LOCUS       LvPolyubiquitinC-roGFP2-Orp1-lox-minos        7945 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  1-orp_01.
ACCESSION   1-orp_01
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     misc_feature_   complement(1..589)
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="H2B_GFP_mAbbsynth in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:58-epr10bc"">H2B_GFP_mAbbsynth in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:5o-epr10cb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a href=""urn:local:.:w-g2s1x8b"">mGreen
                     Lantern</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:h-hh7co6e"">Lifeact-mCherry in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a href=""urn:local:.:1j-he1rucf"">Minos
                     Lox Lvpolyubi LCK mNeongreen</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:n-eurzkb4"">PictusNanos 5' and 3' UTR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:o-hfwuxxr"">EMTB-mCherry</a>)"
                     /Alternative_Match="99.83%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:g-hgwqs99"">pCS2FA-transposase</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     colE1_high_copy (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="colE1_high_copy"
     primer_bind     490..509
                     /Tm=56
                     /Sequence="GGGAAACGCCTGGTATCTTT"
                     /Description="pBR322 origin, forward primer"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="pBR322ori-F"
     protein_bind    737..753
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /X-Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /X-Transferred_Similarity="88.24%"
                     /X-Primary_Match="88.24%; Adjusted-Interval-0:0/0; lac
                     operator (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /X-Alternative_Match="88.24%; Adjusted-Interval-0:0/0; lac
                     operator (cyto-roGFP2-Orp1-pCS2+8)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lac
                     operator (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="88.24%; Adjusted-Interval-0:0/0; lac
                     operator (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="88.24%; Adjusted-Interval-0:0/0; lac
                     operator (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="lac operator"
     primer_bind     741..764
                     /%GC="45.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /created_by="primer3"
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-R
                     (-46) (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13-R (-46) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13-R (-46) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13-R (-46)"
     primer_bind     742..764
                     /Tm=58
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Description="In lacZ gene"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     Reverse (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13/pUC Reverse"
     primer_R        742..764
                     /created_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pLVX-LCK mNeongreen-IRES-puro"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:2b-hiwzq7q"">pLVX-LCK
                     mNeongreen-IRES-puro</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:x-hix6p2i"">Lifeact-mTFP1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:1l-hiwyywl"">pLVX-H2B
                     Electra2-IRES-puro</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:i-hix6kk2"">pLVX-LifeAct
                     mTFP1-IRES-puro</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lifeact-mTFP1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     R (<a href=""urn:local:.:j-hj6j0nq"">Lifeact-mTFP1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:3a-hhr5yby"">Lifeact-mVenus</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:1a-frb6qgg"">Tol2 Lox
                     H2BCerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:4i-fbvraag"">pGEM-5Zf(+)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:1n-hj6jtu7"">Lifeact-mhYFP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:c-frx48cj"">Tol2
                     Transposase</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:g-g61d1og"">LCK_mScarlet</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a href=""urn:local:.:c-frb5cf6"">Piggybac Lox
                     H2B Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:28-hj6k5n3"">Lifeact-LSSmOrange</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC R (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     R (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13/pUC R"
     primer_bind     761..777
                     /Tm=49
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Description="In lacZ gene. Also called M13-rev"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Reverse (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13 Reverse"
     primer_bind     761..777
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 rev
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     rev (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13 rev"
     primer_bind     761..777
                     /%GC="47.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="49.1"
                     /created_by="primer3"
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13-R
                     (-26) (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13-R (-26) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13-R (-26) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13-R (-26)"
     primer_bind     761..777
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC-GW-Amp.dna - L.pictus Nanos 3' and
                     5' w/o ORF - RFP_mChilada_Nanos_NPRE in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<ahref=""urn:local:.:ca-euqjr84"">pUC-GW-Amp.dna -
                     L.pictus Nanos 3' and 5' w/o ORF - RFP_mChilada_Nanos_NPRE
                     in pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R(<a
                     href=""urn:local:.:1r-euq9nvm"">pUC-GW-Amp.dna</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R(<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R(<a href=""urn:local:.:bk-euqjhhl"">pUC-GW-Amp.dna -
                     L.pictus Nanos 3' and 5' w/o ORF</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13R"
     primer_R        761..777
                     /created_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<ahref=""urn:local:.:h-f47bj6f"">PhiC 31 Minos ITR MCS
                     Copy</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13R"
     misc_feature_   complement(761..777)
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_reverse (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_reverse (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_reverse (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="m13_reverse"
     repeat_region   complement(872..1125)
                     /note="derived from Minos transposon"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:50-es1dlm7"">PhiC 31 Minos ITR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="derived from Minos transposon"
     repeat_region   872..1125
                     /note="derived from Minos transposon"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:50-es1dlm7"">PhiC 31 Minos ITR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_mNeonGreen-pHEY1
                     "
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="derived from Minos transposon"
     misc_feature    complement(968..990)
                     /X-Transferred_From="MiMIC_featured"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC_31_Minos_ITR_MCS in pTwist Amp
                     High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="gRNA_Target"
     primer_bind     1126..1159
                     /Tm=61
                     /Sequence="GCTACCTTAATCTCAAGAAGAGCAAAACAAAAGC"
                     /Description="<html><body></body></html>"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a href=""urn:local:.:50-es1dlm7"">PhiC 31
                     Minos ITR MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a href=""urn:local:.:13-esbocyl"">LoxP/Cre
                     Minos ITR MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_5'_for (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="MiMIC_5'_for"
     protein_bind    1174..1207
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /label="loxP"
     protein_bind    complement(1174..1207)
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GGATACTT). <i>lox2272</i> sites are compatible
                     with each other, but incompatible with <i>loxP</i> or
                     <i>loxN</i> sites (<a
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/9714735"">Lee
                     and Saito, 1988</a>)."
