LOCUS       pLN051        6711 bp DNA     circular SYN 11-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   111111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6711
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    299..358
                     /standard_name="rrnBT1T2"
                     /label=rrnBT1T2
     primer_bind     333..358
                     /label=41SEQ-3F
     primer_bind     complement(432..490)
                     /label=LNS051-2R
     primer_bind     471..498
                     /label=LNS051-1F
     misc_feature    481..4448
                     /label=Feature 10
     gene            481..1070
                     /locus_tag="CCNA_02127"
                     /label=CCNA_02127
                     /db_xref="GeneID:7330433"
     CDS             481..1070
                     /codon_start=1
                     /locus_tag="CCNA_02127"
                     /product="bifunctional lysylphosphatidylglycerol 
                     flippase/synthetase MprF"
                     /label=CCNA_02127
                     /note="CC_2047"
                     /db_xref="GeneID:7330433"
                     /experiment="EXISTENCE:LC-MS"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517500.1"
                     /translation="AAPTMMDELKSISDSWLSHHAGGEKSFSMGGFDPRYVAEFPVAVV
                     RSEGKIVAFATLWTTAAKTAFSMDLMRYSDEAPKNVMDYLFVELLQWGKDEGYSAFEFG
                     VAPLAGLQDRPLAPIMSRVGRLLYERGEEIYNFQGVRRYKDKYDPVWQPRYIAAPQKWA
                     IPFLLADIGLLSSGGVSGLAKRPKKLPEKAAT"
     primer_bind     962..980
                     /label=poseq-1F
     gene            complement(1067..1282)
                     /locus_tag="CCNA_02126"
                     /label=CCNA_02126
                     /db_xref="GeneID:7330432"
     CDS             complement(1067..1282)
                     /codon_start=1
                     /locus_tag="CCNA_02126"
                     /product="hypothetical protein"
                     /label=CCNA_02126
                     /note="CC_2046"
                     /db_xref="GeneID:7330432"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517499.1"
                     /translation="MDLPVTAAAAILFLILAVLFGWLGARPINILKGPRLIPWRPMMMI
                     CVVGLMLMLVHLVNLLGVQTGNQTRY"
     primer_bind     complement(1111..1129)
                     /label=poseq-1R
     gene            1491..4415
                     /gene="podJ"
                     /locus_tag="CCNA_02125"
                     /label=podJ
                     /db_xref="GeneID:7330431"
     CDS             1491..4415
                     /codon_start=1
                     /gene="podJ"
                     /locus_tag="CCNA_02125"
                     /product="polar development protein podJ"
                     /label=podJ
                     /note="CC_2045"
                     /db_xref="GeneID:7330431"
                     /experiment="EXISTENCE:LC-MS"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517498.1"
                     /translation="MTAASPWSVKGIDPKAREVAKDLARRSGMTLGEWLNRMIIEGDGQ
                     TADPRLAGDDVPNRAYLEIVKDDAPPRIEIAEHPADEVGRVALALDRLTQRIEAAEGRN
                     AAAITGIDHSVRDALTRLGASEREQIAVAARFEGAVDELKTEQARATERLRRIESEAAG
                     PRSAEALRALEGALGKVAGHLYEGEARTREAIATLEAKLNQQSSGDPSALVEAVVARLG
                     ERLEAAETRTSDALRELGASFQALDQRLGAVETANPATGVQEGLDSLAATLTQKMEAAR
                     LEMAAKLRESADGRFDRMERKLGEMAAHVQAAEQRSAQAIERMGREIVGVADAFNRRVH
                     AAESRNASAIEQVGGEVARIAASVEHKLNRADSVQAQALEKLGGEIARITEKLAERIGS
                     AERRNALAIDDVGEQVARVTERLNQRHERSSQELVDRIRQSEERTLRMLEEAREKIDSR
                     LSEAQRKLEAAPPSPPPAQAPAPVATAQRPVPPAASPFEDNYFSQAASFSTSEDEADAF
                     DAPPAPARSFEVAEFPAAEPEEPAFAHDDYAIADGFEPESPRYEVEPEVSDFAPAEPSR
                     PMSTRDIIEQARAAARAAAASEGKGGKAKSAKKEKASKASGSLFSGFGGFSTKKSKARL
                     GATVTTALVVFAAAGALGAGVGGLLLLNTDDGNNSPSRVAQAIAGRKADVEVNGPEADT
                     TPGAPRAAVALTTGKVVPAEVEAPAAPPTNEAKALFEDAVRKIESGDRSGVELLKRAAN
