LOCUS       pLN055        6168 bp DNA     circular SYN 18-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   111111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    299..358
                     /standard_name="rrnBT1T2"
                     /label=rrnBT1T2
     primer_bind     333..358
                     /label=41SEQ-3F
     primer_bind     complement(432..490)
                     /label=LNS051-2R
     primer_bind     471..498
                     /label=LNS051-1F
     misc_feature    481..3905
                     /label=Feature 10
     CDS             481..1070
                     /codon_start=1
                     /locus_tag="CCNA_02127"
                     /product="bifunctional lysylphosphatidylglycerol 
                     flippase/synthetase MprF"
                     /label=CCNA_02127
                     /note="CC_2047"
                     /db_xref="GeneID:7330433"
                     /experiment="EXISTENCE:LC-MS"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517500.1"
                     /translation="AAPTMMDELKSISDSWLSHHAGGEKSFSMGGFDPRYVAEFPVAVV
                     RSEGKIVAFATLWTTAAKTAFSMDLMRYSDEAPKNVMDYLFVELLQWGKDEGYSAFEFG
                     VAPLAGLQDRPLAPIMSRVGRLLYERGEEIYNFQGVRRYKDKYDPVWQPRYIAAPQKWA
                     IPFLLADIGLLSSGGVSGLAKRPKKLPEKAAT"
     gene            481..1070
                     /locus_tag="CCNA_02127"
                     /label=CCNA_02127
                     /db_xref="GeneID:7330433"
     primer_bind     962..980
                     /label=poseq-1F
     CDS             complement(1067..1282)
                     /codon_start=1
                     /locus_tag="CCNA_02126"
                     /product="hypothetical protein"
                     /label=CCNA_02126
                     /note="CC_2046"
                     /db_xref="GeneID:7330432"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517499.1"
                     /translation="MDLPVTAAAAILFLILAVLFGWLGARPINILKGPRLIPWRPMMMI
                     CVVGLMLMLVHLVNLLGVQTGNQTRY"
     gene            complement(1067..1282)
                     /locus_tag="CCNA_02126"
                     /label=CCNA_02126
                     /db_xref="GeneID:7330432"
     primer_bind     complement(1111..1129)
                     /label=poseq-1R
     CDS             1491..3872
                     /codon_start=1
                     /gene="podJ"
                     /locus_tag="CCNA_02125"
                     /product="polar development protein podJ"
                     /label=podJ
                     /note="CC_2045"
                     /db_xref="GeneID:7330431"
                     /experiment="EXISTENCE:LC-MS"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517498.1"
                     /translation="MTAASPWSVKGIDPKAREVAKDLARRSGMTLGEWLNRMIIEGDGQ
                     TADPRLAGDDVPNRAYLEIVKDDAPPRIEIAEHPADEVGRVALALDRLTQRIEAAEGRN
                     AAAITGIDHSVRDALTRLGASEREQIAVAARFEGAVDELKTEQARATERLRRIESEAAG
                     PRSAEALRALEGALGKVAGHLYEGEARTREAIATLEAKLNQQSSGDPSALVEAVVARLG
                     ERLEAAETRTSDALRELGASFQALDQRQELVDRIRQSEERTLRMLEEAREKIDSRLSEA
                     QRKLEAAPPSPPPAQAPAPVATAQRPVPPAASPFEDNYFSQAASFSTSEDEADAFDAPP
                     APARSFEVAEFPAAEPEEPAFAHDDYAIADGFEPESPRYEVEPEVSDFAPAEPSRPMST
                     RDIIEQARAAARAAAASEGKGGKAKSAKKEKASKASGSLFSGFGGFSTKKSKARLGATV
                     TTALVVFAAAGALGAGVGGLLLLNTDDGNNSPSRVAQAIAGRKADVEVNGPEADTTPGA
                     PRAAVALTTGKVVPAEVEAPAAPPTNEAKALFEDAVRKIESGDRSGVELLKRAANGGYP
                     AAQFYLSKMYEGGKNGVKVDMAEARRWSERAANGGDPRAMHNLALYYFKGEGGPRNSTT
                     AASWFRKAADMGLVDSQFNLAQLYESGLGVSQNPAEAYKWYVIAGRAGDSTARGRATAL
                     RSQLTAEAQQTADRSALAFRPQTQVQTASLSSAAPAAANANLGVAQRVLSQLGYYQGPR
                     DGVSSPALRMAIAAYQRDQGLPPTGSVDAETLNRLSVYAR"
     gene            1491..3872
                     /gene="podJ"
                     /locus_tag="CCNA_02125"
                     /label=podJ