                     /modified_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LoxP/Cre Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lox2272
                     (<a href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="82.35%"
                     /X-Primary_Match="82.35%; Adjusted-Interval-0:0/0; lox2272
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="82.35%; Adjusted-Interval-0:0/0;
                     loxP (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lox2272
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="94.12%; Adjusted-Interval-0:0/0;
                     lox2272 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0;
                     lox2272 (<a href=""urn:local:.:de-je2v13m"">1-orp_01</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0;
                     lox2272 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0; loxP
                     (<a href=""urn:local:.:de-je2v13m"">1-orp_01</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0; loxP
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="lox2272"
     Fragment        1221..4695
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:k-j3rualb@3.0#0#E1A8BFCCDB66B10D2698CF06B7CE28
                     9A label: Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter"
                     /Cloning_Method="Gibson Cloning"
                     /Fragment_Type="Insert"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_CFP-pHEY1"
                     /X-Transferred_Similarity="99.40%"
                     /X-Primary_Match="99.40%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (<a
                     href=""urn:local:.:37-j3rukov"">Lv_polyubiquitin_H2B_CFP-p
                     HEY1</a>)"
                     /X-Alternative_Match="99.40%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="99.40%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /X-Alternative_Match="99.40%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="99.77%"
                     /Primary_Match="99.77%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (insert) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (insert)"
     promoter        1239..4677
                     /created_by="elliotjackson"
                     /X-Transferred_From="L.variegatus_polyubiquitinC in
                     pTwistAmp High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:br-etrdi3a"">polyubi C promoter +
                     mRFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:5w-eylipru"">H2B Cerulean plasmid
                     (backbone unknown) - L.variegatus_polyubiquitinC in
                     pTwistAmp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:84-ervi19a"">Genomic segment with
                     L.variegatus polyubiquitinC extraction. SEQUENCE TO
                     SYNTHESIZE</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RFP_mChilada_LCK in pCS2_8 -
                     L.variegatus_polyubiquitinC in pTwist Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:3n-ev0i2rr"">RFP_mChilada_LCK in
                     pCS2_8- L.variegatus_polyubiquitinC in pTwist Amp High
                     Copy</a>)"
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:h-j3ru7x7@2.0#0:[6,3445)#E1A8BFCCDB66B10D2698C
                     F06B7CE289A label: Lv_polyubiquitin_H2B_CFP: 7->3445"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:k-j3rualb"">Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter - PCR Product)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter - Digestion)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter - PCR Product)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter - Digestion)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:37-j3rukov"">Lv_polyubiquitin_H2B_CFP-p
                     HEY1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter
                     (pHEY1-H2B-mNeonGreen (digested by AsiSI, HindIII) -
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII)  - Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="L.variegatus polyubiquitin C promoter"
     primer_bind     1239..1263
                     /Sequence="ACTAGTCCTCACAACATTTCTTCCC"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Extension="GCCCGTCGACGCAATTGA"
                     /Tm_(binding_region)="60.5"
                     /Tm_(with_extension)="68.6"
                     /%GC_(binding_region)="44.0"
                     /%GC_(with_extension)="51.2"
                     /Hairpin_Tm_(with_extension)="36.1"
                     /Self_Dimer_Tm_(with_extension)="27.9"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter - PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - Digestion)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (1 F) - pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII) "
     primer_bind     1239..1263
                     /Sequence="ACTAGTCCTCACAACATTTCTTCCC"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Tm_(binding_region)="60.5"
                     /Tm_(with_extension)="68.6"
                     /%GC_(binding_region)="44.0"
                     /%GC_(with_extension)="51.2"
                     /Hairpin_Tm_(with_extension)="36.1"
                     /Self_Dimer_Tm_(with_extension)="27.9"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /Extension="GCCCGTCGACGCAATTGA"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (1 F) -
                     pHEY1-H2B-mNeonGreen (digested by AsiSI, HindIII)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5k-j47j94k"">Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (1 F) -
                     pHEY1-H2B-mNeonGreen (digested by AsiSI, HindIII)</a>"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - Digestion)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (<a
                     href=""urn:local:.:37-j3rukov"">Lv_polyubiquitin_H2B_CFP-p
                     HEY1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (1 F) - pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (1 F) - pHEY1-H2B-mNeonGreen
                     (digested by AsiSI, HindIII)"
     primer_bind     complement(4658..4677)
                     /Sequence="GCGATCGCACTGACCTGTAG"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Tm_(binding_region)="60.9"
                     /Tm_(with_extension)="66.9"
                     /%GC_(binding_region)="60.0"
                     /%GC_(with_extension)="50.0"
                     /Hairpin_Tm_(with_extension)="49.4"
                     /Self_Dimer_Tm_(with_extension)="28.7"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /Extension="AATTCGAATCGATGCGAT"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (3439 R) -