                     GGYPAAQFYLSKMYEGGKNGVKVDMAEARRWSERAANGGDPRAMHNLALYYFKGEGGPR
                     NSTTAASWFRKAADMGLVDSQFNLAQLYESGLGVSQNPAEAYKWYVIAGRAGDSTARGR
                     ATALRSQLTAEAQQTADRSALAFRPQTQVQTASLSSAAPAAANANLGVAQRVLSQLGYY
                     QGPRDGVSSPALRMAIAAYQRDQGLPPTGSVDAETLNRLSVYAR"
     primer_bind     1607..1625
                     /label=poseq-2F
     primer_bind     2082..2100
                     /label=Primer 1
     primer_bind     2246..2264
                     /label=poseq-3F
     primer_bind     complement(2856..2874)
                     /label=Primer 2
     primer_bind     2877..2895
                     /label=poseq-4F
     primer_bind     3482..3500
                     /label=poseq-5F
     primer_bind     4043..4067
                     /label=poseq-6F
     primer_bind     complement(4430..4459)
                     /label=LNS051-1R
     primer_bind     4439..4489
                     /label=LNS051-2F
     primer_bind     complement(4553..4572)
                     /label=pXYFPN-2-R (WTP142)
     primer_bind     complement(4640..4658)
                     /label=43SEQ-1R
     misc_feature    4762..5376
                     /standard_name="rep"
                     /label=rep
     misc_feature    5774..6589
                     /standard_name="nptI"
                     /label=nptI
                     /label="Kan"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 gtgcggcgcc gaccatgatg gacgagctga agtccatctc ggactcctgg ctttcgcatc
      541 acgccggcgg cgaaaagagc ttctcgatgg ggggctttga cccacgttat gtggccgagt
      601 tccccgtcgc ggttgttcgc agtgaaggca agatcgtcgc cttcgccacg ctgtggacga
      661 cggccgccaa gacggcgttc tccatggacc tgatgcgcta ctctgacgaa gcgcccaaga
      721 acgtcatgga ctatctgttc gtcgagctgc tgcagtgggg caaggacgag ggatacagcg
      781 ccttcgagtt cggcgtcgcg ccgctggcgg gtctgcagga tcgccctctg gccccgatca
      841 tgtcgcgcgt cggccggctg ctctacgagc gcggcgagga gatctataac ttccagggtg
      901 tgcgtcgcta caaggacaag tacgacccgg tctggcagcc gcgctacatt gccgcgcctc
      961 agaagtgggc gatcccgttc ctgctcgccg acatcgggct gctgtcgtcc ggtggggtct
     1021 ccggcctggc gaaacgaccc aagaagctcc cggagaaggc cgcgacctag tagcgcgtct
     1081 ggttacccgt ctggacgccg agcaggttca ccagatgcac cagcatcagc atcagtccga
     1141 ccacgcagat catcatcatc ggccgccaag ggatcagccg cgggcccttg agaatattga
     1201 tcggacgcgc gcccagccag ccgaagagca cggcgaggat caggaacagg atcgcggcgg
     1261 cggcggtgac gggcaggtcc atgcgcgctt ctaggcctgc gacaatccgc ctggcaaatg
     1321 gttaaccagt gttaaccctc ttgcttttga accgcgctac agagtgatgg gactctgtcc
     1381 tgattcctcc acagtgaatc gcacagccac tacatctggc gttttcgtcg cgtttacacc
     1441 ctaggaatcg gtgactagcg ttttccccag gtgcggggag atcgattcgc atgacggcgg
     1501 cttcgccatg gagcgttaag gggattgacc ccaaggcacg ggaggtcgcg aaagacctcg
     1561 cgcgtcgctc cggcatgact ttgggtgaat ggctgaaccg aatgatcatc gaaggcgatg
     1621 gtcagaccgc tgatccgcgt ctcgcggggg acgatgtccc caaccgcgcc tatctcgaga
     1681 ttgtcaaaga tgatgcgccg ccgcgcatcg agatcgccga gcatccggca gatgaggttg
     1741 gtcgcgtcgc gctggcgctg gatcgcctga cccaacgcat tgaagccgcc gaggggcgca
     1801 acgccgccgc catcaccggc atcgatcact ccgtccgtga cgccctgacg cgtctgggcg
     1861 catccgaacg tgagcagatc gccgtcgccg cccggttcga gggcgctgtc gacgaactga
     1921 agaccgagca ggctcgggcc accgaacgtc tgcgccggat cgagagcgag gcggcgggtc
     1981 cgcgctcggc cgaggcgttg cgcgcgcttg aaggcgcctt gggcaaggtc gccggacacc
     2041 tttacgaagg cgaggcgcgg acgcgcgagg cgatcgcgac cctggaagcc aagctgaacc
     2101 agcagagctc gggcgatccc agcgcgctgg tcgaggctgt ggtcgcgcga ctgggcgaac
     2161 gcctcgaagc cgccgaaacc cgcaccagcg acgcgctgcg cgaactgggc gcgtcgttcc
     2221 aggccctgga tcagcgcttg ggcgccgtcg agactgccaa tcccgccacg ggcgttcagg
     2281 agggcctgga cagcctggcc gccaccctca cgcagaagat ggaagccgct cgcctggaga
     2341 tggcggccaa gcttcgcgaa agcgccgacg gacgtttcga tcgcatggag cgcaagctcg
     2401 gcgagatggc ggcccacgtg caggcggccg aacagcgctc ggcccaggcc attgagcgta
     2461 tgggccgcga gattgtcggt gtcgctgatg cgttcaatcg ccgcgtccac gccgccgaga
     2521 gccgtaacgc cagcgctatc gagcaggtcg gtggcgaggt tgcgcggatc gccgccagcg