                     /db_xref="GeneID:7330431"
     primer_bind     1607..1625
                     /label=poseq-2F
     primer_bind     complement(1773..1791)
                     /label=55C-1R
     primer_bind     2082..2100
                     /label=55seq-1F
     misc_feature    2199..2237
                     /label=Feature 11
     primer_bind     2218..2229
                     /label=55-2R
     primer_bind     2218..2229
                     /label=55-1R
     primer_bind     complement(2219..2256)
                     /label=55-1R
     primer_bind     2219..2256
                     /label=55-1F
     primer_bind     complement(2219..2237)
                     /label=55-2R
     primer_bind     2225..2256
                     /label=55-2F
     primer_bind     complement(2313..2331)
                     /label=55seq-1R
     primer_bind     2334..2352
                     /label=poseq-4F
     primer_bind     2939..2957
                     /label=poseq-5F
     primer_bind     3500..3524
                     /label=poseq-6F
     primer_bind     complement(3887..3916)
                     /label=LNS051-1R
     primer_bind     3896..3946
                     /label=LNS051-2F
     primer_bind     complement(4010..4029)
                     /label=pXYFPN-2-R (WTP142)
     primer_bind     complement(4097..4115)
                     /label=43SEQ-1R
     misc_feature    4219..4833
                     /standard_name="rep"
                     /label=rep
     misc_feature    5231..6046
                     /standard_name="nptI"
                     /label=nptI
                     /label="Kan"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 gtgcggcgcc gaccatgatg gacgagctga agtccatctc ggactcctgg ctttcgcatc
      541 acgccggcgg cgaaaagagc ttctcgatgg ggggctttga cccacgttat gtggccgagt
      601 tccccgtcgc ggttgttcgc agtgaaggca agatcgtcgc cttcgccacg ctgtggacga
      661 cggccgccaa gacggcgttc tccatggacc tgatgcgcta ctctgacgaa gcgcccaaga
      721 acgtcatgga ctatctgttc gtcgagctgc tgcagtgggg caaggacgag ggatacagcg
      781 ccttcgagtt cggcgtcgcg ccgctggcgg gtctgcagga tcgccctctg gccccgatca
      841 tgtcgcgcgt cggccggctg ctctacgagc gcggcgagga gatctataac ttccagggtg
      901 tgcgtcgcta caaggacaag tacgacccgg tctggcagcc gcgctacatt gccgcgcctc
      961 agaagtgggc gatcccgttc ctgctcgccg acatcgggct gctgtcgtcc ggtggggtct
     1021 ccggcctggc gaaacgaccc aagaagctcc cggagaaggc cgcgacctag tagcgcgtct
     1081 ggttacccgt ctggacgccg agcaggttca ccagatgcac cagcatcagc atcagtccga
     1141 ccacgcagat catcatcatc ggccgccaag ggatcagccg cgggcccttg agaatattga
     1201 tcggacgcgc gcccagccag ccgaagagca cggcgaggat caggaacagg atcgcggcgg
     1261 cggcggtgac gggcaggtcc atgcgcgctt ctaggcctgc gacaatccgc ctggcaaatg
     1321 gttaaccagt gttaaccctc ttgcttttga accgcgctac agagtgatgg gactctgtcc
     1381 tgattcctcc acagtgaatc gcacagccac tacatctggc gttttcgtcg cgtttacacc
     1441 ctaggaatcg gtgactagcg ttttccccag gtgcggggag atcgattcgc atgacggcgg
     1501 cttcgccatg gagcgttaag gggattgacc ccaaggcacg ggaggtcgcg aaagacctcg
     1561 cgcgtcgctc cggcatgact ttgggtgaat ggctgaaccg aatgatcatc gaaggcgatg
     1621 gtcagaccgc tgatccgcgt ctcgcggggg acgatgtccc caaccgcgcc tatctcgaga
     1681 ttgtcaaaga tgatgcgccg ccgcgcatcg agatcgccga gcatccggca gatgaggttg
     1741 gtcgcgtcgc gctggcgctg gatcgcctga cccaacgcat tgaagccgcc gaggggcgca
     1801 acgccgccgc catcaccggc atcgatcact ccgtccgtga cgccctgacg cgtctgggcg
     1861 catccgaacg tgagcagatc gccgtcgccg cccggttcga gggcgctgtc gacgaactga
     1921 agaccgagca ggctcgggcc accgaacgtc tgcgccggat cgagagcgag gcggcgggtc
     1981 cgcgctcggc cgaggcgttg cgcgcgcttg aaggcgcctt gggcaaggtc gccggacacc
     2041 tttacgaagg cgaggcgcgg acgcgcgagg cgatcgcgac cctggaagcc aagctgaacc
     2101 agcagagctc gggcgatccc agcgcgctgg tcgaggctgt ggtcgcgcga ctgggcgaac
     2161 gcctcgaagc cgccgaaacc cgcaccagcg acgcgctgcg cgaactgggc gcgtcgttcc
     2221 aggccctgga tcagcgccag gaactggtcg accgcatccg ccagagcgaa gagcgcacgc
     2281 tccgcatgct ggaagaagcg cgcgagaaga tcgacagccg tctgtccgag gcgcagcgga