                     pHEY1-H2B-mCerulean (digested by AsiSI, HindIII)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5m-j47j94k"">Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (3439 R) -
                     pHEY1-H2B-mCerulean (digested by AsiSI, HindIII)</a>"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (3439 R) - pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII)"
     primer_bind     complement(4658..4677)
                     /Sequence="GCGATCGCACTGACCTGTAG"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Extension="AATTCGAATCGATGCGAT"
                     /Tm_(binding_region)="60.9"
                     /Tm_(with_extension)="66.9"
                     /%GC_(binding_region)="60.0"
                     /%GC_(with_extension)="50.0"
                     /Hairpin_Tm_(with_extension)="49.4"
                     /Self_Dimer_Tm_(with_extension)="28.7"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter - PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - Digestion)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (pHEY1-H2B-mCerulean (digested by AsiSI,
                     HindIII)  - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mCerulean (digested by
                     AsiSI, HindIII)  (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (3439 R) - pHEY1-H2B-mCerulean
                     (digested by AsiSI, HindIII) "
     primer_bind     complement(4658..4677)
                     /Sequence="GCGATCGCACTGACCTGTAG"
                     /created_by="primer3"
                     /Product_Size=3439
                     /Tm_(binding_region)="60.9"
                     /Tm_(with_extension)="70.6"
                     /%GC_(binding_region)="60.0"
                     /%GC_(with_extension)="60.5"
                     /Hairpin_Tm_(with_extension)="48.8"
                     /Self_Dimer_Tm_(with_extension)="24.8"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /Extension="CTGGCTCTGGCATGCGAT"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (3439 R) -
                     pHEY1-H2B-mNeonGreen (digested by AsiSI, HindIII)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - PCR Product)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter - Digestion)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (<a
                     href=""urn:local:.:37-j3rukov"">Lv_polyubiquitin_H2B_CFP-p
                     HEY1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (pHEY1-H2B-mNeonGreen (digested by AsiSI,
                     HindIII) - Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5i-j47j94k"">Lv_polyubiquitin_H2B_CFP -
                     L.variegatus polyubiquitin C promoter (3439 R) -
                     pHEY1-H2B-mNeonGreen (digested by AsiSI, HindIII)</a>"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Lv_polyubiquitin_H2B_CFP - L.variegatus polyubiquitin C
                     promoter (3439 R) - pHEY1-H2B-mNeonGreen (digested by
                     AsiSI, HindIII) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="Lv_polyubiquitin_H2B_CFP - L.variegatus
                     polyubiquitin C promoter (3439 R) - pHEY1-H2B-mNeonGreen
                     (digested by AsiSI, HindIII)"
     CDS             4682..5398
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
                     /label="EGFP"
     misc_feature    <4682..>5995
                     /Color="#00ffff"
                     /created_by="byl003"
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:c4-je2us6k@1.0#0:[1159,2473)#AB811B44316EBD460
                     54511295713F7EF label: cyto-roGFP2-Orp1-pCS2+8:
                     1160->2473"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto roGFP2-Orp1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ORP1-ROGFP2-SV40-POLYA (<a
                     href=""urn:local:.:co-je2uw6b"">cyto roGFP2-Orp1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:co-je2uw6b"">cyto roGFP2-Orp1</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="98.54%; Adjusted-Interval-0:-15/179;
                     ORP1-ROGFP2-SV40-POLYA (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="98.54%; Adjusted-Interval-0:-15/179;
                     ORP1-ROGFP2-SV40-POLYA (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="ORP1-ROGFP2-SV40-POLYA"
     source          <4682..>5995
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; source
                     synthetic DNA construct (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     source synthetic DNA construct (<a
                     href=""urn:local:.:co-je2uw6b"">cyto roGFP2-Orp1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     source synthetic DNA construct (cyto-roGFP2-Orp1-pCS2+8)"
     misc_feature    <4682..>5995
                     /Source_Sequence="referenceToDocument:
                     urn:local:.:c4-je2us6k@1.0#0:[1159,2473)#AB811B44316EBD460
                     54511295713F7EF label: cyto-roGFP2-Orp1-pCS2+8:
                     1160->2473"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto roGFP2-Orp1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (concatenated sequence 2) (<a
                     href=""urn:local:.:co-je2uw6b"">cyto roGFP2-Orp1</a>)"
                     /Alternative_Match="99.33%; Adjusted-Interval-0:-15/19;
                     cyto roGFP2-Orp1 (concatenated sequence 2) (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="99.33%; Adjusted-Interval-0:-15/19;
                     cyto roGFP2-Orp1 (concatenated sequence 2)
                     (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="cyto roGFP2-Orp1 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     4682..4699
                     /Sequence="ATGGTGAGCAAGGGCGAG"
                     /created_by="primer3"
                     /Product_Size=1314
                     /Extension="AAAGGCCGGCCAGCG"
                     /Tm_(binding_region)="59.7"
                     /Tm_(with_extension)="78.8"
                     /%GC_(binding_region)="61.1"
                     /%GC_(with_extension)="66.7"
                     /Hairpin_Tm_(with_extension)="58.1"
                     /Self_Dimer_Tm_(with_extension)="45.1"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1 F) - pCS2+8 (digested by AsiSI, XbaI) (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1 F) - pCS2+8 (digested by AsiSI, XbaI) (<a
                     href=""urn:local:.:co-je2uw6b"">cyto roGFP2-Orp1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1 F) - pCS2+8 (digested by AsiSI, XbaI)