     2581 tcgagcacaa gctcaatcgc gccgatagcg ttcaagccca ggcccttgaa aagctgggcg
     2641 gcgagatcgc ccggatcacc gagaagctgg ccgagcggat cggcagcgcc gagcgacgca
     2701 acgccctggc catcgacgat gtcggcgaac aggtcgcccg cgtcacagag cgcctgaacc
     2761 aacgccacga acgttccagc caggaactgg tcgaccgcat ccgccagagc gaagagcgca
     2821 cgctccgcat gctggaagaa gcgcgcgaga agatcgacag ccgtctgtcc gaggcgcagc
     2881 ggaagctgga agctgcgccg ccgtcgccgc cgcccgcgca ggctccggcg ccggttgcga
     2941 ctgctcagcg tccggttccg ccggcggctt cgcccttcga ggacaactat ttcagccagg
     3001 ccgcctcgtt cagcacgtct gaggatgagg ctgacgcgtt tgatgcgccg cctgcgccgg
     3061 cccgtagctt cgaggtggcg gagttcccgg ctgcggagcc ggaagaaccg gccttcgcac
     3121 acgatgacta cgcgatcgcc gatggcttcg agccggaaag cccgcgctat gaagtcgagc
     3181 ctgaggtctc cgacttcgcg cccgccgagc cgtcgcgtcc gatgtcgacc cgcgatatca
     3241 tcgagcaggc ccgcgccgcc gcgcgcgccg ccgccgcctc ggaaggcaag ggcggcaagg
     3301 ccaagtcggc gaagaaggaa aaggcttcca aggccagcgg ctcgctgttc agcggctttg
     3361 gcggtttctc gaccaagaag tcgaaggcgc gcttgggcgc gaccgtgacg acggccctcg
     3421 ttgtcttcgc cgccgccggc gcgctcgggg ccggcgtggg cggcctgctg ctgctgaaca
     3481 ccgacgacgg caacaactcg cccagccgcg tcgcccaggc gatcgctggt cgcaaggccg
     3541 atgtcgaggt gaatggtccc gaggctgaca cgacccccgg cgcgccgcgc gccgccgtcg
     3601 cgctgacgac gggcaaggtc gtcccagccg aggtcgaggc ccctgcagcc ccgccgacca
     3661 acgaagccaa ggccctgttc gaggacgcgg tccgtaagat cgagtcgggg gaccgctcgg
     3721 gcgtcgagtt gctgaagcgc gccgccaatg gcggctaccc ggccgcgcag ttctacctct
     3781 cgaagatgta cgagggcggc aagaacggcg tgaaggtcga tatggccgaa gcgcgccgct
     3841 ggagtgagcg cgccgccaac ggtggcgatc cgcgggccat gcacaacctg gccctctact
     3901 acttcaaggg cgagggcggt ccgcgcaatt cgacgacggc cgccagctgg ttccgcaagg
     3961 ccgccgacat gggcctggtc gacagccagt tcaacctggc tcagctctac gaaagcggac
     4021 tgggcgtcag ccagaatccg gccgaagcct acaaatggta tgtgatcgcc ggccgcgctg
     4081 gggattcgac cgcgcggggc cgcgccacgg cgctgcgctc gcagctgacc gcagaggccc
     4141 agcagaccgc tgaccgctcg gccttggcct tccgtccgca gactcaggtc cagaccgcca
     4201 gtctgtcgtc cgctgcgccg gccgccgcaa acgccaacct tggcgttgcg cagcgtgtcc
     4261 tgtcgcagct gggctattac caggggccgc gcgacggtgt ctcgtcgccc gccctgcgca
     4321 tggcgatcgc ggcctatcag cgtgatcagg gtctgccccc caccggatcg gtggacgccg
     4381 agaccctcaa tcgcctgtcg gtctacgcgc gctaaacgcc tcgcgacctc gcgctttgcc
     4441 gggaaggggc cttaattaat atgcatggta ccttaagatc tcgagctccg gagaattcga
     4501 acgttacgcg taccggtgct agctgcagcc cgggggatcc actagttcta gagcggccat
     4561 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4621 cgccttgcag cacatccccc tttcgccagc ggtatcagct cactcaaagg cggtaatacg
     4681 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5461 cacctagatc cttttggggg gggggggcgc tgaggtctgc ctcgtgaaga aggtgttgct
     5521 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg
     5581 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa
     5641 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
     5701 attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat
     5761 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     5821 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     5881 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     5941 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     6001 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     6061 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     6121 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     6181 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     6241 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     6301 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     6361 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     6421 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     6481 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     6541 aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg
     6601 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     6661 caatgtaaca tcagagattt tgagacacaa cgtggctttc cccccccccc c
//
