LOCUS       pLN053        6404 bp DNA     circular SYN 07-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   111111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6404
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    299..358
                     /standard_name="rrnBT1T2"
                     /label=rrnBT1T2
     primer_bind     333..358
                     /label=41SEQ-3F
     primer_bind     complement(426..480)
                     /label=LNS053-2R
     primer_bind     459..492
                     /label=LNS053-1F
     misc_feature    470..2155
                     /label=Feature 39
     gene            join(576..1915,1916..2027)
                     /gene="rsaFb"
                     /locus_tag="CCNA_01379"
                     /label=rsaFb
                     /db_xref="GeneID:7331837"
     CDS             join(576..1915,1916..2027)
                     /codon_start=1
                     /gene="rsaFb"
                     /locus_tag="CCNA_01379"
                     /product="type I secretion outer membrane protein rsaFb"
                     /label=rsaFb
                     /note="CC_1318"
                     /db_xref="GeneID:7331837"
                     /experiment="EXISTENCE:LC-MS"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002516752.2"
                     /translation="MLMSNRRRAGLLAAACSAGLLAGSFSIANAETLADAIALAYQTNP
                     TLQQQRASARITDEGVVQAKTGFRPTVGATADITGANTNPASAGADVKLSGSSAALSII
                     QPLYTGGRASAGVSAAEADVLSAREGLRAVEQGVLVSVVQAYVDVRRDQERLRIAKENV
                     AVLQRQLEESNARFDVGEITRTDVAQSQARLASAKASLSGAQAQLEVSRASYAAVVGQT
                     PGELAPEPSLAGLLPASVDQAFEAAQTNNPGVTSAKFNEEAAAARVAVAKAGYRPTVSA
                     RASLGYDATRRSGVGSQFDDYSRTVSGAITASIPLFAGGLTTSQVRAANERESAARSAV
                     EGAKRTAIQQVSNAWSNLLASRAGLVANEEQVRATRIAFEGVRQEQQVGLRTTLDVLNA
                     QLELSNAELALVSARRDEYLASAVVLQAMGQLEVTKLASNTAAYDPKTSYNRVTRGVGW
                     VPWEPVVQAIDSVGAASTKPPSGSK"
     primer_bind     complement(1894..1926)
                     /label=LNS053-1R
     primer_bind     1905..1936
                     /label=LNS053-2F
     primer_bind     complement(1926..1936)
                     /label=LNS053-2F
     primer_bind     complement(2135..2166)
                     /label=LNS-1R
     primer_bind     2146..2176
                     /label=LNS-2F
     misc_feature    2156..2863
                     /standard_name="mCherry"
                     /label=mCherry
     primer_bind     2156..2177
                     /label=WZ0330_(mch-popz)_forward
     primer_bind     2156..2177
                     /label=WZ0028_(mcherry)_forward
     primer_bind     2156..2177
                     /label=WZ0027_(pxchyc-6)_forward
     primer_bind     complement(2156..2170)
                     /label=WZ0329_(pvan)_reverse
     primer_bind     complement(2281..2299)
                     /label=41SEQ-1R
     primer_bind     complement(2842..2878)
                     /label=WZ0010_(PCDF-YFP)_reverse
     primer_bind     2849..2878
                     /label=WZ0011_(HRSAT)_(PODJ)_forward
     primer_bind     complement(2854..2878)
                     /label=WZ0029_(mcherry)_reverse
     primer_bind     complement(2858..2897)
                     /label=WZ0365_(pwz0139)_reverse
     primer_bind     2864..2900
                     /label=WZ0366_(sfgfp)_forward
     primer_bind     2864..2878
                     /label=WZ0030_(popz)_forward
     misc_feature    2879..3592
                     /label=sfgfp
     primer_bind     2879..2907
                     /label=WZ0361_(gs-linker)_(pwz0113)_forward
     primer_bind     2879..2900
                     /label=WZ0286_(sfGFP)_forward
     primer_bind     complement(2879..2890)
                     /label=WZ0285_(p0102)_reverse
     primer_bind     3483..3501
                     /label=41SEQ-2F
     primer_bind     complement(3566..3623)
                     /label=WZ0367_(sfgfp)_(gs)_reverse
     primer_bind     complement(3566..3592)
                     /label=WZ0287_(sfGFP)_reverse
     primer_bind     3578..3592