                     (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="88.89%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1 F) - pCS2+8 (digested by AsiSI, XbaI) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="cyto roGFP2-Orp1 (1 F) - pCS2+8 (digested by
                     AsiSI, XbaI)"
     primer_bind     complement(5382..5401)
                     /Sequence="TTACTTGTACAGCTCGTCCA"
                     /created_by="primer3"
                     /Product_Size=1137
                     /Extension="CGACTCACTATAGTTCTAG"
                     /Tm_(binding_region)="56.2"
                     /Tm_(with_extension)="62.5"
                     /%GC_(binding_region)="45.0"
                     /%GC_(with_extension)="43.6"
                     /Hairpin_Tm_(with_extension)="38.3"
                     /Self_Dimer_Tm_(with_extension)="9.2"
                     /Pair_Dimer_Tm_(binding_region)="1.5"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="85.00%"
                     /Primary_Match="85.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="85.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="85.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="85.00%; Adjusted-Interval-0:0/0; cyto
                     Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8 (digested by
                     AsiSI, XbaI) Fragment 1 (<a
                     href=""urn:local:.:cf-je2uv8b"">cyto Grx1-roGFP2</a>)"
                     /label="cyto Grx1-roGFP2 (1137 R) - H2B Cerulean in pCS2+8
                     (digested by AsiSI, XbaI) Fragment 1"
     CDS             5504..5992
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="99.4"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="HYR1"
     primer_bind     complement(5971..5995)
                     /Sequence="CTATTCCACCTCTTTCAAAAGTTCT"
                     /created_by="primer3"
                     /Product_Size=1314
                     /Extension="CGACTCACTATAGTTCTAG"
                     /Tm_(binding_region)="57.2"
                     /Tm_(with_extension)="61.5"
                     /%GC_(binding_region)="36.0"
                     /%GC_(with_extension)="38.6"
                     /Hairpin_Tm_(with_extension)="51.4"
                     /Self_Dimer_Tm_(with_extension)="20.8"
                     /Pair_Dimer_Tm_(binding_region)="None"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1314 R) - pCS2+8 (digested by AsiSI, XbaI)
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1314 R) - pCS2+8 (digested by AsiSI, XbaI)
                     (<a href=""urn:local:.:co-je2uw6b"">cyto roGFP2-Orp1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; cyto
                     roGFP2-Orp1 (1314 R) - pCS2+8 (digested by AsiSI, XbaI)
                     (cyto-roGFP2-Orp1-pCS2+8)"
                     /label="cyto roGFP2-Orp1 (1314 R) - pCS2+8 (digested by
                     AsiSI, XbaI)"
     ligation        5996..6014
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Ligation
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Ligation (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="Ligation"
     primer_bind     complement(6000..6019)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="4.4"
                     /Tm="50.3"
                     /created_by="primer3"
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Mismatch_Positions="==================GG"
                     /Mismatches=2
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T7
                     promoter (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="T7 promoter"
     promoter        complement(6002..6019)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.7"
                     /fragment="True"
                     /other="promoter"
                     /label="T7 promoter (fragment)"
     primer_bind     complement(6002..6018)
                     /%GC="35.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="44.2"
                     /created_by="primer3"
                     /Sequence="ATTAACCCTCACTAAAG"
                     /Mismatch_Positions="=T==ACC=======A=="
                     /Mismatches=5
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="T3 promoter (17bp)"
     polyA_signal    complement(6024..6158)
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /label="SV40 poly(A) signal"
     polyA_signal    6024..6158
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="SV40 poly(A) signal"
     polyA_signal    6024..6158
                     /note="SV40 polyadenylation signal"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="RFP_mChilada_LCK in pCS2_8"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:8x-etrca7i"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:3n-ev0i2rr"">mChilada_LCK Lv
                     polyubiquitinC</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:5w-eylipru"">H2B Cerulean plasmid-
                     Lv_polyubiquitinC</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40 poly(A) signal (<a
                     href=""urn:local:.:4z-eylieh4"">H2B Cerulean plasmid
                     (backbone unknown) - pabp in pUC-GW-Amp</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     poly(A) signal (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6048..6067)
                     /Tm=54
                     /Sequence="GTGGTTTGTCCAAACTCATC"
                     /Description="SV40 polyA terminator, reverse primer"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; EBV-rev
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EBV-rev (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     EBV-rev (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="EBV-rev"
     primer_bind     6102..6121
                     /Tm=51
                     /Sequence="GAAATTTGTGATGCTATTGC"
                     /Description="SV40 polyA, reverse primer"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40pA-R
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40pA-R (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SV40pA-R (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="SV40pA-R"
     protein_bind    6182..6215
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /label="lox2272"
     protein_bind    complement(6182..6215)
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GGATACTT). <i>lox2272</i> sites are compatible
                     with each other, but incompatible with <i>loxP</i> or
                     <i>loxN</i> sites (<a
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/9714735"">Lee
                     and Saito, 1988</a>)."
