LOCUS       Exported                7353 bp ds-DNA    circular SYN 20-9ÔÂ-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    enDelIscB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported 2025Äê9ÔÂ20ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        295..572
                     /note="chicken beta-actin promoter"
     misc_feature    573..801
                     /note="Hyrid Intron"
     misc_feature    807..812
                     /note="Feature 22"
     misc_feature    813..818
                     /note="Kozak sequence"
     misc_feature    819..821
                     /note="Kozak"
     CDS             822..2189
                     /codon_start=1
                     /note="enDelIscB"
                     /translation="SRVLVVDADRRPLAPCTPRRARLLLNSGKAAVLRRYPFTIILKQS
                     YPTASPRPVRLKLDPGSKTTGIAVVTEATGEVVWAAELQHRGQLIKNALEKRRALRRSR
                     RNRKTRYRPARFLNRKRTGPPLLSSADTVSTLGKWLPPSLQHRIEVIMTWVHRLRRYLP
                     ITAISQEIVRFDMQKMQNPEISGVEYQQGTLFGYEVREYLLDKWRRQCGYCGAKDKRLE
                     VDHIVPRSHGGSDRVSNLTLSCEPCNKRKNQRPAAVFLAKKPEVLQKLQRQAKAPLKDA
                     AAVNSTRYALLERLKATGLPVEVASGGRTKFNRSRRQIPKTHWLDAACVGASTPEVLKW
                     EAVRPLAIKAMGHGKRQVVNVDAYGFPRGKAKGIPVHPFRTGDIVRAEIPKGKYKGTYV
                     SRIAETTTSKPLAGFKSKTGKRIQCHTKHMTKLFNRDGYGYGFLKAPEPRQTVISES"
     variation       852..854
                     /note="C12R"
     variation       1107..1109
                     /note="S97K"
     variation       1116..1118
                     /note="S100A"
     variation       1128..1130
                     /note="G104S"
     variation       1167..1169
                     /note="W117F"
     misc_feature    1242..1244
                     /note="A142P"
     variation       1767..1769
                     /note="E317R"
     variation       1836..1838
                     /note="Q340K"
     variation       2004..2006
                     /note="V396K"
     misc_feature    2124..2126
                     /note="S436R"
     CDS             2190..2237
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    2246..2269
                     /note="i16-2"
     polyA_signal    2271..2446
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    2447..2729
                     /note="F3"
     promoter        2479..2728
                     /label="U6 Promoter"
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2729
                     /note="replace"
     misc_feature    2729
                     /note="Bsa1"
     misc_feature    2730..3012
                     /note="F4"
     misc_feature    2730..2750
                     /note="Bsa1"
     misc_feature    2730..2748
                     /note="replace"
     misc_feature    2751..2998
                     /note="Delta-35-41+GAAA+T205A+Delta-221-240+GAAA"
     misc_feature    2751..2769
                     /note="DR"
     misc_feature    2770..2773
                     /note="LOOP"
     misc_feature    2774..2804
                     /note="anti-repeat"
     misc_feature    2952
                     /note="T205A"
     misc_feature    3013..3018
                     /note="Feature 21"
     enhancer        3079..3382
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3383..3586
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3615..4325
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             4046
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation=""
     rep_origin      4880..5335
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5617..5721
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             5722..6582
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6753..7341
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggtacccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
       61 gcccattgac gtcaatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      121 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      181 ctattgacgt caatgacggt aaatggcccg cctggcattg tgcccagtac atgaccttat
      241 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
      301 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
      361 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg
      421 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
      481 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
      541 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgacgctg ccttcgcccc
      601 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      661 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagct gagcaagagg
      721 taagggttta agggatggtt ggttggtggg gtattaatgt ttaattacct ggagcacctg
      781 cctgaaatca ctttttttca ggttggaccg gtgccaccat gagcagagtg ctggtggtgg
      841 atgctgatag gcgccctctg gccccttgca cccctcggag agccagactg ctgctgaatt
      901 ctggcaaagc tgccgtgctg agacggtacc ctttcaccat catcctgaag cagagctacc
      961 ccaccgccag ccctagaccc gtgcggctga agctggaccc cggaagcaag accaccggca
     1021 tcgccgtggt gacagaggcc acaggagaag tggtgtgggc cgctgagctg cagcacagag
     1081 gccagctgat caagaacgcc ctggaaaaga gaagagccct gcggcgcagc cggcgaaacc
     1141 ggaagacacg gtatagacct gccagattcc tgaatagaaa gagaacagga cctccactgc
     1201 tgagcagcgc cgacactgtg tctaccctgg gcaagtggtt gcccccgagc ctgcagcata
     1261 ggatcgaggt gatcatgacc tgggtccaca gactgagaag atacctgccc atcaccgcca
     1321 tttctcagga gatcgtgaga ttcgacatgc aaaagatgca gaaccccgaa atcagcggcg
     1381 tggaatacca gcagggcacc ctcttcggct acgaggtgcg ggaatacctc ctggacaagt
     1441 ggcggagaca atgtggctac tgcggcgcta aggacaagag acttgaggtc gaccacatcg
     1501 ttcctcggag ccacggcggc agcgacagag tgtccaacct gacactgtcc tgcgagcctt
     1561 gcaacaaaag aaagaaccag aggccagctg ctgtgttcct ggctaaaaag cccgaggtcc
     1621 tgcagaagct gcagcggcag gccaaggccc ctctgaagga cgccgccgcc gtgaacagca