                     /label=WZ0284_(p0102)_forward
     primer_bind     3593..3634
                     /label=WZ0364_(pwz0139)_forward
     primer_bind     complement(3593..3607)
                     /label=WZ0080_(spmx)_reverse
     primer_bind     3593..3607
                     /label=WZ0081_(ggsgs)_(yfp)_forward
     primer_bind     3593..3607
                     /label=WSC10344_(GSlinker)_(yfp)_forward
     misc_feature    3593..3607
                     /label=gly_ser_linker
     CDS             3593..3607
                     /label=GFPuv
     misc_feature    3593..3607
                     /label=short gly/ser linker
     misc_feature    3593..3607
                     /label=long gly/ser linker
     primer_bind     complement(3593..3605)
                     /label=WSC10343_(podj-2)_reverse
     misc_feature    3608..4141
                     /label=popz
     primer_bind     3608..3638
                     /label=WZ0030_(popz)_forward
     primer_bind     complement(3608..3623)
                     /label=WZ0029_(mcherry)_reverse
     primer_bind     complement(4122..4141)
                     /label=WZ0331_(mch-popz)_reverse
     primer_bind     complement(4124..4152)
                     /label=z-1R
     primer_bind     complement(4124..4141)
                     /label=WZ0031_(popz)_reverse
     primer_bind     4127..4141
                     /label=WZ0328_(pvan)_forward
     primer_bind     4131..4180
                     /label=z-2F
     primer_bind     complement(4246..4265)
                     /label=pXYFPN-2-R (WTP142)
     primer_bind     complement(4333..4351)
                     /label=43SEQ-1R
     misc_feature    4455..5069
                     /standard_name="rep"
                     /label=rep
     misc_feature    5467..6282
                     /standard_name="nptI"
                     /label=nptI
                     /label="Kan"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttac cgtcgcaacg
      481 tttgcgtgaa aaaagcttaa ctggcgacga ctggcggagc gacttacacg cgatcatatt
      541 gtggccggcg cgagcttcgc gtgattgggg atagaatgtt gatgtcgaac cgtcgacggg
      601 ccgggttgct ggccgccgct tgcagcgctg gtcttctggc cgggtccttc tccatcgcga
      661 acgccgagac tctcgccgat gcgatcgcgc tcgcctatca gaccaatccg acgctgcagc
      721 aacagcgcgc cagcgcccgc atcaccgacg aaggcgtggt gcaagccaag accggcttcc
      781 gcccgaccgt gggcgccacc gccgatatca cgggggcgaa caccaatccg gccagcgccg
      841 gcgcggatgt gaagctctct ggcagcagcg cggcgctttc gatcatccag ccgctctaca
      901 ccggcggtcg cgccagcgcg ggcgtcagcg ccgctgaagc cgacgtgctg tctgcgcggg
      961 aaggtcttcg cgcggtcgag cagggggtgc tggtcagcgt cgtccaggcc tatgtcgacg
     1021 tgcgccgaga ccaggaacgc ctgcgcatcg ccaaggaaaa cgtcgcggtt ctgcagcgcc
     1081 agctcgaaga atcgaacgct cgcttcgacg tgggtgagat cacccggacg gacgtcgccc
     1141 agtctcaggc gcgcttggct tcggccaagg ccagcctgtc gggcgcccag gcccagttgg
     1201 aagtcagccg cgcctcctac gctgcggtgg tcggtcaaac gcccggcgaa ctggctcccg
     1261 agccgagctt ggccggactg ctgcccgcca gcgtcgacca ggctttcgag gccgcccaga
     1321 cgaacaatcc tggcgtgacc tcggcgaagt tcaatgaaga ggcggccgcc gcgcgtgtcg
     1381 ccgtcgccaa ggcgggctat cgaccgacgg tctcggcgcg cgcttcgctg ggttacgatg
     1441 cgacgcgtcg ttctggcgtc ggcagccagt ttgacgacta ttcgcgcacc gtttcgggcg
     1501 cgatcacggc ctcgatcccg cttttcgccg gcggcttgac cacgtcgcag gtccgggcgg
     1561 ccaatgaacg tgagagcgct gcgcgcagcg ccgtcgaagg cgccaagcgc accgcgatcc
     1621 agcaagtctc gaacgcgtgg agcaatctgc tggcgtcgcg cgccggtctg gtcgccaacg
     1681 aggagcaggt tcgagcgacc cgcatcgctt tcgaaggcgt gcgtcaggag caacaggtcg
     1741 gcctgcggac gacgctggac gttctgaacg cccagctgga gctgtccaac gccgaactgg
     1801 cgctggtttc ggcccgtcgc gacgaatacc tggcttccgc cgtggtcttg caggccatgg
     1861 gccaactgga ggtgaccaag ctcgcctcca acaccgccgc ctatgacccg aaaacctcct
     1921 acaaccgcgt gacgcgcggc gtgggctggg tgccgtggga gccggtggtg caggcgatcg
     1981 acagcgtcgg cgcggcttcc accaagcccc cgagcggctc gaaataggtt tgatccgcgt
     2041 cgattccgcc caggcgcgga cttgcgcttg cttaacacgc gtaagtctgc accatcgcgg
     2101 ctaagcgcgc cccaaggcgc gcaaggattc gtacctcttt acgacggccc cgcacatggt