                     /modified_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LoxP/Cre Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lox2272
                     (<a href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="82.35%"
                     /X-Primary_Match="82.35%; Adjusted-Interval-0:0/0; lox2272
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="82.35%; Adjusted-Interval-0:0/0;
                     loxP (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lox2272
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="94.12%; Adjusted-Interval-0:0/0;
                     lox2272 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0;
                     lox2272 (<a href=""urn:local:.:de-je2v13m"">1-orp_01</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0;
                     lox2272 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0; loxP
                     (<a href=""urn:local:.:de-je2v13m"">1-orp_01</a>)"
                     /Alternative_Match="82.35%; Adjusted-Interval-0:0/0; loxP
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="lox2272"
     primer_bind     complement(6216..6249)
                     /Tm=59
                     /Sequence="CTTAAAACAATAATTTAATTAATTTCCCCCGCGG"
                     /Description="<html><body></body></html>"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a href=""urn:local:.:50-es1dlm7"">PhiC 31
                     Minos ITR MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a href=""urn:local:.:13-esbocyl"">LoxP/Cre
                     Minos ITR MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     MiMIC_3'_rev (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="MiMIC_3'_rev"
     repeat_region   complement(6267..6519)
                     /note="derived from Minos transposon"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:50-es1dlm7"">PhiC 31 Minos ITR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="derived from Minos transposon"
     repeat_region   6267..6519
                     /note="derived from Minos transposon"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:50-es1dlm7"">PhiC 31 Minos ITR
                     MCS</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:13-esbocyl"">LoxP/Cre Minos ITR
                     MCS</a>)"
                     /X-Transferred_From="Lv_polyubiquitin_H2B_mNeonGreen-pHEY1
                     "
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; derived
                     from Minos transposon (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="99.61%; Adjusted-Interval-0:0/0;
                     derived from Minos transposon (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="derived from Minos transposon"
     misc_feature    6402..6424
                     /X-Transferred_From="MiMIC_featured"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC_31_Minos_ITR_MCS in pTwist Amp
                     High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:4k-es16m6n"">MiMIC_featured</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     gRNA_Target (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="gRNA_Target"
     CDS             6610..6653
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="25.3"
                     /fragment="True"
                     /other="CDS"
                     /label="lacZα (fragment)"
     primer_bind     complement(6610..6627)
                     /Tm=56
                     /Sequence="TGTAAAACGACGGCCAGT"
                     /Description="In lacZ gene. Also called M13-F20 or M13
                     (-21) Forward"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Forward (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Forward (<a href=""urn:local:.:5j-etqqqce"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Forward (<a href=""urn:local:.:ni-etrlssm"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 Forward (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13 Forward"
     primer_bind     complement(6610..6626)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR
                     MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:5j-etqqqce"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13 fwd (<a href=""urn:local:.:ni-etrlssm"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 fwd
                     (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR
                     MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     fwd (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 fwd
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13 fwd"
     primer_R        complement(6611..6626)
                     /created_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PhiC 31 Minos ITR MCS Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13F
                     (<ahref=""urn:local:.:h-f47bj6f"">PhiC 31 Minos ITR MCS
                     Copy</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13F
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13F (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13F"
     misc_feature_   complement(6611..6626)
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_forward (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_forward (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="m13_forward"
     primer_bind     complement(6619..6641)
                     /Tm=61
                     /Sequence="CCCAGTCACGACGTTGTAAAACG"
                     /Description="In lacZ gene"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a href=""urn:local:.:8o-etrc9l3"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a href=""urn:local:.:5j-etqqqce"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a href=""urn:local:.:ni-etrlssm"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy -
                     L.variegatus_polyubiquitinC H2B Cerulean</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     Forward (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a href=""urn:local:.:8o-etrc9l3"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Forward (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     Forward (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13/pUC Forward"
     primer_bind     complement(6630..6653)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC-GW-Amp.dna"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13F(-47) (<a
                     href=""urn:local:.:1r-euq9nvm"">pUC-GW-Amp.dna</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13F(-47) (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13F(-47) (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13F(-47) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="M13F(-47)"
