LOCUS       pLN058        6198 bp DNA     circular SYN 15-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   111111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    299..358
                     /standard_name="rrnBT1T2"
                     /label=rrnBT1T2
     primer_bind     complement(308..321)
                     /label=LNseq-1F
     primer_bind     321..339
                     /label=LNseq-1F
     primer_bind     333..358
                     /label=41SEQ-3F
     primer_bind     complement(432..490)
                     /label=LNS051-2R
     primer_bind     471..498
                     /label=LNS051-1F
     misc_feature    481..3935
                     /label=Feature 10
     gene            481..1070
                     /locus_tag="CCNA_02127"
                     /label=CCNA_02127
                     /db_xref="GeneID:7330433"
     CDS             481..1070
                     /codon_start=1
                     /locus_tag="CCNA_02127"
                     /product="bifunctional lysylphosphatidylglycerol 
                     flippase/synthetase MprF"
                     /label=CCNA_02127
                     /note="CC_2047"
                     /db_xref="GeneID:7330433"
                     /experiment="EXISTENCE:LC-MS"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517500.1"
                     /translation="AAPTMMDELKSISDSWLSHHAGGEKSFSMGGFDPRYVAEFPVAVV
                     RSEGKIVAFATLWTTAAKTAFSMDLMRYSDEAPKNVMDYLFVELLQWGKDEGYSAFEFG
                     VAPLAGLQDRPLAPIMSRVGRLLYERGEEIYNFQGVRRYKDKYDPVWQPRYIAAPQKWA
                     IPFLLADIGLLSSGGVSGLAKRPKKLPEKAAT"
     primer_bind     962..980
                     /label=poseq-1F
     gene            complement(1067..1282)
                     /locus_tag="CCNA_02126"
                     /label=CCNA_02126
                     /db_xref="GeneID:7330432"
     CDS             complement(1067..1282)
                     /codon_start=1
                     /locus_tag="CCNA_02126"
                     /product="hypothetical protein"
                     /label=CCNA_02126
                     /note="CC_2046"
                     /db_xref="GeneID:7330432"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517499.1"
                     /translation="MDLPVTAAAAILFLILAVLFGWLGARPINILKGPRLIPWRPMMMI
                     CVVGLMLMLVHLVNLLGVQTGNQTRY"
     primer_bind     complement(1111..1129)
                     /label=poseq-1R
     gene            1491..3902
                     /gene="podJ"
                     /locus_tag="CCNA_02125"
                     /label=podJ
                     /db_xref="GeneID:7330431"
     CDS             1491..3902
                     /codon_start=1
                     /gene="podJ"
                     /locus_tag="CCNA_02125"
                     /product="polar development protein podJ"
                     /label=podJ
                     /note="CC_2045"
                     /db_xref="GeneID:7330431"
                     /experiment="EXISTENCE:LC-MS"
                     /experiment="EXISTENCE:Ribosome profiling"
                     /protein_id="YP_002517498.1"
                     /translation="MTAASPWSVKGIDPKAREVAKDLARRSGMTLGEWLNRMIIEGDGQ
                     TADPRLAGDDVPNRAYLEIVKDDAPPRIEIAEHPADEVGRVALALDRLTQRIEAAEGRN
                     AAAITGIDHSVRDALTRLGASEREQIAVAARFEGAVDELKTEQARATERLRRIESEAAG
                     PRSAEALRALEGALGKVAGHLYEGEARTREAIATLEAKLNQQSSGDPSALVEAVVARLG
                     ERLEAAETRTSDALRELGASFQALDQRLGAVETANPATGVQEGLDSLAATLTQKMEAAR
                     LEMAAKLRESADGRFDRMERKLGEMAAHVQAAEQRSAQAIERMGREIVGVADAFNRRVH
                     AAESRNASAIEQVGGEVARIAASVEHKLNRADSVQAQALEKLGGEIARITEKLAERIGS
                     AERRNALAIDDVGEQVARVTERLNQRHERSSGKAKSAKKEKASKASGSLFSGFGGFSTK
                     KSKARLGATVTTALVVFAAAGALGAGVGGLLLLNTDDGNNSPSRVAQAIAGRKADVEVN
