcca aaatacagca
     4021 aatattgatg tggttgaaac ttgggtcaag gatcaaatgg acaatatttg gcaaaaaaga
     4081 taaattttga ggtgaaattg tttcctgaca ttgccatgtt ggttggtgtg aaactgcagc
     4141 gcaagacaat gaaggaagag actttataga gtaagctgcc ccgaatttga catgttttga
     4201 accatttttt gttcttaagt cttttcacat aatgaaaatt gcattaaaat gatcaatgtt
     4261 ggttgaatat atttagaatt gcaatgttga atttctccat tttgaatatt tcataaaaaa
     4321 tctgcactgt accaccatgt tttgctcccg ccacagattt cttaatttta gagcatttta
     4381 tggctacctc atttcttaaa tctgagcaaa gctaccgcat gttattgaaa tatggcacag
     4441 attcgcattt ttaaagggga ttttggtgtt caagtcgtga cttgcatgtg gtgggtgata
     4501 atgcctgtgt atgtcaatgc atgtggttcg tgttgtgtgg attgactgag ctgacttgtg
     4561 gaataagttt gccatgttga attccaggca tgctactctg aataaaggtc tttagaaaaa
     4621 cagattccaa taatgttttc atttttttgt caattttcta caggtcagtg cgatcgcatc
     4681 gatggctcaa gagtttgtga actgcaaaat ccagcctggg aaggtggttg tgttcatcaa
     4741 gcccacctgc ccgtactgca ggagggccca agagatcctc agtcaattgc ccatcaaaca
     4801 agggcttctg gaatttgtcg atatcacagc caccaaccac actaacgaga ttcaagatta
     4861 tttgcaacag ctcacgggag caagaacggt gcctcgagtc tttattggta aagattgtat
     4921 aggcggatgc agtgatctag tctctttgca acagagtggg gaactgctga cgcggctaaa
     4981 gcagattgga gctctgcaga ctagtggtgg ttcaggtggt ggtggttcag gtggtggtgg
     5041 ttcaggtgga ggaggatcag gaggaggagg atcaggagga ggaggatcag gaggagaatt
     5101 cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     5161 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     5221 caagctgacc ctgaagttca tctccaccac cggcaagctg cccgtgccct ggcccaccct
     5281 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     5341 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     5401 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     5461 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     5521 gctggagtac aactacaact gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     5581 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     5641 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     5701 cctgagcacc tgctccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     5761 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaact
     5821 agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg
     5881 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     5941 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggccgcctt cacgcgtggt
     6001 taccataact tcgtatagga tactttatac gaagttatcc gcgggggaaa ttaattaaat
     6061 tattgtttta agtatgatag taaatcacat tacgccgcgt tcgaattaat agtggtcact
     6121 tttttcttat ctcttaagca aaccgtttga ataaattact catatttttg ttgttgttgg
     6181 aaatagagca aaactttttt tttcgtcgtg aagagaataa aattctcttt gagacgaaat
     6241 gcattggtat gtgttatctt tagtagtatt gataatatag tgtgttaaac attgcgcact
     6301 gcaaaaaaac atgctgttcg aattaatagt ggttggggct cgtagaagct gctaacgtgc
     6361 ttggcataat ttatagaagc aggtcaggcg gaatggcact tccttattcc agatgcgtgc
     6421 ggattatgca tgactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgcttg
     6481 tggtggatca cagttattgg agacttcctt ggccgtacga tgggttttgt ttcaggtatc
     6541 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6601 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6661 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6721 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6781 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6841 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6901 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6961 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7021 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7081 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7141 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7201 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7261 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7321 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7381 tggctctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7441 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7501 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7561 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc ttgaattgcc
     7621 attatcaaca agtttgtaca aaaaagcagg ctccgaattg gtatcacgag gacgatttga
     7681 agccccctac ccatttacgc tgacttgacg ggacggcggc tttgttgaat agagcgtcag
     7741 accccgtaga aaagatcaaa ggatcttc
//