     2161 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     2221 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     2281 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     2341 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     2401 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     2461 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     2521 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     2581 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     2641 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     2701 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     2761 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     2821 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagcaccggt cggccaccat
     2881 gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac tggatggtga
     2941 tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa
     3001 actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc cgactctggt
     3061 aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata tgaagcagca
     3121 tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga tttcctttaa
     3181 ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa
     3241 ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg gccataagct
     3301 ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa aaaatggcat
     3361 taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc tggctgatca
     3421 ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca atcactatct
     3481 gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata tggttctgct
     3541 ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca aaggtggcag
     3601 tggtagcatg tccgatcagt ctcaagaacc tacaatggag gaaatcctcg cctccattcg
     3661 acgcatcatc tcggaggatg acgcgccggc ggagcctgcg gccgaagcgg cgcccccgcc
     3721 gccgccggaa cccgaacctg aaccggtgtc gttcgacgac gaggttctgg aattgacgga
     3781 tccgatcgcg cccgagcccg agctgccgcc gctggagact gtcggcgaca tcgacgtcta
     3841 ttcgccgccg gaacctgagt cggaaccggc ctacacgccg ccgccggcgg ctccggtgtt
     3901 tgatcgcgac gaagtcgccg agcagctggt cggcgtttcg gccgcttcgg ccgcggcgag
     3961 cgccttcggc agcctgagct cggccctgct gatgcccaag gacggtcgga cgctggaaga
     4021 cgtcgtacgc gagctgctgc gcccgctgct caaggagtgg ctggaccaga acctgccgcg
     4081 catcgtcgag accaaggttg aggaagaagt gcagcgtatc tctcggggac gcggcgccta
     4141 agccttaatt aatatgcatg gtaccttaag atctcgagct ccggagaatt cgaacgttac
     4201 gcgtaccggt gctagctgca gcccggggga tccactagtt ctagagcggc cattcactgg
     4261 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4321 cagcacatcc ccctttcgcc agcggtatca gctcactcaa aggcggtaat acggttatcc
     4381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4441 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5161 atccttttgg gggggggggg cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat
     5221 accaggcctg aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag
     5281 ctttgttgta ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg
     5341 cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac
     5401 aaagccgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa
     5461 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     5521 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     5581 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     5641 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     5701 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
     5761 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     5821 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     5881 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     5941 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     6001 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     6061 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     6121 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     6181 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     6241 tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     6301 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     6361 acatcagaga ttttgagaca caacgtggct ttcccccccc cccc
//