     promoter        6740..6844
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     misc_feature_   complement(6740..6844)
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="H2B_GFP_mAbbsynth in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:58-epr10bc"">H2B_GFP_mAbbsynth in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:5o-epr10cb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:h-hh7co6e"">Lifeact-mCherry in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a href=""urn:local:.:1j-he1rucf"">Minos
                     Lox Lvpolyubi LCK mNeongreen</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:g-hgwqs99"">pCS2FA-transposase</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a href=""urn:local:.:n-eurzkb4"">Pictus
                     Nanos 5' and 3' UTR MCS</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpR_promoter (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="AmpR_promoter"
     protein_bind    6781..6798
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /X-Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /X-Transferred_Similarity="88.24%"
                     /X-Primary_Match="88.24%; Adjusted-Interval-0:0/0; lac
                     operator (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /X-Alternative_Match="88.24%; Adjusted-Interval-0:0/0; lac
                     operator (cyto-roGFP2-Orp1-pCS2+8)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="77.78%"
                     /Primary_Match="77.78%; Adjusted-Interval-0:0/0; lac
                     operator (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="lac operator"
     primer_bind     6801..6817
                     /%GC="35.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="44.2"
                     /created_by="primer3"
                     /Sequence="ATTAACCCTCACTAAAG"
                     /Mismatch_Positions="=T=======C=CT==A="
                     /Mismatches=5
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-Grx1-roGFP2-pCS2+8  "
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (cyto-Grx1-roGFP2-pCS2+8  )"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (<a
                     href=""urn:local:.:c0-je2us6k"">cyto-Grx1-roGFP2-pCS2+8 
                     </a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; T3
                     promoter (17bp) (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="T3 promoter (17bp)"
     CDS             6845..7705
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
     misc_feature    6845..7705
                     /note="/application_notes=Allows E. coli to be resistant
                     to ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=39ee7c4ce93d4075b0df0df5df6b9861"
                     /X-Transferred_From="Minos Lox H2B Cerulean Lv
                     polyubiquitinC"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a href=""urn:local:.:t-fgi9koc"">Minos Lox
                     H2B Cerulean Lv polyubiquitinC</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:g-gkc2nm4"">hCas9-T2A-H2B
                     mCitrine</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:o-hfwuxxr"">EMTB-mCherry</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a href=""urn:local:.:c-fkl7ij5"">Piggybac
                     Transposase</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a
                     href=""urn:local:.:h-h1rgjqn"">pSFFV_mNG2(11)1-10
                     (reversed)</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a href=""urn:local:.:c-frx48cj"">Tol2
                     Transposase</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a
                     href=""urn:local:.:i-he1qclb"">memb_CER_JPC_05
                     (reversed)</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     Ampicillin (<a href=""urn:local:.:1b-fqqgbta"">HyBPase
                     tranposase</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a href=""urn:local:.:1j-he1rucf"">Minos Lox
                     Lvpolyubi LCK mNeongreen</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a href=""urn:local:.:t-fgi9koc"">Minos Lox
                     H2B Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:1n-hj6jtu7"">Lifeact-mhYFP</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:28-hj6k5n3"">Lifeact-LSSmOrange</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:1g-hhr5igo"">Lifeact-mTFP1</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:h-hh7co6e"">Lifeact-mCherry in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:g-gkc2nm4"">hCas9-T2A-H2B
                     mCitrine</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:2q-hj6khyv"">H2B-Electra2</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:14-hj6jb5q"">Lifeact-mRuby</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     AmpiciIIin (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="AmpiciIIin"
     misc_feature_   complement(6845..7705)
                     /X-Transferred_From="H2B_GFP_mAbbsynth in pCS2_8"
                     /X-Transferred_Similarity="99.88%"
                     /X-Primary_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:58-epr10bc"">H2B_GFP_mAbbsynth in
                     pCS2_8</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a
                     href=""urn:local:.:5o-epr10cb"">YFP_mAbbsynth_LCKin
                     pCS2_8</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK
                     in pCS2_8</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a href=""urn:local:.:n-eurzkb4"">Pictus Nanos 5'
                     and 3' UTR MCS</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a href=""urn:local:.:o-hfwuxxr"">EMTB-mCherry</a>)"
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ampR
                     (<ahref=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCK
                     mCherry</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:58-epr10bc"">H2B_GFP_mAbbsynth in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:5o-epr10cb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:h-hh7co6e"">Lifeact-mCherry in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a
                     href=""urn:local:.:g-hgwqs99"">pCS2FA-transposase</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:n-eurzkb4"">Pictus Nanos 5' and
                     3'UTR MCS</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; ampR
                     (<a href=""urn:local:.:o-hfwuxxr"">EMTB-mCherry</a>)"
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; ampR
