LOCUS       Nppa-EGFP-1                 4840 bp    DNA     circular SYN 09-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(3867..4583)
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2824..3252)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2667..2771)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1806..2666)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(3801..3866)
                     /note="pLannotate"
                     /label="MCS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(1047..1635)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(3651..3706)
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(693..723)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(738..759)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(669..685)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(3257..3414)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     repeat_region   complement(3428..3558)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="92.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   500..562
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="44.7"
                     /fragment="True"
                     /other="repeat_region"
     CDS             complement(772..864)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   complement(580..607)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 tcagctgcaa gagtcacatt cttgctgatt tgcctcaaga ggcccccact tcaaaggtgt
       61 gagaggagca gaaaagagtc cttggttatc ttcctgggcg ctcgcctcag gattctttct
      121 ccctggcaac aggacccatg cagggaagca gagggtgacg gctggcctgc acccagctcc
      181 ccctgcttgc tgctcagctg tcaggggctc caaataaggg cgggagagga ggcaatgaca
      241 ggcagctggg aggccagcta ggctggccag ggagtacaca tgctggagct agcttcacac
      301 ctgattcatc ggcctcgtgg gtggacctct ggccccagac agcccacccc caccctagat
      361 gtccctgtta gcagtcaggt gaaagagcta ttattttttt tttagagtat gaagtcagcc
      421 aggaagccaa catgatacag ggcccggatc cccattaccc tggtagataa gtagcatggc
      481 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
      541 gctcgctcgc tcactgaggc cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
      601 cgcgcagcct aattaaggcc ttaattaaat ctggcgtaat catggtcata gctgtttcct
      661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1921 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2761 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     2821 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2881 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2941 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3001 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3061 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     3121 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3181 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3241 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     3301 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     3361 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattaggtt
     3421 aattaaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg
     3481 cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact
     3541 ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta tctaccaggg
     3601 taatgggtat tatgggtggt cgaaattcag atcaacgaat tgcttccgag tgagagacac
     3661 aaaaaattcc aacacactat tgcaatgaaa ataaatttcc tttattagcc agaagtcaga
     3721 tgctcaaggg gcttcatgat gtccccataa tttttggcag agggaaaaag atcggatcgg
     3781 gtaccttatc tagatccggt ggatcccggg cccgcggtac cgtcgactgc agaattcgaa
     3841 gcttgagctc gagatctgag tccggacttg tacagctcgt ccatgccgag agtgatcccg
     3901 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
     3961 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     4021 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
     4081 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
     4141 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     4201 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     4261 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     4321 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
     4381 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     4441 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     4501 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     4561 agctcctcgc ccttgctcac catgaattct ctagaccatg gcaccttcct cttcttcttg
     4621 ggcatggtgt ctcctagctg ccagcatctc ccgtttttat agccccccag ctctttgaga
     4681 aggcaggggg gctagaaccc tccccattct gtcacttgca gcgataaagc gatggagtga
     4741 ggagccagca gaagatgccc ttttaaagtt atcagttcgg caaagcttgt cacggcccct
     4801 ttgccttgag cagctccact cacccccctc ccccagaccc
//