                     GPEADTTPGAPRAAVALTTGKVVPAEVEAPAAPPTNEAKALFEDAVRKIESGDRSGVEL
                     LKRAANGGYPAAQFYLSKMYEGGKNGVKVDMAEARRWSERAANGGDPRAMHNLALYYFK
                     GEGGPRNSTTAASWFRKAADMGLVDSQFNLAQLYESGLGVSQNPAEAYKWYVIAGRAGD
                     STARGRATALRSQLTAEAQQTADRSALAFRPQTQVQTASLSSAAPAAANANLGVAQRVL
                     SQLGYYQGPRDGVSSPALRMAIAAYQRDQGLPPTGSVDAETLNRLSVYAR"
     primer_bind     1607..1625
                     /label=poseq-2F
     primer_bind     2082..2100
                     /label=Primer 1
     primer_bind     2246..2264
                     /label=poseq-3F
     primer_bind     2705..2723
                     /label=58SEQ-1F
     primer_bind     complement(2760..2785)
                     /label=LNS058-1R
     misc_feature    2761..2780
                     /label=Feature 11
     primer_bind     2776..2799
                     /label=LNS058-1F
     primer_bind     complement(2961..2979)
                     /label=58SEQ-1R
     primer_bind     2969..2987
                     /label=poseq-5F
     primer_bind     3530..3554
                     /label=poseq-6F
     primer_bind     complement(3917..3946)
                     /label=LNS051-1R
     primer_bind     3926..3976
                     /label=LNS051-2F
     primer_bind     complement(4049..4067)
                     /label=LNseq-1R
     primer_bind     complement(4127..4145)
                     /label=43SEQ-1R
     misc_feature    4249..4863
                     /standard_name="rep"
                     /label=rep
     misc_feature    5261..6076
                     /standard_name="nptI"
                     /label=nptI
                     /label="Kan"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 gtgcggcgcc gaccatgatg gacgagctga agtccatctc ggactcctgg ctttcgcatc
      541 acgccggcgg cgaaaagagc ttctcgatgg ggggctttga cccacgttat gtggccgagt
      601 tccccgtcgc ggttgttcgc agtgaaggca agatcgtcgc cttcgccacg ctgtggacga
      661 cggccgccaa gacggcgttc tccatggacc tgatgcgcta ctctgacgaa gcgcccaaga
      721 acgtcatgga ctatctgttc gtcgagctgc tgcagtgggg caaggacgag ggatacagcg
      781 ccttcgagtt cggcgtcgcg ccgctggcgg gtctgcagga tcgccctctg gccccgatca
      841 tgtcgcgcgt cggccggctg ctctacgagc gcggcgagga gatctataac ttccagggtg
      901 tgcgtcgcta caaggacaag tacgacccgg tctggcagcc gcgctacatt gccgcgcctc
      961 agaagtgggc gatcccgttc ctgctcgccg acatcgggct gctgtcgtcc ggtggggtct
     1021 ccggcctggc gaaacgaccc aagaagctcc cggagaaggc cgcgacctag tagcgcgtct
     1081 ggttacccgt ctggacgccg agcaggttca ccagatgcac cagcatcagc atcagtccga
     1141 ccacgcagat catcatcatc ggccgccaag ggatcagccg cgggcccttg agaatattga
     1201 tcggacgcgc gcccagccag ccgaagagca cggcgaggat caggaacagg atcgcggcgg
     1261 cggcggtgac gggcaggtcc atgcgcgctt ctaggcctgc gacaatccgc ctggcaaatg
     1321 gttaaccagt gttaaccctc ttgcttttga accgcgctac agagtgatgg gactctgtcc
     1381 tgattcctcc acagtgaatc gcacagccac tacatctggc gttttcgtcg cgtttacacc
     1441 ctaggaatcg gtgactagcg ttttccccag gtgcggggag atcgattcgc atgacggcgg
     1501 cttcgccatg gagcgttaag gggattgacc ccaaggcacg ggaggtcgcg aaagacctcg
     1561 cgcgtcgctc cggcatgact ttgggtgaat ggctgaaccg aatgatcatc gaaggcgatg
     1621 gtcagaccgc tgatccgcgt ctcgcggggg acgatgtccc caaccgcgcc tatctcgaga
     1681 ttgtcaaaga tgatgcgccg ccgcgcatcg agatcgccga gcatccggca gatgaggttg
     1741 gtcgcgtcgc gctggcgctg gatcgcctga cccaacgcat tgaagccgcc gaggggcgca
     1801 acgccgccgc catcaccggc atcgatcact ccgtccgtga cgccctgacg cgtctgggcg
     1861 catccgaacg tgagcagatc gccgtcgccg cccggttcga gggcgctgtc gacgaactga
     1921 agaccgagca ggctcgggcc accgaacgtc tgcgccggat cgagagcgag gcggcgggtc
     1981 cgcgctcggc cgaggcgttg cgcgcgcttg aaggcgcctt gggcaaggtc gccggacacc
     2041 tttacgaagg cgaggcgcgg acgcgcgagg cgatcgcgac cctggaagcc aagctgaacc
     2101 agcagagctc gggcgatccc agcgcgctgg tcgaggctgt ggtcgcgcga ctgggcgaac
     2161 gcctcgaagc cgccgaaacc cgcaccagcg acgcgctgcg cgaactgggc gcgtcgttcc