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="99.88%; Adjusted-Interval-0:0/0;
                     ampR (<a
                     href=""urn:local:.:39-j3rukow"">Lv_polyubiquitin_H2B_mNeon
                     Green-pHEY1</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ampR (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="ampR"
     primer_bind     complement(7063..7082)
                     /Tm=58
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /Description="Ampicillin resistance gene, reverse primer"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="cyto-roGFP2-Orp1-pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Amp-R
                     (<a
                     href=""urn:local:.:c4-je2us6k"">cyto-roGFP2-Orp1-pCS2+8</a
                     >)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (cyto-roGFP2-Orp1-pCS2+8)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="Amp-R"
     attL1           7730..7826
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL1
                     (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:2e-ggvr0c6"">Minos_lox_LvEndo16_LCKmChe
                     rry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a href=""urn:local:.:t-fgi9koc"">Minos Lox H2B
                     Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL1
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attL1 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
     attL1           7730..7826
     attP1           7731..7930
                     /X-Transferred_From="Minos Lox H2B Cerulean Lv
                     polyubiquitinC"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP1
                     (<a href=""urn:local:.:t-fgi9koc"">Minos Lox H2B Cerulean
                     Lv polyubiquitinC</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP1
                     (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a href=""urn:local:.:1j-he1rucf"">Minos Lox
                     Lvpolyubi LCK mNeongreen</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a href=""urn:local:.:t-fgi9koc"">Minos Lox H2B
                     Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attP1
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attP1 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
     attP1           7731..7930
     protein_bind    7805..7829
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /label="attB1"
     attB1           7805..7829
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:1/-3;
                     attB1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:1/-3;
                     attB1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:1/-3;
                     attB1 (<a
                     href=""urn:local:.:h-j3ru7x7"">Lv_polyubiquitin_H2B_CFP</a
                     >)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB1
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attB1 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
     attR1           7805..7930
                     /note="Derived using Geneious Prime 2023.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Minos_lox_Lvpolyubi_LCKmCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1
                     (<a
                     href=""urn:local:.:3q-ggvrf2a"">Minos_lox_Lvpolyubi_LCKmCh
                     erry</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a href=""urn:local:.:t-fgi9koc"">Minos Lox H2B
                     Cerulean Lv polyubiquitinC</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a
                     href=""urn:local:.:h-hgsawk1"">Min_LvPoly_mNgLCK_A_01</a>)
                     "
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     attR1 (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
     misc_feature_   complement(7805..7829)
                     /X-Transferred_From="Minos_Lox_ITR_MCS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB1
                     (<a
                     href=""urn:local:.:46-j3rwkml"">Minos_Lox_ITR_MCS</a>)"
                     /note="Derived using Geneious Prime 2025.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LvPolyubiquitinC-Grx1-roGFP2-lox-minos"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attB1
                     (<a
                     href=""urn:local:.:b7-je2uquk"">LvPolyubiquitinC-Grx1-roGF
                     P2-lox-minos</a>)"
                     /label="attB1"
     attB1           7805..7829
     attR1           7805..7930
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttccagc
      661 gacatatgcc atacgccgaa ctgaactcca cctaatgtct tggagattgt cggatagcgc
      721 ggaataggat tgtgccctct gagcggataa caatttcaca caggaaacag ctatgaccat
      781 gacacatgca tccaccatcg cagacttatc atcactgagc ctccacctag cctcattctc
      841 tatgctagct taagatattt ttcgttttct acgagcccca accactatta attcgaacag
      901 catgtttttt ttgcagtgcg caatgtttaa cacactatat tatcaatact actaaagata
      961 acacatacca atgcatttcg tctcaaagag aattttattc tcttcacgac gaaaaaaaaa
     1021 gttttgctct atttccaaca acaacaaaaa tatgagtaat ttattcaaac ggtttgctta
     1081 agagataaga aaaaagtgac cactattaat tcgaacgcgg cgtaagctac cttaatctca
     1141 agaagagcaa aacaaaagca actaatgtaa cggataactt cgtatagcat acattatacg
     1201 aagttataac catatgtgac gtctacggat ccgcatgcac tagtcctcac aacatttctt
     1261 cccttttttg cgggagttgg ggcaaataat cctgacaatg tccaatgatt attttaaaaa
     1321 gtgctatgta caatcgcttt gtatttgtat aattgttata aaaaatgcat tgtatgtatt
     1381 tatatttaca tacataatta ttctacaaag tgtattgtac aatcacttta tatcaacttt
     1441 agatgtatcg tgttattagt gtcaaatctg actgcgtgca tactgattcg atgccaaaag
     1501 tggattaatg cctccttgtg ccccgcgtgt ctcttggcaa ctagagcatt attgtagaat
     1561 attggggcac ctaaaataca ctgacaagca atcatccaaa aatatctaac aaaatactaa
     1621 acaataacag gacggtcatt tttctgatat ctaaaacaag tttttgttga agggacattg
     1681 tgcaagactt tcaaaaactt attaagaatg ttgaaccgtt tacttttttt attttaacac
     1741 gtgatactat agtatacata acgattgggc gaaaaaatac cgtgacagtc aatcaaacaa
     1801 tctaaattcc cactttgcag aatcactttt gtgattccct tgtatcttca acaagatata
     1861 gatagatgga tagcatggaa gtttttgttt tttacttaga aatggtaaaa cgatgttttt
     1921 tccccagggt cttttccccc tatgtaaatt atgtttcatt ctttcaataa aaaaaaacac
     1981 aaactgaaaa aaaacttata agcaaaaaaa aaggcgtaaa tattttttta tgaactctgt
     2041 taaacggaaa caacaacaac acagtcgcca atatatacct cccgcgacca gtgatcattc
     2101 cagaaaatgt tttgggccga aattatattg cataattccg aaattgcgag tcatcatcgt
     2161 ctgcaatatc tggcgcgggt taatatgacg tcatgtgcca tagatacaca atctatcttt
     2221 attacccaat agaaaaggat gaaatagtcc atgtgacaca gactgaccaa tcgcaagtca