     2341 agctggaagc tgcgccgccg tcgccgccgc ccgcgcaggc tccggcgccg gttgcgactg
     2401 ctcagcgtcc ggttccgccg gcggcttcgc ccttcgagga caactatttc agccaggccg
     2461 cctcgttcag cacgtctgag gatgaggctg acgcgtttga tgcgccgcct gcgccggccc
     2521 gtagcttcga ggtggcggag ttcccggctg cggagccgga agaaccggcc ttcgcacacg
     2581 atgactacgc gatcgccgat ggcttcgagc cggaaagccc gcgctatgaa gtcgagcctg
     2641 aggtctccga cttcgcgccc gccgagccgt cgcgtccgat gtcgacccgc gatatcatcg
     2701 agcaggcccg cgccgccgcg cgcgccgccg ccgcctcgga aggcaagggc ggcaaggcca
     2761 agtcggcgaa gaaggaaaag gcttccaagg ccagcggctc gctgttcagc ggctttggcg
     2821 gtttctcgac caagaagtcg aaggcgcgct tgggcgcgac cgtgacgacg gccctcgttg
     2881 tcttcgccgc cgccggcgcg ctcggggccg gcgtgggcgg cctgctgctg ctgaacaccg
     2941 acgacggcaa caactcgccc agccgcgtcg cccaggcgat cgctggtcgc aaggccgatg
     3001 tcgaggtgaa tggtcccgag gctgacacga cccccggcgc gccgcgcgcc gccgtcgcgc
     3061 tgacgacggg caaggtcgtc ccagccgagg tcgaggcccc tgcagccccg ccgaccaacg
     3121 aagccaaggc cctgttcgag gacgcggtcc gtaagatcga gtcgggggac cgctcgggcg
     3181 tcgagttgct gaagcgcgcc gccaatggcg gctacccggc cgcgcagttc tacctctcga
     3241 agatgtacga gggcggcaag aacggcgtga aggtcgatat ggccgaagcg cgccgctgga
     3301 gtgagcgcgc cgccaacggt ggcgatccgc gggccatgca caacctggcc ctctactact
     3361 tcaagggcga gggcggtccg cgcaattcga cgacggccgc cagctggttc cgcaaggccg
     3421 ccgacatggg cctggtcgac agccagttca acctggctca gctctacgaa agcggactgg
     3481 gcgtcagcca gaatccggcc gaagcctaca aatggtatgt gatcgccggc cgcgctgggg
     3541 attcgaccgc gcggggccgc gccacggcgc tgcgctcgca gctgaccgca gaggcccagc
     3601 agaccgctga ccgctcggcc ttggccttcc gtccgcagac tcaggtccag accgccagtc
     3661 tgtcgtccgc tgcgccggcc gccgcaaacg ccaaccttgg cgttgcgcag cgtgtcctgt
     3721 cgcagctggg ctattaccag gggccgcgcg acggtgtctc gtcgcccgcc ctgcgcatgg
     3781 cgatcgcggc ctatcagcgt gatcagggtc tgccccccac cggatcggtg gacgccgaga
     3841 ccctcaatcg cctgtcggtc tacgcgcgct aaacgcctcg cgacctcgcg ctttgccggg
     3901 aaggggcctt aattaatatg catggtacct taagatctcg agctccggag aattcgaacg
     3961 ttacgcgtac cggtgctagc tgcagcccgg gggatccact agttctagag cggccattca
     4021 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4081 cttgcagcac atcccccttt cgccagcggt atcagctcac tcaaaggcgg taatacggtt
     4141 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4201 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4261 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4681 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4741 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4861 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4921 ctagatcctt ttgggggggg ggggcgctga ggtctgcctc gtgaagaagg tgttgctgac
     4981 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg
     5041 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg
     5101 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt
     5161 caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac aaccaattaa
     5221 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     5281 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     5341 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     5401 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     5461 gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt
     5521 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     5581 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     5641 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     5701 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     5761 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     5821 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     5881 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     5941 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     6001 ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     6061 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     6121 tgtaacatca gagattttga gacacaacgt ggctttcccc cccccccc
//