     2221 aggccctgga tcagcgcttg ggcgccgtcg agactgccaa tcccgccacg ggcgttcagg
     2281 agggcctgga cagcctggcc gccaccctca cgcagaagat ggaagccgct cgcctggaga
     2341 tggcggccaa gcttcgcgaa agcgccgacg gacgtttcga tcgcatggag cgcaagctcg
     2401 gcgagatggc ggcccacgtg caggcggccg aacagcgctc ggcccaggcc attgagcgta
     2461 tgggccgcga gattgtcggt gtcgctgatg cgttcaatcg ccgcgtccac gccgccgaga
     2521 gccgtaacgc cagcgctatc gagcaggtcg gtggcgaggt tgcgcggatc gccgccagcg
     2581 tcgagcacaa gctcaatcgc gccgatagcg ttcaagccca ggcccttgaa aagctgggcg
     2641 gcgagatcgc ccggatcacc gagaagctgg ccgagcggat cggcagcgcc gagcgacgca
     2701 acgccctggc catcgacgat gtcggcgaac aggtcgcccg cgtcacagag cgcctgaacc
     2761 aacgccacga acgttccagc ggcaaggcca agtcggcgaa gaaggaaaag gcttccaagg
     2821 ccagcggctc gctgttcagc ggctttggcg gtttctcgac caagaagtcg aaggcgcgct
     2881 tgggcgcgac cgtgacgacg gccctcgttg tcttcgccgc cgccggcgcg ctcggggccg
     2941 gcgtgggcgg cctgctgctg ctgaacaccg acgacggcaa caactcgccc agccgcgtcg
     3001 cccaggcgat cgctggtcgc aaggccgatg tcgaggtgaa tggtcccgag gctgacacga
     3061 cccccggcgc gccgcgcgcc gccgtcgcgc tgacgacggg caaggtcgtc ccagccgagg
     3121 tcgaggcccc tgcagccccg ccgaccaacg aagccaaggc cctgttcgag gacgcggtcc
     3181 gtaagatcga gtcgggggac cgctcgggcg tcgagttgct gaagcgcgcc gccaatggcg
     3241 gctacccggc cgcgcagttc tacctctcga agatgtacga gggcggcaag aacggcgtga
     3301 aggtcgatat ggccgaagcg cgccgctgga gtgagcgcgc cgccaacggt ggcgatccgc
     3361 gggccatgca caacctggcc ctctactact tcaagggcga gggcggtccg cgcaattcga
     3421 cgacggccgc cagctggttc cgcaaggccg ccgacatggg cctggtcgac agccagttca
     3481 acctggctca gctctacgaa agcggactgg gcgtcagcca gaatccggcc gaagcctaca
     3541 aatggtatgt gatcgccggc cgcgctgggg attcgaccgc gcggggccgc gccacggcgc
     3601 tgcgctcgca gctgaccgca gaggcccagc agaccgctga ccgctcggcc ttggccttcc
     3661 gtccgcagac tcaggtccag accgccagtc tgtcgtccgc tgcgccggcc gccgcaaacg
     3721 ccaaccttgg cgttgcgcag cgtgtcctgt cgcagctggg ctattaccag gggccgcgcg
     3781 acggtgtctc gtcgcccgcc ctgcgcatgg cgatcgcggc ctatcagcgt gatcagggtc
     3841 tgccccccac cggatcggtg gacgccgaga ccctcaatcg cctgtcggtc tacgcgcgct
     3901 aaacgcctcg cgacctcgcg ctttgccggg aaggggcctt aattaatatg catggtacct
     3961 taagatctcg agctccggag aattcgaacg ttacgcgtac cggtgctagc tgcagcccgg
     4021 gggatccact agttctagag cggccattca ctggccgtcg ttttacaacg tcgtgactgg
     4081 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagcggt
     4141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgggggggg ggggcgctga
     4981 ggtctgcctc gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc
     5041 agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg
     5101 attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga
     5161 tccttcaact cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg
     5221 taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca
     5281 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     5341 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     5401 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     5461 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     5521 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     5581 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     5641 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     5701 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     5761 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     5821 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     5881 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     5941 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     6001 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc
     6061 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     6121 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
     6181 ggctttcccc cccccccc
//