     2281 acacaaagca ccatataaaa cacacgcaag cgtcgcagtg tcaataaatt gcatgagact
     2341 tccgcagagg ttgcactcga gaagaactcg tccatttttt tccagaaaaa ctctaatttc
     2401 aaagtaagta gacgaggttt tcacattatt ttaaggcagt tatttcaatg aagatgcagt
     2461 ttgtaaatgt ttgatttaaa aaaagcactt ttgaagaaga atgatgatga agaacataaa
     2521 ttgtcaacga ccacatcgtc gtcattattc gtgtgctttt tttttgttgt aatttatgtt
     2581 tggctttcca aatgaaggca atataggaac attaacagca tgaacaaagt ctcatattta
     2641 gtttgattga aagttcaaat tatagggcgt agaaatcatt gaaattgtag ggtggatacc
     2701 gtggattatt gcgtcgtgtg tgtgcatgat aagatcgtct gactcagacg tcgcgtcttt
     2761 tgttgtgtgg tgtgtgtggt atggatgatg atcaattgtg atgtttggtg gggattttca
     2821 tgaacgctga attaaaagac aatgagtgga aatggccatg ccaatttctg gtccatggat
     2881 tgtgtgaatg caatgacaat ttcattcaat ttttgtctgg gctgccatga tgaacatgat
     2941 gaagtctgaa ctgaagtgtt gtgattgaaa tgacaaagaa tttttgtcat gagaaagaat
     3001 gaggcatgta cggtacggta acttaaaacg ttgatagcta ggtgtagatt ttctagatac
     3061 gtttggcagt aggcctagat ctagaggcac ttggccaatc atgaatagtc tatgggagac
     3121 aatttcccat attgaagact tgctttgcaa aagaaacgac accaacaaag cgtaattttt
     3181 tccaccccaa attttttcgc gaaggtcaga acagaagccc atggcaaagg cctgttttgt
     3241 atgtgcatgg ccatggatga aaaaaatata aatacaaaag ttggtgaatt tttaaagatg
     3301 aaaaggggta atttttcatg aggtatctgc ttaggcccta tcacattttt atttgctgcc
     3361 aaggtaattc ttttgctgca tgtgtaaaaa aaggaaattg gtctcccaaa ctgggagtgg
     3421 agactgtctc tgaaattgga tacccaagtt ctttaatctt tacaaaattc tgatattgaa
     3481 aataatctct ctcatgggag atgttctccc atattggctg tgcaccagtt ggtcggtgtg
     3541 caaagtctag acaatacgta tccaggcact atcgcctatg aataatgcaa tagcagtgca
     3601 gttcacaacg gcctctctcc cgatcagcgc agatgttggg gaactaattt caacttttgt
     3661 cgtaatttat gtcaaataac tgacagtcgt aacttaagac cctaatcagc taatgttaat
     3721 ccttgacctg aattgcaggc atctgaaacg gctgtaatcc agcttcgatc ttaatgtcat
     3781 agttcaaatt ctcagtagat ttaactgagc gtggtgcgtg tttggccatt gccatagttt
     3841 ttatatggac tgaagtcaac gaccaaaaga tctgctcaag gcagcccaac tgcattagca
     3901 agattttggt tgctattcag tcaatatcag aactacggca atggacgagt gcggcgatgt
     3961 caacctacat gtaactgaga attcaaacta tgacattgag aacgaagcca aaatacagca
     4021 aatattgatg tggttgaaac ttgggtcaag gatcaaatgg acaatatttg gcaaaaaaga
     4081 taaattttga ggtgaaattg tttcctgaca ttgccatgtt ggttggtgtg aaactgcagc
     4141 gcaagacaat gaaggaagag actttataga gtaagctgcc ccgaatttga catgttttga
     4201 accatttttt gttcttaagt cttttcacat aatgaaaatt gcattaaaat gatcaatgtt
     4261 ggttgaatat atttagaatt gcaatgttga atttctccat tttgaatatt tcataaaaaa
     4321 tctgcactgt accaccatgt tttgctcccg ccacagattt cttaatttta gagcatttta
     4381 tggctacctc atttcttaaa tctgagcaaa gctaccgcat gttattgaaa tatggcacag
     4441 attcgcattt ttaaagggga ttttggtgtt caagtcgtga cttgcatgtg gtgggtgata
     4501 atgcctgtgt atgtcaatgc atgtggttcg tgttgtgtgg attgactgag ctgacttgtg
     4561 gaataagttt gccatgttga attccaggca tgctactctg aataaaggtc tttagaaaaa
     4621 cagattccaa taatgttttc atttttttgt caattttcta caggtcagtg cgatcgcatc
     4681 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4741 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4801 cggcaagctg accctgaagt tcatctccac caccggcaag ctgcccgtgc cctggcccac
     4861 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     4921 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4981 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5041 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5101 caagctggag tacaactaca actgccacaa cgtctatatc atggccgaca agcagaagaa
     5161 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5221 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5281 ctacctgagc acctgctccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5341 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagac
     5401 tagtggtggt tcaggtggtg gtggttcagg tggtggtggt tcaggtggag gaggatcagg
     5461 aggaggagga tcaggaggag gaggatcagg aggagaattc gatatctcag aattctataa
     5521 gctagcacct gttgacaaga aaggccaacc attccccttc gaccaattaa agggaaaagt
     5581 ggtgcttatc gttaatgttg cctccaaatg tggattcact cctcaataca aagaactaga
     5641 ggccttgtac aaacgttata aggacgaagg atttaccatc atcgggttcc catgcaacca
     5701 gtttggccac caagaacctg gctctgatga agaaattgcc cagttctgcc aactgaacta
     5761 tggcgtgact ttccccatta tgaaaaaaat tgacgttaat ggtggcaatg aggaccctgt
     5821 ttacaagttt ttgaagagcc aaaaatccgg tatgttgggc ttgagaggta tcaaatggaa
     5881 ttttgaaaaa ttcttagtcg ataaaaaggg taaagtgtac gaaagatact cttcactaac
     5941 caaaccttct tcgttgtccg aaaccatcga agaacttttg aaagaggtgg aatagctaga
     6001 actatagtga gtcgtattac gtagatccag acatgataag atacattgat gagtttggac
     6061 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6121 ctttatttgt aaccattata agctgcaata aacaagttgg ccgccttcac gcgtggttac
     6181 cataacttcg tataggatac tttatacgaa gttatccgcg ggggaaatta attaaattat
     6241 tgttttaagt atgatagtaa atcacattac gccgcgttcg aattaatagt ggtcactttt
     6301 ttcttatctc ttaagcaaac cgtttgaata aattactcat atttttgttg ttgttggaaa
     6361 tagagcaaaa cttttttttt cgtcgtgaag agaataaaat tctctttgag acgaaatgca
     6421 ttggtatgtg ttatctttag tagtattgat aatatagtgt gttaaacatt gcgcactgca
     6481 aaaaaacatg ctgttcgaat taatagtggt tggggctcgt agaagctgct aacgtgcttg
     6541 gcataattta tagaagcagg tcaggcggaa tggcacttcc ttattccaga tgcgtgcgga
     6601 ttatgcatga ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgcttgtgg
     6661 tggatcacag ttattggaga cttccttggc cgtacgatgg gttttgtttc aggtatctgg
     6721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6841 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6901 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6961 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7021 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7081 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7141 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7201 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7261 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7321 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7381 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7441 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7501 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7561 ctctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7621 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7681 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7741 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttg aattgccatt
     7801 atcaacaagt ttgtacaaaa aagcaggctc cgaattggta tcacgaggac gatttgaagc
     7861 cccctaccca tttacgctga cttgacggga cggcggcttt gttgaataga gcgtcagacc
     7921 ccgtagaaaa gatcaaagga tcttc
//