     1681 cccgttacgc cctgctggaa agactgaagg ccaccggcct gcctgtggaa gtggccagcg
     1741 gcggaagaac caagttcaat agaagcagga gacagatccc caagacccac tggctggatg
     1801 ccgcctgcgt gggcgcttct acacctgagg tgctgaagtg ggaggccgtt agaccactgg
     1861 ccatcaaggc catgggccac ggcaaacggc aggtggtcaa cgtggacgcc tacggatttc
     1921 ctcggggcaa ggccaaaggc atccctgtgc accccttcag aaccggagat atcgtgcggg
     1981 ccgagattcc aaagggcaaa tacaagggaa catacgtgtc cagaatcgcc gagacaacca
     2041 catctaagcc tctagcaggc tttaagagca agaccggcaa gcggatccag tgtcacacca
     2101 agcacatgac aaagctgttc aaccgcgacg gctatggcta cggcttcctg aaagcccctg
     2161 aacctagaca gaccgtgatc agcgagtcta aaaggccggc ggccacgaag aaggccggcc
     2221 aggcaaagaa gaaaaagtaa gaattcctag agctcgctga tcagcctcga ctgtgccttc
     2281 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     2341 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     2401 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa
     2461 tagcaggcat gctggggaga gggcctattt cccatgattc cttcatattt gcatatacga
     2521 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     2581 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     2641 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2701 tatatcttgt ggaaaggacg aaacaccggg gagaccgaga gagggtctca gttgtagcct
     2761 ccactcattg aaaaatgagt gcgggaaacc gcagctacaa ccagactaag ttctttggga
     2821 actacgttat tgtcgtcatc atacccacgg gtgcgtgcca gcctgtggct ctatggtcgt
     2881 gcattaaaca gggactttgg agggtgccca gtgtgtacgg cgtcaaaagc ggcgataaca
     2941 ttgtcgaggc aaactttacg tgacggcgaa agcccgatcg ctagctttaa ggctaatttt
     3001 ttattttttt gcggccgcta gttattaata gtaatcaatt acggggtcat tagttcatag
     3061 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3361 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3421 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3481 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     3541 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     3601 tcagatccgc tagcatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     3661 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     3721 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     3781 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     3841 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     3901 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     3961 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     4021 ccgacggccc cgtaatgcag aagaaaacca tgggctggga ggcctcctcc gagcggatgt
     4081 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     4141 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     4201 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     4261 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     4321 agtaaggatc cgggatgcag aaattgatga tctattaaac aataaagatg tccactaaaa
     4381 tggaagtttt tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga
     4441 atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat gcctgctctt
     4501 tactgaaggc tctttactat tgctttatga taatgtttca tagttggata tcataattta
     4561 aacaagcaaa accaaattaa gggccagctc attcctccca ctcatgatct atagatctat
     4621 agatctctcg tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt
     4681 ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca
     4741 tacacttcat tctcagtatt gttttgccaa gttctaattc catcagaagc tggtcgacct
     4801 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4861 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4921 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4981 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5041 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     5101 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5161 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5221 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5281 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     5341 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5401 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5461 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5521 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5581 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5641 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5701 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5761 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5821 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5881 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5941 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6001 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6061 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6121 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6181 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6241 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6301 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6361 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6421 ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc
     6481 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6541 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6601 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6661 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6721 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6781 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6841 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6901 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6961 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7021 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7081 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7141 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7201 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7261 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7321 ggggggcgga gcctatggaa aaacgccagc aac
//
